Clinical and biological profiles of prospective blood donors at the Yaoundé Central Hospital and the Yaoundé University Teaching Hospital: Profils cliniques et biologiques des futurs donneurs de sang à l'hôpital central de Yaoundé et au CHU de Yaoundé

Assessing the profiles of prospective donors could help develop strategies to improve blood safety in our context. This study aimed to evaluate the clinical and biological profiles of prospective Cameroonian blood donors. To attain this objective, we carried out a cross-sectional and analytical study on all prospective blood donors that presented at the Yaoundé Central Hospital (HCY) and Yaoundé University Teaching Hospital (YUTH) blood banks from January 2017 to May 2017. After obtaining participants’ consent, we collected information from them, examined them, and collected five-milliliter blood samples from each of them. These blood samples were used to test for HIV, HBV, HCV, and Syphilis. A total of 247 prospective donors were retained for the study. We found that group “O” was noted in 50.9% of the participants and 97% were Rhesus-positive. Of the four main transfusion transmissible infections (TTIs) screened, 57 participants tested positive for at least one; nine were tested positive for HIV, twenty-six for HBV, ten for HCV, and sixteen for Syphilis. Co-infection was noted in four of the participants with two of them testing positive for both HIV and HBV, one for both HIV and HCV, and one for both HCV and Syphilis. On univariate analysis, a significant association was found between lymph nodes measuring more than 2 cm and the presence of a TTI. The presence of lymph nodes measuring more than 2 cm was associated with the presence of a TTI. L'évaluation des profils des donneurs potentiels pourrait aider à développer des stratégies pour améliorer la sécurité du sang dans notre contexte. Cette étude visait à évaluer les profils cliniques et biologiques des candidats donneurs de sang camerounais. Pour atteindre cet objectif, nous avons mené une étude transversale et analytique sur tous les candidats donneurs de sang qui se sont présentés aux banques de sang du Centre Hospitalier Central de Yaoundé (HCY) et du CHU de Yaoundé (YUTH) de janvier 2017 à mai 2017. Après obtention des participants ' consentement, nous avons recueilli des informations auprès d'eux, les avons examinés et prélevé des échantillons de sang de cinq millilitres sur chacun d'eux. Ces échantillons de sang ont été utilisés pour tester le VIH, le VHB, le VHC et la syphilis. Au total, 247 donneurs potentiels ont été retenus pour l'étude. Nous avons constaté que le groupe « O » était noté chez 50,9 % des participants et 97 % étaient rhésus positifs. Sur les quatre principales infections transmissibles par transfusion (ITT) dépistées, 57 participants ont été testés positifs pour au moins une ; neuf ont été testés positifs pour le VIH, vingt-six pour le VHB, dix pour le VHC et seize pour la syphilis. Une co-infection a été notée chez quatre des participants, dont deux étaient positifs à la fois pour le VIH et le VHB, un pour le VIH et le VHC et un pour le VHC et la syphilis. En analyse univariée, une association significative a été retrouvée entre les ganglions mesurant plus de 2 cm et la présence d'un ITT. La présence de ganglions mesurant plus de 2 cm était associée à la présence d'un ITT

Intensive tool-practice and skillfulness facilitate the extension of body representations in humans

The brain's representation of the body can be extended to include objects that are not originally part of the body. Various studies have found both extremely rapid extensions that occur as soon as an object is held, as well as extremely slow extensions that require weeks of training. Due to species and methodological differences, it is unclear whether the studies were probing different representations, or revealing multiple aspects of the same representation. Here, we present evidence that objects (cotton balls) held by a tool (chopsticks) are rapidly integrated into the body representation, as indexed by fading of the cotton balls (or ‘second-order extensions׳) from a positive afterimage. Skillfulness with chopsticks was predictive of more rapid integration of the second-order objects held by this tool. We also found that extensive training over a period of weeks augmented the level of integration. Together, our findings demonstrate integration of second-order objects held by tools, and reveal that the body representation probed by positive afterimages is subject to both rapid and slow processes of adaptive change

A Fast Modular Semiconductor-Based Marx Generator for Driving Dynamic Loads

The challenging demands of pulsed electron beam devices (such as the GESA device) with respect to their pulsed power supply have led to the development of a new semiconductor-based Marx generator. At a maximum output voltage of 120 kV and 600-A pulse current for a duration of up to 100 μS, stepwise arbitrary output waveforms are desired. A fast rise time of the generator is achieved by using fast switching circuitry, low inductance capacitors, and a low inductance stage arrangement. For low jitter triggering of all stages and efficient signal transmission, the generator uses an optical bus system for communication. Due to the inherent dynamic load characteristics of the GESA device, the generator features a fast overcurrent protection scheme. This paper presents selected design aspects of the generator and their validation in a small-scale assembly able of delivering up to 8 kV at 600-A load current

