An Investigation upon DNA Interaction of 2 3 , 9 10 , 16 17 , 23 24 –Tetrakis 4- 4- 2-phenylprop-2-yl phenoxy Phthalocyanine Compound

The DNA binding activity of previously synthesized and characterized 2 3 , 9 10 , 16 17 , 23 24 –Tetrakis 4- 4- 2-phenylprop-2-yl phenoxy phthalocyanine compound PcF with CT-DNA was studied using UV/Vis, emission spectroscopic titrations, the melting temperature, viscosity measurement, and agarose gel electrophoresis methods in a Tris-HCl buffer solution at a pH of 7.1 at room temperature. The absorption titration spectra studies of PcF showed that absorbance intensities were decreased with increasing of concentrations of CT-DNA and the finding proved that the compound interacts with the DNA. Addition to absorption titration study, emission spectroscopic titration, the melting temperature, viscosity measurement, and agarose gel electrophoresis methods were also performed to investigate the binding activities of PcF with the DNA. The results of these methods confirmed the findings of absorption spectra study that the compound interacts with the DNA

DNA-Binding Properties of Nickle II Phthalocyanine with 2-Isopropyl-5-Methylphenoxy Substituents and Their Studies of UV/Vis, Fluorescence Spectroscopies, Cyclic voltametry, Gel Eelectrophoresis and Viscosity Measurement

I n this study, previously synthesized Ni II phthalocyanine compound bearing 2,10,16,24-tetrakis 2-isopropyl-5-methylphenoxy group was chosen for its interaction with calf thymus-DNA. Calf thymus-DNA was used to determine DNA binding properties of Ni II phthalocyanine compound. The DNA binding activities of Ni II phthalocyanine compound bearing 2-isopropyl-5-methylphenoxy substituent was investigated by using absorption titration, fluorescence emission, cyclic voltammetry, gel elctrophoresis in Tris-HCl buffer at pH 7.0. In addition to above methods, melting point and viscosity experiment were performed to determine the DNA intercation of the compound in Tris-HCl buffer solution. The results showed that Ni II phthalocyanine compound binds strongly to calf thymus-DNA via intercalation bindin

Synthesis of phthalocyanines crosswise-substituted with two alkylsulfanyl and two crown-ether

1236-1238Crosswise-substutited phthalocyanines with two nitro and two dodecylsulfanyl groups are synthesized by a 1:1 condensation of 1,3-dihydro-1,3-diimino-6-(n-dodecylsulfanyl) isoimino indolenine with 6-nitro-1,3,3-trichloroisoindolenine in the presence of sodium methoxide, hydroquinone and triethylamine. Nitro substituents are reduced to amines by sodium sulfide and condensation of the amino groups with benzo-15-crown-5-aldehyde results in a new Pc with two benzo-15-crown-5-imino substituents. The compounds have been characterized by elemental analyses, UV/Vis and IR spectroscopy. </span

The study on biological activities of silver nanoparticles produced via green synthesis method using Salvia officinalis and Thymus vulgaris

In the present study, Ag nanoparticles (AgNPs) were synthesized from Salvia officinalis and Thymus vulgaris, known as phytotherapy plants. The obtained silver nanoparticles were characterized using SEM, XRD, FTIR, and UV/Vis spectra. The antioxidant capacities of Salvia officinalis-mediated AgNP (SO-AgNP) and Thymus vulgaris-mediated AgNP (TV-AgNP) were analyzed in vitro using 1,1-diphenyl-2-picrylhydrazyl and iron chelating activity assays. DPPH activities were 83.74% and 57.17% for SO-AgNP and TV-AgNP at concentration 200 mg/L, respectively. Both green synthesized AgNPs exhibited good iron chelating activity. In addition, the DNA cleavage activities of SO-AgNPs and TV-AgNP were investigated with agarose gel electrophoresis technique. SO-AgNPs and TV-AgNP showed single-strand DNA cleavage activity. AgNPs showed that the SO-AgNP and TV-AgNp were effective against bacteria and fungi, and antimicrobic activities were assessed as minimal inhibition concentration (MIC). Remarkably, green synthesized AgNPs showed highly effective cell viability and biofilm inhibition effect. AgNPs also demonstrated slightly antimicrobial photodynamic activity after LED irradiation

Co and Zn Metal Phthalocyanines with Bulky Substituents: Anticancer, Antibacterial Activities and Their Inhibitory Effects on Some Metabolic Enzymes with Molecular Docking Studies

WOS:000630387800001In this study, we reported the synthesis of new cobalt and zinc phthalocyanine compounds with ((ethylenediamine-N,N',N'-triacetic acid-N-2-ethyl)oxy) (ETAEO) substituted groups. Characterization studies and anticancer properties of both synthesized compounds were determined as well. The inhibitory effects of these complexes on some metabolic enzymes were examined and it was observed that the enzymes inhibited by complex molecules at the micromolar levels. In addition, the active sites of the enzymes were determined by molecular modeling programme for screening the enzyme-inhibitor interactions. The molecular docking method was used to calculate the interactions of molecules with enzymes. Also, human prostate and breast cancer cell lines (PC-3 and MCF-7) were used to determine the anticancer properties of the complexes. All doses of the tetrakis (ETAEO) phthalocyaninato Cobalt II (1) did not show any significant changes in PC-3 cell viability, but significantly reduced in MCF-7 cell viability. Similarly, all doses of the tetrakis (ETAEO) phthalocyaninato zinc II (2) significantly reduced MCF-7 cell viability compared to the control and solvent control groups. According to the enzyme inhibition studies, both complexes showed the best inhibition effects for alpha-Glycosidase enzyme with 125.85 +/- 30.35 mu M and 165.30 +/- 27.18 mu M Ki values