Uncertainty Quantification for Maxwell's Eigenproblem based on Isogeometric Analysis and Mode Tracking

The electromagnetic field distribution as well as the resonating frequency of various modes in superconducting cavities used in particle accelerators for example are sensitive to small geometry deformations. The occurring variations are motivated by measurements of an available set of resonators from which we propose to extract a small number of relevant and independent deformations by using a truncated Karhunen-Lo\`eve expansion. The random deformations are used in an expressive uncertainty quantification workflow to determine the sensitivity of the eigenmodes. For the propagation of uncertainty, a stochastic collocation method based on sparse grids is employed. It requires the repeated solution of Maxwell's eigenvalue problem at predefined collocation points, i.e., for cavities with perturbed geometry. The main contribution of the paper is ensuring the consistency of the solution, i.e., matching the eigenpairs, among the various eigenvalue problems at the stochastic collocation points. To this end, a classical eigenvalue tracking technique is proposed that is based on homotopies between collocation points and a Newton-based eigenvalue solver. The approach can be efficiently parallelized while tracking the eigenpairs. In this paper, we propose the application of isogeometric analysis since it allows for the exact description of the geometrical domains with respect to common computer-aided design kernels, for a straightforward and convenient way of handling geometrical variations and smooth solutions

The SPO1-related bacteriophages

A large and diverse group of bacteriophages has been termed ‘SPO1-like viruses'. To date, molecular data and genome sequences are available for Bacillus phage SPO1 and eight related phages infecting members of other bacterial genera. Many additional bacteriophages have been described as SPO1-related, but very few data are available for most of them. We present an overview of putative ‘SPO1-like viruses' and shall discuss the available data in view of the recently proposed expansion of this group of bacteriophages to the tentative subfamily Spounavirinae. Characteristics of SPO1-related phages include (a) the host organisms are Firmicutes; (b) members are strictly virulent myoviruses; (c) all phages feature common morphological properties; (d) the phage genome consists of a terminally redundant, non-permuted dsDNA molecule of 127-157kb in size; and (e) phages share considerable amino acid homology. The number of phages isolated consistent with these parameters is large, suggesting a ubiquitous nature of this group of viruse

Metallorganische Lewis-Säuren. Metallkomplexe mit schwach koordinierten Liganden

Die Tetrafluoroborato- und Hexafluoroantimonato-Komplexe (π-C5H5)(CO)3MoX (X = FBF3, FSbF5) setzen sich mit (π-C5H5)(CO)3MoCH3, (π-C5H5)(CO)2FeCH3 oder (π-C5H5)(CO)2FeCOCH3 und cis-, trans-Ph3P(CO)4MnCOCH3 zu [(π-C5H5)(CO)2Mo(μ2-η2-COCH3)Mo(CO)2(π-C5H5)]+ BF4− (I), [(π-C5H5)(CO)2Fe - C(CH3)O-Mo(CO)3(π-C5H5)]+ SbF6− (II) und [(Ph3P)(CO)4Mn-C(CH3)O-Mo(CO)3(π-C5H5)]+ BF4− (III) um

Characterization of a CdZnTe detector for a low-power CubeSat application

We report spectral and imaging performance of a pixelated CdZnTe detector custom designed for the MeVCube project: a small Compton telescope on a CubeSat platform. MeVCube is expected to cover the energy range between 200 keV and 4 MeV, with a sensitivity comparable to the one of the last generation of larger satellites. In order to achieve this goal, an energy resolution of few percent in full width at half maximum (FWHM) and a 3-D spatial resolution of few millimeters for the individual detectors are needed. The severe power constraints present in small satellites require very low power read-out electronics for the detector. Our read-out is based on the VATA450.3 ASIC developed by Ideas, with a power consumption of only 0.25 mW/channel, which exhibits good performance in terms of dynamic range, noise and linearity. A 2.0 cm× 2.0 cm× 1.5 cm CdZnTe detector, with a custom 8 × 8 pixel anode structure read-out by a VATA450.3 ASIC, has been tested. A preliminary read-out system for the cathode, based on a discrete Amptek A250F charge sensitive pre-amplifier and a DRS4 ASIC, has been implemented. An energy resolution around 3% FWHM has been measured at a gamma energy of 662 keV; at 200 keV the average energy resolution is 6.5%, decreasing to ≲ 2% at energies above 1 MeV. A 3-D spatial resolution of ≈ 2 mm is achieved in each dimension.Peer Reviewe