Alpha-band rhythms in visual task performance: phase-locking by rhythmic sensory stimulation

Oscillations are an important aspect of neuronal activity. Interestingly, oscillatory patterns are also observed in behaviour, such as in visual performance measures after the presentation of a brief sensory event in the visual or another modality. These oscillations in visual performance cycle at the typical frequencies of brain rhythms, suggesting that perception may be closely linked to brain oscillations. We here investigated this link for a prominent rhythm of the visual system (the alpha-rhythm, 8-12 Hz) by applying rhythmic visual stimulation at alpha-frequency (10.6 Hz), known to lead to a resonance response in visual areas, and testing its effects on subsequent visual target discrimination. Our data show that rhythmic visual stimulation at 10.6 Hz: 1) has specific behavioral consequences, relative to stimulation at control frequencies (3.9 Hz, 7.1 Hz, 14.2 Hz), and 2) leads to alpha-band oscillations in visual performance measures, that 3) correlate in precise frequency across individuals with resting alpha-rhythms recorded over parieto-occipital areas. The most parsimonious explanation for these three findings is entrainment (phase-locking) of ongoing perceptually relevant alpha-band brain oscillations by rhythmic sensory events. These findings are in line with occipital alpha-oscillations underlying periodicity in visual performance, and suggest that rhythmic stimulation at frequencies of intrinsic brain-rhythms can be used to reveal influences of these rhythms on task performance to study their functional roles

Renewable Energy (RE): Bioenergy -Feedstocks and Pretreatment-

The Department for Pulsed Power Technology is focusing on research and development of pulsed power technologies and related applications. The applications involves the electroporation of biological cells for extraction of cell contents (PEF- process), dewatering and drying of green biomass, pre-treatment of micro algae for energetic use and sustainable reduction of bacteria in contaminated effluents. Another key research topic is devoted to the development of corrosion barriers and materials for improved compatibility of structural materials in contact with liquid metal coolants. This year\u27s report focuses primarily on the activities and results of ongoing third-party funded projects of the department

Assessment of Arthrobacter viscosus as reactive medium for forming permeable reactive biobarrier applied to PAHs remediation

Polycyclic aromatic hydrocarbons (PAHs) are significant environmental contaminants as they are present naturally as well as anthropogenically in soil, air and water. In spite of their low solubility, PAHs are spread to the environment, and they are present in surface water, industrial effluent or groundwater. Amongst all remediation technologies for treating groundwater contaminated with PAHs, the use of a permeable reactive biobarrier (PRBB) appears to be the most cost-effective, energy efficient, and environmentally sound approach. In this technology, the microorganisms are used as reactive medium to degrade or stabilize the contaminants. The main limits of this approach are that the microorganisms or consortium used for forming the PRBB should show adequate characteristics. They must be retained in the barrier-forming biofilm, and they should also have degradative ability for the target pollutants. The aim of the present work is to evaluate the viability of Arthrobacter viscosus as bioreactive medium for forming PRBB. Initially, the ability of A. viscosus to remove PAHs, benzo[a]anthracene 100 μM and phenanthrene 100 μM was evaluated operating in a batch bench-scale bioreactor. In both cases, total benzo[a]anthracene and phenanthrene removals were obtained after 7 and 3 days, respectively. Furthermore, the viability of the microorganisms was evaluated in the presence of chromium in a continuous mode. As a final point, the adhesion of A. viscosus to sepiolite forming a bioreactive material to build PRBB was demonstrated. In view of the attained results, it can be concluded that A. viscosus could be a suitable microorganism to form a bioreactive medium for PAHs remediation.This work has been supported by the Spanish Ministry of Economy and Competitiveness and FEDER Funds (Project CTM 2011-25389). Marta Pazos received financial support under the Ramon y Cajal programme and Marta Cobas under the final project master grant "Campus do Mar Knowledge in depth"

TRY plant trait database - enhanced coverage and open access

Plant traits-the morphological, anatomical, physiological, biochemical and phenological characteristics of plants-determine how plants respond to environmental factors, affect other trophic levels, and influence ecosystem properties and their benefits and detriments to people. Plant trait data thus represent the basis for a vast area of research spanning from evolutionary biology, community and functional ecology, to biodiversity conservation, ecosystem and landscape management, restoration, biogeography and earth system modelling. Since its foundation in 2007, the TRY database of plant traits has grown continuously. It now provides unprecedented data coverage under an open access data policy and is the main plant trait database used by the research community worldwide. Increasingly, the TRY database also supports new frontiers of trait-based plant research, including the identification of data gaps and the subsequent mobilization or measurement of new data. To support this development, in this article we evaluate the extent of the trait data compiled in TRY and analyse emerging patterns of data coverage and representativeness. Best species coverage is achieved for categorical traits-almost complete coverage for 'plant growth form'. However, most traits relevant for ecology and vegetation modelling are characterized by continuous intraspecific variation and trait-environmental relationships. These traits have to be measured on individual plants in their respective environment. Despite unprecedented data coverage, we observe a humbling lack of completeness and representativeness of these continuous traits in many aspects. We, therefore, conclude that reducing data gaps and biases in the TRY database remains a key challenge and requires a coordinated approach to data mobilization and trait measurements. This can only be achieved in collaboration with other initiatives

