Modulating vesicle adhesion by electric fields

We introduce an experimental setup for modulating adhesion of giant unilamellar vesicles to a planar substrate. Adhesion is induced by the application of an external potential to a transparent indium tin oxide-coated electrode (the substrate), which enables single-vesicle studies. We demonstrate tunable and reversible adhesion of negatively charged vesicles. The adhesion energy at different potentials is calculated from the vesicle shape assessed with confocal microscopy. Two approaches for these estimates are employed: one based on the whole contour of the vesicle and a second based on the contact curvature of the membrane in the vicinity of the substrate. Both approaches agree well with each other and show that the adhering vesicles are in the weak adhesion regime for the range of explored external potentials. Using fluorescence quenching assays, we detect that, in the adhering membrane segment, only the outer bilayer leaflet of the vesicle is depleted of negatively charged fluorescent lipids, while the inner leaflet remains unaffected. We show that depletion of negatively charged lipids is consistent Poisson-Boltzmann theory, taking into account charge regulation from lipid mobility. Finally, we also show that lipid diffusion is not significantly affected in the adhering membrane segment. We believe that the approaches introduced here for modulating and assessing vesicle adhesion have many potential applications in the field of single-vesicle studies and research on membrane adhesion

The redshift and broad band spectral energy distribution of NRAO 150

Context. NRAO 150 is one of the brightest radio and mm AGN sources on the northern sky. It has been revealed as an interesting source where to study extreme relativistic jet phenomena. However, its cosmological distance has not been reported so far, because of its optical faintness produced by strong Galactic extinction. Aims. Aiming at measuring the redshift of NRAO 150, and hence to start making possible quantitative studies from the source. Methods. We have conducted spectroscopic and photometric observations of the source in the near-IR, as well as in the optical. Results. All such observations have been successful in detecting the source. The near-IR spectroscopic observations reveal strong H$\alpha$ and H$\beta$ emission lines from which the cosmological redshift of NRAO 150 ($z=1.517\pm0.002$) has been determined for the first time. We classify the source as a flat-spectrum radio-loud quasar, for which we estimate a large super-massive black-hole mass $\sim5\times 10^{9} \mathrm{M_\odot}$. After extinction correction, the new near-IR and optical data have revealed a high-luminosity continuum-emission excess in the optical (peaking at $\sim2000$\,\AA, rest frame) that we attribute to thermal emission from the accretion disk for which we estimate a high accretion rate, $\sim30$\,% of the Eddington limit. Conclusions. Comparison of these source properties, and its broad-band spectral-energy distribution, with those of Fermi blazars allow us to predict that NRAO 150 is among the most powerful blazars, and hence a high luminosity -although not detected yet- $\gamma$-ray emitter.Comment: 8 pages, 4 figure

The magnetic field structure in CTA 102 from high-resolution mm-VLBI observations during the flaring state in 2016-2017

CONTEXT: Investigating the magnetic field structure in the innermost regions of relativistic jets is fundamental to understanding the crucial physical processes giving rise to jet formation, as well as to their extraordinary radiation output up to γ-ray energies. AIMS: We study the magnetic field structure of the quasar CTA 102 with 3 and 7 mm VLBI polarimetric observations, reaching an unprecedented resolution (∼50 μas). We also investigate the variability and physical processes occurring in the source during the observing period, which coincides with a very active state of the source over the entire electromagnetic spectrum. METHODS: We perform the Faraday rotation analysis using 3 and 7 mm data and we compare the obtained rotation measure (RM) map with the polarization evolution in 7 mm VLBA images. We study the kinematics and variability at 7 mm and infer the physical parameters associated with variability. From the analysis of γ-ray and X-ray data, we compute a minimum Doppler factor value required to explain the observed high-energy emission. RESULTS: Faraday rotation analysis shows a gradient in RM with a maximum value of ∼6 × 104⁴ rad m⁻² and intrinsic electric vector position angles (EVPAs) oriented around the centroid of the core, suggesting the presence of large-scale helical magnetic fields. Such a magnetic field structure is also visible in 7 mm images when a new superluminal component is crossing the core region. The 7 mm EVPA orientation is different when the component is exiting the core or crossing a stationary feature at ∼0.1 mas. The interaction between the superluminal component and a recollimation shock at ∼0.1 mas could have triggered the multi-wavelength flares. The variability Doppler factor associated with such an interaction is large enough to explain the high-energy emission and the remarkable optical flare occurred very close in time.Accepted manuscrip

