USING WIND TURBINES TO GENERATE HEAT

In this article discusses one of the alternative renewable energy sources – wind energy. Its practical use is carried out with the help of wind motors. And below are a few different schemes of heat generation using these engines.В данной статье рассматривается один из альтернативных возобновляемых источников энергии – энергия ветра. Её практическое использование осуществляется с помощью ветряных двигателей. Представлены несколько различных схем выработки тепла с помощью данных двигателей

Structural study of the type II 3-dehydroquinate dehydratase from Actinobacillus pleuropneumoniae

9 pages, 7 figures, 2 tables.The structure of the type II dehydroquinate dehydratase (DHQase) from Actinobacillus pleuropneumoniae, the third enzyme of the shikimate pathway, has been determined. Crystals diffracting to 1.7 Å were obtained in space and on earth using the counter-diffusion technique. The structure was solved using molecular replacement and refined to high resolution. The overall structure of the dodecameric enzyme is described and compared with structures of DHQases from other bacteria. DHQases contain a flexible loop that presumably closes over the active site upon substrate binding. The enzyme can exist in an open or closed conformation. The present structure displays the open conformation, with a sulfate anion bound in the active site. The availability of this structure opens a route to structure-based antibiotics targetting this pathogenic bacterium.We thank Professor Kabsch for providing XDS free of charge. We acknowledge the support of the European Space Agency and the European Community Action to Research Infrastructure Action of the Improving Human Potential Programme to the EMBL Hamburg Outstation, contract No. HPRI-CT-1999-00017. We thank Olivier Minster (ESA) for his support of space science. The authors acknowledge the excellent work of Dr Eva ManÄ as in managing the logistics concerning the space mission. We thank Viscount Dirk Frimout for his support for space crystallization experiments.Peer reviewe

Interaction of eukaryotic translation initiation factor 4G with the nuclear cap-binding complex provides a link between nuclear and cytoplasmic functions of the m7 guanosine cap

In eukaryotes the majority of mRNAs have an m7G cap that is added cotranscriptionally and that plays an important role in many aspects of mRNA metabolism. The nuclear cap-binding complex (CBC; consisting of CBP20 and CBP80) mediates the stimulatory functions of the cap in pre-mRNA splicing, 3' end formation, and U snRNA export. As little is known about how nuclear CBC mediates the effects of the cap in higher eukaryotes, we have characterized proteins that interact with CBC in HeLa cell nuclear extracts as potential mediators of its function. Using cross-linking and coimmunoprecipitation, we show that eukaryotic translation initiation factor 4G (eIF4G), in addition to its function in the cytoplasm, is a nuclear CBC-interacting protein. We demonstrate that eIF4G interacts with CBC in vitro and that, in addition to its cytoplasmic localization, there is a significant nuclear pool of eIF4G in mammalian cells in vivo. Immunoprecipitation experiments suggest that, in contrast to the cytoplasmic pool, much of the nuclear eIF4G is not associated with eIF4E (translation cap binding protein of eIF4F) but is associated with CBC. While eIF4G stably associates with spliceosomes in vitro and shows close association with spliceosomal snRNPs and splicing factors in vivo, depletion studies show that it does not participate directly in the splicing reaction. Taken together the data indicate that nuclear eIF4G may be recruited to pre-mRNAs via its interaction with CBC and accompanies the mRNA to the cytoplasm, facilitating the switching of CBC for eIF4F. This may provide a mechanism to couple nuclear and cytoplasmic functions of the mRNA cap structure

