Detecção Do Vírus Da Cinomose Canina Por Diferentes Métodos De One-step Rt-qpcr

Three commercial kits of One-Step RT-qPCR were evaluated for the molecular diagnosis of Canine Distemper Virus. Using the kit that showed better performance, two systems of Real-time RT-PCR (RT-qPCR) assays were tested and compared for analytical sensitivity to Canine Distemper Virus RNA detection: a One-Step RT-qPCR (system A) and a One-Step RT-qPCR combined with NESTED-qPCR (system B). Limits of detection for both systems were determined using a serial dilution of Canine Distemper Virus synthetic RNA or a positive urine sample. In addition, the same urine sample was tested using samples with prior centrifugation or ultracentrifugation. Commercial kits of One-Step RT-qPCR assays detected canine distemper virus RNA in 10 (100%) urine samples from symptomatic animals tested. The One-Step RT-qPCR kit that showed better results was used to evaluate the analytical sensitivity of the A and B systems. Limit of detection using synthetic RNA for the system A was 11 RNA copies µL−1 and 110 RNA copies µl−1 for first round System B. The second round of the NESTED-qPCR for System B had a limit of detection of 11 copies µl−1. Relationship between Ct values and RNA concentration was linear. The RNA extracted from the urine dilutions was detected in dilutions of 10−3 and 10−2 by System A and B respectively. Urine centrifugation increased the analytical sensitivity of the test and proved to be useful for routine diagnostics. The One-Step RT-qPCR is a fast, sensitive and specific method for canine distemper routine diagnosis and research projects that require sensitive and quantitative methodology. © 2016, Universidade Federal de Santa Maria. All rights reserved.4691601160

Analysis of irregular three-dimensional packing problems in additive manufacturing: a new taxonomy and dataset

© 2018 Informa UK Limited, trading as Taylor & Francis Group. With most Additive Manufacturing (AM) technology variants, build processes take place inside an internal enclosed build container, referred to as a ‘build volume’. It has been demonstrated that the effectiveness with which this volume is filled with product geometries forms an important determinant of overall process efficiency in AM. For effective operations management, it is important to understand not only the problem faced, but also which methods have proved effective (or ineffective) for problems with these characteristics in the past. This research aims to facilitate this increased understanding. The build volume packing task can be formulated as a three-dimensional irregular packing (3DIP) problem, which is a combinatorial optimisation problem requiring the configuration of a set of arbitrary volumetric items. This paper reviews existing general cutting and packing taxonomies and provides a new specification which is more appropriate for classifying the problems encountered in AM. This comprises a clear-cut problem definition, a set of precise categorisation criteria for objectives and problem instances, and a simple notation. Furthermore, the paper establishes an improved terminology with terms that are familiar to, but not limited to, researchers and practitioners in the field of AM. Finally, this paper describes a new dataset to be used in the evaluation of existing and proposed computational solution methods for 3DIP problems encountered in AM and discusses the importance of this research for further underpinning work

Enrelvamento em vinha de encosta não regada: 2 - efeitos no crescimento vegetativo, produção e qualidade do mosto e vinho, casta Cabernet Sauvignon

Grapevine vegetative growth, yield, fruit composition and wine quality were studied in the Estremadura Winegrowing Region of Portugal in a ‘Cabernet Sauvignon’ slopping non-irrigated vineyard. During three seasons three treatments were compared: soil tillage (control), permanent resident vegetation, and permanent sown cover crop. When compared to soil tillage, the inter-row sward treatments displayed a lower predawn leaf water potential from bloom to mid-ripening. These differences in vine water status did not affect vine yield or berry sugar accumulation; however, in the third season after experiment setup it induced a significant reduction in vegetative growth in the sward treatments, compared to soil tillage. This vegetative growth reduction had a positive effect on grape composition by reducing titratable acidity and increasing berry skin total phenols and anthocyanins. Those differences were also detected in the wines by the judges who gave a better classification to the wines from the sward treatments. Our results indicate that cover cropping can be a valuable tool for controlling vigour and enhancing wine quality in this winegrowing region

Enrelvamento em vinha de encosta não regada: 1 - efeito na composição e dinâmica das infestantes

