Effect of storage time on microbial quality of some spices and dried seasonings

The effect of storage time on the microbial quality of some spices and dried seasonings (SDS) (dawadawa, pepper, ginger, shrimp and fish powders) was studied over a 12-month period. Microbial load and profile of irradiated and unirradiated SDS were assessed at 0, 6 and 12-month periods. The range of total variable counts (TVCs) were initially determined at 0.81-4.53 and 4.65-8.51 log10 cfu g-1 for irradiated and unirradiated SDS, respectively; those for mould and yeast counts (MYCs) were determined at 0-1.74 and 1.55-3.35 log10 cfu g-1, respectively. Generally, TVCs were not significantly affected (

Irradiation of ready meals for microbiological safety and shelf-life extension. 1. Microbiological quality of waakye and other ready-to-eat meals

Waakye bought from the open market and 14 meals prepared under the hazard analysis and critical control point (HACCP) plan were microbiologically investigated. The aerobic mesophilic count (AMC) (107 - 108 CFU g-1) and coliform count (106 - 107 CFU g-1) ) for complete waakye meals, including macaroni, fried fish, sauce and vegetable salad, exceeded the microbiological standards for such ready-to-eat meals. The AMC (101 - 104 CFU g-1) ) and coliform count (101- 102 CFU g-1) ) for all the ready meals prepared under HACCP and stored (-5 to 0 oC) for up to 5 days were within the standards. Potential pathogens isolated from waakye and the meals prepared under HACCP plan included Escherichia coli, Klebsiella spp., Serratia spp., and Enterobacter spp. Subsequent studies will exploit the potential of irradiation to eliminate pathogens and ensure the microbiological safety of ready-to-eat mealsWaakye (riz et haricot cuisinés ensemble) acheté de la vente libre ainsi que quatorze repas préparés sous le critique (ARPCC) étaient enquetés par la méthode microbiologique. Le compte d'aerobic mésophilique (107 - 108 CFU g-1) et le compte coliforme (106 - 107 CFU g-1) ) pour le repas de Waakye complet qui comprenait le macaroni, le poisson frit, la sauce et la salade de légumes dépassaient les normes microbiologiqes pour tels repas cuisinés. Le compte d'aerobic mésophilique (101 - 104 CFU g-1) ) et le compte coliforme (101 - 102 CFU g-1) ) pour tous les plats cuisinés préparés sous ARPCC et conservés au froid (-5 à 0 oC) pour 5 jours étaient aux niveaux requis. Les agents pathogènes potentiels isolés de waakye et des repas préparés sous le plan d'ARPCC comprenaient Escherichia coli, Klebsiella spp., Serratia spp., and Enterobacter spp. Les études à venir vont exploiter l'utilisation d'irradiation pour éliminer les agents pathogènes et assurer la sécurité microbiologique des repas cuisinés. Ghana Journal of Agricultural Science Vol. 39 (1) 2006: pp. 19-2

State Fragility, Regime Survival and Spoilers in South Sudan

This paper draws on the notion of state fragility in three dimensions – Authority failures, Service delivery failures and Legitimacy failures as developed by Stewart and Brown. Using Stewart and Brown’s analysis of fragile states, the authors examine how recent events in South Sudan push the country into being the most fragile state. In furthering this three-dimensional approach, we attempt two important questions. How has South Sudan succumbed to fragility since attaining independence? Who influences peace in the country? The authors grapple with these questions by investigating events in South Sudan from the period of signing the Comprehensive Peace Agreement (CPA 1 2005), independence in 2011, signing CPA 2 in 2015 up to present. The paper singles out the desire for regime survival as the major cause of fragility. The authors further argue that insecurity and instability are exacerbated by spoiling behaviour of certain powers and individuals, whose activities undermine state authority and creates disorder

Yields and qualities of pigeonpea varieties grown under smallholder farmers’ conditions in Eastern and Southern Africa

