Ilmu dan Teknologi di Pelayanan Kesehatan Primer: dari Penyakit Tropik Terabaikan Sampai Personalized Medicine

No Abstrac

Lost to Follow-up Among Patients who Underwent Vasectomy reversal with Double Layer Microscopic Techniques in 2011-2015

Vasectomy already been accepted by the society as easy and effective method for male contraceptive. However, some patients want to restore their fertility status due to divorce or re-marriage cases. Techniques in performing vasectomy reversal are varying with their own advantages and disadvantages. One of the techniques is double layer microscopy vasectomy reversal. We evaluate the success rate of this technique based on the semen analysis. The success rate was good with around 98.5% patients with complete follow up had sperm in their semen analysis[MP1] . However, total success of follow up were very low (5 out of 19 patients) even though the cost of reversal vasectomy was quite expensive (around 3.000$USD). Lost to follow up rate of reversal vasectomy was 26%

Cytotoxic and antibacterial activities of marine sponge-derived fungus aspergillus nomius NC06

Sponge-derived fungi have attracted recent attention due to its important source of interesting biologically active compounds. In our previous study, we have obtained 13 fungi from marine sponge Neopetrsiachaliniformis. Among them, only Aspergillus nomius NC06 showed cytotoxic activity with the percentage of viability113.9 % and 70.31 % of Vero cell and WiDr colon cancer cell, respectively. This study aimed to isolate the cytotoxic compound from the ethyl acetate extract of N. nomius NC06 using chromatography method. A total of 5 fractions of the extract obtained using vacuum liquid chromatography. These fractions were tested against HCT 116 colon cancer cell and ten human pathogenic bacteria. Fraction II, III, IV, and V showed cytotoxic activity with IC50 of 5.28, 15.82, 10.27, and 45.57 µg/mL, respectively. In antibacterial testing, fraction II and III were potential because of their ability to inhibit the growth of ten pathogenic bacteria with the diameter of inhibition zone more than 12 m

Y-Chromosomal Microdeletion in Idiopathic Azoospermic and Severe Oligozoospermic Indonesian Men

Aim: to detect Y-chromosomal microdeletion in Indonesian men with azoospermia or severe oligozoospermia using multiplex PCR. Methods: we performed 2 multiplex PCR amplifications of the Azoospermia Factor (AZF) region in 71 men. Criteria for including a patient were fulfilled if they presented with azoospermia or severe oligozoospermia, with or without additional abnormalities of sperm motility or of head morphology, raised or normal levels of FSH, normal levels of LH and testosterone, and with no evidence of testicular tumors or other abnormalities. Five men participated as control persons. Results: partial deletion of AZFa was found in 11 men (15.49%), complete deletion of AZFb in 1 man (1.4%), and complete deletion of AZFc in 1 man (1.4%). The unspecific type of deletion was also detected, including the DBY gene in 2 men (2.81%), and partial deletion of both AZFa and AZFb in 2 men (2.81%). No AZF deletion was observed in the control probands. Related to the type of deletion, the AZFa and AZFb deletion showed spermatogenesis arrest in most tubules, while deletion of the DBY gene is associated with the sertoli cell only (SCO) syndrome. Conclusion: the frequency of partial deletion of AZFa was found to be relatively high in our center. The type of deletion is associated with the testicular histology

The Role of Varicocele Repair in Non-Obstructive Azoospermic Men: A Systematic Review

The outcomes of varicocele repair in non-obstructive azoospermic men remain the subject of controversy. Until now, small studies with small number of patients performed make it difficult to assess the efficacy of varicocele surgery in men with non-obstructive azoospermia. This review is performed to evaluate quality of the sperm among non-obstructive azoospermic men after varicocele repair.

Efficacy and tolerability of vardenafil in Asian men with erectile dysfunction

10.1111/j.1745-7262.2008.00388.xAsian Journal of Andrology103495-50

Ilmu dan Teknologi di Pelayanan Kesehatan Primer: dari Penyakit Tropik Terabaikan Sampai Personalized Medicine

No Abstrac

Karakteristik Fisik, Kimia, Dan Biologi Tempat Perindukan Potensial Larva Anopheles Sp. Dan Indeks Habitat Di Desa Sukamaju Kecamatan Punduh Pidada Kabupaten Pesawaran

