A comparison of younger and older couples coping with breast cancer : examining the relationship among mutuality, dyadic coping, and quality of life of patients and partners.

Background Despite the extensive literature available about the psychosocial adaptation to breast cancer, the experience of younger women and their partners has been mostly neglected by the research. While younger couples have been identified at higher risk because of less collaborative behaviors, higher vulnerability to distress, and poorer quality of life, in most contributions couples are treated as a homogeneous group. This study was then designed to examine the differential impact of the illness on younger dyads, by comparing them to a group of older couples. A significant gap in the literature about dyadic coping was also addressed, which is the understanding of how dyadic coping originates and it is associated with higher quality of life. Methods Participants have been recruited among newly diagnosed women with early-stage breast cancer, for a total of 86 couples. Patients below the age of 45 and their partners were considered younger couples and compared to the remaining 51 older couples. The Actor Partner Interdependence Model (APIM) was used to examine actor and partner effects of mutuality on dyadic coping. A simple mediation model tested whether dyadic coping mediates the relationship between mutuality and quality of life. Summary of Findings Younger women reported significantly worse quality of life, with worse functioning in physical, social and emotional domains than older patients. Similarly, younger partners experienced higher illness intrusiveness, worse emotional well-being, and maladaptive dyadic coping. Younger couples’ positive and negative coping styles were the result of both actor and partner effects of mutuality, indicating higher interdependence in their relationship. Finally, negative dyadic coping mediated the relationship between mutuality and quality of life for younger patients and older partners. Conclusions Younger couples’ adaptation to breast cancer is significantly compromised not only because of the more negative impact of the illness on the individual’s well-being, but also as a result of higher reciprocal influence of mutuality in predicting both adaptive and maladaptive dyadic coping behaviors. Future studies should continue to examine the developmental trajectory of dyadic coping over the life-span and psychosocial interventions should be developed to promote positive adaptation to cancer in a time of higher vulnerability

Topological Properties of Co-Occurrence Networks in Published Gene Expression Signatures

Meta-analysis of high-throughput gene expression data is often used for the interpretation of proprietary gene expression data sets. We have recently shown that co-occurrence patterns of gene expression in published cancer-related gene expression signatures are reminiscent of several cancer signaling pathways. Indeed, significant co-occurrence of up to ten genes in published gene expression signatures can be exploited to build a co-occurrence network from the sets of co-occurring genes (“co-occurrence modules”). Such co-occurrence network is represented by an undirected graph, where single genes are assigned to vertices and edges indicate that two genes are significantly co-occurring. Thus, graph-cut methods can be used to identify groups of highly interconnected vertices (“network communities”) that correspond to sets of genes that are significantly co-regulated in human cancer. Here, we investigate the topological properties of co-occurrence networks derived from published gene expression signatures and show that co-occurrence networks are characterized by scale-free topology and hierarchical modularity. Furthermore, we report that genes with a “promiscuous” or a “faithful” co-occurrence pattern can be distinguished. This behavior is reminiscent of date and party hubs that have been identified in protein-protein interaction networks

NUMERICAL EVALUATION OF THE TEMPORAL VARIATION OF VIBRATIONS INDUCED BY UNDERGROUND TRAINS

The change in the dynamic response of shallow soils as caught by two geophysical test campaigns is exploited to numerically predict the variation in the ground borne vibrations induced by the passage of underground trains. Multiple causes may lead to a variation in the perception of vibrations over time: from an increase in the train load to the roughness of the railway track, from the increase of the train speed to the modification of the dynamic response of the surrounding soil. In the present study, special attention is devoted to the effect of the hydrological regime on the latter. Two scenarios were calibrated on the results of repeated geodynamic tests on the same site in Milano but at two different times. The two investigations revealed differences in the dynamic response of above-ground soils which can be related to different saturation profiles. The passage of a typical convoy is simulated in the time domain. Differences between the two scenarios are highlighted together with the role played by the static axle load of the train. Furthermore, comparisons with recorded accelerations are provided to validate the model

A numerical assessment of variable saturation of the upper layers on the ground borne vibrations from underground trains: A case history

Ground borne vibrations generated by the passage of underground trains may change over time due to objective causes, such as increasing weight and speed of trains or ageing of the infrastructure components, as well as a variation in the dynamic response of the soil surrounding the tunnel. Among the possible causes of changes in the soil dynamic response, its hydrologic state has been seldom investigated. In this contribution, the role played by the conditions of the soil above the water table is addressed, starting from a case history in the city of Milano. Two-dimensional plane strain numerical models have been developed for the infrastructure. The models were calibrated on the results of two geophysical investigations performed at the same site in the city centre, but at two different times, which allowed distinguishing different dynamic responses. The system was excited by a synthetic load time history, matching a reference dynamic load spectrum included in Italian recommendations. Limitations of using this input on a 2D plane strain model were assessed by comparing the computed vibrations with experimental acceleration records collected on the tunnel. The results of the two numerical models are compared with those of a simulation performed assuming fully dry conditions above the water table. Overall, the set of analyses shows that even small changes in the dynamic response of the soil, interpretated as a consequence of variable saturation, may result in a change of a few decibels in the acceleration levels. Much larger accelerations are predicted on average with the simpler dry model, clearly showing the advantages of a more accurate modelling strategy

