Association of SSR markers with contents of fatty acids in olive oil and genetic diversity analysis of an olive core collection

The purpose of this research was to characterize an olive core collection using some agronomic characters and simple sequence repeat (SSR) markers and to determine SSR markers associated with the content of fatty acids in olive oil. SSR marker analysis demonstrated the presence of a high amount of genetic variation between the olive cultivars analyzed. A UPGMA dendrogram demonstrated that olive cultivars did not cluster on the basis of their geographic origin. Fatty acid components of olive oil in these cultivars were determined. The results also showed that there was a great amount of variation between the olive cultivars in terms of fatty acid composition. For example, oleic acid content ranged from 57.76 to 76.9% with standard deviation of 5.10%. Significant correlations between fatty acids of olive oil were observed. For instance, a very high negative correlation (-0.812) between oleic and linoleic acids was detected. A structured association analysis between the content of fatty acids in olive oil and SSR markers was performed. STRUCTURE analysis assigned olive cultivars to two gene pools (K = 2). Assignment of olive cultivars to these gene pools was not based on geographical origin. Association between fatty acid traits and SSR markers was evaluated using the general linear model of TASSEL. Significant associations were determined between five SSR markers and stearic, oleic, linoleic, and linolenic acids of olive oil. Very high associations (P < 0.001) between ssrOeUA-DCA14 and stearic acid and between GAPU71B and oleic acid indicated that these markers could be used for marker-assisted selection in olive

Identification of molecular markers associated with fruit traits in olive and assessment of olive core collection with AFLP markers and fruit traits

The purpose of this study was to characterize olive core collection with amplified fragment length polymorphism (AFLP) markers and fruit traits and to determine AFLP markers significantly associated with these fruit characters in olive. A total of 168 polymorphic AFLP markers generated by five primer combinations and nine fruit traits were used to characterize relationships between 18 olive cultivars. Although all olive cultivars were discriminated from each other by either AFLP markers (<0.75 similarity level) or fruit traits, clustering based on the AFLP markers and fruit traits was not significantly correlated (r = 0.13). Partial clustering of olive cultivars by AFLP markers according to their geographical origin was observed. Associations of AFLP markers with fruits were determined using a multiple-regression analysis with stepwise addition of AFLP markers. Significant associations between eight AFLP markers and fruit traits were identified. While five AFLP markers demonstrated significant negative correlation with fruit and stone weight, width and length and total polyphenols (P < 0.05), three AFLP markers displayed significant positive correlation with a-tocopherol and.-tocopherol (P < 0.01). This is the first report on the association of molecular markers with fruit traits in olive. Molecular markers associated with morphological and agronomic traits could be utilized for the breeding of olive cultivars. However, the association power of these markers needs to be confirmed in larger populations, and highly correlated markers should then be converted to PCR-based DNA markers such as sequence-characterized amplified region markers for better utilization

KÖPRÜ SÖKÜM TEKNİKLERİNİN İMPLANT DESTEKLİ SABİT PROTEZLER VE ÇEVRE KEMİK ÜZERİNDEKİ STRES DAĞILIMINA ETKİSİ: SONLU ELEMAN ANALİZİ

