Bilateral enucleation alters gene expression and intraneocortical connections in the mouse

<p>Abstract</p> <p>Background</p> <p>Anatomically and functionally distinct sensory and motor neocortical areas form during mammalian development through a process called arealization. This process is believed to be reliant on both activity-dependent and activity-independent mechanisms. Although both mechanisms are thought to function concurrently during arealization, the nature of their interaction is not understood. To examine the potential interplay of extrinsic activity-dependent mechanisms, such as sensory input, and intrinsic activity-independent mechanisms, including gene expression in mouse neocortical development, we performed bilateral enucleations in newborn mice and conducted anatomical and molecular analyses 10 days later. In this study, by surgically removing the eyes of the newborn mouse, we examined whether early enucleation would impact normal gene expression and the development of basic anatomical features such as intraneocortical connections and cortical area boundaries in the first 10 days of life, before natural eye opening. We examined the acute effects of bilateral enucleation on the lateral geniculate nucleus of the thalamus and the neocortical somatosensory-visual area boundary through detailed analyses of intraneocortical connections and gene expression of six developmentally regulated genes at postnatal day 10.</p> <p>Results</p> <p>Our results demonstrate short-term plasticity on postnatal day 10 resulting from the removal of the eyes at birth, with changes in nuclear size and gene expression within the lateral geniculate nucleus as well as a shift in intraneocortical connections and <it>ephrin A5 </it>expression at the somatosensory-visual boundary. In this report, we highlight the correlation between positional shifts in <it>ephrin A5 </it>expression and improper refinement of intraneocortical connections observed at the somatosensory-visual boundary in enucleates on postnatal day 10.</p> <p>Conclusions</p> <p>Bilateral enucleation induces a positional shift of both <it>ephrin A5 </it>expression and intraneocortical projections at the somatosensory-visual border in only 10 days. These changes occur prior to natural eye opening, suggesting a possible role of spontaneous retinal activity in area border formation within the neocortex. Through these analyses, we gain a deeper understanding of how extrinsic activity-dependent mechanisms, particularly input from sensory organs, are integrated with intrinsic activity-independent mechanisms to regulate neocortical arealization and plasticity.</p

Molecular Dynamics Simulations of Liquid and Polymer Electrolytes for Energy Storage Devices

Advancing beyond current lithium-ion technology is necessary in order to enable energy storage devices for electric airplanes. Electrolyte stability is a key limiting factor, yet the design of improved electrolytes remains a formidable challenge. Molecular dynamics (MD) simulations are a powerful tool for studying electrolytes, since they can be used to evaluate structural, thermodynamic, and transport properties, and can provide molecular-level detail often inaccessible to experimental techniques. Our computational materials groups at the NASA Ames Research Center has developed models and methods to accurately simulate both liquid and polymer electrolytes.We report the results from atomistic MD simulations of several electrolyte materials, with lithium salts dissolved in ionic liquids, dimethoxyethane (DME), and polyethylene oxide (PEO). For improved accuracy, we employ polarizable models, where each atom is given an environment-dependent atomic dipole. The simulations accurately predict bulk transport properties, including viscosity, diffusion, and ionic conductivity, in quantitative agreement with available experimental data. Moreover, the simulations provide important insights into the solvation structure of the lithium ions.We also report the results from coarse-grained MD simulations of polyanion electrolytes. In order to more efficiently capture the longer length- and time-scales of these systems, we employ a generic bead-spring model. These simulations provide important insight into how the polymer chain architecture and ionic interaction strengths affect the ionic aggregation behavior and cation dynamics. Despite the simplicity of the model, the simulations yield qualitative agreement with experimental data for similar systems

Airborne Evaluation and Demonstration of a Time-Based Airborne Inter-Arrival Spacing Tool

An airborne tool has been developed that allows an aircraft to obtain a precise inter-arrival time-based spacing interval from the preceding aircraft. The Advanced Terminal Area Approach Spacing (ATAAS) tool uses Automatic Dependent Surveillance-Broadcast (ADS-B) data to compute speed commands for the ATAAS-equipped aircraft to obtain this inter-arrival spacing behind another aircraft. The tool was evaluated in an operational environment at the Chicago O'Hare International Airport and in the surrounding terminal area with three participating aircraft flying fixed route area navigation (RNAV) paths and vector scenarios. Both manual and autothrottle speed management were included in the scenarios to demonstrate the ability to use ATAAS with either method of speed management. The results on the overall delivery precision of the tool, based on a target spacing of 90 seconds, were a mean of 90.8 seconds with a standard deviation of 7.7 seconds. The results for the RNAV and vector cases were, respectively, M=89.3, SD=4.9 and M=91.7, SD=9.0

Three Numerical Puzzles and the Top Quark's Chiral Weak-Moment

Versus the standard model's t --> W b decay helicity amplitudes, three numerical puzzles occur at the 0.1 % level when one considers the amplitudes in the case of an additional (f_M + f_E) coupling of relative strength 53 GeV. The puzzles are theoretical ones which involve the t --> W b decay helicity amplitudes in the two cases, the relative strength of this additional coupling, and the observed masses of these three particles. A deeper analytic realization is obtained for two of them. Equivalent realizations are given for the remaining one. An empirical consequence of these analytic realizations is that it is important to search for effects of a large chiral weak-moment of the top-quark, the effective mass-scale is about 53 GeV. A full theoretical resolution would include relating the origin of such a chiral weak-moment and the mass generation of the top-quark, the W-boson, and probably the b-quark.Comment: 18 pages, 1 postscript table (revised to better explain notation, model #1, add a little material...

