Active forms of tourism among lower and higher secondary students in Cracow

The purpose of this paper is to determine organizational forms (formal, non-formal) and the level of tourism activity among young people of secondary schools in Cracow depending on gender, the type of school and the education of the parents. The study included 1472 students of schools in Cracow. Physically active forms of tourism are undertaken mostly by girls – 63% rather than by boys – 52%. With increasing levels of education of the parents, the number of young people practicing active forms of tourism also increases. The undertaking of active forms of tourism varies, depending of the type of school attended. Young people in secondary (63,9%) and primary (65,7%) schools most often chose to go for tourism. In primary (50,1%) and secondary (39,4%) vocational schools they go least often. Active forms of tourism usually take the form of informal practice among friends and acquaintances (mostly hiking – 47%)

Next-generation sequencing of a combinatorial peptide phage library screened against ubiquitin identifies peptide aptamers that can inhibit the in vitro ubiquitin transfer cascade

Defining dynamic protein–protein interactions in the ubiquitin conjugation reaction is a challenging research area. Generating peptide aptamers that target components such as ubiquitin itself, E1, E2, or E3 could provide tools to dissect novel features of the enzymatic cascade. Next-generation deep sequencing platforms were used to identify peptide sequences isolated from phage-peptide libraries screened against Ubiquitin and its ortholog NEDD8. In over three rounds of selection under differing wash criteria, over 13,000 peptides were acquired targeting ubiquitin, while over 10,000 peptides were selected against NEDD8. The overlap in peptides against these two proteins was less than 5% suggesting a high degree in specificity of Ubiquitin or NEDD8 toward linear peptide motifs. Two of these ubiquitin-binding peptides were identified that inhibit both E3 ubiquitin ligases MDM2 and CHIP. NMR analysis highlighted distinct modes of binding of the two different peptide aptamers. These data highlight the utility of using next-generation sequencing of combinatorial phage-peptide libraries to isolate peptide aptamers toward a protein target that can be used as a chemical tool in a complex multi-enzyme reaction

Mitochondrial physiology

As the knowledge base and importance of mitochondrial physiology to evolution, health and disease expands, the necessity for harmonizing the terminology concerning mitochondrial respiratory states and rates has become increasingly apparent. The chemiosmotic theory establishes the mechanism of energy transformation and coupling in oxidative phosphorylation. The unifying concept of the protonmotive force provides the framework for developing a consistent theoretical foundation of mitochondrial physiology and bioenergetics. We follow the latest SI guidelines and those of the International Union of Pure and Applied Chemistry (IUPAC) on terminology in physical chemistry, extended by considerations of open systems and thermodynamics of irreversible processes. The concept-driven constructive terminology incorporates the meaning of each quantity and aligns concepts and symbols with the nomenclature of classical bioenergetics. We endeavour to provide a balanced view of mitochondrial respiratory control and a critical discussion on reporting data of mitochondrial respiration in terms of metabolic flows and fluxes. Uniform standards for evaluation of respiratory states and rates will ultimately contribute to reproducibility between laboratories and thus support the development of data repositories of mitochondrial respiratory function in species, tissues, and cells. Clarity of concept and consistency of nomenclature facilitate effective transdisciplinary communication, education, and ultimately further discovery

Mitochondrial physiology

As the knowledge base and importance of mitochondrial physiology to evolution, health and disease expands, the necessity for harmonizing the terminology concerning mitochondrial respiratory states and rates has become increasingly apparent. The chemiosmotic theory establishes the mechanism of energy transformation and coupling in oxidative phosphorylation. The unifying concept of the protonmotive force provides the framework for developing a consistent theoretical foundation of mitochondrial physiology and bioenergetics. We follow the latest SI guidelines and those of the International Union of Pure and Applied Chemistry (IUPAC) on terminology in physical chemistry, extended by considerations of open systems and thermodynamics of irreversible processes. The concept-driven constructive terminology incorporates the meaning of each quantity and aligns concepts and symbols with the nomenclature of classical bioenergetics. We endeavour to provide a balanced view of mitochondrial respiratory control and a critical discussion on reporting data of mitochondrial respiration in terms of metabolic flows and fluxes. Uniform standards for evaluation of respiratory states and rates will ultimately contribute to reproducibility between laboratories and thus support the development of data repositories of mitochondrial respiratory function in species, tissues, and cells. Clarity of concept and consistency of nomenclature facilitate effective transdisciplinary communication, education, and ultimately further discovery

