1,760 research outputs found
Improvement of Forage Quality by Means of Biotechnology: Stable Transformation of Warm-Season Grasses by Particle Bombardment
We have used a simple and inexpensive, self-built particle acceleration apparatus for direct delivery of DNA to cultured cells of warm-season grasses. High levels of transient expression of the b-glucuronidase gene were obtained following bombardment of embryogenic suspension cells and calli of bahiagrass (Paspalum notatum FlĆ¼gge) and dallisgrass (Paspalum dilatatum Poir). Furthermore, stable transformed calli of both species have been obtained using this simple particle gun
Transformation in Lotus Corniculatus: Towards Low-Lignin Pasture Through Antisense RNA
We have developed a rapid and reproducible transformation system for birdās-foot trefoil (Lotus corniculatus L.) by using Agrobacterium-mediated T-DNA transfer and the incorporation of the antisense gene for cinnamyl alcohol dehydrogenase (CAD) from Aralia cordata into Lotus for lignin reduction. The presence of the transferred antisense gene in regenerated plants has been confirmed by PCR analysis
Non-neutral processes drive the nucleotide composition of non-coding sequences in Drosophila
The nature of the forces affecting base composition is a key question in genome evolution. There is uncertainty as to whether differences in the GC contents of non-coding sequences reflect differences in mutational bias, or in the intensity of selection or biased gene conversion. We have used a polymorphism dataset for non-coding sequences on the X chromosome of Drosophila simulans to examine this question. The proportion of GCāAT versus ATāGC polymorphic mutations in a locus is correlated with its GC content. This implies the action of forces that favour GC over AT base pairs, which are apparently strongest in GC-rich sequences
Oxalate and silica contents of seven varieties of Napier grass (Pennisetum purpureum)
Oxalate and silica are considered antinutrients. Large quantities of oxalate and silica in plants can interfere with the uptake of essential minerals in ruminants. Therefore, the aim of this study was to compare the total silica and oxalate contents of seven varieties of Napier grass to find out which is best for cultivation. Taiwan, Zanzibar, Pakchong, Purple, Kobe, Indian, and Dwarf Napier grass were grown in a completely randomized design with three replications to determine their soluble oxalate, total oxalate, and silica contents. Plants were harvested at two months of plant maturity. Whole plant of the Dwarf Napier grass contained significantly higher soluble oxalate content than tall varieties. Total oxalate content in whole plant differed significantly among varieties. Dwarf showed the highest total oxalate content (3.23% dry matter (DM)) followed by Kobe (2.61%), Zanzibar (2.60%), Purple (2.44%), Taiwan (2.43%), Indian (2.15%), and Pakchong (1.95%). Regardless of variety, leaf tissue contained significantly higher soluble oxalate and total oxalate than stem tissue. There were no differences in silica content among them. In conclusion, the tall varieties could produce lower levels of soluble oxalate than the Dwarf variety, whereas silica content might not vary among them.Keywords: botanical fractions, mineral bioavailability, ruminan
High-temperature Superconductivity in Layered Nitrides \beta-LiMNCl (M = Ti, Zr, Hf): Insights from Density-functional Theory for Superconductors
We present an ab initio analysis with density functional theory for
superconductors (SCDFT) to understand the superconducting mechanism of doped
layered nitrides \beta-LiMNCl (M=Ti, Zr, and Hf). The current version of
SCDFT is based on the Migdal-Eliashberg theory and has been shown to reproduce
accurately experimental superconducting-transition temperatures Tc of a wide
range of phonon-mediated superconductors. In the present case, however, our
calculated Tc4.3 K (M=Zr) and 10.5 K (M=Hf) are found to be less
than a half of the experimental Tc. In addition, Tc obtained in the present
calculation increases with the doping concentration x, opposite to that
observed in the experiment. Our results indicate that we need to consider some
elements missing in the present SCDFT based on the Migdal-Eliashberg theory.Comment: 18 pages, 13 figures, submitted to Physical Review
Standard and generalized McDonaldāKreitman test: a website to detect selection by comparing different classes of DNA sites
The McDonald and Kreitman test (MKT) is one of the most powerful and extensively used tests to detect the signature of natural selection at the molecular level. Here, we present the standard and generalized MKT website, a novel website that allows performing MKTs not only for synonymous and nonsynonymous changes, as the test was initially described, but also for other classes of regions and/or several loci. The website has three different interfaces: (i) the standard MKT, where users can analyze several types of sites in a coding region, (ii) the advanced MKT, where users can compare two closely linked regions in the genome that can be either coding or noncoding, and (iii) the multi-locus MKT, where users can analyze many separate loci in a single multi-locus test. The website has already been used to show that selection efficiency is positively correlated with effective population size in the Drosophila genus and it has been applied to include estimates of selection in DPDB. This website is a timely resource, which will presumably be widely used by researchers in the field and will contribute to enlarge the catalogue of cases of adaptive evolution. It is available at http://mkt.uab.es
Universality of weak selection
Weak selection, which means a phenotype is slightly advantageous over
another, is an important limiting case in evolutionary biology. Recently it has
been introduced into evolutionary game theory. In evolutionary game dynamics,
the probability to be imitated or to reproduce depends on the performance in a
game. The influence of the game on the stochastic dynamics in finite
populations is governed by the intensity of selection. In many models of both
unstructured and structured populations, a key assumption allowing analytical
calculations is weak selection, which means that all individuals perform
approximately equally well. In the weak selection limit many different
microscopic evolutionary models have the same or similar properties. How
universal is weak selection for those microscopic evolutionary processes? We
answer this question by investigating the fixation probability and the average
fixation time not only up to linear, but also up to higher orders in selection
intensity. We find universal higher order expansions, which allow a rescaling
of the selection intensity. With this, we can identify specific models which
violate (linear) weak selection results, such as the one--third rule of
coordination games in finite but large populations.Comment: 12 pages, 3 figures, accepted for publication in Physical Review
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