The influence of two sward treatments and soil cultivation on the composition, the structure, and the evolution of the biomass of vineyard weed communities was examined. The 3-year study (2002-2004) was carried out in a sloping, non-irrigated vineyard, cv. ‘Cabernet Sauvignon’, in the Estremadura winegrowing region of Portugal. The experimental treatments were: soil tillage (control); permanent sown cover crop - Lolium perenne ‘Nui’, L. multiflorum ‘Bartíssimo’, Festuca ovina ‘Ridu’, F. rubra ssp. rubra ‘Echo’, Trifolium incarnatum. ‘Red’, T. repens ‘Huie’ and T. subterraneum ‘Claire’; and permanent resident vegetation. Total weed biomass in the spring did not reveal significant differences between treatments, but varied annually. The management practices – e.g. time and number of soil cultivations and inter-row mowing – were determinant in weed biomass evolution. Canonical correspondence analysis revealed significant treatment effects on community structure. Three years after the experiment was set up, in the soil tillage treatment weed composition was dominated by annual broad-leaved species, namely five Geraniaceae species, Medicago polymorpha and Sonchus oleraceus. The perennial broad-leaved species Oxalis pes-caprae was also a dominant species in soil tillage. In both sward treatments there was an increase in the perennial broad-leaved and grass species. Compared to soil tillage, in the resident vegetation treatment there was a significant increase in perennial species, such as Rumex crispus, Veronica anagallis-aquatica and Polypogon monspeliensis, and in the annuals Melilotus indica and Avena sterilis. The increase in these perennial species, which are considered to compete with vines, requires more frequent mowing in the summer. In the permanent sown cover crop treatment, L. perenne and T. repens displayed the ability to re-establish successfully, and their abundance decreased or suppressed most of the annual and perennial weed species

Influencia de la densidad animal en la respuesta productiva de conejos en una explotación industrial

publishedTomo I . Sección: Sistemas Ganaderos-Economía y Gestión. Sesión: Conejos, abejas, peces y cerdos. Ponencia nº 2

Extensive beef cattle production in Portugal

Portugal, located in the Mediterranean region, one of the "Hot Spot" of the planet, has a huge variability in terms of topography, soils, climate, agriculture, traditions, resulting in a high diversity of animal genetic resources, represented by 15 native cattle breeds, most of them threatened. A significant percentage of extensive beef cattle production in Portugal is based on the use of animals, differing from north to south region, with good maternal aptitude, hardiness, a low growth performance and exceptional adaptation to the environment where they live, and especially to the local feed, naturally produced. In the north of the country and the Azores, dairy breeds predominate, while in the south beef breeds are most prevalent. Portugal is not self-sufficient in beef production, however it is possible to appreciate an market for beef produced by indigenous breeds from extensive and organic production systems. Beef from indigenous breeds is considered to be a high quality product, mainly because of the superior taste and structure of the meat resulting from the production system: slow growth rate and type of vegetation consumed. This product differentiation has allowed the enlargement of small niche markets and has led to an increase in the value of meat with a Protected Designation of Origin (PDO) or with organic beef. Since portuguese agriculture cannot compete on quantity or production cost with other competitors, differentiation and quality seem to be the alternatives that may stimulate rural activities in Less Favoured Areas (LFAs) and create a regional added value able to contribute to sustainable development. Extensive animal production systems can be an important component of environmental and landscape protection, as well as contribute to the decrease of the human, physical desertification of our rural areas and animal genetic resources

Indicadores del alimentador automático en terneros de la raza Holstein Frisian en la región de entre Douro y Minho de Portugal

publishedTomo I . Sección: Sistemas Ganaderos-Economía y Gestión. Sesión: Producción de leche. Ponencia nº 3

Estudio del crecimiento de terneros con alimentador automático en la raza Holstein Frisia en la región de entre Douro y Minho de Portugal

publishedTomo I . Sección: Sistemas Ganaderos-Economía y Gestión. Sesión: Producción de leche. Ponencia nº 3

Automation and control of the SORTEGEL wastewater plant

Food Processing Industries produce large amounts of wastewater with high environmental impact. Due to the high content of suspended matter and inadequate pH value of the wastewater, national laws prohibit direct discharges of the influent to the environment. This work describes the design and operation of a wastewater treatment plant installed in the Sortegel food-processing company located in Sortes, Portugal. This industry uses the water collected from groundwater wells to process raw materials and to wash the equipments, being the volume of wastewater produced season dependent (80 to 300m3/day). Results show that the implemented wastewater treatment plant and the automation solutions generate treated effluents that comply with the Portuguese legislation

Evaluación de reservas corporales de grasa de vacas lecheras a través de un sistema de análisis de imagen

publishedTomo I . Sección: Sistemas Ganaderos-Economía y Gestión. Sesión: Producción de leche. Ponencia nº 3