The transcriptional repressor protein NsrR senses nitric oxide directly via a [2Fe-2S] cluster

The regulatory protein NsrR, a member of the Rrf2 family of transcription repressors, is specifically dedicated to sensing nitric oxide (NO) in a variety of pathogenic and non-pathogenic bacteria. It has been proposed that NO directly modulates NsrR activity by interacting with a predicted [Fe-S] cluster in the NsrR protein, but no experimental evidence has been published to support this hypothesis. Here we report the purification of NsrR from the obligate aerobe Streptomyces coelicolor. We demonstrate using UV-visible, near UV CD and EPR spectroscopy that the protein contains an NO-sensitive [2Fe-2S] cluster when purified from E. coli. Upon exposure of NsrR to NO, the cluster is nitrosylated, which results in the loss of DNA binding activity as detected by bandshift assays. Removal of the [2Fe-2S] cluster to generate apo-NsrR also resulted in loss of DNA binding activity. This is the first demonstration that NsrR contains an NO-sensitive [2Fe-2S] cluster that is required for DNA binding activity

The NIKA instrument: results and perspectives towards a permanent KID based camera for the Pico Veleta observatory

The New IRAM KIDs Array (NIKA) is a pathfinder instrument devoted to millimetric astronomy. In 2009 it was the first multiplexed KID camera on the sky; currently it is installed at the focal plane of the IRAM 30-meters telescope at Pico Veleta (Spain). We present preliminary data from the last observational run and the ongoing developments devoted to the next NIKA-2 kilopixels camera, to be commissioned in 2015. We also report on the latest laboratory measurements, and recent improvements in detector cosmetics and read-out electronics. Furthermore, we describe a new acquisition strategy allowing us to improve the photometric accuracy, and the related automatic tuning procedure.Comment: 24th International Symposium on Space Terahertz Technology, ISSTT 2013, April 8 to 10, 2013, Groningen, the Netherland

Complete Genome Sequence and Comparative Metabolic Profiling of the Prototypical Enteroaggregative Escherichia coli Strain 042

Background \ud Escherichia coli can experience a multifaceted life, in some cases acting as a commensal while in other cases causing intestinal and/or extraintestinal disease. Several studies suggest enteroaggregative E. coli are the predominant cause of E. coli-mediated diarrhea in the developed world and are second only to Campylobacter sp. as a cause of bacterial-mediated diarrhea. Furthermore, enteroaggregative E. coli are a predominant cause of persistent diarrhea in the developing world where infection has been associated with malnourishment and growth retardation. \ud \ud Methods \ud In this study we determined the complete genomic sequence of E. coli 042, the prototypical member of the enteroaggregative E. coli, which has been shown to cause disease in volunteer studies. We performed genomic and phylogenetic comparisons with other E. coli strains revealing previously uncharacterised virulence factors including a variety of secreted proteins and a capsular polysaccharide biosynthetic locus. In addition, by using Biolog™ Phenotype Microarrays we have provided a full metabolic profiling of E. coli 042 and the non-pathogenic lab strain E. coli K-12. We have highlighted the genetic basis for many of the metabolic differences between E. coli 042 and E. coli K-12. \ud \ud Conclusion \ud This study provides a genetic context for the vast amount of experimental and epidemiological data published thus far and provides a template for future diagnostic and intervention strategies

Latest NIKA results and the NIKA-2 project

NIKA (New IRAM KID Arrays) is a dual-band imaging instrument installed at the IRAM (Institut de RadioAstronomie Millimetrique) 30-meter telescope at Pico Veleta (Spain). Two distinct Kinetic Inductance Detectors (KID) focal planes allow the camera to simultaneously image a field-of-view of about 2 arc-min in the bands 125 to 175 GHz (150 GHz) and 200 to 280 GHz (240 GHz). The sensitivity and stability achieved during the last commissioning Run in June 2013 allows opening the instrument to general observers. We report here the latest results, in particular in terms of sensitivity, now comparable to the state-of-the-art Transition Edge Sensors (TES) bolometers, relative and absolute photometry. We describe briefly the next generation NIKA-2 instrument, selected by IRAM to occupy, from 2015, the continuum imager/polarimeter slot at the 30-m telescope.Comment: Proceedings of Low Temperature Detectors 15 (LTD-15), Pasadena, June 201

