AGO1 and AGO2 Act Redundantly in miR408-Mediated Plantacyanin Regulation

Background: In Arabidopsis, AGO1 and AGO2 associate with small RNAs that exhibit a Uridine and an Adenosine at their 59 end, respectively. Because most plant miRNAs have a 59U, AGO1 plays many essential roles in miRNA-mediated regulation of development and stress responses. In contrast, AGO2 has only been implicated in antibacterial defense in association with miR393*, which has a 59A. AGO2 also participates in antiviral defense in association with viral siRNAs. Principal Findings: This study reveals that miR408, which has a 59A, regulates its target Plantacyanin through either AGO1 or AGO2. Indeed, neither ago1 nor ago2 single mutations abolish miR408-mediated regulation of Plantacyanin. Only an ago1 ago2 double mutant appears compromised in miR408-mediated regulation of Plantacyanin, suggesting that AGO1 and AGO2 have redundant roles in this regulation. Moreover, the nature of the 59 nucleotide of miR408 does not appear essential for its regulatory role because both a wildtype 59A-MIR408 and a mutant 59U-MIR408 gene complement a mir408 mutant. Conclusions/Significance: These results suggest that miR408 associates with both AGO1 and AGO2 based on criteria that differ from the 59 end rule, reminiscent of miR390-AGO7 and miR165/166-AGO10 associations, which are not based on the nature of the 59 nucleotide

New genomics tools and resources. Dijon 2004 Highlights

Küster H, Bendahmane A. New genomics tools and resources. Dijon 2004 Highlights. Grain Legumes. 2004;40

Alteración en la expresión de la proteína DELLA u ortóloga para alterar el patrón de crecimiento de las plantas y el contenido de metabolitos del fruto

La presente invención describe nuevas funciones del gen SIDELLA de Solanum Iycopersicum así como sus usos y versiones mutantes del gen asociadas a nuevos fenotipos que suponen la modificación del hábito de crecimiento y del metabolismo de las plantas portadoras de esas alteraciones así como cierta influencia en el contenido de metabolitos del fruto del tomate.Consejo Superior de Investigaciones Científicas (España), Universidad Politécnica de ValenciaA1 Solicitud de patente con informe sobre el estado de la técnic

A mutation in the melon Vacuolar Protein Sorting 41prevents systemic infection of Cucumber mosaic virus

In the melon exotic accession PI 161375, the gene cmv1, confers recessive resistance to Cucumber mosaic virus (CMV) strains of subgroup II. cmv1 prevents the systemic infection by restricting the virus to the bundle sheath cells and impeding viral loading to the phloem. Here we report the fne mapping and cloning of cmv1. Screening of an F2 population reduced the cmv1 region to a 132 Kb interval that includes a Vacuolar Protein Sorting 41 gene. CmVPS41 is conserved among plants, animals and yeast and is required for post-Golgi vesicle trafcking towards the vacuole. We have validated CmVPS41 as the gene responsible for the resistance, both by generating CMV susceptible transgenic melon plants, expressing the susceptible allele in the resistant cultivar and by characterizing CmVPS41 TILLING mutants with reduced susceptibility to CMV. Finally, a core collection of 52 melon accessions allowed us to identify a single amino acid substitution (L348R) as the only polymorphism associated with the resistant phenotype. CmVPS41 is the frst natural recessive resistance gene found to be involved in viral transport and its cellular function suggests that CMV might use CmVPS41 for its own transport towards the phloem.info:eu-repo/semantics/publishedVersio

A mutation in the melon vacuolar protein sorting 41prevents systemic infection of cucumber mosaic virus

In the melon exotic accession PI 161375, the gene cmv1, confers recessive resistance to Cucumber mosaic virus (CMV) strains of subgroup II. cmv1 prevents the systemic infection by restricting the virus to the bundle sheath cells and impeding viral loading to the phloem. Here we report the fine mapping and cloning of cmv1. Screening of an F2 population reduced the cmv1 region to a 132 Kb interval that includes a Vacuolar Protein Sorting 41 gene. CmVPS41 is conserved among plants, animals and yeast and is required for post-Golgi vesicle trafficking towards the vacuole. We have validated CmVPS41 as the gene responsible for the resistance, both by generating CMV susceptible transgenic melon plants, expressing the susceptible allele in the resistant cultivar and by characterizing CmVPS41 TILLING mutants with reduced susceptibility to CMV. Finally, a core collection of 52 melon accessions allowed us to identify a single amino acid substitution (L348R) as the only polymorphism associated with the resistant phenotype. CmVPS41 is the first natural recessive resistance gene found to be involved in viral transport and its cellular function suggests that CMV might use CmVPS41 for its own transport towards the phloem

KARRIKIN INSENSITIVE2 regulates leaf development, root system architecture and arbuscular‐mycorrhizal symbiosis in Brachypodium distachyon

International audienceKarrikins, of which KAR1 and KAR2 are representatives, are a class of abiotic plant growth regulators produced from the partial combustion of plant material (Flematti et al., 2004; Flematti et al., 2009). Released into the soil following wildfires, karrikins can promote seed germination and seedling photomorphogenesis, and thereby facilitate the revegetation process (Nelson et al., 2009; Nelson et al., 2010; Stevens et al., 2007). The butenolide moiety of karrikins is also an essential structural feature of strigolactones (SLs), which are carotenoid-derived plant hormones that regulate a range of developmental processes in plants, and also serve as a rhizosphere signal for arbuscular mycorrhizal fungi and root parasitic weeds (Waters et al., 2016; Bürger and Chory, 2020). Collectively, these butenolides possess wide-ranging biological activities with considerable agricultural and environmental significance

KARRIKIN INSENSITIVE2 regulates leaf development, root system architecture and arbuscular‐mycorrhizal symbiosis in Brachypodium distachyon

International audienceKarrikins, of which KAR1 and KAR2 are representatives, are a class of abiotic plant growth regulators produced from the partial combustion of plant material (Flematti et al., 2004; Flematti et al., 2009). Released into the soil following wildfires, karrikins can promote seed germination and seedling photomorphogenesis, and thereby facilitate the revegetation process (Nelson et al., 2009; Nelson et al., 2010; Stevens et al., 2007). The butenolide moiety of karrikins is also an essential structural feature of strigolactones (SLs), which are carotenoid-derived plant hormones that regulate a range of developmental processes in plants, and also serve as a rhizosphere signal for arbuscular mycorrhizal fungi and root parasitic weeds (Waters et al., 2016; Bürger and Chory, 2020). Collectively, these butenolides possess wide-ranging biological activities with considerable agricultural and environmental significance