Mapping of fish spawning in the North Sea — Report of the KINO-2 project for 2018

Increased and updated knowledge of reproductive strategies of fish is crucial to optimize the temporal and spatial planning for conducting seismic surveys; in order to reduce its potential negative ecosystems impacts. The overarching goal of this study is to investigate the current spawning times and locations for the North Sea fish stocks. Molecular barcode and traditional visual taxonomic analyses were performed on eggs and larvae, systematically collected with plankton nets along a south to north axis in the North Sea. This report describes the establishments of methodology employed and preliminary results based on samples collected during August 2017 to September 2018. In total, 129 samples have been analysed from which 22 different fish taxa are identified. Based on historic data and published literature, our results agree with the expected number of the most prevalent fish species breeding in the North Sea. Both the taxonomic and molecular methods used, demonstrate similar results in the identification of taxonomic groups. The results further demonstrate that the molecular taxonomic approach is more appropriate for identification to species levels for some taxonomic groups, whilst the identification of egg and larvae developmental stages can only be performed using the visual taxonomic method. The two techniques complement each other to provide a detailed description of the annual fish spawning cycle for the main North Sea species. There are already emerging patterns evident in the results on the timing for breeding in some species at certain areas. However, improvements of the sampling methodology are still required, and an increased number of samples collected systematically over an extended time-period must be accomplished in order to provide appropriate data as basis for advice in timing of seismic surveys.publishedVersio

Mortality from gastrointestinal congenital anomalies at 264 hospitals in 74 low-income, middle-income, and high-income countries: a multicentre, international, prospective cohort study

Summary Background Congenital anomalies are the fifth leading cause of mortality in children younger than 5 years globally. Many gastrointestinal congenital anomalies are fatal without timely access to neonatal surgical care, but few studies have been done on these conditions in low-income and middle-income countries (LMICs). We compared outcomes of the seven most common gastrointestinal congenital anomalies in low-income, middle-income, and high-income countries globally, and identified factors associated with mortality. Methods We did a multicentre, international prospective cohort study of patients younger than 16 years, presenting to hospital for the first time with oesophageal atresia, congenital diaphragmatic hernia, intestinal atresia, gastroschisis, exomphalos, anorectal malformation, and Hirschsprung’s disease. Recruitment was of consecutive patients for a minimum of 1 month between October, 2018, and April, 2019. We collected data on patient demographics, clinical status, interventions, and outcomes using the REDCap platform. Patients were followed up for 30 days after primary intervention, or 30 days after admission if they did not receive an intervention. The primary outcome was all-cause, in-hospital mortality for all conditions combined and each condition individually, stratified by country income status. We did a complete case analysis. Findings We included 3849 patients with 3975 study conditions (560 with oesophageal atresia, 448 with congenital diaphragmatic hernia, 681 with intestinal atresia, 453 with gastroschisis, 325 with exomphalos, 991 with anorectal malformation, and 517 with Hirschsprung’s disease) from 264 hospitals (89 in high-income countries, 166 in middleincome countries, and nine in low-income countries) in 74 countries. Of the 3849 patients, 2231 (58·0%) were male. Median gestational age at birth was 38 weeks (IQR 36–39) and median bodyweight at presentation was 2·8 kg (2·3–3·3). Mortality among all patients was 37 (39·8%) of 93 in low-income countries, 583 (20·4%) of 2860 in middle-income countries, and 50 (5·6%) of 896 in high-income countries (p<0·0001 between all country income groups). Gastroschisis had the greatest difference in mortality between country income strata (nine [90·0%] of ten in lowincome countries, 97 [31·9%] of 304 in middle-income countries, and two [1·4%] of 139 in high-income countries; p≤0·0001 between all country income groups). Factors significantly associated with higher mortality for all patients combined included country income status (low-income vs high-income countries, risk ratio 2·78 [95% CI 1·88–4·11], p<0·0001; middle-income vs high-income countries, 2·11 [1·59–2·79], p<0·0001), sepsis at presentation (1·20 [1·04–1·40], p=0·016), higher American Society of Anesthesiologists (ASA) score at primary intervention (ASA 4–5 vs ASA 1–2, 1·82 [1·40–2·35], p<0·0001; ASA 3 vs ASA 1–2, 1·58, [1·30–1·92], p<0·0001]), surgical safety checklist not used (1·39 [1·02–1·90], p=0·035), and ventilation or parenteral nutrition unavailable when needed (ventilation 1·96, [1·41–2·71], p=0·0001; parenteral nutrition 1·35, [1·05–1·74], p=0·018). Administration of parenteral nutrition (0·61, [0·47–0·79], p=0·0002) and use of a peripherally inserted central catheter (0·65 [0·50–0·86], p=0·0024) or percutaneous central line (0·69 [0·48–1·00], p=0·049) were associated with lower mortality. Interpretation Unacceptable differences in mortality exist for gastrointestinal congenital anomalies between lowincome, middle-income, and high-income countries. Improving access to quality neonatal surgical care in LMICs will be vital to achieve Sustainable Development Goal 3.2 of ending preventable deaths in neonates and children younger than 5 years by 2030