Characterization of a CdZnTe detector for a low-power CubeSat application

We report spectral and imaging performance of a pixelated CdZnTe detector custom designed for the \emph{MeVCube} project: a small Compton telescope on a CubeSat platform. \emph{MeVCube} is expected to cover the energy range between $200\;\mathrm{keV}$ and $4\;\mathrm{MeV}$, with performance comparable to the last generation of larger satellites. In order to achieve this goal, an energy resolution of few percent in full width at half maximum (FWHM) and a $3$-D spatial resolution of few millimeters for the individual detectors are needed. The severe power constraints present in small satellites require very low power read-out electronics for the detector. Our read-out is based on the VATA450.3 ASIC developed by \emph{Ideas}, with a power consumption of only $0.25\;\mathrm{mW/channel}$, which exhibits good performance in terms of dynamic range, noise and linearity. A $2.0\;\mathrm{cm} \times 2.0\;\mathrm{cm} \times 1.5\;\mathrm{cm}$ CdZnTe detector, with a custom $8 \times 8$ pixel anode structure read-out by a VATA450.3 ASIC, has been tested. A preliminary read-out system for the cathode, based on a discrete \emph{Amptek} A250F charge sensitive pre-amplifier and a DRS4 ASIC, has been implemented. An energy resolution around $3\%$ FWHM has been measured at a gamma energy of $662\;\mathrm{keV}$; at $200\;\mathrm{keV}$ the average energy resolution is $6.5\%$, decreasing to $\lesssim 2\%$ at energies above $1\;\mathrm{MeV}$. A $3$-D spatial resolution of $\approx 2\,\mathrm{mm}$ is achieved

A new and reliable culture system for superficial low-grade urothelial carcinoma of the bladder

Several bladder cancer culture systems have been developed in recent years. However, reports about successful primary cultures of superficial urothelial carcinomas (UC) are sparse. Based on the specific growth requirements of UC described previously, we developed a new and reliable culture system for superficial low-grade UC. Between November 2002 and April 2006, 64 primary cultures of bladder cancer specimens were performed. After incubating the specimens overnight in 0.1% ethylenediaminetetraacetic acid solution, tumour cells could easily be separated from the submucosal tissue. Subsequently, cells were seeded in a low-calcium culture medium supplemented with 1% serum, growth factors, non-essential amino acids and glycine. The malignant origin of the cultured cells was demonstrated by spectral karyotyping. Overall culture success rate leading to a homogenous tumour cell population without fibroblast contamination was 63%. Culture success could be remarkably enhanced by the addition of glycine to the culture medium. Interestingly, 86.4% of pTa tumours were cultured successfully compared to only 50% of the pT1 and 38% of advanced stage tumours, respectively. G1 and G2 tumours grew significantly better than G3 tumours (86, 73 and 41%, respectively). Up to three passages of low-grade UC primary cultures were possible. We describe a new and reliable culture system, which is highly successful for primary culture and passage of low-grade UC of the bladder. Therefore, this culture system can widely be used for functional experiments on early stage bladder cance

Comparative analysis of 37 Acinetobacter bacteriophages

© 2017 by the authors. Licensee MDPI, Basel, Switzerland. Members of the genus Acinetobacter are ubiquitous in the environment and the multipledrug resistant species A. baumannii is of significant clinical concern. This clinical relevance is currently driving research on bacterial viruses infecting A. baumannii, in an effort to implement phage therapy and phage-derived antimicrobials. Initially, a total of 42 Acinetobacter phage genome sequences were available in the international nucleotide sequence databases, corresponding to a total of 2.87 Mbp of sequence information and representing all three families of the order Caudovirales and a single member of the Leviviridae. A comparative bioinformatics analysis of 37 Acinetobacter phages revealed that they form six discrete clusters and two singletons based on genomic organisation and nucleotide sequence identity. The assignment of these phages to clusters was further supported by proteomic relationships established using OrthoMCL. The 4067 proteins encoded by the 37 phage genomes formed 737 groups and 974 orphans. Notably, over half of the proteins encoded by the Acinetobacter phages are of unknown function. The comparative analysis and clustering presented enables an updated taxonomic framing of these clades

The genome and proteome of a virulent Escherichia coli O157:H7 bacteriophage closely resembling Salmonella phage Felix O1

Based upon whole genome and proteome analysis, Escherichia coli O157:H7-specific bacteriophage (phage) wV8 belongs to the new myoviral genus, "the Felix O1-like viruses" along with Salmonella phage Felix O1 and Erwinia amylovora phage φEa21-4. The genome characteristics of phage wV8 (size 88.49 kb, mol%G+C 38.9, 138 ORFs, 23 tRNAs) are very similar to those of phage Felix O1 (86.16 kb, 39.0 mol%G+C, 131 ORFs and 22 tRNAs) and, indeed most of the proteins have their closest homologs within Felix O1. Approximately one-half of the Escherichia coli O157:H7 mutants resistant to phage wV8 still serotype as O157:H7 indicating that this phage may recognize, like coliphage T4, two different surface receptors: lipopolysaccharide and, perhaps, an outer membrane protein