Histological Sequences of Long Bone Development in the New Zealand White Rabbits

Long bone development occurred through endochondral ossification. The current study investigated the main histological changes associated with the development of humerus from the New Zealand white rabbits. The upper limb or humerus during embryonic (12, 14, 15, 16, 18, 21, 24 and 27 days) and neonatal (3 and 7 days) development was processed for light microscopy. Three stages preceded endochondral ossification; limb bud (mesenchymal cells), cartilage primordia (mesenchymal condensation and differentiation into chondrocytes) and cartilage template (organization of chondrocytes into proliferative and hypertrophic zones) by 12, 14 and 15 days, respectively. At day 16, cartilage template elongated forming a cartilaginous diaphysis of the prospective humerus. At day 18, cartilaginous epiphyses were formed. Medullary cavity was established by day 21 with no signs of calcification in the bone collar. Primary ossification center appeared within the diaphysis at day 24 of embryonic development. Secondary ossification centers were seen within the epiphyses by day 3 of neonatal development. Two types of cartilage canals were investigated; short epiphyseal canals appeared from day 21 and long transphyseal canals appeared from day 27. At day 7 of neonatal development, the cartilage of the humerus was limited in two places; articular epiphyseal growth cartilage between articular surface and secondary ossification center, epiphyseal physeal growth cartilage between the secondary and primary ossification centers. The current study summarized the main histological stages of development of rabbit humerus. These results should be considered in molecular studies of endochondral bone formation

Clinical significance and treatment of striae gravidarum during pregnancy: a review article

Abdominal stretch marks found during pregnancy may be indicative of poor skin elasticity. One who does not have stretch marks may have better skin elasticity and may be less likely to tear perineal and vaginal tissue during vaginal delivery. Striae gravidarum (SG) is a common phenomenon of stretch marks observed during pregnancy that may be an indicator of poor skin elasticity. The type and amount of collagen in connective tissue are considered to determine the individual’s elastic index. The skin surface is made up of a complex network of crossing thin lines. The two-dimensional relationship between primary and secondary lines determines the skin texture and its grade of irregularity. SG is caused by changes in the structural connective tissue due to a hormonal elect on the alignment and reduced elastin and fibrillin in the dermis. Severity scoring of SG was observed using the numerical scoring system of Atwal. This score provides a rank based on observation of four areas in which SG is most commonly observed (abdomen, hips, buttocks, and breast)

Tamoxifen for treatment of abnormal uterine bleeding in etonorgstrel implant users: a randomized clinical trial

Background: The current study aims to compare the use of tamoxifen and oral contraceptive pills in women using implanon and complain with irregular uterine bleeding.Methods: Women attended family planning clinic using implanon presented by bleeding were invited to participate in the study. They were randomized into two groups: Group A: 100 women received Tamoxifen 10 mg twice daily for 10 days taken at the onset of an episode of bleeding or spotting episode. Group B: 100 women received Combined oral contraceptive pills (microcept) once daily for 21 days take at the onset of an episode of bleeding or spotting episode.Results: No difference regarding the baseline criteria of both groups. No difference between both groups regarding the duration of irregular bleeding in the implanon users (p=0.090). Additionally, the number of bleeding days and spotting in the last month was similar in both groups (p=0.554). The percentage of women who stopped bleeding during the period of treatment is 84% in the tamoxifen group and 92% in the COCs group, but the COCs needs longer treatment time, where the mean of days required to stop bleeding is 5.03±1.8 days in the tamoxifen group and 6.5±2.5 in the COCs group. Headache and nausea were the most prominent adverse effects found in the COCs group (p=0.000).Conclusions: Oral administration of tamoxifen 10 mg twice daily for 10 days is effective on stopping bleeding attacks in implanon users

Promising anti-microbial effect of apple vinegar as a natural decolonizing agent in healthcare workers