Detection of the tSZ effect with the NIKA camera

We present the first detection of the thermal Sunyaev-Zel'dovich (tSZ) effect from a cluster of galaxies performed with a KIDs (Kinetic Inductance Detectors) based instrument. The tSZ effect is a distortion of the black body CMB (Cosmic Microwave Background) spectrum produced by the inverse Compton interaction of CMB photons with the hot electrons of the ionized intra-cluster medium. The massive, intermediate redshift cluster RX J1347.5-1145 has been observed using NIKA (New IRAM KIDs arrays), a dual-band (140 and 240 GHz) mm-wave imaging camera, which exploits two arrays of hundreds of KIDs: the resonant frequencies of the superconducting resonators are shifted by mm-wave photons absorption. This tSZ cluster observation demonstrates the potential of the next generation NIKA2 instrument, being developed for the 30m telescope of IRAM, at Pico Veleta (Spain). NIKA2 will have 1000 detectors at 140GHz and 2x2000 detectors at 240GHz, providing in that band also a measurement of the linear polarization. NIKA2 will be commissioned in 2015.Comment: SF2A Proceedings 201

High resolution SZ observations at the IRAM 30-m telescope with NIKA

High resolution observations of the thermal Sunyaev-Zel'dovich (tSZ) effect are necessary to allow the use of clusters of galaxies as a probe for large scale structures at high redshifts. With its high resolution and dual-band capability at millimeter wavelengths, the NIKA camera can play a significant role in this context. NIKA is based on newly developed Kinetic Inductance Detectors (KIDs) and operates at the IRAM 30m telescope, Pico Veleta, Spain. In this paper, we give the status of the NIKA camera, focussing on the KID technology. We then present observations of three galaxy clusters: RX J1347.5-1145 as a demonstrator of the NIKA capabilities and the recent observations of CL J1226.9+3332 (z = 0.89) and MACS J0717.5+3745 (z = 0.55). We also discuss prospects for the final NIKA2 camera, which will have a 6.5 arcminute field of view with about 5000 detectors in two bands at 150 and 260 GHz

Transcriptomic analysis across nasal, temporal, and macular regions of human neural retina and RPE/choroid by RNA-Seq

AbstractProper spatial differentiation of retinal cell types is necessary for normal human vision. Many retinal diseases, such as Best disease and male germ cell associated kinase (MAK)-associated retinitis pigmentosa, preferentially affect distinct topographic regions of the retina. While much is known about the distribution of cell types in the retina, the distribution of molecular components across the posterior pole of the eye has not been well-studied. To investigate regional difference in molecular composition of ocular tissues, we assessed differential gene expression across the temporal, macular, and nasal retina and retinal pigment epithelium (RPE)/choroid of human eyes using RNA-Seq. RNA from temporal, macular, and nasal retina and RPE/choroid from four human donor eyes was extracted, poly-A selected, fragmented, and sequenced as 100 bp read pairs. Digital read files were mapped to the human genome and analyzed for differential expression using the Tuxedo software suite. Retina and RPE/choroid samples were clearly distinguishable at the transcriptome level. Numerous transcription factors were differentially expressed between regions of the retina and RPE/choroid. Photoreceptor-specific genes were enriched in the peripheral samples, while ganglion cell and amacrine cell genes were enriched in the macula. Within the RPE/choroid, RPE-specific genes were upregulated at the periphery while endothelium associated genes were upregulated in the macula. Consistent with previous studies, BEST1 expression was lower in macular than extramacular regions. The MAK gene was expressed at lower levels in macula than in extramacular regions, but did not exhibit a significant difference between nasal and temporal retina. The regional molecular distinction is greatest between macula and periphery and decreases between different peripheral regions within a tissue. Datasets such as these can be used to prioritize candidate genes for possible involvement in retinal diseases with regional phenotypes