Самонастраивающаяся система автоматического управления посевным агрегатом

The selfadjusting automatic control of sowing unit and differentiated introduction of mineral fertilizers doses according to agrochemical indicators of the soil (precision agriculture) are used wider nowadays. It was defined that the main requirement to the differentiated seeding and fertilizing is an accuracy and duration of transition from one norm to another. Established that at a speed of unit of 10 km/h object moves for 0.5 s about on 1.5 m and more. Thus in this device the radio channel originated differentiated correction is updated in 10 s, and in the RTK mode - 0.5-2 s that breaks the accuracy of introduction of seeds and fertilizers. The block schematic diagram of system of automatic control of technological process of seeding and mineral fertilizing with use of navigation means of machine-tractor aggregates orientation in the field and technical means for realization of technology of precision agriculture at sowing and fertilizers application due to electronic maps of soil fertility and navigation satellite systems was worked out. It was noted that for regulation of a fertilizing dose it is necessary to complete the unit with the electric drive, and for error reduction use navigation GLONASS, GPS, Galileo receivers. To tracking of four leading navigation systems GPS/GLONASS/Galileo/Compass receiver with 32 canals developed by domestic-owned firm «KB NAVIS» was suggested. It was established that the automated device created by All-Russia Research Institute of Mechanization for Agriculture information based on NAVSTAR and GLONASS/GPS system successfully operates seeding and make possible the differentiate fertilizing.В настоящее время большое развитие получает применение автоматизации управления посевным агрегатом и дифференцированным внесением доз минеральных удобрений в соответствии с агрохимическими показателями почвы (точное земледелие). Определили, что главное требование к дифференцированным высеву семян и внесению минеральных удобрений - это точность и продолжительность перехода с одной нормы на другую. Установили, что при скорости агрегата 10 км/ч объект перемещается за 0,5 с на 1,5 м и более. При этом в выпускаемом устройстве дифференцированная поправка, поступающая по радиоканалу, обновляется через 10 с, а в режиме RTK - через 0,5-2 с, что нарушает точность внесения семян и удобрений. Разработали структурную схему системы автоматического регулирования технологического процесса высева семян и внесения минеральных удобрений с использованием навигационных средств ориентации МТА на поле и технические средства для реализации технологии точного земледелия при посеве и внесении удобрений с помощью электронных карт плодородия земель и спутниковых навигационных систем. Отметили, что для регулирования дозы внесения удобрений необходимо укомплектовать агрегат электроприводом, а для уменьшения погрешности - использовать навигационные приемники ГЛОНАСС, GPS, Galileo. Предложили для слежения четырех ведущих навигационных систем применять разработанный отечественной фирмой «КБ НАВИС» приемник GPS/ГЛОНАСС/Galileo/Compass на 32 канала. Установили, что созданное ВИМ информационное автоматизированное устройство на базе NAVSTAR и системы ГЛОНАСС/GPS успешно управляет посевом и позволяет вносить удобрения дифференцированн

Immunization with the Haemophilus ducreyi Hemoglobin Receptor HgbA with Adjuvant Monophosphoryl Lipid A Protects Swine from a Homologous but Not a Heterologous Challenge

Haemophilus ducreyi, the etiological agent of chancroid, has a strict requirement for heme, which it acquires from its only natural host, humans. Previously, we showed that a vaccine preparation containing the native hemoglobin receptor HgbA purified from H. ducreyi class I strain 35000HP (nHgbAI) and administered with Freund's adjuvant provided complete protection against a homologous challenge. In the current study, we investigated whether nHgbAI dispensed with monophosphoryl lipid A (MPL), an adjuvant approved for use in humans, offered protection against a challenge with H. ducreyi strain 35000HP expressing either class I or class II HgbA (35000HPhgbAI and 35000HPhgbAII, respectively). Pigs immunized with the nHgbAI/MPL vaccine were protected against a challenge from homologous H. ducreyi strain 35000HPhgbAI but not heterologous strain 35000HPhgbAII, as evidenced by the isolation of only strain 35000HPhgbAII from nHgbAI-immunized pigs. Furthermore, histological analysis of the lesions showed striking differences between mock-immunized and nHgbAI-immunized animals challenged with strains 35000HPhgbAI but not those challenged with strain 35000HPhgbAII. Mock-immunized pigs were not protected from a challenge by either strain. The enzyme-linked immunosorbent assay (ELISA) activity of the nHgbAI/MPL antiserum was lower than the activity of antiserum from animals immunized with the nHgbAI/Freund's vaccine; however, anti-nHgbAI from both studies bound whole cells of 35000HPhgbAI better than 35000HPhgbAII and partially blocked hemoglobin binding to nHgbAI. In conclusion, despite eliciting lower antibody ELISA activity than the nHgbAI/Freund's, the nHgbAI/MPL vaccine provided protection against a challenge with homologous but not heterologous H. ducreyi, suggesting that a bivalent HgbA vaccine may be needed