Introduction: Colonized Healthcare workers (HCWs) are an essential reservoir of nosocomial infections. This study aims to determine the prevalence of methicillin-resistant Staphylococcus aureus (MRSA) carriage rate among HCWs, to evaluate at Fayoum University Hospital the susceptibility of isolates to mupirocin and Chlorhexidine and to investigate the antimicrobial effect of different vinegars on MRSA as a natural decolonizing agent. Methods: Nasal and hand swabs were collected from 124 HCWs at Fayoum University Surgical Hospital. Isolates were identified using the standard microbiological methods. Susceptibilities to mupirocin and Chlorhexidine were determined by disk diffusion and broth micro-dilution. Screening antimicrobial effect of commercial vinegars was determined by agar well-diffusion method and microdilution method. Results: About one tenth 11.3% (14/124) of HCWs showed nasal carriage of MRSA. Workers were the predominant carriers (P = 0.013). The overall non-nasal carriage rate of MRSA was 6.5% (8/124). Among MRSA isolates Low-level Mupirocin resistance (LLMR) showed in (36.4%, 8/22). MICs ranged from 0.25 to 32 μg/ml. Also, (13.6 %, 3/22) showed Chlorhexidine resistance, MICs ranged from 0.039 to 5 μg/ml. Apple vinegar showed the highest susceptibility among vinegars (p < 0.0001) with MIC values varied from 0.058 to 1.87 μg/ml Discussion: The emergence of mupirocin (36.4%) and Chlorhexidine (13.6%) resistant Staphylococcus aureus among HCWs should be of excessive concern. Apple vinegar has a promising antimicrobial effect against MRSA isolates and could be used as a decolonizing agent

Effects of Thiamethoxam on Vespula Germanica (F.) (Hymenoptera: Vespidae)

Damage of pesticides used in agriculture on non-target organisms (except honey bees) is not sufficiently considered and neglected. Studies showed that wild bees, like honey bees, were also affected by pesticides. Wild bee species should also be protected because of their importance in pollination and biological control and also in order to protect biodiversity. Effects of pesticides on wild bees should be investigated without delay and precautions should be taken to protect the generations of wildlife. In addition, while increasing the agricultural areas, habitat for wild pollinators should be established.In this study, effect of Thiamethoxam, an agriculturally active agent in the neonicotinoid group, commonly used in agriculture fields, on the European wasp (Vespula germanica) was investigated. For this purpose, 2 molar carnation-flavored syrup in a petri dish was placed in a field where wild bees are frequent, and wild bees had got accustomed there. Trial doses were prepared with thiamethoxam dose commonly used in agricultural areas (15 ml/100 L water) and 50% dilutions of this dose (15.00, 7.50, 3.75, 1.87, 0.93, 0.46, 0.23, 0.12, 0.06 ml/100 L water) and fed in 2 molar syrup. Those who returned to the carnation petri dish after feeding were recorded at the 1st, 4th and 24th hours.At the end of the study, bees fed with 15.00, 7.50, and 3.75 ml / 100 L doses of the pesticide all died. One hour after pesticide ingestion, 84.73% of the control group and 13.33% of the bees fed with 1.87 ml / 100L dose, were alive and returned to the syrup petri dish

A review on phytochemical, pharmacological and ethnopharmacological aspects of genus Trichodesma

The genus Trichodesma belongs to the family Boraginaceae. The plants of this genus are widely distributed in tropical and subtropical regions of Africa, Asia, and Australia. Phytochemically, scientific reports on Trichodesma species so far revealed more than one hundred compounds from this genus, including hydrocarbons, phenols, flavonoids, sterols, terpenes and alkaloids have been isolated or identified. Medicinally, various reported biological activities of Trichodesma such as antimicrobial, antiparasitic, cytotoxic, anti-infection, antioxidant, anti-inflammatory, anti-irritant, antidiarrheal, antispasmodic, antimalarial, analgesic, antipyretic, anti-diabetic, diuretic, and hepatoprotective effects were discussed in this review otherwise. Also, the ethnopharmacological effects of this genus were reviewed. Among all the Trichodesma species, T. indicum is regarded as the most important one regarding its pharmacological values. The volatile oil of T. africanum L. showed high antioxidant activity

