Horizontal Branch Morphology and Mass Loss in Globular Clusters

The connection between mass loss on the red giant branch (RGB) and horizontal branch (HB) morphology in globular clusters (GCs) has long been acknowledged but the mechanisms governing mass loss remains poorly understood from a theoretical perspective. The present study uses synthetic HB models to demonstrate for the first time that alpha-enhancement and a simple relation between mass loss and metallicity can explain the entire range of HB morphology (characterized by the HB type index) observed in old, coeval GCs. The mass loss-metallicity relation accounts naturally for the fact that the most metal poor GCs ([Fe/H] < -2) have redder HBs than is typical of GCs with -2 < [Fe/H] < -1.5 without invoking younger ages. These results may prove useful in studying the contribution of HB stars to integrated light via stellar population synthesis.Comment: 13 pages, 5 figures, to appear in ApJ Letters (figure 2 may not display correctly in some PDF viewers

An HST/WFC3 view of stellar populations on the horizontal branch of NGC 2419

We use images acquired with the Hubble Space Telescope Wide Field Camera 3 and new models to probe the horizontal branch (HB) population of the Galactic globular cluster (GC) NGC 2419. A detailed analysis of the composite HB highlights three populations: (1) the blue luminous HB, hosting standard helium stars (Y = 0.25) with a very small spread of mass; (2) a small population of stars with intermediate helium content (0.26 < Y ≲ 0.29); and (3) the well-populated extreme HB. We can fit the last group with models having high helium abundance (Y ∼ 0.36), half of which (the hottest part, ‘blue hook’ stars) are identified as possible ‘late flash mixed stars’. The initial helium abundance of this extreme population is in nice agreement with the predicted helium abundance in the ejecta of massive asymptotic giant branch (AGB) stars of the same metallicity as NGC 2419. This result further supports the hypothesis that second-generation stars in GCs formed from the ashes of intermediate-mass AGB stars. We find that the distribution in magnitude of the blue hook stars is larger than that predicted by theoretical models. We discuss the possible uncertainties in the magnitude scales and different attempts to model this group of stars. Finally, we suggest that consistency can be better achieved if we assume core masses larger than predicted by our models. This may be possible if the progenitors were fast rotators on the main sequence. If further study confirms this interpretation, a fast initial rotation would be a strong signature of the peculiarity of extreme second-generation stars in GCs

Deep ACS Imaging in the Globular Cluster NGC 6397: The Cluster Color Magnitude Diagram and Luminosity Function

We present the CMD from deep HST imaging in the globular cluster NGC 6397. The ACS was used for 126 orbits to image a single field in two colors (F814W, F606W) 5 arcmin SE of the cluster center. The field observed overlaps that of archival WFPC2 data from 1994 and 1997 which were used to proper motion (PM) clean the data. Applying the PM corrections produces a remarkably clean CMD which reveals a number of features never seen before in a globular cluster CMD. In our field, the main sequence stars appeared to terminate close to the location in the CMD of the hydrogen-burning limit predicted by two independent sets of stellar evolution models. The faintest observed main sequence stars are about a magnitude fainter than the least luminous metal-poor field halo stars known, suggesting that the lowest luminosity halo stars still await discovery. At the bright end the data extend beyond the main sequence turnoff to well up the giant branch. A populous white dwarf cooling sequence is also seen in the cluster CMD. The most dramatic features of the cooling sequence are its turn to the blue at faint magnitudes as well as an apparent truncation near F814W = 28. The cluster luminosity and mass functions were derived, stretching from the turn off down to the hydrogen-burning limit. It was well modeled with either a very flat power-law or a lognormal function. In order to interpret these fits more fully we compared them with similar functions in the cluster core and with a full N-body model of NGC 6397 finding satisfactory agreement between the model predictions and the data. This exercise demonstrates the important role and the effect that dynamics has played in altering the cluster IMF.Comment: 43 pages including 4 tables and 12 diagrams. Figures 2 and 3 have been bitmapped. Accepted for publication in the Astronomical Journa

Multiple stellar populations in Magellanic Cloud clusters - VI. A survey of multiple sequences and Be stars in young clusters

