Knowledge and Attitude of Saudi Arabian School Teachers with Regards to Emergency Management of Dental Trauma

Injury to both the primary and the permanent dentitions and their supporting structures is one of the most common dental problems seen in children.  School is one of the locations with greatest prevalence of occurrence of traumatic dental injuries.  School teachers play important role in prevention of dental trauma and improving its prognosis. The present study was undertaken to assess knowledge and attitudes regarding emergency management of dental trauma, first aid for avulsed tooth and influence of dental education among school teachers in Abha city. METHODS: The participants were 100 school teachers from Abha city and were interviewed using a questionnaire regarding management of dental trauma.  The completed proformas was collected and the result was statistically analyzed to know the knowledge and attitudes regarding emergency management of dental trauma. CONCLUSIONS: Majority of school staff had little knowledge related to handling of traumatic dental injuries and emergency management of avulsed permanent teeth in school children. Majority of school teachers were eager to have knowledge regarding dental trauma through continues dental education programs and workshops.  First aid training increases knowledge about dental trauma and should be included in the course curriculum of teachers training program

Erratum to: 36th International Symposium on Intensive Care and Emergency Medicine

[This corrects the article DOI: 10.1186/s13054-016-1208-6.]

Overexpression of <i>AtWRKY30</i> Transcription Factor Enhances Heat and Drought Stress Tolerance in Wheat (<i>Triticum aestivum</i> L.)

Drought and heat factors have negative impacts on wheat yield and growth worldwide. Improving wheat tolerance to heat and drought stress is of the utmost importance to maintain crop yield. WRKY transcription factors help improve plant resistance to environmental factors. In this investigation, Arabidopsis WRKY30 (AtWRKY30) transcription factor was cloned and expressed in wheat. Plants growth, biomass, gas-exchange attributes, chlorophyll content, relative water content, prolines content, soluble proteins content, soluble sugars content, and antioxidant enzymes activities (catalase (CAT), superoxide dismutase (SOD), peroxidase (POX), and ascorbate peroxidase (APX)) of the AtWRKY30-overexpressing wheat plants were higher than those of the wild type. However, levels of electrolyte leakage, malondialdehyde, and hydrogen peroxide of the AtWRKY30-overexpressing wheat plants were significantly less than those of the wild-type. Additionally, the expression level of antioxidant enzyme-encoding genes and stress-responsive genes (ERF5a, DREB1, DREB3, WRKY19, TIP2, and AQP7) were significantly induced in the transgenic wheat plants in comparison with the wild type. In conclusion, the results demonstrated that AtWRKY30 overexpression promotes heat and drought tolerance in wheat by inducing gas-exchange attributes, antioxidant machinery, osmolytes biosynthesis, and stress-related gene expression. AtWRKY30 could serve as a potential candidate gene for improving stress tolerance in wheat

A Framework for Quantifying the Strength of Partnerships between Agricultural Cooperatives and Development Actors: A Case Study in Saudi Arabia

The issue of the agricultural cooperatives&rsquo; sustainability in developing their businesses is gaining increasing prominence. Building partnerships between development actors and agricultural cooperatives has been considered an effective strategy for supporting financial capital and addressing sustainability issues collectively. Therefore, this study aimed to address the features and strengths of 33 partnerships established between the Beekeeping Cooperative Association in Al-Baha, Saudi Arabia, and other actors between 2016 and 2021. The analysis of the collaborations was based on six criteria: motivations, partnership planning, outputs, governance practices, outcomes, and sustainability of a partnership. Furthermore, we developed a weighted scoring model to control variable selection and submit the strength of each partnership. The findings indicated that most collaborations (45.5%) were signed with the private sector. Furthermore, the honey value chain development was the most frequent reason (69.7%) attracting the partners to engage in the partnerships. Some of the most critical environmental objectives targeted by the partnerships examined were enhancing bee habitat by the diversification of pasture species, management to increase the flowering period, and proper grazing management. All partners achieved their individual goals jointly in 54.5% of the partnerships analyzed. In terms of a partnership&rsquo;s strength, the findings also revealed that only three partnerships (9.1%) were characterized as strong partnerships. This study provides a better understanding of how agricultural cooperatives collaborate with other actors and a basis for assessing the strength of the partnerships. Such information is crucial for developing relevant policies to encourage cooperatives to engage in future sustainability partnerships

Analysis of Genetic Variation and Enhancement of Salt Tolerance in French Pea (Pisum Sativum L.)