Pigeonpea is one of the few crops with a high potential for resource-poor farmers due to its complementary resource use when intercropped with maize. A three year comprehensive comparative study on the performance of six pigeonpea (Cajanus cajan) varieties on farmers’ fields in Eastern and Southern Africa where intercropping with maize is normal practice, was undertaken. The varieties were tested for accumulation of dry matter (DM), nitrogen (N) and phosphorus (P) in all above-ground organs for three years under farmers’ conditions. The study revealed that the latest introduced ICEAP 00040 outperformed all the other tested varieties (ICP 9145; ICEAP 00020, ICEAP 00053, ICEAP 00068, and a local variety called “Babati White”) under farmer-managed conditions. The harvest indices (HI), ranging from 0.08-0.15 on dry matter (DM) basis, were relatively low and unaffected (P>0.05) by the environmental variation. The N harvest index (NHI) was 0.28 and P harvest index (PHI) was 0.19. The better responses of ICEAP00040 to favourable conditions could however only be realised in a minority of cases as yields generally were low. These low yields are still a major challenge in African smallholder agriculture as pulses play an important role in soil fertility maintenance as well as in the household diets

Renewable energy, landfill gas and EfW: now, next and future

The United Kingdom (UK) has traditionally used landfill disposal as the predominant method of waste management. However, landfilling is unsustainable due to its harmful effects on the environment and public health. Under the Euorpean Union (EU) landfill directive, member nations are now required to divert biodegradable municipal waste (BMW) from landfills. The UK has also committed to the EU Renewable Energy Directive, which binds it to sourcing at least 15% of its energy mix from renewables by 2020. To meet these targets, the UK has to support alternative waste management options whilst achievingconsiderable deployment of renewables. This research considers the development of energy from waste (EfW) technologies and their potential contribution to UK's renewable energy targets. This study identifies the use of biomethane as road transport fuel and small-scale EfW deployment at community level as applications with huge potential benefits for the UK. These two options are easily Implemetable and could provide substantial savings in greenhouse gas (GHG) emissions. This study concludes that, depending on the pace of investment and availability of suitable feedstock, EfW technologies can contribute up to 50% of UK renewables target by 2020

Factors Influencing the Competitiveness of Cocoa Export of Ghana and Its Implication on Ghana’s Economy

This study examines the competitiveness of Ghana’s cocoa by assessing the performance and determinants of cocoa export from Ghana. Competitiveness of the country in cocoa exports was assessed using the Pearson's Correlation model and Linear Regression model using SPSS and STATA respectively. Time series data on Ghana’s cocoa production and exports and GDP from 2000 to 2014 were collected and analyzed using Pearson Correlation Statistics that were adopted in SPSS. The first analysis was to find the relationship between total cocoa products exports in US$(including raw cocoa beans and processed cocoa products) and factors such as the real world prices of cocoa (US$/MT) between 2000 and 2014, the gross domestic product of Ghana, the amount of Cocoa beans produced (in tonnes) and processed cocoa exports. The outcome of the analyses revealed that Ghana has comparative advantage in the exportation of cocoa, that Ghana is highly competitive in exports of cocoa beans, total cocoa products and processed cocoa exports. In spite of improvements observed in the country’s export performance over the past three decades, there is potential for further improvement, based on the SPSS and STATA results. The study proceeds to propose measures eminent in ensuring robust cocoa production and consequential exports. Keywords：Competitiveness, cocoa export, determinants, real world price, gross domestic price. DOI: 10.7176/JESD/10-6-05 Publication date:March 31st 201

Relationship between chlorophyll content in leaves of sorghum and pigeonpea determined by extraction method and by chlorophyll meter (SPAD-502)

