An advanced draft genome assembly of a desi type chickpea (Cicer arietinum L.)

Chickpea (Cicer arietinum L.) is an important pulse legume crop. We previously reported a draft genome assembly of the desi chickpea cultivar ICC 4958. Here we report an advanced version of the ICC 4958 genome assembly (version 2.0) generated using additional sequence data and an improved genetic map. This resulted in 2.7-fold increase in the length of the pseudomolecules and substantial reduction of sequence gaps. The genome assembly covered more than 94% of the estimated gene space and predicted the presence of 30,257 protein-coding genes including 2230 and 133 genes encoding potential transcription factors (TF) and resistance gene homologs, respectively. Gene expression analysis identified several TF and chickpea-specific genes with tissue-specific expression and displayed functional diversification of the paralogous genes. Pairwise comparison of pseudomolecules in the desi (ICC 4958) and the earlier reported kabuli (CDC Frontier) chickpea assemblies showed an extensive local collinearity with incongruity in the placement of large sequence blocks along the linkage groups, apparently due to use of different genetic maps. Single nucleotide polymorphism (SNP)-based mining of intra-specific polymorphism identified more than four thousand SNPs differentiating a desi group and a kabuli group of chickpea genotypes

Detection, Isolation and Confirmation of Crimean-Congo Hemorrhagic Fever Virus in Human, Ticks and Animals in Ahmadabad, India, 2010–2011

A nosocomial outbreak of CCHFV occurred in January 2011, in a tertiary care hospital in Ahmadabad, Gujarat State in western India. Out of a total five cases reported, contact transmission occurred to three treating medical professionals, all of whom succumbed to the disease. The only survivor was the husband of the index case. These results highlight the importance of considering CCHFV as a potential aetiology for Hemorrhagic fever (HF) cases in India. This also underlines the need for strict barrier nursing and patient isolation while managing these patients. During the investigation presence of CCHFV RNA in Hyalomma anatolicum ticks and livestock were detected in the village from where the primary case (case A) was reported. Further retrospective investigation confirmed two CCHF human cases in Rajkot village 20 kilometres to the west of Ahmadabad in 2010, and CCHFV presence in the livestock 200 kilometres to the north in the neighbouring State Rajasthan. This report shows the presence of CCHFV in human, ticks and animals in Gujarat, India. The fact of concern is the spread of this disease from one state to another due to trading of livestock

An Investigation of Use of Information Sources by Social Scientists

This study is based on data collected through questionnaires given to social scientists of Mizoram University, a central university in India. Goals of the research were to investigate the use of formal and informal sources of information by social scientists; to discover the degree to which they make use of various types of information; methods used to communicate, and availability of information sources and services in Mizoram University

Bacterial Strategies to Preserve Cell Wall Integrity Against Environmental Threats

Bacterial cells are surrounded by an exoskeleton-like structure, the cell wall, composed primarily of the peptidoglycan (PG) sacculus. This structure is made up of glycan strands cross-linked by short peptides generating a covalent mesh that shapes bacteria and prevents their lysis due to their high internal osmotic pressure. Even though the PG is virtually universal in bacteria, there is a notable degree of diversity in its chemical structure. Modifications in both the sugars and peptides are known to be instrumental for bacteria to cope with diverse environmental challenges. In this review, we summarize and discuss the cell wall strategies to withstand biotic and abiotic environmental insults such as the effect of antibiotics targeting cell wall enzymes, predatory PG hydrolytic proteins, and PG signaling systems. Finally we will discuss the opportunities that species-specific PG variability might open to develop antimicrobial therapies

Flavone Glycoside Based Validated RP-LC Method for Quality Evaluation of Prishniparni (Uraria picta)

An LC method is developed for the quantitation of rhoifolin in Uraria picta, a plant of high usage frequency in all Asian traditional systems of medicine. An isocratic RP-LC method using C18 column, UV detection 265 nm and specificity with PDA and MS is speeding up, reliable and comprehensive analysis of rhoifolin in U. picta. Good linearity was obtained in the working range (0.02–0.10 mg mL-1), with correlation coefficients >0.99. LOD and LOQ were 2.33 and 7.69 ng, respectively. The method was validated following international guidelines. The described method can be utilized for assays and stability tests of U. picta extracts as well as Ayurvedic drugs based on Prishniparni

Synthesis of D-ring functionalized and D-benzo/hetero-annulated estrone derivatives via α-oxoketene dithioacetal

Synthesis and chemical transformation of the α-oxoketene dithioacetal, derived from estrone methyl ether, leading to new D-ring functionalized (4 and 6), D-benzo- (8), D-naphtho- (10 and 11) and D-hetero-annulated (12-14) derivatives are described

Stakeholders prioritization of climate-smart agriculture interventions: Evaluation of a framework

This paper presents a framework to prioritize locally suitable climate-smart agricultural (CSA) interventions and implementation suitability assessments with key stakeholders: state and district agriculture departments, extension offices, agriculture research institutions, NGOs and donor agencies, private sector and farmers. Prioritization of appropriate interventions for given contexts is needed to assist relevant stakeholders to make strategic decisions and improve adaptability and efficiency of agriculture production system in the face of climate change and variability. First step includes participatory identification and evaluation of location specific CSA interventions suitable for different crop and cropping system with potential to reduce climatic risks in agriculture. All CSA interventions were evaluated based on their contribution to increase farm productivity and income, building resilience to changing climate and reduction of agricultural emissions. Second step includes evaluation of overall implementation feasibility of selected CSA interventions based on their technical feasibility, cost of implementation, inclusivity and synergy with current government programs. In the third step, potential barriers of CSA technology adoption were assessed linking with availability of resources, farmers' knowledge and acceptability, access to agriculture extensions service, market and government support. In the fourth step, incentive mechanisms to promote CSA interventions such as government subsidy, market linkage, provision of loan and capacity building were evaluated with farmers and key stakeholders. Finally, this prioritization framework assessed the role of different organizations such as the government, private sector, non-government organizations, custom hiring centers and community based organizations in promoting CSA interventions at the local level. Results show that this framework provides ample space for local stakeholders to integrate their knowledge and experience on CSA interventions in prioritization and investment planning. Stakeholders prioritized mainly water and nutrient management technologies, agriculture insurance against climatic risks and ICT based weather and agro-advisory services. This framework provides a decision support tool for policymaking in adaptation and mitigation activities in agriculture sector at the local level. This framework can be used by governments, development organizations and the private sector for investment decision-making

Mapping of Functional Domains in Herpesvirus Saimiri Complement Control Protein Homolog: Complement Control Protein Domain 2 Is the Smallest Structural Unit Displaying Cofactor and Decay-Accelerating Activities▿

Herpesvirus saimiri encodes a functional homolog of human regulator-of-complement-activation proteins named CCPH that inactivates complement by accelerating the decay of C3 convertases and by serving as a cofactor in factor I-mediated inactivation of their subunits C3b and C4b. Here, we map the functional domains of CCPH. We demonstrate that short consensus repeat 2 (SCR2) is the minimum domain essential for classical/lectin pathway C3 convertase decay-accelerating activity as well as for factor I cofactor activity for C3b and C4b. Thus, CCPH is the first example wherein a single SCR domain has been shown to display complement regulatory functions