Pisum sativum L. (field pea) is a crop of a high nutritional value and seed oil content. The characterization of pea germplasm is important to improve yield and quality. This study aimed at using fatty acid profiling and amplified fragment length polymorphism (AFLP) markers to evaluate the variation and relationships of 25 accessions of French pea. It also aimed to conduct a marker-trait associations analysis using the crude oil content as the target trait for this analysis, and to investigate whether 5-aminolevulinic acid (ALA) could enhance salt tolerance in the pea germplasm. The percentage of crude oil of the 25 pea genotypes varied from 2.6 to 3.5%, with a mean of 3.04%. Major fatty acids in all of the accessions were linoleic acid. Moreover, the 12 AFLP markers used were polymorphic. The cluster analysis based on fatty acids data or AFLP data divided the 25 pea germplasm into two main clusters. The gene diversity of the AFLP markers varied from 0.21 to 0.58, with a mean of 0.41. Polymorphic information content (PIC) of pea germplasm varied from 0.184 to 0.416 with a mean of 0.321, and their expected heterozygosity (He) varied from 0.212 to 0.477 with a mean of 0.362. The AFLP results revealed that the Nain Ordinaire cultivar has the highest level of genetic variability, whereas Elatius 3 has the lowest level. Three AFLP markers (E-AAC/M-CAA, E-AAC/M-CAC, and E-ACA/M-CAG) were significantly associated with the crude oil content trait. The response of the Nain Ordinaire and Elatius 3 cultivars to high salinity stress was studied. High salinity (150 mM NaCl) slightly reduced the photosynthetic pigments contents in Nain Ordinaire leaves at a non-significant level, however, the pigments contents in the Elatius 3 leaves were significantly reduced by high salinity. Antioxidant enzymes (APX&mdash;ascorbate peroxidase; CAT&mdash;catalase; and POD&mdash;peroxidase) activities were significantly induced in the Nain Ordinaire cultivar, but non-significantly induced in Elatius 3 by high salinity. Priming the salt-stressed Nain Ordinaire and Elatius 3 plants with ALA significantly enhanced the pigments biosynthesis, antioxidant enzymes activities, and stress-related genes expression, as compared to the plants stressed with salt alone. In conclusion, this study is amongst the first investigations that conducted marker-trait associations in pea, and revealed a sort of correlation between the diversity level and salt tolerance

Antagonistic Effect of Gut Bacteria in the Hybrid Carniolan Honey Bee, Apis Mellifera Carnica, Against Ascosphaera Apis, the Causal Organism of Chalkbrood Disease

The objective of this study was to isolate and characterize bacterial strains associated with the gut of the hybrid Carniolan honey bee, Apis mellifera carnica, and to determine their in vitro and in vivo potential against Ascosphaera apis, the causal organism of chalkbrood disease, with the purpose of exploring feasible biological control. Six bacterial strains were isolated from healthy worker honey bees by culture-dependent methods. Six fungal strains (A3, A4, A7, A8, A9, and A15) of A. apis were isolated from larvae suffering from chalkbrood disease on Yeast-Glucose-Starch agar (YGPSA) medium. All bacteria were identified by a combination of morphology, Gram stain, and 16S rRNA sequence analysis, and fungal strains were identified by morphology and 5.8S rRNA. In vitro and in vivo inhibition assays were carried out to determine the ability of bacterial isolates to inhibit A. apis, the causal agent of chalkbrood disease. The analysis of 16S rRNA sequences revealed that four bacterial strains (B2, B4, B10, and B100) belong to Bacillus subtilis species, and two strains (P1 and P5) belong to Pseudomonas fluorescence. Significant differences in antagonistic activity of all bacterial strains were observed. B. subtilis isolate B2 showed the highest antagonistic activity, as measured by the inhibition zone against A. apis, followed by the P1 strain of P. fluorescence. SEM analysis also supports the antagonistic activity of these bacteria against A. apis. This study provides a theoretical basis for biological control of honey bee chalkbrood disease