Background & Aims: Implant retained restorations may be retrieved due to many reasons. However, implant retained restorations have many components which may be damaged during removal. The aim of this study is to observe stress concentrations in the surrounding bone, the implant and its components, during retrieval of a three-unit implant-supported fixed partial denture using two different removal techniques. Materials and Methods: One three-dimensional digital model of an implant-supported three-unit restoration was constructed. The implants’ dimensions were 3.7 mm x 10mm. A pull out force of 40 N was applied on a single support and on the connectors with a loop device. The stress values were calculated within the dental implant, abutment, abutment screw and surrounding bone. Results: The highest stress concentration was observed at the collar of the abutment during load on a single support (16.246 MPa). The stress concentrations at the cortical bone were lower than the stress concentration at implants, while the maximum stress concentration in bone structure was 1.175 MPa. The loop device technique was enabled to share the load through both implants and reduce the stress concentration levels (9.941 MPa). Conclusion: The pull-out force, did not show a great effect in bone structure. However, implant components were under higher stress and abutment screw was the weakest part of the system. During a crown removal, more attention is needed in order not to damage implant components rather than the bone.Amaç: İmplant destekli restorasyonlar çeşitli nedenlerle çıkarılabilir. Ancak implant destekli restorasyonların söküm sırasında zarar görebilecek birçok parçası mevcuttur. Bu çalışmanın amacı, iki farklı çıkarma tekniğini kullanarak, üç üyeli implant destekli bir restorasyonun çkarılması sırasında; çevre kemik, implant ve bileşenlerindeki stres yoğunluklarını gözlemlemektir. Gereç ve Yöntem: Üç üyeli implant destekli bir restorasyonun üç boyutlu dijital modeli oluşturuldu. İmplantların boyutları 3.7 mm x 10 mm idi. 40 N’lık bir çekme kuvveti tek bir destek üzerinden ve konnektörlerden geçen bir tel ile uygulandı. Dental implant, abutment, abutment vidası ve çevre kemik içindeki stres değerleri hesaplandı. Bulgular: Tek bir destek üzerine uygulanan yük sırasında en yüksek gerilme konsantrasyonu abutment boynunda görülmüştür (16.246 MPa). Kemik yapısındaki maksimum stres konsantrasyonu 1.175 MPa iken, kortikal kemikteki stres konsantrasyonları, implantlardaki stres konsantrasyonundan daha düşük olarak gözlendi. Abutment vidalarındaki stres konsantrasyon seviyeleri benzer ancak lokalizasyonları farklıdır. Tel ile her iki konnektör üzerinden kuvvet uygulanması, yükün her iki implant tarafından paylaşlmasına ve stres konsantrasyon seviyelerinin (9.941 MPa) azalmasına olanak sağlamaktadır. Sonuç: Kron çıkarılması sırasında uygulanan çekme kuvveti, kemik yapısında büyük bir etki göstermemektedir. Bununla birlikte, implant bileşenleri daha yüksek gerilme konsantrasyonlarına maruz kalmıştır ve abutment vidası sistemin en zayıf bileşeni olmuştur. Bir kron sökümü sırasında, kemik yerine implant bileşenlerine zarar vermemek için daha fazla dikkat gerekir

The efficacy of albendazole treatment in a patient with hydatid cyst disease of multiorgan involvement

Hydatid cyst disease, which is caused by echinococcusgranulosus still poses a serious problem in endemic areas.The disease frequently involves liver and lung. Otherorgan involvements are rare. In a 18-year old patient, bilaterallung, right ventricle of heart, liver and spleen involvementwere detected. With albendazole treatmentcyst in heart was completely disappeared, and liver cystwas found to be decreased in size. However, no declinein the number and size of cysts in lung was observed.Therefore, it was concluded that albendazole may not beeffective in pulmonary hydatid disease compared to otherorgans.Key words: Albendazole, hydatid cyst, multiorgan involvemen

Management of persistent coccydynia with transrectal manipulation: Results of a combined procedure

WOS: 000431533600030PubMed ID: 29234884We compared the results of manual therapy combined with steroid injection with single steroid injection in the treatment of persistent coccydynia. Combined therapy was performed in 21 patients (Group 1) and steroid injection in 23 patients (Group 2). We compared two groups and investigated the combined therapy group in details. Patients were classified according to the underlying cause, BMI, anatomic type of coccyx and duration of symptoms (<6 or 6 months). Mean age was 30.5 years at the time of procedures. Mean followup time was 27.8 months. VAS scores were decreased in both groups but combined therapy group had much more better results. Complete pain relief was achieved in 61.9% of patients in Group 1 whereas it was only 17.4% in Group 2. In 23.8% of Group 1, the VAS score was significantly decreased but the feeling of uncomfortability persisted. This was 73.9% in Group 2. We had no relapse in Group 1 but in Group 2 the relapse rate was 56.5%. Underlying cause, body mass index, anatomic type of coccyx and duration of symptoms had no effect on results. Manual therapy combined with steroid injection would be an alternative method in case of persistent coccydynia. It is a safe and easy option before surgical treatment

Abdominal Invasion of Alveolar Cysts

Alveolar echinococcosis is a rare parasiter disease which caused by a taenia named Echinococcus multilocularis. Altough it is an enfectious disease, it has as malign character. Liver is the most effected member on this illness. After liver; lungs, kidneys, bone and brain are respectively effected. With experimental studies it is shown that peritoneal metastasis like lesions should occur by peritoneal spreading. In this article we aimed to call attention to this enfectious but malign behavioral disease by presenting an abdominal hydaditosis patient who was diagnosed and treated in our clinic

Mean platelet volume is associated with disease severity in patients with obstructive sleep apnea syndrome