ABSTRACT: PHYSICAL, CHEMICAL, AND BIOLOGICAL CHARACTERISTICS OF POTENTIAL FARMING PLACE OF LARVA Anopheles sp. AND HABITAT INDEX IN SUKAMAJU VILLAGE DISTRICT PUNDUH PIDADA PESAWARAN DISTRICT Background:  Malaria is an infectious disease transmitted through mosquitoes and has become a health problem both in the world and Indonesia especially in Lampung. The population of the malaria vector is strongly influenced by the location of the breeding place. Purpose: Knowing the Physical, Chemical, and Biological Characteristics of Potential Breeding Places for Anopheles Sp. and Habitat Index in Sukamaju Village, Punduh District, Pesawaran Regency, Lampung Province, 2020. Methods: This was a descriptive study with a cross-sectional study research design. The physical characteristics have done by measuring temperature and water depth, the chemical characteristics by measuring pH and water salinity, and the biological characteristics by looking at organisms found at the sampling site. As well as calculating the habitat index. Results: The physical characteristics of the brooding sites have an average water temperature of 31.33 ° C and an average water depth of 25.840 cm. Chemical characteristics of brooding sites mean 2.4280 ‰ of water salinity, and most of the acidity (pH) of water is acidic. The biological characteristics of breeding sites are mostly water and plant predators. Habitat index obtained a value of 1%.Conclusion: That these places have the potential to transmit malaria. Keywords: Anopheles sp., breeding place, malaria   INTISARI: KARAKTERISTIK FISIK, KIMIA, DAN BIOLOGI TEMPAT PERINDUKAN POTENSIAL LARVA Anopheles sp. DAN INDEKS HABITAT DI DESA SUKAMAJU  KECAMATAN PUNDUH PIDADA KABUPATEN PESAWARAN  Latar Belakang : Malaria merupakan penyakit infeksi yang ditularkan melalui nyamuk dan telah menjadi masalah kesehatan baik di dunia maupun di Indonesia khususnya di daerah Lampung. Populasi vektor malaria sangat dipengaruhi oleh lokasi tempat perindukannya. Tujuan : Mengetahui Karakteristik Fisik, Kimia, dan Biologi Tempat Perindukan Potensial Nyamuk Anopheles Sp. dan Indeks Habitat Di Desa Sukamaju Kecamatan Punduh Kabupaten Pesawaran Provinsi Lampung Tahun 2020. Metode : Jenis penelitian ini merupakan penelitian deskriptif dengan pendekatan cross sectional. Karakteristik fisik dilakukan dengan mekukur suhu dan kedalaman air, karakteristik kimia dengan mengukur pH dan salinitas air, dan karakteristik biologi dengan melihat organisme yang ditemukan di tempat pengambilan sampel. Serta melakukan penghitungan indeks habitat. Hasil : Karakteristik fisik pada tempat perindukan rata-rata suhu air 31,33°C, dan rata-rata kedalaman air 25,840 cm. Karakteristik Kimia pada tempat perindukan rata-rata salinitas air 2,4280‰, dan sebagian besar derajat keasaman (pH) air adalah asam. Karakteristik Biologi pada tempat perindukan sebagian besar terdapat predator air dan tumbuhan. Indeks habitat didapatkan nilai 1%. Kesimpulan : Bahwa tempat-tempat tersebut berpotensi untuk penularan penyakit malaria. Kata Kunci : Anopheles sp., malaria, tempat perinduka

Uji klinis: Landasan teori dan panduan praktis

viii, 334 hal; 23,5 c

Inhibition of Bile Acid Accumulation Decreased the Excessive Hepatocyte Apoptosis and Improved the Liver Secretion Functions on Obstructive Jaundice Patients

Excessive hepatocyte apoptosisinduced by bile acid accumulation occurred in severe obstructive jaundice, and impair the liver secretion function. The objective of this study is to determine whether the inhibition of bile acid accumulation through bile duct decompression affect the excessive  hepatocyte apoptosis and caused improvement the liver secretion functions on human model. In this study we use a before and after study on severe obstructive jaundice patients due to extra hepatic bile duct tumor was  decompressed. Bile duct decompression was performed as a model of the role of inhibition of bile acid accumulation inhibition bile acid accumulation and excessive hepatocyte apoptosis. Bile acid and marker of liver secretion functions were serially measured. Liver biopsy pre and post decompression was performed for Hepatocyte apoptosis pathologic examination by TUNEL fluorescing, which measured by 2 people in double blinded system. Total bile acid, and liver secretion functions were measured by automated  chemistry analyzer. The result of this study shows that twenty one severe obstructive jaundice patients were included. After decompression the hepatocyte apoptosis index decreased from an average of 53.1 (SD 105) to 11.7 (SD 13.6) (p < 0.05). Average of bile acid serum decreased from 96.4 (SD 53.8) to 19.9 (SD 39.5) until 13.0 (SD 12.6) μmol/L (p < 0.05) Total bilirubin decreased from 20.0 (SD 8.9) to 13.3 (SD 5.0) until 6.2 (SD 4.0) mg/dL (p < 0.05), while the phosphates alkaline (ALP) and γ-glutamil transpeptidase (γ-GT) activities also decreased significantly. In conclusion, bile acids accumulation and excessive hepatocyte apoptosis through bile duct decompression improve the liver secretion functions by inhibition mechanism