AMELIORATIVE EFFECT OF CAROB AQUEOUS EXTRACT ON WATER PIPE SMOKE INDUCED-TOXICITY IN ADULT MALE ALBINO RATS

Objective: The aim of the present study was to investigate the daily effect of pre and post treatment of carob aqueous extract (600 mg/kg b. wt.); on rats exposed to were exposed to water pipe smoke (10 mg/kg; 15 minutes) for 2, 4, 6 and 8 w.Methods: Rats were divided into 6 groups; 1st group was served as a control. Rats of the 2nd group were administered amiodarone (an antiarrhythmic medication used as a model for lung toxicity) at a dose level of 30 mg/kg. The rats of 3rd and 4th groups were exposed to water pipe smoke and treated with carob extract respectively. However, the two remaining groups (5th and 6th) were the pre-and post-treatment groups with carob extract respectively.Results: Amiodarone and water pipe smoke induced a significant increase in serum alanine aminotransferase (ALT), aspartate aminotransferase (AST) and alkaline phosphatase (ALP); on the contrary, induced a significant decrease in serum total protein and albumin. In addition; serum creatinine and urea showed a significant increase; as well as; amiodarone and water pipe smoke increased the oxidative stress (malondialdehyde/glutathione; MDA/GSH) in lung tissue homogenate. While; carob extract gavage showed protective and curative effects in liver and kidney functions; in addition; it decreased MDA level and increased GSH level significantly.Conclusion: Finally, we can conclude that the carob aqueous extract treatment revealed ameliorative effects on rats were exposed to water pipe smoke

Moringa Oleifera Leaves in Broiler Diets: Effect on Chicken Performance and Health

Moringa products have a wide range of applications in agricultural, industrial and pharmaceutical processes. Moringa leaves have a relatively high crude protein content which varies from 25% to 32%. A high proportion of this protein is potentially available for digestion due to a high proportion of pepsin soluble nitrogen (82-91 %) and low proportion (1-2%) of acid detergent insoluble protein.Determination of chemical composition was evaluated in Moringa Leaves then Five iso-nitrogenous and iso-caloric experimental broiler diets were formulated as MOL0%, MOL5%, MOL10%, MOL15% and MOL20%, respectively and supplemented to broilers (10 chicks in each concentration) for 42 day. After 42nd day, chemical analysis of lipid profile(triglycerides, total cholesterol, HDL, LDL and vLDL), and haematological analysis(Hb, RBC, PCV, MCV, MCHC, Plt, MPV, PCT, PDW, WBC, LYM, MON, GRA) were recorded. Also tissue sampling from Bursa, Spleen, and Thymus were collected and preserved in 10 % formalin for histopathological examination. The obtained values were statistically analysed by one way analysis of variance (ANOVA) The chemical composition was determined in Moringa oleifera leaves, were ash, crude fiber, crude lipids, crude protein, total sugars, reducing sugar and non-reducing sugars. The highest effect of supplementation of moringa oleifera poultry diets on body weight of broiler, were 2293, 2318 and 2391gm, of treatments (10, 15 and 20% of MOL), respectively. Also, the more effective treatment were 20% of MOL of blood biochemical, lipid profile (triglycerides, total cholesterol, HDL, LDL and vLDL) and haematological parameters (HB, RBCs, Plt and WBCs), comparing with normal diets, histopathology of Bursa, Thymus, and Spleen showed improvement and hyperactivity in 15% and 20% MOL. Therefore, it is recommended to add Moringa oleifera at 15% and 20% in broiler diets to improve performance and health. Keywords: Broiler, Diets, Performance, Moring