Spatially resolved origin of mm-wave linear polarization in the nuclear region of 3C 84

We report results from a deep polarization imaging of the nearby radio galaxy 3C 84 (NGC 1275). The source was observed with the Global Millimeter VLBI Array (GMVA) at 86 GHz at an ultra-high angular resolution of 50μas (corresponding to 250R). We also add complementary multi-wavelength data from the Very Long Baseline Array (VLBA; 15 & 43 GHz) and from the Atacama Large Millimeter/submillimeter Array (ALMA; 97.5, 233.0, and 343.5 GHz). At 86 GHz, we measure a fractional linear polarization of ~ 2% in the VLBI core region. The polarization morphology suggests that the emission is associated with an underlying limb-brightened jet. The fractional linear polarization is lower at 43 and 15 GHz (~ 0.3-0.7% and < 0.1%, respectively). This suggests an increasing linear polarization degree towards shorter wavelengths on VLBI scales. We also obtain a large rotation measure (RM) of ~ 10⁵⁻⁶ rad/m² in the core at ≳43 GHz. Moreover, the VLBA 43 GHz observations show a variable RM in the VLBI core region during a small flare in 2015. Faraday depolarization and Faraday conversion in an inhomogeneous and mildly relativistic plasma could explain the observed linear polarization characteristics and the previously measured frequency dependence of the circular polarization. Our Faraday depolarization modeling suggests that the RM most likely originates from an external screen with a highly uniform RM distribution. To explain the large RM value, the uniform RM distribution, and the RM variability, we suggest that the Faraday rotation is caused by a boundary layer in a transversely stratified jet. Based on the RM and the synchrotron spectrum of the core, we provide an estimate for the magnetic field strength and the electron density of the jet plasma.Accepted manuscrip

La retracción en los morteros de cal

Nowadays, the methodology existing to measure the shrinkage in air, developed for paste and cement mortars, has serious problems to be applied to lime mortars, due to its different mechanism of hardening several modifications in Norms UNE 80-113-86 y 80-112-89 make possible the determination of the shrinkage in these traditional mortars.La metodología existente en la actualidad para la medida de la retracción de secado, desarrollada para las pastas y los morteros de cemento, presenta serios problemas a la hora de su aplicación a los morteros de cal debido a su distinto mecanismo de endurecimiento. Algunas modificaciones de las normas UNE 80-113-86 y 80-112-89 hacen posible la determinación de la retracción en estos morteros tradicionales

Resonant Kelvin-Helmholtz modes in sheared relativistic flows

Qualitatively new aspects of the (linear and non-linear) stability of sheared relativistic (slab) jets are analyzed. The linear problem has been solved for a wide range of jet models well inside the ultrarelativistic domain (flow Lorentz factors up to 20; specific internal energies $\approx 60c^2$). As a distinct feature of our work, we have combined the analytical linear approach with high-resolution relativistic hydrodynamical simulations, which has allowed us i) to identify, in the linear regime, resonant modes specific to the relativistic shear layer ii) to confirm the result of the linear analysis with numerical simulations and, iii) more interestingly, to follow the instability development through the non-linear regime. We find that very high-order reflection modes with dominant growth rates can modify the global, long-term stability of the relativistic flow. We discuss the dependence of these resonant modes on the jet flow Lorentz factor and specific internal energy, and on the shear layer thickness. The results could have potential applications in the field of extragalactic relativistic jets.Comment: Accepted for publication in Physical Review E. For better quality images, please check http://www.mpifr-bonn.mpg.de/staff/mperucho/Research.htm

Nosocomial transmission of multidrug-resistant Mycobacterium tuberculosis in Spain.