Seasonal variability of metazooplankton in coastal sub-Antarctic waters (Beagle Channel)

The aim of this study was to analyse the variability in the species composition and abundance of the metazooplankton community in different coastal areas of the Beagle Channel (southern tip of South America) during a seasonal cycle. Sampling was conducted during November (spring) 2005, March (summer), June (autumn) and September (winter) 2006 at 12 coastal stations. Copepods were the most abundant group throughout the study, and their assemblages were composed of a mixture of species typical of the south-western Atlantic, the south-eastern Pacific and the Southern Ocean. Among them, Oithona similis, Ctenocalanus citer and Drepanopus forcipatus were the dominant species. The copepod Acartia tonsa was the only taxon that displayed a spatial pattern of abundance, showing higher densities in areas with lower salinities. The community structure showed a strong temporal pattern. The metazooplankton community in March and June was mainly composed of copepods, while in November and September the community showed a greater diversity. In these two months high densities of meroplanktonic larvae were found, in coincidence with higher chlorophyll-a concentration. This temporal pattern seems to be more dependent on primary production than on physical factors such as temperature or salinity. The absence of a clear spatial pattern may suggest that the studied area of the Beagle Channel behaves as a semi-enclosed water body.Fil: Aguirre, Gaston Ezequiel. Alfred Wegener Institute for Polar and Marine Research; Alemania. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Capitanio, Fabiana Lia. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Ecología, Genética y Evolución; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Lovrich, Gustavo Alejandro. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Austral de Investigaciones Científicas; ArgentinaFil: Esnal, Graciela Beatriz. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Ecología, Genética y Evolución; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentin

Mapping of fish spawning in the North Sea — Report of the KINO-2 project for 2018

Increased and updated knowledge of reproductive strategies of fish is crucial to optimize the temporal and spatial planning for conducting seismic surveys; in order to reduce its potential negative ecosystems impacts. The overarching goal of this study is to investigate the current spawning times and locations for the North Sea fish stocks. Molecular barcode and traditional visual taxonomic analyses were performed on eggs and larvae, systematically collected with plankton nets along a south to north axis in the North Sea. This report describes the establishments of methodology employed and preliminary results based on samples collected during August 2017 to September 2018. In total, 129 samples have been analysed from which 22 different fish taxa are identified. Based on historic data and published literature, our results agree with the expected number of the most prevalent fish species breeding in the North Sea. Both the taxonomic and molecular methods used, demonstrate similar results in the identification of taxonomic groups. The results further demonstrate that the molecular taxonomic approach is more appropriate for identification to species levels for some taxonomic groups, whilst the identification of egg and larvae developmental stages can only be performed using the visual taxonomic method. The two techniques complement each other to provide a detailed description of the annual fish spawning cycle for the main North Sea species. There are already emerging patterns evident in the results on the timing for breeding in some species at certain areas. However, improvements of the sampling methodology are still required, and an increased number of samples collected systematically over an extended time-period must be accomplished in order to provide appropriate data as basis for advice in timing of seismic surveys