The NIKA2 instrument, a dual-band kilopixel KID array for millimetric astronomy

NIKA2 (New IRAM KID Array 2) is a camera dedicated to millimeter wave astronomy based upon kilopixel arrays of Kinetic Inductance Detectors (KID). The pathfinder instrument, NIKA, has already shown state-of-the-art detector performance. NIKA2 builds upon this experience but goes one step further, increasing the total pixel count by a factor $\sim$10 while maintaining the same per pixel performance. For the next decade, this camera will be the resident photometric instrument of the Institut de Radio Astronomie Millimetrique (IRAM) 30m telescope in Sierra Nevada (Spain). In this paper we give an overview of the main components of NIKA2, and describe the achieved detector performance. The camera has been permanently installed at the IRAM 30m telescope in October 2015. It will be made accessible to the scientific community at the end of 2016, after a one-year commissioning period. When this happens, NIKA2 will become a fundamental tool for astronomers worldwide.Comment: Proceedings of the 16th Low Temperature Detectors workshop. To be published in the Journal of Low Temperature Physics. 8 pages, 4 figures, 1 tabl

NIKA 2: next-generation continuum/polarized camera at the IRAM 30 m telescope and its prototype

NIKA 2 (New Instrument of Kids Array) is a next generation continuum and polarized instrument successfully installed in October 2015 at the IRAM 30 m telescope on Pico-Veleta (Granada, Spain). NIKA 2 is a high resolution dual-band camera, operating with frequency multiplexed LEKIDs (Lumped Element Kinetic Inductance Detectors) cooled at 100 mK. Dual color images are obtained thanks to the simultaneous readout of a 1020 pixels array at 2 mm and 1140 x 2 pixels arrays at 1.15 mm with a final resolution of 18 and 12 arcsec respectively, and 6.5 arcmin of Field of View (FoV). The two arrays at 1.15 mm allow us to measure the linear polarization of the incoming light. This will place NIKA 2 as an instrument of choice to study the role of magnetic fields in the star formation process. The NIKA experiment, a prototype for NIKA 2 with a reduced number of detectors (about 400 LEKIDs) and FoV (1.8 arcmin), has been successfully operated at the IRAM 30 telescope in several open observational campaigns. The performance of the NIKA 2 polarization setup has been successfully validated with the NIKA prototype.Comment: 5 pages, 4 figures, proceeding for the conference: Extragalactic radio surveys 201

Non-parametric deprojection of NIKA SZ observations: Pressure distribution in the Planck-discovered cluster PSZ1 G045.85+57.71

The determination of the thermodynamic properties of clusters of galaxies at intermediate and high redshift can bring new insights into the formation of large-scale structures. It is essential for a robust calibration of the mass-observable scaling relations and their scatter, which are key ingredients for precise cosmology using cluster statistics. Here we illustrate an application of high resolution $(< 20$ arcsec) thermal Sunyaev-Zel'dovich (tSZ) observations by probing the intracluster medium (ICM) of the \planck-discovered galaxy cluster \psz\ at redshift $z = 0.61$, using tSZ data obtained with the NIKA camera, which is a dual-band (150 and 260~GHz) instrument operated at the IRAM 30-meter telescope. We deproject jointly NIKA and \planck\ data to extract the electronic pressure distribution from the cluster core ($R \sim 0.02\, R_{500}$) to its outskirts ($R \sim 3\, R_{500}$) non-parametrically for the first time at intermediate redshift. The constraints on the resulting pressure profile allow us to reduce the relative uncertainty on the integrated Compton parameter by a factor of two compared to the \planck\ value. Combining the tSZ data and the deprojected electronic density profile from \xmm\ allows us to undertake a hydrostatic mass analysis, for which we study the impact of a spherical model assumption on the total mass estimate. We also investigate the radial temperature and entropy distributions. These data indicate that \psz\ is a massive ($M_{500} \sim 5.5 \times 10^{14}$ M$_{\odot}$) cool-core cluster. This work is part of a pilot study aiming at optimizing the treatment of the NIKA2 tSZ large program dedicated to the follow-up of SZ-discovered clusters at intermediate and high redshifts. (abridged)Comment: 16 pages, 10 figure