Synthesis, characterization, E/Z-isomerization, DFT, optical and 1BNA docking of new Schiff base derived from naphthalene-2-sulfonohydrazide

The novel N'-((1H-indol-2-yl)methylene)naphthalene-2-sulfono-hydrazide Schiff base (S.B.) ligand was produced via condensation of 1H-indole-2-carbaldehyde with naphthalene-2-sulfonylhydrazide in a high yield. S.B. was then examined using several physicochemical techniques, and the steric structure was consequently confirmed. The highest B3LYB/DFT level theory was used to optimize the 3D E/Z-isomers structure. The stereoisomers computation demonstrated slightly stable E isomers outperformed by Z-one. The ligand displayed decent photosynthetic mid-bandgap semiconductors optical activate range material, and both the E and Z isomers bound to 1BNA DNA recorded many interactions

Sulfur Nanoparticle as an Effective HEK-293 Anticancer Agent

The preparation of sulfur nanoparticles (S-NPs) by a fast precipitation low-temperature approach using hydrochloric acid, sodium thiosulfate, and tetrabutylammonium bromide (TOAB) as a surfactant and stabilized has been reported in this work. The atomic content and purity of the S-NPs were supported by Energy Dispersive X-ray (EDX), and scanning electron microscopy (SEM) imaging supported the morphology and demonstrated nanoparticle aggregation. The progress of S-NPs preparation was monitored via UV-vis and optical activities behavior. The structure and the size of S-NPs were examined via powder X-Ray diffraction (PXRD) analysis. Transmission electron microscope (TEM) conforms the prepared S-NPs are in homogenous nano-sized with a value of 7-10 nm. The thermal stability of the desired S-NPs matrix was also determined by TG/DTG measurements. S-NPs' in vitro cytotoxic activities were evaluated against the HL-60 acute myeloid leukemia cell line, the HEK-293 kidney carcinoma cell line, and the HT-29 colon cancer cell line. The cytotoxicity of HEK-293 cell lines treated with S-NPs was higher than that of the other cell lines, according to the MTT assay

Synthesis, optimization, DFT/TD-DFT and COX/LOX docking of new Schiff base N'-((9-ethyl-9H-carbazol-1-yl)methylene)naphthalene-2-sulfonohydrazide

Condensing naphthalene-2-sulfonylhydrazide with 9-ethyl-9H-carbazole-1-carbaldehyde enabled to prepare the N'-((9-ethyl-9H-carbazol-1-yl)methylene)naphthalene-2-sulfonohydrazide as new Schiff base (S.B.)  ligand with a high yield. A wide range of physicochemical measurements, such as CHN-elemental analysis, UV-Vis., NMR, FT-IR, and mass spectrometry (ESI-MS) were used to extensively investigate the structural properties of the prepared S.B. ligand. Moreover, the highest level of B3LYB/DFT also served to optimize the 3D structure of the S.B, the experimental optical UV-Vis absorption measurements was compared to the theoretical DFT/TD-DFT study under identical conditions using the same solvent. Furthermore, the anti-inflammatory possible future capability was evaluated via the LOX/COX molecular docking against the desired S.B. ligand

Antibacterial synergy of Tritirachium oryzae-produced silver nanoparticles with different antibiotics and essential oils derived from Cupressus sempervirens and Asteriscus graveolens (Forssk)

Purpose: To carry out eco-friendly biosynthesis of fungi-derived silver nanoparticles (AgNPs) and investigate their antibacterial synergies with essential oils (EOs) of Asteriscus graveolens (Forssk.) Less. and Cupressus sempervirens. Methods: Biosynthesis of AgNPs was carried out using a cell-free filtrate of Tritirachium oryzae. The biosynthesized AgNPs characteristics were assessed using different methods, including ultravioletvisible spectrophotometry (UV), Fourier-transform infrared spectroscopy (FTIR), scanning electron microscopy (SEM) with energy-dispersive x-ray spectroscopy (EDS) and transmission electron microscopy (TEM). Results: Obvious synergistic effects were observed between AgNPs and chloramphenicol, vancomycin, nitrofurantoin or tetracycline with Pseudomonas aeruginosa, through increases in fold area of inhibition (IFAs) within the range of 2.4 to 9.0. Synergistic interactions were also seen between AgNPs and the antibiotics used, depending on the strain. Increase in IFA ranged from 1- to 3-fold for S. aureus, E. coli and P. aeruginosa. Similarly, combinations of AgNPs, EO of A. graveolens and cefotaxime, nitrofurantoin or amoxicillin against P. aeruginosa led to 10-, 3- and 10-fold synergy, respectively. In contrast, the use of AgNPs and trimethoprim, tetracycline or amoxicillin against E. coli led to 1 to 6-fold synergy. The best synergistic capacity resulted from AgNPs and the EO of C. sempervirens and trimethoprim against S. epidermidis, which yielded 29-fold increase in IFA. The use of combination of AgNPs and vancomycin against P. aeruginosa led to 16.4-fold enhancement of IFA. Conclusion: The findings can potentially lead to the development of a new perception of antibacterial agents (innovative medications) involving the incorporation of nanoparticles (NPs) or new materials that potentially synergize with antibiotics, NPs and the EOs of different plants

