Surveillance study of apparent life-threatening events (ALTE) in the Netherlands

SIDS and ALTE are different entities that somehow show some similarities. Both constitute heterogeneous conditions. The Netherlands is a low-incidence country for SIDS. To study whether the same would hold for ALTE, we studied the incidence, etiology, and current treatment of ALTE in The Netherlands. Using the Dutch Pediatric Surveillance Unit, pediatricians working in second- and third-level hospitals in the Netherlands were asked to report any case of ALTE presented in their hospital from January 2002 to January 2003. A questionnaire was subsequently sent to collect personal data, data on pregnancy and birth, condition preceding the incident, the incident itself, condition after the incident, investigations performed, monitoring or treatment initiated during admission, any diagnosis made at discharge, and treatment or parental support offered after discharge. A total of 115 cases of ALTE were reported, of which 110 questionnaires were filled in and returned (response rate 97%). Based on the national birth rate of 200,000, the incidence of ALTE amounted 0.58/1,000 live born infants. No deaths occurred. Clinical diagnoses could be assessed in 58.2%. Most frequent diagnoses were (percentages of the total of 110 cases) gastro-esophageal reflux and respiratory tract infection (37.3% and 8.2%, respectively); main symptoms were change of color and muscle tone, choking, and gagging. The differences in diagnoses are heterogeneous. In 34%, parents shook their infants, which is alarmingly high. Pre- and postmature infants were overrepresented in this survey (29.5% and 8.2%, respectively). Ten percent had recurrent ALTE. In total, 15.5% of the infants were discharged with a home monitor. In conclusion, ALTE has a low incidence in second- and third-level hospitals in the Netherlands. Parents should be systematically informed about the possible devastating effects of shaking an infant. Careful history taking and targeted additional investigations are of utmost importance

IFN-gamma Plays a Unique Role in Protection against Low Virulent Trypanosoma cruzi Strain

Background: T. cruzi strains have been divided into six discrete typing units (DTUs) according to their genetic background. These groups are designated T. cruzi I to VI. In this context, amastigotes from G strain (T. cruzi I) are highly infective in vitro and show no parasitemia in vivo. Here we aimed to understand why amastigotes from G strain are highly infective in vitro and do not contribute for a patent in vivo infection. Methodology/Principal Findings: Our in vitro studies demonstrated the first evidence that IFN-gamma would be associated to the low virulence of G strain in vivo. After intraperitoneal amastigotes inoculation in wild-type and knockout mice for TNF-alpha, Nod2, Myd88, iNOS, IL-12p40, IL-18, CD4, CD8 and IFN-gamma we found that the latter is crucial for controlling infection by G strain amastigotes. Conclusions/Significance: Our results showed that amastigotes from G strain are highly infective in vitro but did not contribute for a patent infection in vivo due to its susceptibility to IFN-gamma production by host immune cells. These data are useful to understand the mechanisms underlying the contrasting behavior of different T. cruzi groups for in vitro and in vivo infection.CAPES [3038.005295/2011-40]CAPESFAPEMIGFAPEMIG [APQ-00621-11]CNPqCNPqFAPESPFAPESP [10-50959-4

A Recombinant Protein Based on Trypanosoma cruzi P21 Enhances Phagocytosis

Background: P21 is a secreted protein expressed in all developmental stages of Trypanosoma cruzi. The aim of this study was to determine the effect of the recombinant protein based on P21 (P21-His(6)) on inflammatory macrophages during phagocytosis. Findings: Our results showed that P21-His(6) acts as a phagocytosis inducer by binding to CXCR4 chemokine receptor and activating actin polymerization in a way dependent on the PI3-kinase signaling pathway. Conclusions: Thus, our results shed light on the notion that native P21 is a component related to T. cruzi evasion from the immune response and that CXCR4 may be involved in phagocytosis. P21-His(6) represents an important experimental control tool to study phagocytosis signaling pathways of different intracellular parasites and particles.Fundacao de Amparo a Pesquisa do Estado de Minas Gerais [APQ-00621-11]Fundacao de Amparo a Pesquisa do Estado de Minas GeraisFundacao de Amparo a Pesquisa do Estado de Sao PauloFundacao de Amparo a Pesquisa do Estado de Sao PauloCoordenacao de Aperfeicoamento de Pessoal de Nivel Superior [23038005295/2011-40]Coordenacao de Aperfeicoamento de Pessoal de Nivel SuperiorConselho Nacional de Desenvolvimento Cientifico e TecnologicoConselho Nacional de Desenvolvimento Cientifico e Tecnologic

In iNOS and gp91 KO mice no trypomastigote progeny was detected.

<p>Wild type, iNOS (a, c) and gp91 KO (b, d) mice were given intraperitoneally 100 000 G strain amastigotes. Parasitemia values were monitored in mouse blood at 7, 12, 19 and 26 days post-inoculation ; survival was checked every day until 30 post-inoculation . (n = 5 mice per group).</p

Whatever gene (CD4, CD8, Nod2 and Myd88) deletion, trypomastigotes were never detected in mice bloodstream.

<p>Wild type and CD4 (a, e), CD8 (b, f) Nod2 (c, g) and Myd88 (d, h) knockout mice mice were given intraperitoneally 100,000 G strain amastigotes. Parasitemia values were monitored in mouse blood at 7, 12, 19 and 26 days post-inoculation; survival was checked every day until 30 post-inoculation . (n = 5 mice per group).</p

Monitoring presence of activated NK cells in bloodstream post amastigote intraperitoneal inoculation.

<p>Flow cytometry was performed with mononuclear cells prepared from mice left without any inoculation (a, b, c, d and e), and mice that were given intraperitoneal G strain amastiogotes at day-8 post-inoculation (a′, b′, c′, d′ and e′) at day-25 (b″, c″, d″ and e″). Note the higher percentage of activated NK cells at day-8 post-inoculation (p<0.01). Gates: L – lymphocytes and LGL – large granular lymphocytes (NK cells).</p

Subordination strategies in Tupian languages

Abstract: Assessing the internal coherence and constituency of language families often centers either around comparing certain form-meaning correspondences, or around identifying the presence or absence of linguistic features across the members of the family. The former approach is generally restricted to the lexicon. The latter approach focuses mostly on structural characteristics of language. In this paper we present an alternative approach to comparing grammatical systems between languages within a language family, which aims at bringing these two approaches and their results closer to each other. We look at subordination strategies in a sample of Tupian languages, taking constructions as the basic unit of comparison, treating them as form-meaning correspondences. The Tupian family offers an especially intriguing case for studying subordination strategies in the South American context, given its enormous geographical spread and the variety of contact situations involving its member languages. Major patterns of subordination strategies can be discerned across the family, e.g. strategies involving nominalization, verbal incorporation and other subtypes of verbal serialization, but there is also a great degree of variability between the different languages. By mapping the structural diversity onto the known genealogy and geographic distribution, we hope to shed more light on the history of the Tupian family and on the diffusability of subordination strategies

Inflamatory and IFN-γ treated naive macrophages impaired cell-cycling trypomastigotes differentiate from amastigotes.

<p>Amastigotes did not multiply in inflammatory peritoneal macrophages in an <i>ex-vivo</i> assay (<b>A</b>). Treatment with recombinant IFN-γ controlled in a dose dependent manner trypomastigotes release from bone marrow derived naive macrophages (<b>B</b>) (p<0.001).</p