42 research outputs found

    Measuring \u3b2-galactosidase activity at pH 6 with a differential pH sensor

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    The \u3b2-Galactosidase activity at pH 6 is used as a cellular marker to identify senescent cell cultures. The classic method to identify this enzymatic activity is using cytochemical staining with X-Gal after 16 hrs. In this work, a differential pH sensor was used to measure \u3b2- Galactosidase activity at pH 6. The measurement is easy and only takes 3 min

    Measuring β-galactosidase activity at pH 6 with a differential pH sensor

    No full text
    The β-Galactosidase activity at pH 6 is used as a cellular marker to identify senescent cell cultures. The classic method to identify this enzymatic activity is using cytochemical staining with X-Gal after 16 hrs. In this work, a differential pH sensor was used to measure β- Galactosidase activity at pH 6. The measurement is easy and only takes 3 min
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