Impact of opioid-free analgesia on pain severity and patient satisfaction after discharge from surgery: multispecialty, prospective cohort study in 25 countries

Background: Balancing opioid stewardship and the need for adequate analgesia following discharge after surgery is challenging. This study aimed to compare the outcomes for patients discharged with opioid versus opioid-free analgesia after common surgical procedures.Methods: This international, multicentre, prospective cohort study collected data from patients undergoing common acute and elective general surgical, urological, gynaecological, and orthopaedic procedures. The primary outcomes were patient-reported time in severe pain measured on a numerical analogue scale from 0 to 100% and patient-reported satisfaction with pain relief during the first week following discharge. Data were collected by in-hospital chart review and patient telephone interview 1 week after discharge.Results: The study recruited 4273 patients from 144 centres in 25 countries; 1311 patients (30.7%) were prescribed opioid analgesia at discharge. Patients reported being in severe pain for 10 (i.q.r. 1-30)% of the first week after discharge and rated satisfaction with analgesia as 90 (i.q.r. 80-100) of 100. After adjustment for confounders, opioid analgesia on discharge was independently associated with increased pain severity (risk ratio 1.52, 95% c.i. 1.31 to 1.76; P < 0.001) and re-presentation to healthcare providers owing to side-effects of medication (OR 2.38, 95% c.i. 1.36 to 4.17; P = 0.004), but not with satisfaction with analgesia (beta coefficient 0.92, 95% c.i. -1.52 to 3.36; P = 0.468) compared with opioid-free analgesia. Although opioid prescribing varied greatly between high-income and low- and middle-income countries, patient-reported outcomes did not.Conclusion: Opioid analgesia prescription on surgical discharge is associated with a higher risk of re-presentation owing to side-effects of medication and increased patient-reported pain, but not with changes in patient-reported satisfaction. Opioid-free discharge analgesia should be adopted routinely

Onion Seed Germination as Affected by Temperature and Light

Producers need to know whether the germination rate will produce sufficient numbers of seedlings needed for field production of onion (Allium cepa L.). The influence of temperature (5.0, 7.5, 10, 15, 20, 25, 30, 35, or 40°C) and continuous light or continuous dark was tested for effects on percentage germination of normal, abnormal, or diseased seed; production of normal seedlings; and percentage of ungerminated solid and soft seeds of onion. Temperatures from 7.5 to 30°C generally assured a high germination percentage and a high percentage of normal seedlings. The most rapid germination occurred at 25°C in the dark. At 5, 35, and 40°C normal germination was only about 10%. The model from the Weibull function indicated that temperature influenced the time between the beginning of imbibition to germination. Onion seed exposed to temperatures between 10 and 30°C under field conditions could produce a high germination percentage, which should lead to development of normal seedlings

Interaction effects of phosphorus and zinc on photosynthesis, growth and yield of dwarf bean grown in two environments

Two experiments were conducted in a factorial combination of three Zn levels (0, 10 and 40 mg Zn kg-1 soil) and two P levels (0 and 200 mg P kg-1 soil). Experiment 1 was carried out during winter in a heated glasshouse, and experiment 2 during summer under a rain shelter. Plants of dwarf bean (Phaseolus vulgaris L., cv. Borlotto nano) were grown in pots filled with sandy soil. In both experiments, leaf Zn concentration was reduced by the addition of P to plants grown at low Zn supply. However, leaf Zn concentration lower than the critical level was observed only during experiment 2, and the main effects of low Zn were reductions of internode length, light use efficiency and maximum photosynthetic rate. In plants with leaf Zn concentration lower than the critical level, saturating irradiance levels fell from ~1000 μmol m-2 s-1 PPFD to ~300-400 μmol m-2 s-1 PPFD. Reduction of net photosynthesis was observed from the beginning of flowering and led to decreased seed production