Analysis of gene mutations associated with isoniazid, rifampicin and ethambutol resistance among Mycobacterium tuberculosis isolates from Ethiopia

<p>Abstract</p> <p>Background</p> <p>The emergence of drug resistance is one of the most important threats to tuberculosis control programs. This study was aimed to analyze the frequency of gene mutations associated with resistance to isoniazid (INH), rifampicin (RMP) and ethambutol (EMB) among <it>Mycobacterium tuberculosis </it>isolates from Northwest Ethiopia, and to assess the performance of the GenoType^®MTBDRplus and GenoType^®MTBDRsl assays as compared to the BacT/ALERT 3D system.</p> <p>Methods</p> <p>Two hundred sixty <it>Mycobacterium tuberculosis </it>isolates from smear positive tuberculosis patients diagnosed between March 2009 and July 2009 were included in this study. Drug susceptibility tests were performed in the Institute of Medical Microbiology and Epidemiology of Infectious Diseases, University Hospital of Leipzig, Germany.</p> <p>Results</p> <p>Of 260 isolates, mutations conferring resistance to INH, RMP, or EMB were detected in 35, 15, and 8 isolates, respectively, while multidrug resistance (MDR) was present in 13 of the isolates. Of 35 INH resistant strains, 33 had mutations in the <it>katG </it>gene at Ser315Thr 1 and two strains had mutation in the <it>inhA </it>gene at C15T. Among 15 RMP resistant isolates, 11 had <it>rpoB </it>gene mutation at Ser531Leu, one at His526Asp, and three strains had mutations only at the wild type probes. Of 8 EMB resistant strains, two had mutations in the <it>embB </it>gene at Met306Ile, one at Met306Val, and five strains had mutations only at the wild type probes. The GenoType^®MTBDRplus assay had a sensitivity of 92% and specificity of 99% for INH resistance, and 100% sensitivity and specificity to detect RMP resistance and MDR. The GenoType^®MTBDRsl assay had a sensitivity of 42% and specificity of 100% for EMB resistance.</p> <p>Conclusion</p> <p>The dominance of single gene mutations associated with the resistance to INH and RMP was observed in the codon 315 of the <it>katG </it>gene and codon 531 of the <it>rpoB </it>gene, respectively. The GenoType^®MTBDRplus assay is a sensitive and specific tool for diagnosis of resistance to INH, RMP and MDR. However, the GenoType^®MTBDRsl assay shows limitations in detecting resistance to EMB.</p

Plasticity of the Intrinsic Period of the Human Circadian Timing System

Human expeditions to Mars will require adaptation to the 24.65-h Martian solar day-night cycle (sol), which is outside the range of entrainment of the human circadian pacemaker under lighting intensities to which astronauts are typically exposed. Failure to entrain the circadian time-keeping system to the desired rest-activity cycle disturbs sleep and impairs cognitive function. Furthermore, differences between the intrinsic circadian period and Earth's 24-h light-dark cycle underlie human circadian rhythm sleep disorders, such as advanced sleep phase disorder and non-24-hour sleep-wake disorders. Therefore, first, we tested whether exposure to a model-based lighting regimen would entrain the human circadian pacemaker at a normal phase angle to the 24.65-h Martian sol and to the 23.5-h day length often required of astronauts during short duration space exploration. Second, we tested here whether such prior entrainment to non-24-h light-dark cycles would lead to subsequent modification of the intrinsic period of the human circadian timing system. Here we show that exposure to moderately bright light (∼450 lux; ∼1.2 W/m2) for the second or first half of the scheduled wake episode is effective for entraining individuals to the 24.65-h Martian sol and a 23.5-h day length, respectively. Estimations of the circadian periods of plasma melatonin, plasma cortisol, and core body temperature rhythms collected under forced desynchrony protocols revealed that the intrinsic circadian period of the human circadian pacemaker was significantly longer following entrainment to the Martian sol as compared to following entrainment to the 23.5-h day. The latter finding of after-effects of entrainment reveals for the first time plasticity of the period of the human circadian timing system. Both findings have important implications for the treatment of circadian rhythm sleep disorders and human space exploration