Active forms of tourism among lower and higher secondary students in Cracow

Tyt. z nagłówka.Bibliogr. s. 20.The purpose of this paper is to determine organizational forms (formal, non-formal) and the level of tourism activity among young people of secondary schools in Cracow depending on gender, the type of school and the education of the parents. The study included 1472 students of schools in Cracow. Physically active forms of tourism are undertaken mostly by girls - 63% rather than by boys - 52%. With increasing levels of education of the parents, the number of young people practicing active forms of tourism also increases. The undertaking of active forms of tourism varies, depending of the type of school attended. Young people in secondary (63,9%) and primary (65,7%) schools most often chose to go for tourism. In primary (50,1%) and secondary (39,4%) vocational schools they go least often. Active forms of tourism usually take the form of informal practice among friends and acquaintances (mostly hiking - 47%).Dostępny również w formie drukowanej.KEYWORDS: active forms of tourism, young people

Assessment of somatic and motor development level of 10-12 year old children

SUMMARY Introduction. Therefore, the main aim of this dissertation is an assessment of the somatic and motor development of ten to twelve-year-old children from Polish families who live in London and a comparison of the results with the analogical measurements children live in Poland. Material and methods. The material of the research are the results of the measurements of 113 children of Polish origin who live in London and 151 children who live in Poland. The research was performed in the autumn of 2012. Chosen somatic features (body height, body mass, lean body mass – LBM, the percentage of fat in the body) and the level of motor skills (measured by the use of chosen tests of the European Physical Fitness Test – EUROFIT: running speed, abdomen muscles force, shoulders force, static force, spine flexibility) were analyzed. Results. There are significant differences between the body composition of children from Polish families who live in London and of those who live in Poland. Eleven and twelve-year-old children from London are characterized by a significantly higher percentage of fat in the total body mass than the children who live in Poland. Although children from London do not have a lower level of body height and mass than the children from Poland, they are characterized by a significantly lower level of motor skills, which was visible especially in case of speed, power endurance, explosive power and flexibility.   STRESZCZENIE Wstęp. Za główny cel pracy przyjęto próbę oceny poziomu rozwoju somatycznego i motorycznego 10–12-letnich dzieci z rodzin polskich mieszkających Londynie oraz porównanie wyników badań z analogicznymi pomiarami dzieci mieszkających w Polsce. Materiał i metody badań. Materiał opracowania stanowią wyniki pomiarów 113 dzieci pochodzenia polskiego mieszkających w Londynie oraz 151 dzieci mieszkających w Polsce. Badania przeprowadzono jesienią 2012 roku. Analizie poddano poziom wybranych cech somatycznych (wysokość ciała, masę ciała, masę ciała szczupłego – LBM, procentową zawartość tłuszczu w organizmie) oraz poziom sprawności motorycznej mierzonej przy zastosowaniu wybranych prób Europejskiego Testu Sprawności Fizycznej – EUROFIT (szybkość biegowa, siła mięśni brzucha, siła ramion, siła statyczna, gibkość kręgosłupa). Wnioski. Wystąpiły wyraźne różnice w zakresie składu ciała pomiędzy dziećmi z rodzin polskich mieszkających w Wielkiej Brytanii a badanymi w kraju. U dziewcząt i chłopców w wieku 11 i 12 lat z Londynu znacząco większy niż u rówieśników z polskiej grupy porównawczej był udział masy tłuszczu w ogólnej masie ciała. Dzieci mieszkające w Londynie, choć nie ustępowały poziomem wysokości i masy ciała rówieśnikom z Polski to charakteryzowały się znacznie niższym poziomem sprawności motorycznej, co szczególnie widoczne było w zakresie szybkości, wytrzymałości siłowej, a także siły eksplozywnej i gibkości

WARTOŚCI KULTURY FIZYCZNEJ W GRUPIE SPORTOWCÓW UPRAWIAJĄCYCH SPORTY WALKI

Podczas badania zastosowano między innymi skalę wartości schelerowskich, koncentrując się jedynie na analizie wartości kultury fizycznej. Oszacowano również podstawowe statystyki rozkładu danych oraz siłę zależności korelacyjnych między wartościami. Wyniki potwierdzają rangę edukacji oraz dotychczasowych doświadczeń, w tym doświadczeń sportowych dorosłych respondentów, we wzmacnianiu ich pozytywnego nastawienia wobec wartości kultury fizycznej