A Case of Medullary Carcinoma of the Jejunum Combined with the Intestinal Lymphangiectasia Accompanied by the Malabsorption Syndrome

Aim: to present a clinical and morphological observation of an extremely rare combination of medullary carcinoma of the jejunum and intestinal lymphangiectasia in a 33-year-old patient with clinical features of malabsorption syndrome over the 10 years.Key points. An autopsy revealed a tumor formation spreading from the wall of the jejunum to the mesentery, with metastases to the mesenteric lymph nodes. The medullary carcinoma with positive expression of СD117, DOG1, EMA, PanCK, PDL-1, vimentin, mosaic non-intense expression of CA19-9, calretinin, CD10, CDX2, CEA, MUC-5AC, SATB2, and negative reaction to ALK, CD3, CD8, CD20, CD30, CD31, CD34, CD45, CD56, chromogranin, CK7, CK20, desmin. The proliferative index was high: Ki-67 > 80 %. Moreover, during the histological examination of the intestinal wall, intestinal lymphangiectasia complicated by the malabsorption syndrome was revealed.Conclusion. The uniqueness of this clinical and morphological case is in the combination of medullary carcinoma of the jejunum metastasized to the mesenteric lymph nodes with the underlying intestinal lymphangiectasia accompanied by the development of malabsorption syndrome

Quantitative analysis of ribosome–mRNA complexes at different translation stages

Inhibition of primer extension by ribosome–mRNA complexes (toeprinting) is a proven and powerful technique for studying mechanisms of mRNA translation. Here we have assayed an advanced toeprinting approach that employs fluorescently labeled DNA primers, followed by capillary electrophoresis utilizing standard instruments for sequencing and fragment analysis. We demonstrate that this improved technique is not merely fast and cost-effective, but also brings the primer extension inhibition method up to the next level. The electrophoretic pattern of the primer extension reaction can be characterized with a precision unattainable by the common toeprint analysis utilizing radioactive isotopes. This method allows us to detect and quantify stable ribosomal complexes at all stages of translation, including initiation, elongation and termination, generated during the complete translation process in both the in vitro reconstituted translation system and the cell lysate. We also point out the unique advantages of this new methodology, including the ability to assay sites of the ribosomal complex assembly on several mRNA species in the same reaction mixture

Analysis of the dynamics and structure of morbidity of athletes of the national teams of Moscow based on the results of medical examination

Objective: The aim of the study was to analyze the structure and dynamics of morbidity of athletes, members of sports teams of the city of Moscow based on the results of an in-depth medical examination in the period 2017–2021.Materials and methods: The data of the medical examination of athletes, which took place at the Moscow scientific and Practical Center for Medical Rehabilitation, restorative and sports medicine, including instrumental, functional and laboratory studies from 2017 to 2021, were analyzed. In 2017, 15176 athletes were examined, in 2018 and 2019, 11200, in 2020, 10080 people and in 2021–11922 athletes.Results: The results of the study showed an increase in the proportion of amenorrhea within the triad of athletes, an increase in general therapeutic morbidity, diseases of the gastrointestinal tract, among which the proportion of secondary hyperbilirubinemia in 2021 increased 3 times compared to 2020, which, in our opinion, is also due to the impact of the pandemic and quarantine restrictions.The high prevalence of ophthalmological morbidity of athletes and changes in the cardiovascular system is shown. During 2017–2021, a change in the structure of cardiac morbidity was revealed in the form of an increase in the proportion of cardiac arrhythmias and hypertensive response to stress.An increase in the endocrine morbidity of athletes in 2021 was revealed due to an increase in the proportion of hypothyroidism and more frequent detection of hyperthyroidism, possibly related to the COVID-19 pandemic, which requires further research.There was also an increase in diseases of the skin and genitourinary system in athletes. Psychological problems of athletes increased during the observation period from 2017 to 2021. There was no significant dynamics of surgical and traumatological diseases, otolaryngological pathologу.Conclusion: The results of the study indicate the importance of regular full-fledged examination of athletes, identification of various functional disorders and diseases for their timely prevention and treatment in order to preserve the health of athletes and improve athletic performance