The split main sequences (MSs) and extended MS turnoffs (eMSTOs) detected in a few young clusters have demonstrated that these stellar systems host multiple populations differing in a number of properties such as rotation and, possibly, age. We analyse Hubble Space Telescope photometry for 13 clusters with ages between ~40 and ~1000 Myr and of different masses. Our goal is to investigate for the first time the occurrence of multiple populations in a large sample of young clusters. We find that all the clusters exhibit the eMSTO phenomenon and that MS stars more massive than ~1.6M⊙ define a blue and a red MS, with the latter hosting the majority of MS stars. The comparison between the observations and isochrones suggests that the blue MSs are made of slow-rotating stars, while the red MSs host stars with rotational velocities close to the breakup value. About half of the bright MS stars in the youngest clusters are Ha emitters. These Be stars populate the red MS and the reddest part of the eMSTO, thus supporting the idea that the red MS is made of fast rotators. We conclude that the split MS and the eMSTO are a common feature of young clusters in both Magellanic Clouds. The phenomena of a split MS and an eMSTO occur for stars that are more massive than a specific threshold, which is independent of the host-cluster mass. As a by-product, we report the serendipitous discovery of a young Small Magellanic Cloud cluster, GALFOR 1

A Deep, Wide-Field, and Panchromatic View of 47 Tuc and the SMC with HST: Observations and Data Analysis Methods

In HST Cycle 17, we imaged the well known globular star cluster 47 Tucanae for 121 orbits using ACS and both the UVIS and IR channels of WFC3 (GO-11677, PI - H. Richer). This unique data set was obtained to address many scientific questions that demand a very deep, panchromatic, and panoramic view of the cluster's stellar populations. In total, the program obtained over 0.75 Ms of imaging exposure time with the three HST cameras, over a time span of 9 months in 2010. The primary ACS field was imaged in the two broadband filters F606W and F814W filters, at 13 orientations, for all 121 orbits. The parallel WFC3 imaging provides a panchromatic (0.4 - 1.7 micron) and contiguous imaging swath over a 250 degree azimuthal range at impact radii of 6.5 -- 17.9 pc in 47 Tuc. This imaging totals over 60 arcmin^2 in area and utilizes the F390W and F606W broadband filters on WFC3/UVIS and the F110W and F160W broadband filters on WFC3/IR. In this paper, we describe the observational design of the new survey and one of the methods used to analyze all of the imaging data. This analysis combines over 700 full-frame images taken with the three HST cameras into a handful of ultra-deep, well-sampled combined images in each of the six filters. The results reveal unprecedented color-magnitude diagrams (CMDs) of the cluster extending to >30th magnitude in the optical, 29th magnitude in the UV, and 27th magnitude in the IR. The data set provides a characterization of the complete stellar populations of 47 Tuc, extending from the faintest hydrogen burning dwarfs through the main-sequence and giant branches, down to very cool white dwarf remnants in the cluster. The imaging also provides the deepest probe of the stellar populations of the background Small Magellanic Cloud (SMC) galaxy, resolving low mass main-sequence dwarfs with M < 0.2 Msun.Comment: Accepted for Publication in the Astronomical Journal. 23 pages, 13 figures, and 4 tables. Companion paper by K. Woodley is astro-ph/037477

Modeling and mitigation of high-concentration antibody viscosity through structure-based computer-aided protein design.

For an antibody to be a successful therapeutic many competing factors require optimization, including binding affinity, biophysical characteristics, and immunogenicity risk. Additional constraints may arise from the need to formulate antibodies at high concentrations (>150 mg/ml) to enable subcutaneous dosing with reasonable volume (ideally <1.0 mL). Unfortunately, antibodies at high concentrations may exhibit high viscosities that place impractical constraints (such as multiple injections or large needle diameters) on delivery and impede efficient manufacturing. Here we describe the optimization of an anti-PDGF-BB antibody to reduce viscosity, enabling an increase in the formulated concentration from 80 mg/ml to greater than 160 mg/ml, while maintaining the binding affinity. We performed two rounds of structure guided rational design to optimize the surface electrostatic properties. Analysis of this set demonstrated that a net-positive charge change, and disruption of negative charge patches were associated with decreased viscosity, but the effect was greatly dependent on the local surface environment. Our work here provides a comprehensive study exploring a wide sampling of charge-changes in the Fv and CDR regions along with targeting multiple negative charge patches. In total, we generated viscosity measurements for 40 unique antibody variants with full sequence information which provides a significantly larger and more complete dataset than has previously been reported