Reduction of the Three Dimensional Schrodinger Equation for Multilayered Films

In this paper, we present a method for reducing the three dimensional Schrodinger equation to study confined metallic states, such as quantum well states, in a multilayer film geometry. While discussing some approximations that are employed when dealing with the three dimensionality of the problem, we derive a one dimensional equation suitable for studying such states using an envelope function approach. Some applications to the Cu/Co multilayer system with regard to spin tunneling/rotations and angle resolved photoemission are discussed.Comment: 14 pages, 1 figur

Significance Testing of Archeological Site 41SR242, The Cornelio Alvarez Sr. Site, Starr County, Texas

The Texas Department of Transportation (TxDOT), conducted National Register of Historic Places (NRHP) eligibility testing of the Cornelio Alvarez Sr. site (41SR242) as part of the State Loop (SL) 195 project (Project) (CSJ: 3632-01-001) in Starr County, Texas. Subsequent to the field investigations, SWCA Environmental Consultants (SWCA) conducted artifact analysis, reporting, and curation preparation for the multi-component historic and prehistoric site. Investigations were conducted in compliance with Section 106 of the National Historic Preservation Act (54 United State Code 30601) and the Antiquities Code of Texas (9 Natural Resources Code). The investigations assessed the site’s eligibility for listing on the NRHP (36 Code of Federal Regulations 60.4) and for designation as a State Antiquities Landmark (SAL; 13 Texas Administrative Code 26.8, 26.12). Christopher W. Ringstaff served as Principal Investigator under Texas Antiquities Permit Number 7912. TxDOT conducted the field investigations were from February 20–24, 2017, and April 10–14, 2017. Site 41SR242 is primarily a Middle to Late Archaic site with lesser Late Prehistoric and perhaps earlier components. The open occupational site is located on an upland margin landform in a tributary valley a few miles from the Rio Grande. The investigations revealed material assemblages consisting of diffusely scattered burned rock, debitage, and lithic tools, which were predominantly recovered from a 30- to 50-cm-thick stratum of mixed artifacts. However, a few concentrations of artifacts were identified, and each location yielded isolated intact features. Formation and post-depositional processes are generally not conducive to preservation of intact archeological surfaces, patterns, or site structure. Although the overall site lacks integrity and potential data yield, isolated discrete behavioral loci are present. Therefore, site 41SR242 is recommended as eligible for the NRHP and as an SAL. This recommendation pertains to the portions of the site within the APE. The site extends beyond the APE, and the areas outside of the APE have not been evaluated

Significance Testing Of Archeological Sites 41SR242, The Cornelio Alvarez SR. Site, Starr County, Texas

The Texas Department of Transportation (TxDOT), conducted National Register of Historic Places (NRHP) eligibility testing of the Cornelio Alvarez Sr. site (41SR242) as part of the State Loop (SL) 195 project (Project) (CSJ: 3632-01-001) in Starr County, Texas. Subsequent to the field investigations, SWCA Environmental Consultants (SWCA) conducted artifact analysis, reporting, and curation preparation for the multi-component historic and prehistoric site. Investigations were conducted in compliance with Section 106 of the National Historic Preservation Act (54 United State Code 30601) and the Antiquities Code of Texas (9 Natural Resources Code). The investigations assessed the site’s eligibility for listing on the NRHP (36 Code of Federal Regulations 60.4) and for designation as a State Antiquities Landmark (SAL; 13 Texas Administrative Code 26.8, 26.12). Christopher W. Ringstaff served as Principal Investigator under Texas Antiquities Permit Number 7912. TxDOT conducted the field investigations were from February 20–24, 2017, and April 10–14, 2017. Site 41SR242 is primarily a Middle to Late Archaic site with lesser Late Prehistoric and perhaps earlier components. The open occupational site is located on an upland margin landform in a tributary valley a few miles from the Rio Grande. The investigations revealed material assemblages consisting of diffusely scattered burned rock, debitage, and lithic tools, which were predominantly recovered from a 30- to 50- cm-thick stratum of mixed artifacts. However, a few concentrations of artifacts were identified, and each location yielded isolated intact features. Formation and post-depositional processes are generally not conducive to preservation of intact archeological surfaces, patterns, or site structure. Although the overall site lacks integrity and potential data yield, isolated discrete behavioral loci are present. Therefore, site 41SR242 is recommended as eligible for the NRHP and as an SAL. This recommendation pertains to the portions of the site within the APE. The site extends beyond the APE, and the areas outside of the APE have not been evaluated

Flight validation of ground-based assessment for control power requirements at high angles of attack

A review is presented in viewgraph format of an ongoing NASA/U.S. Navy study to determine control power requirements at high angles of attack for the next generation high-performance aircraft. This paper focuses on recent flight test activities using the NASA High Alpha Research Vehicle (HARV), which are intended to validate results of previous ground-based simulation studies. The purpose of this study is discussed, and the overall program structure, approach, and objectives are described. Results from two areas of investigation are presented: (1) nose-down control power requirements and (2) lateral-directional control power requirements. Selected results which illustrate issues and challenges that are being addressed in the study are discussed including test methodology, comparisons between simulation and flight, and general lessons learned