Spatio-Temporal Changes of Extracellular Matrix (ECM) Stiffness in the Development of the Leech Hirudo verbana

The invertebrate leech Hirudo verbana represents a powerful experimental animal model for improving the knowledge about the functional interaction between the extracellular matrix (ECM) and cells within the tissue microenvironment (TME), and the key role played by ECM stiffness during development and growth. Indeed, the medicinal leech is characterized by a simple anatomical organization reproducing many aspects of the basic biological processes of vertebrates and in which a rapid spatiotemporal development is well established and easily assessed. Our results show that ECM structural organization, as well as the amount of fibrillar and non-fibrillar collagen are deeply different from hatching leeches to adult ones. In addition, the changes in ECM remodelling occurring during the different leech developmental stages, leads to a gradient of stiffness regulating both the path of migratory cells and their fates. The ability of cells to perceive and respond to changes in ECM composition and mechanics strictly depend on nuclear or cytoplasmic expression of Yes-Associated Protein 1 (YAP1), a key mediator converting mechanical signals into transcriptional outputs, expression, and activation

Dyadic Coping Across the Lifespan: A Comparison Between Younger and Middle-Aged Couples With Breast Cancer

The association between dyadic coping and adjustment to cancer has been well-established. However, a significant gap in the literature is the understanding of how the life stage of couples may influence their dyadic coping and the accompanying quality of life. Although younger couples have been identified at higher risk for poor coping because of less collaborative behaviors and higher vulnerability to stress, only a limited number of studies have addressed younger women's coping with breast cancer in the context of close relationships. The present study addressed the differential impact of the illness on the quality of life and dyadic coping behaviors of younger and middle-aged dyads and the influence of relational mutuality on couples' coping in the two groups. A sample of 86 couples participated in a cross-sectional study; 35 younger couples were compared to 51 middle-aged dyads. Patients and partners completed measures of quality of life, dyadic coping, and mutuality. Independent-samples t-tests were used to examine differences in the two groups, while the Actor-Partner Interdependence Model (APIM) identified actor and partner effects of relational mutuality on dyadic coping. Younger women and their partners reported statistically significant worse quality of life and dyadic coping scores than the middle-age group. For younger couples, positive and negative coping styles were the result of both actor and partner effects of mutuality. The study highlighted the more negative impact of breast cancer on the quality of life of younger patients and partners. It also revealed a stronger influence of each partner's relational mutuality compared to the middle-age group in predicting both adaptive and maladaptive coping behavior. Future studies should continue to examine the developmental trajectory of dyadic coping across the lifespan in order to develop psychosocial interventions to promote younger dyads' coping efforts

RNASET2 as a tumor antagonizing gene in a melanoma cancer model

The RNASET2 gene, mapped in 6q27, was previously found to exert control of tumorigenesis in an ovarian cancer system. We present here results indicating a similar control in a melanoma cancer model. Thus, this gene is most likely involved in a common general pathway of tumorigenesis. Moreover, its antitumorigenic activity is manifested in vivo but not in vitro, suggesting that this gene belongs to the growing category of tumor antagonizing/malignancy suppressor genes. A possible role of RNASET2 in the activation of a senescence program, whose responsible locus was mapped in the same chromosomal 6q27 region, seems to be inconsistent with our data

Production of laccase by Botrytis cinerea and fermentation studies with strain F226

After induction, seven strains ofBotrytis cinerea released into the culture broth considerable amounts of laccase in a brief production time. The set-up of a suitable production process was studied with a selected strain in a 10-L fermenter. The optimum fermentation conditions were a 3% inoculum with a high degree of sporulation, a simple medium containing 20 g L−1 of glucose and 2 g L−1 of yeast extract at pH 3.5, 2 g L−1 gallic acid as inducer, added after 2 days of growth, an agitation speed of 300 rpm, an aeration rate of 1.2 vvm and a temperature of 24°C. By optimizing the culture conditions, the enzyme activity reached 28 U ml−1 in 5 days with a specific activity of 560 U mg−1 protein. The best procedure to obtain a suitable crude enzyme preparation was concentration of the supernatant medium to 10% of the initial volume by ultrafiltration, followed by a fractional precipitation with ethanol. The optimum pH and temperature for laccase activity were 5.5 and 40°C, respectively, with syringaldazine as the substrate