The estimation of chlorophyll content in leaves by the chlorophyll meter (SPAD 502) is more convenient than by the extraction method for studies on photosynthesis or senescence where the total chlorophyll is estimated on the same leaf over time. This study was to test hypothesis that specific leaf weight (SLW) appears to be one of the factors determining SPAD index under different conditions. The influence of SLW on SPAD index and an improved simple method to determine chlorophyll content of sorghum and pigeonpea by a chlorophyll meter was studied. The results indicated that regression lines were significantly different between sorghum and pigeonpea, and at the vegetative and physiological maturity stages in each crop. Residuals of simple regressions calculated from all data of each crop were correlated with SLW. Multiple-regression with SPAD index as the dependent variable, and chlorophyll content and SLW as the independent variables gave the best estimation of chlorophyll content in leaves of sorghum and pigeonpea. These results suggest that SLW is an important factor affecting SPAD index and the influence of SLW on SPAD index can vary with crop species. Devices for estimating SLW could be incorporated into the chlorophyll meter to provide SPAD values adjusted for SLW. Further investigation is required on the influence of SLW on SPAD index for other crops

Tourism management synergies in Sub‐Saharan Africa

The purpose of this study is to assess how some governance dynamics, such as political stability and the rule of law, moderate the incidence of some macroeconomic factors (i.e., domestic investment and trade openness) on tourism development. The focus of this study is on 47 countries in sub‐Saharan Africa with data from 2002 to 2018, and the Generalized Method of Moments is employed as the empirical strategy. From the findings, synergy effects are apparent in the role of the rule of law in moderating domestic investment for tourism development in terms of tourism receipts. It follows that, for the sampled countries, promoting tourism development can be most effective if policies for enhancing domestic investment and promoting the rule of law are implemented simultaneously

The Impact of Socioeconomic Factors on Selected Practices by Small Livestock Producers in Alabama

Socioeconomic factors are generally believed to affect practices of small livestock producers. Yet, there has been limited research on the issue, especially in Alabama. This study, therefore, focused on the impact of socioeconomic factors on practices of small livestock producers in Alabama. Data were obtained from a convenience sample of 121 producers from South Central Alabama, and were analyzed using descriptive statistics and logit analysis. The socioeconomic factors reflected a higher proportion of part-time farmers; many more middle-aged persons, with at most a two-year/technical degree or some college education; and a higher proportion with $40,000 or less annual household income. A majority practiced rotational grazing; had parasite problems; used veterinary services; kept records, and nearly half conducted soil tests regularly. In addition, several socioeconomic factors had significant effects on selected practices; farming status had a significant effect on rotational grazing; education and income had significant effects on parasite problems; age had a significant effect on veterinary services; and race/ethnicity and education had significant effects on record keeping. The findings suggest that socioeconomic factors are important and must be considered in program implementation. Keywords: Socioeconomic Factors, Practices, Small Livestock Producer

A Loop Region in the N-Terminal Domain of Ebola Virus VP40 Is Important in Viral Assembly, Budding, and Egress

Ebola virus (EBOV) causes viral hemorrhagic fever in humans and can have clinical fatality rates of ~60%. The EBOV genome consists of negative sense RNA that encodes seven proteins including viral protein 40 (VP40). VP40 is the major Ebola virus matrix protein and regulates assembly and egress of infectious Ebola virus particles. It is well established that VP40 assembles on the inner leaflet of the plasma membrane of human cells to regulate viral budding where VP40 can produce virus like particles (VLPs) without other Ebola virus proteins present. The mechanistic details, however, of VP40 lipid-interactions and protein-protein interactions that are important for viral release remain to be elucidated. Here, we mutated a loop region in the N-terminal domain of VP40 (Lys127, Thr129, and Asn130) and find that mutations (K127A, T129A, and N130A) in this loop region reduce plasma membrane localization of VP40. Additionally, using total internal reflection fluorescence microscopy and number and brightness analysis we demonstrate these mutations greatly reduce VP40 oligomerization. Lastly, VLP assays demonstrate these mutations significantly reduce VLP release from cells. Taken together, these studies identify an important loop region in VP40 that may be essential to viral egress