Before 1990, outbreaks of multidrug-resistanttuberculosis (MDRTB) were uncommon (1); sincethen, more than 10 outbreaks have been reported,all in hospitals and prisons in the eastern UnitedStates (2-7). Persons traditionally considered atrisk for MDRTB (foreign-born TB patients andthose inadequately treated for TB) have not beenassociated with these outbreaks. Instead, the pres-ence of patients with active TB near immunocom-promised patients in HIV-dedicated wards has ledto MDRTB-infected HIV patients whose TB casesoften go unrecognized. The patients receive inade-quate treatment in facilities without effective pro-cedures for isolating acid-fast bacilli; thesecircumstances favor nosocomial transmission.Health officials in other geographic areas whereHIV and TB are major public health threats havebeen alerted to this emerging problem, and sur-veillance systems have been designed (8).Drs. Herrera, Peiró, Castell, and Godoy have received ascholarship from the Fondo de Investigación Sanitaria delInstituto de Salud Carlos III during their 2-year epidemiologictraining period in the Field Epidemiology Training Program(Programa de Epidemiología Aplicada de Campo)

Sub-milliarcsecond imaging of a bright flare and ejection event in the extragalactic jet 3C 111

Flares in radio-loud AGN are thought to be associated with the injection of fresh plasma into the compact jet base. Such flares are usually strongest and appear earlier at shorter radio wavelengths. Hence, VLBI at mm-wavelengths is best suited to study the earliest structural changes of compact jets associated with emission flares. We study the morphological changes of the parsec-scale jet in the nearby (z=0.049) gamma-ray bright radio galaxy 3C111 following a flare that developed into a major radio outburst in 2007. We analyse three successive observations of 3C111 at 86 GHz with the Global mm-VLBI Array (GMVA) between 2007 and 2008 which yield a very high angular resolution of ~45muas. In addition, we make use of single-dish radio flux density measurements from the F-GAMMA and POLAMI programmes, archival single-dish and VLBI data. We resolve the flare into multiple plasma components with a distinct morphology resembling a bend in an otherwise remarkably straight jet. The flare-associated features move with apparent velocities of ~4.0c to ~4.5c and can be traced also at lower frequencies in later epochs. Near the base of the jet, we find two bright features with high brightness temperatures up to ~10^11K, which we associate with the core and a stationary feature in the jet. The flare led to multiple new jet components indicative of a dynamic modulation during the ejection. We interpret the bend-like feature as a direct result of the outburst which makes it possible to trace the transverse structure of the jet. In this scenario, the components follow different paths in the jet stream consistent with expectations for a spine-sheath structure, which is not seen during intermediate levels of activity. The possibility of coordinated multiwavelength observations during a future bright radio flare in 3C111 makes this source an excellent target for probing the radio-gamma-ray connection.Comment: 11 pages, 7 figures, accepted for publication by Astronomy & Astrophysic

Sub-milliarcsecond imaging of a bright flare and ejection event in the extragalactic jet 3C 111

ContextFlares in radio-loud active galactic nuclei are thought to be associated with the injection of fresh plasma into the compact jet base. Such flares are usually strongest and appear earlier at shorter radio wavelengths. Hence, very long baseline interferometry (VLBI) at millimeter(mm)-wavelengths is the best-suited technique for studying the earliest structural changes of compact jets associated with emission flares. AimsWe study the morphological changes of the parsec-scale jet in the nearby (z = 0.049) γ-ray bright radio galaxy 3C 111 following a flare that developed into a major radio outburst in 2007. Methods\ua0We analyse three successive observations of 3C 111 at 86 GHz with the Global mm-VLBI Array (GMVA) between 2007 and 2008 which yield a very high angular resolution of ∼45  μas. In addition, we make use of single-dish radio flux density measurements from the F-GAMMA and POLAMI programmes, archival single-dish and VLBI data. ResultsWe resolve the flare into multiple plasma components with a distinct morphology resembling a bend in an otherwise remarkably straight jet. The flare-associated features move with apparent velocities of ∼4.0c to ∼4.5c and can be traced also at lower frequencies in later epochs. Near the base of the jet, we find two bright features with high brightness temperatures up to ∼1011 K, which we associate with the core and a stationary feature in the jet. ConclusionsThe flare led to multiple new jet components indicative of a dynamic modulation during the ejection. We interpret the bend-like feature as a direct result of the outburst which makes it possible to trace the transverse structure of the jet. In this scenario, the components follow different paths in the jet stream consistent with expectations for a spine-sheath structure, which is not seen during intermediate levels of activity. The possibility of coordinated multiwavelength observations during a future bright radio flare in 3C 111 makes this source an excellent target for probing the radio-γ-ray connection