Probing Dark Matter

Recent novel observations have probed the baryonic fraction of the galactic dark matter that has eluded astronomers for decades. Late in 1993, the MACHO and EROS collaborations announced in this journal the detection of transient and achromatic brightenings of a handful of stars in the Large Magellanic Cloud that are best interpreted as gravitational microlensing by low-mass foreground objects (MACHOS). This tantalized astronomers, for it implied that the population of cool, compact objects these lenses represent could be the elusive dark matter of our galactic halo. A year later in 1994, Sackett et al. reported the discovery of a red halo in the galaxy NGC 5907 that seems to follow the inferred radial distribution of its dark matter. This suggested that dwarf stars could constitute its missing component. Since NGC 5907 is similar to the Milky Way in type and radius, some surmised that the solution of the galactic dark matter problem was an abundance of ordinary low-mass stars. Now Bahcall et al., using the Wide-Field Camera of the recently repaired Hubble Space Telescope, have dashed this hope.Comment: 3 pages, Plain TeX, no figures, published as a News and Views in Nature 373, 191 (1995

Does shade improve light interception efficiency? A comparison among seedlings from shade-tolerant and -intolerant temperate deciduous tree species

• Here, we tested two hypotheses: shading increases light interception efficiency (LIE) of broadleaved tree seedlings, and shade-tolerant species exhibit larger LIEs than do shade-intolerant ones. The impact of seedling size was taken into account to detect potential size-independent effects on LIE. LIE was defined as the ratio of mean light intercepted by leaves to light intercepted by a horizontal surface of equal area. • Seedlings from five species differing in shade tolerance (Acer saccharum, Betula alleghaniensis, A. pseudoplatanus, B. pendula, Fagus sylvatica) were grown under neutral shading nets providing 36, 16 and 4% of external irradiance. Seedlings (1- and 2-year-old) were three-dimensionally digitized, allowing calculation of LIE. • Shading induced dramatic reduction in total leaf area, which was lowest in shade-tolerant species in all irradiance regimes. Irradiance reduced LIE through increasing leaf overlap with increasing leaf area. There was very little evidence of significant size-independent plasticity of LIE. • No relationship was found between the known shade tolerance of species and LIE at equivalent size and irradiance

Direct observation of molecular cooperativity near the glass transition

We describe direct observations of molecular cooperativity near the glass transition in poly-vinyl-acetate (PVAc), through nanometer-scale probing of dielectric fluctuations. Molecular clusters switched spontaneously between two to four distinct configurations, producing complex random-telegraph-signals (RTS). Analysis of the RTS and their power spectra shows that individual clusters exhibit both transient dynamical heterogeneity and non-exponential kinetics.Comment: 14 pages pdf, need Acrobat Reade

Light-like polygonal Wilson loops in 3d Chern-Simons and ABJM theory

We study light-like polygonal Wilson loops in three-dimensional Chern-Simons and ABJM theory to two-loop order. For both theories we demonstrate that the one-loop contribution to these correlators cancels. For pure Chern-Simons, we find that specific UV divergences arise from diagrams involving two cusps, implying the loss of finiteness and topological invariance at two-loop order. Studying those UV divergences we derive anomalous conformal Ward identities for n-cusped Wilson loops which restrict the finite part of the latter to conformally invariant functions. We also compute the four-cusp Wilson loop in ABJM theory to two-loop order and find that the result is remarkably similar to that of the corresponding Wilson loop in N=4 SYM. Finally, we speculate about the existence of a Wilson loop/scattering amplitude relation in ABJM theory.Comment: 37 pages, many figures; v2: references added, minor changes; v3: references added, sign error fixed and note adde

Transcriptional analysis of temporal gene expression in germinating Clostridium difficile 630 endospores.

Clostridium difficile is the leading cause of hospital acquired diarrhoea in industrialised countries. Under conditions that are not favourable for growth, the pathogen produces metabolically dormant endospores via asymmetric cell division. These are extremely resistant to both chemical and physical stress and provide the mechanism by which C. difficile can evade the potentially fatal consequences of exposure to heat, oxygen, alcohol, and certain disinfectants. Spores are the primary infective agent and must germinate to allow for vegetative cell growth and toxin production. While spore germination in Bacillus is well understood, little is known about C. difficile germination and outgrowth. Here we use genome-wide transcriptional analysis to elucidate the temporal gene expression patterns in C. difficile 630 endospore germination. We have optimized methods for large scale production and purification of spores. The germination characteristics of purified spores have been characterized and RNA extraction protocols have been optimized. Gene expression was highly dynamic during germination and outgrowth, and was found to involve a large number of genes. Using this genome-wide, microarray approach we have identified 511 genes that are significantly up- or down-regulated during C. difficile germination (p≤0.01). A number of functional groups of genes appeared to be co-regulated. These included transport, protein synthesis and secretion, motility and chemotaxis as well as cell wall biogenesis. These data give insight into how C. difficile re-establishes its metabolism, re-builds the basic structures of the vegetative cell and resumes growth

Fatal septicemia in a patient with cerebral lymphoma and an Amplatzer septal occluder: a case report

<p>Abstract</p> <p>Introduction</p> <p>The Amplatzer septal occluder is frequently used for percutaneous closure of an atrial septal defect. Complications include thrombosis and embolism, dislocation, cardiac perforation, and, rarely, infection. We report the case of a patient who had survived an occluder-related thromboembolism two years previously.</p> <p>Case presentation</p> <p>A 72-year-old Caucasian woman had received a septal occluder because of an atrial septal defect seven years ago. Two years ago, she underwent chemotherapy of a non-Hodgkin lymphoma, developed atrial fibrillation, and experienced a left-sided occluder thrombosis with stroke and peripheral embolism. Now, she presented with cerebral lymphoma, received glucocorticoids, and subsequently developed skin lesions. Swabs from the lesions and blood cultures were positive for methicillin-resistant <it>Staphylococcus aureus </it>and <it>Pseudomonas aeruginosa</it>. Endocarditis, however, was considered only two months later and echocardiography suggested aortic valve endocarditis. Despite antibiotic therapy, she died three days later because of septicemia, and no post-mortem investigation was carried out. It remains uncertain whether the septal occluder was endothelialized or infected and whether explantation might have changed the outcome.</p> <p>Conclusions</p> <p>If infections occur in patients with a septal occluder, endocarditis should be considered and echocardiography should be performed early. To prevent a fatal outcome, explantation of the septal occluder should be considered, especially in patients with problems that suggest delayed endothelialization. Post-mortem investigations, including bacteriologic studies, should be carried out in patients with a septal occluder in order to assess the focal and global long-term effects of these devices.</p

The Calcitonin and Glucocorticoids Combination: Mechanistic Insights into Their Class-Effect Synergy in Experimental Arthritis

PMCID: PMC3564948This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited