FasL Expression in Articular Discs of Human Temporomandibular Joint and Association with Osteoarthrosis

Background Apoptosis is a programme of cell death which does not induce an inflammatory response. Recent previous research has suggested a correlation between temporomandibular internal derangement and apoptosis. Fas ligand (FasL) is an apoptosis‐inducing factor, known to trigger apoptosis through distinct signal pathways. This study aims to examine, by immunohistochemistry, the expression of FasL in temporomandibular joint (TMJ) articular discs of patients with anterior disc displacement with reduction (ADDwR) and without reduction (ADDwoR) in patients with and without osteoarthrosis (OA). Methods Forty‐two (n = 42) TMJ articular discs were divided into two cut‐offs: (i) 8 control, 17 ADDwR, 17 ADDwoR, and (ii) without OA (n = 25) and with OA (n = 17). The area of immunostaining was compared statistically between groups (P \u3c 0.05). Results Statistically significant differences were found in the expression of FasL in TMJ discs between the three groups (P = 0.001). ADDwR presented significant higher FasL expression when compared with ADDwoR (P \u3c 0.001). Significant higher FasL expression was observed in the group without OA (P = 0.001). All patients without OA presented ADDwR, while all the patients with OA presented ADDwoR. Conclusion A higher area of in situ immunostaining of FasL was found in temporomandibular discs with reduction, which is the less severe condition. Moreover, a reduced expression of FasL in the discs of patients with osteoarthrosis was found, suggesting that some aspects of apoptosis might underlie the progression of TMJ disorders

Simulation and evaluation of deep learning autoencoders for image compression in multi-UAV network systems

Mobile multi-robot systems are versatile alternatives for improving single-robot capacities in many applications, such as logistics, environmental monitoring, search and rescue, photogrammetry, etc. In this sense, this kind of system must have a reliable communication network between the vehicles, ensuring that information exchanged within the nodes has little losses. This work simulates and evaluates the use of autoencoders for image compression in a multi-UAV simulation with ROS and Gazebo for a generic surveillance application. The autoencoder model was developed with the Keras library, presenting good training and validation results, with training and validation accuracy of 70%, and a Peak Signal Noise Ratio (PSNR) of 40dB. The use of the CPU for the simulated UAVs for processing and sending compressed images through the network is 25% faster. The results showed that this compression methodology is a good choice for improving the system’s performance without losing too much information.The authors thank CEFET/RJ, UFF, UFRJ, and the Brazilian research agencies CAPES, CNPq, and FAPERJ. Besides, the authors are grateful to the Foundation for Science and Technology (FCT, Portugal) for financial support through national funds FCT/MCTES (PIDDAC) to CeDRI (UIDB/05757/2020 and UIDP/05757/2020) and SusTEC (LA/P/0007/2021).info:eu-repo/semantics/publishedVersio

An in vitro dynamic model of catheter-associated urinary tract infections to investigate the role of uncommon bacteria on the Escherichia coli microbial consortium

About 9% of nosocomial infections are attributed to catheter-associated urinary tract infections (CAUTIs). Uncommon bacteria (Delftia tusurhatensis) have been isolated in CAUTIs in combination with wellestablished pathogenic bacteria such as E. coli. Nonetheless, the reason why E. coli coexists with other bacteria instead of outcompeting and completely eliminating them are unknown. As such, a flow cell reactor simulating the hydrodynamic conditions found in CAUTIs (shear rate of 15 s-1) was used to characterize the microbial physiology of E. coli and D. tsuruhatensis individually and in consortium, in terms of growth kinetics and substrate uptake. Single-species biofilms showed that up to 48 h the CFU counts significantly increased for both species (p<0.05). After 48 h, both species stabilized with similar CFU values reaching log 6.24 CFU.cm2 for E. coli and log 6.31 CFU.cm2 for D. tsuruhatensis (p>0.05). The assessment of spatial distribution of dual-species biofilms by LNA/2´OMe-FISH revealed that E. coli and D. tsuruhatensis coexist and tend to co-aggregate over time, which implies that bacteria are able to cooperate synergistically. Substrate uptake measurements revealed that in artificial urine medium the bacteria metabolized lactic acid, uric acid (E. coli and D. tsuruhatensis) and citric acid (D. tsuruhatensis). In the consortium, D. tsuruhatensis consumed citric acid more rapidly, presumably leaving more uric acid available in the medium to be used by E. coli. In conclusion, metabolic cooperation between E. coli and uncommon species seems to occur when these species share the same environment, leading to the formation of a stable microbial community

Incidência de infecção em cateteres venosos centrais e fatores de risco em um hospital universitário

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ESTUDO DO POTENCIAL DE INDICAÇÃO GEOGRÁFICA DO DOCE DE LEITE BRANCO DE AFRÂNIO - PE - BRASIL

As Indicações Geográficas (IG) são uma ferramenta de valorização da biodiversidade, do conhecimento regional e dos recursos naturais, e podem oferecer contribuições extremamente positivas para as economias locais e o dinamismo de cada região. Quando um produto ou alimento possui em seu nome referência à zona de produção e cujas características de qualidade decorrem dos atributos desse território e do saber fazer (tradição) dos produtores, pode-se considerar que esta tipicidade única é um bem imaterial, se tornando propriedade coletiva inseparável do território de produção. O principal objetivo deste trabalho, portanto, foi realizar um levantamento histórico a respeito da produção do doce de leite branco no município de Afrânio/PE. O estudo histórico realizado revelou que há mais de 70 anos o doce de leite branco é produzido de forma artesanal no município de Afrânio e, desde então, sua qualidade é reconhecida por toda região, comprovada por depoimentos orais e periódicos locais