Influence of nanoemulsion on the adhesion and survival of Aeromonas sp. in meat and contact surfaces of meat

In the present investigation, coconut oil–based nanoemulsion, designated as AUSN3, was evaluated for its influence on the adhesion and survival of Aeromonas sp. AUBAS34 to beef, pork, and mutton and contact surfaces of meat such as polystyrene, glass, and stainless steel. AUSN3 treatment reduced the hydrophobicity, motility, biofilm formation, and bacterial adhesion of AUBAS34 to meat and the contact surfaces. AUSN3 treatment completely eliminated the AUBAS34 population in the contact surfaces of meat within a time period of 20 min. In meat surfaces, AUSN3 treatment resulted in 1.3–1.8 log reduction in Aeromonas population compared to sodium nitrite treatment and 2.4–3.2 log reduction compared to control

Simultaneous Application of Biosurfactant and Bioaugmentation with Rhamnolipid-Producing Shewanella for Enhanced Bioremediation of Oil-Polluted Soil

In the present study, a combined treatment strategy involving the addition of rhamnolipid, rhamnolipid-producing bacteria (Shewanella sp. BS4) and a native soil microbial community for the remediation of hydrocarbon-contaminated soil under pilot-scale conditions was adopted. The isolate BS4 (rhl+), demonstrating the highest emulsification activity and surface tension reduction efficiency, was identified based on 16 S rDNA sequencing as Shewanella sp. strain. Growth conditions for rhamnolipid production were optimized based on Central Composite Design (CCD) as 2.9% crude oil, a 54 × 106 CFU g−1 inoculation load of soil, a temperature of 30.5 °C, and a pH of 6.7. In situ bioremediation experiments, conducted using hydrocarbon-contaminated soil treated with the combination of rhamnolipid and rhamnolipid-producing bacteria, showed that the inoculated Shewanella sp. BS4, along with the indigenous soil microbial community, supported the highest hydrocarbon-degrading bacterial population and soil respiration activity, and this treatment resulted in 75.8% hydrocarbon removal efficiency, which was higher compared to contaminated soil devoid of any treatment