OBJECTIVE: Obstructive sleep apnea syndrome is associated with cardiovascular diseases and thromboembolic events. The mean platelet volume (MPV) is a predictor of cardiovascular thromboembolic events. The aim of the present study is to investigate the association between the MPV and disease severity in patients with obstructive sleep apnea syndrome. METHODS: We prospectively included 194 obstructive sleep apnea syndrome patients without cardiovascular disease (mean age 56.5±12.5 years) who were undergoing sleep tests. An overnight full laboratory polisomnography examination was conducted on each patient. The patients were divided into 3 groups according to the apnea-hypopnea index (AHI): (1) AHIlow group: 5≤AH

Abdominal Invasion of Alveolar Cysts

Alveolar ekinokokkozis, echinococcus multilocularis adı verilen tenya tarafından sebep olunan nadir bir paraziter hastalıktır. Hastalık bir enfeksiyoz hastalık olmasına rağmen, davranış olarak malign karakter gösterir. Bu hastalıkta en yaygın etkilenen organ karaciğerdir. Karaciğeri sırasıyla akciğer, böbrek, kemik ve beyin izler. Peritoneal yayılım yoluyla peritoneal metastaz benzeri lezyonlara sebep olabileceği deneysel çalışmalarda gösterilmiştir. Bu yazımızda kliniğimizde teşhis ve tedavi edilen abdominal alveolar hidatidozisli bir hastayı sunarak, bu malign davranışlı infeksiyoz hastalığa dikkat çekmeyi amaçladık.Alveolar echinococcosis is a rare parasiter disease which caused by a taenia named Echinococcus multilocularis. Altough it is an enfectious disease, it has as malign character. Liver is the most effected member on this illness. After liver; lungs, kidneys, bone and brain are respectively effected. With experimental studies it is shown that peritoneal metastasis like lesions should occur by peritoneal spreading. In this article we aimed to call attention to this enfectious but malign behavioral disease by presenting an abdominal hydaditosis patient who was diagnosed and treated in our clinic

The comparison of local tumor control after microwave ablation, surgical resection and combined treatment for colorectal liver metastases

Aim. We aimed to compare the local therapeutic efficiency of microwave ablation (MWA), surgical resection, and combined treatment, assess the outcomes, and identify predictive factors for local treatment response in colorectal liver metastases (CLMs). Methods. From March 2013 to September 2019, a total of 54 patients with 302 CLMs were enrolled in this retrospective study. Eleven patients (20.4%) were treated with MWA, 9 patients (16.7%) with surgery, and 34 patients (63%) with the combined method. Univariate and multivariate analyses were performed to investigate overall survival (OS) and hepatic progression-free survival (HPFS) using the Cox proportional hazard regression model. The logistic regression analysis was used to identify the predictive factors for the local treatment response. Results. Total treatment response was achieved in 46.3% (n=25) of the patients. Local tumor progression was seen in 7.4% (n=4) of the patients, and the rate of intrahepatic distal recurrence was 46.3% (n=25). There were no significant differences in HPFS and OS between the three groups (p=0.56 and 0.90, respectively). Younger age

The impact of hybrid capture-based comprehensive genomic profiling on treatment strategies in patients with solid tumors

Objective: The development of bioinformatics and comprehensive genomic profiling (CGP) has provided insights into the ap-plicability and functionality of the genomic alterations (GA). In this study, we evaluated the impact of CGP on the treatment plan and outcomes in a significant number of patients. Material and Methods: We carried out a retrospective case-control study on 164 adult patients with advanced solid tumors from 15 oncology centers in Türkiye. Results: In all cases, CGP was performed within 23.8 [standard deviation (SD)±32.1] months of initial diagnosis. Non-small cell lung carcinoma, breast cancer, unknown primary carcinoma, colorectal carcinoma, and sarcoma were among the most common tumor types, accounting for 61.5% of all cases. CGP was performed immediately after the diagnosis of advanced cancer in 13 patients (7.9%). In 158 patients (96.4%), at least one GA was found as per the CGP report. Also, in the reports, the average tumor mutational burden (TMB) and GAs were 7.3 (SD±8.7) mut/Mb and 3.5 (SD±2.0), respectively. According to CGP reports, 58 patients had 79 evidence-based drug suggestions for their particular tumor type, whereas 97 patients had 153 evidence-based drug suggestions for another tumor type. After the primary oncologist interpreted the CGP reports, significant changes were made to the treatment of 35 (21.3%) patients. Conclusion: We strongly believe that in the future, high-TMB or other tumor-agnostic biomarkers will become much more afford-able, and CGP will serve as one of the major decision-making tools for the treatment of patients along with pathological, radiological or lab-oratory tests