Tools and Procedures for the CTA Array Calibration

The Cherenkov Telescope Array (CTA) is an international initiative to build the next generation ground-based very-high-energy gamma-ray observatory. Full sky coverage will be assured by two arrays, one located on each of the northern and southern hemispheres. Three different sizes of telescopes will cover a wide energy range from tens of GeV up to hundreds of TeV. These telescopes, of which prototypes are currently under construction or completion, will have different mirror sizes and fields-of-view designed to access different energy regimes. Additionally, there will be groups of telescopes with different optics system, camera and electronics design. Given this diversity of instruments, an overall coherent calibration of the full array is a challenging task. Moreover, the CTA requirements on calibration accuracy are much more stringent than those achieved with current Imaging Atmospheric Cherenkov Telescopes, like for instance: the systematic errors in the energy scale must not exceed 10%.In this contribution we present both the methods that, applied directly to the acquired observational CTA data, will ensure that the calibration is correctly performed to the stringent required precision, and the calibration equipment that, external to the telescopes, is currently under development and testing. Moreover, some notes about the operative procedure to be followed with both methods and instruments, will be described. The methods applied to the observational CTA data include the analysis of muon ring images, of carefully selected cosmic-ray air shower images, of the reconstructed electron spectrum and that of known gamma-ray sources and the possible use of stereo techniques hardware-independent. These methods will be complemented with the use of calibrated light sources located on ground or on board unmanned aerial vehicles.Comment: All CTA contributions at arXiv:1709.0348

Single dose pharmacodynamics of amphotericin B against Aspergillus species in an in vitro pharmacokinetic/pharmacodynamic model

Conventional MIC testing of amphotericin B results in narrow MIC ranges challenging the detection of resistant strains. In order to discern amphotericin B pharmacodynamics, the in vitro activity of amphotericin B was studied against Aspergillus isolates with the same MIC with a new in vitro pharmacokinetic/pharmacodynamic (PK/PD) model that simulates amphotericin B human plasma levels. Clinical isolates of A. fumigatus, A. terreus and A flavus with the same CLSI modal MICs of 1 mg/l were exposed to amphotericin B concentrations following the plasma concentration-time profile after single bolus administration with Cmax 0.6, 1.2, 2.4 and 4.8 mg/L. Fungal growth was monitored up to 72h based on galactomannan production. Complete growth inhibition was observed only against A. fumigatus with amphotericin B Cmax ≥2.4 mg/L. At lower Cmaxs 0.6 and 1.2 mg/L, a significant growth delay of 34h and 52h was observed, respectively (pA flavus>A. terreus in the in vitro PK/PD model possibly reflecting the different concentration- and time-dependent inhibitory/killing activities amphotericin B exerting against these species

A comparison of fluoride uptake, enamel surface hardness and surface remineralization using three different fluoride varnishes: in vitro study

OBJECTIVES: To compare the efficacy of fluoride varnishes for their ability to deliver fluoride and re-mineralize human enamel in vitro. METHODS: Three 5% NaF varnishes were used in this study: I. ProFluorid (VOCO), II. Vanish (3M ESPE), III. StarBright (Nanova Biomaterials) and IV. artificial saliva solution as a control. Twenty-four extracted intact adult teeth were randomly divided into 4 groups (n=12 per group). Each group was tested under two protocols (n=6 for each protocol). In Protocol A, artificial lesions were created by immersing the sound teeth in Coca Cola for 20 mins at 37 °C for demineralization (DM). Then a fluoridation step was performed by applying 3 mg of fluoride varnish as a thin layer (RM1). For the control group, artificial saliva was applied. All specimens were submerged in 30 mL of artificial saliva for 24 hr at 37°C. Following the treatment period, extra F varnish was removed from each specimen using chloroform moistened cotton swab and all teeth were then cleaned with deionized water for 10 seconds. The fluoridation cycle was repeated once more (RM2). For subgroups under Protocol B, tooth specimens with sound enamel were treated with two cycles of fluoridation (RM1 and RM2), then exposed to Coca Cola for 20 mins demineralization (DM). Surface micro-hardness of each tooth was measured at three random locations using Knoop hardness and fluoride content of each tooth was analyzed under (SEM/EDS) at baseline, after each fluoride varnish application, and demineralization treatment. The mean Knoop hardness and fluoride content by weight were calculated and the differences within treatment groups were analyzed by JMP Pro 13 using ANOVA. RESULTS: The application of all fluoride varnishes significantly increased the fluoride content of the lesioned enamel (p<0.05). ProFluorid varnish had increased the enamel surface hardness significantly compared to the other two varnishes (StarBright and Vanish), and the difference was statistically significant (p < 0.05). CONCLUSIONS: This study concludes that application of NaF varnish twice can significantly increase the fluoride content in enamel in both remineralization and protection cases, However, the twice application would increase surface hardness of enamel in artificial caries protocol only

Higher levels of osteoprotegerin and immune activation/immunosenescence markers are correlated with concomitant bone and endovascular damage in HIV-suppressed patients

HIV-infected patients appear to have a significantly greater risk of non-AIDS comorbidities such as osteoporosis and atherosclerosis. Subjects with osteoporosis are at a higher risk of developing cardiovascular disease than those with normal bone mass, therefore a possible relation between these two conditions can be hypothesized. In the setting of HIV infection, several factors might contribute to bone disease and endothelial dysfunction. The aim of our study was to evaluate the relationship between bone and cardiovascular disease and to investigate the role of traditional factors, T-cell phenotype and osteoprotegerin in HIV positive subjects on effective antiretroviral therapy. We included 94 HIV positive subjects on antiretroviral therapy with virological suppression and 41 healthy subjects matched for age and gender as a control group. Carotid-Intima Media Thickness (c-IMT) and bone mineral density (BMD) were performed by ultrasound and DEXA, respectively. CD4+/CD8+ T-cell activation, senescence and osteoprotegerin plasma levels were measured by flow-cytometry and ELISA, respectively. Among HIV positive patients, 56.4% had osteopenia/osteoporosis and 45.7% had pathological c-IMT (&gt;0.9mm). Subjects with pathological c-IMT and BMD exhibited higher CD4+ and CD8+ activated, CD8+ senescent and osteoprotegerin than subjects with normal c-IMT and BMD. HIV positive subjects with osteopenia/osteoporosis had higher c-IMT than subjects with normal BMD, and linear regression analysis showed a negative correlation between BMD and c-IMT. Several factors are implicated in the pathogenesis of non-AIDS comorbidities in HIV positive patients. Osteoprotegerin together with inflammation and immunosenescence in HIV positive patients could affect bone and vascular system and could be considered as a possible common link between these two diseases

N-acetylcysteine (NAC) ameliorates Epstein-Barr virus latent membrane protein 1 induced chronic inflammation

Chronic inflammation results when the immune system responds to trauma, injury or infection and the response is not resolved. It can lead to tissue damage and dysfunction and in some cases predispose to cancer. Some viruses (including Epstein-Barr virus (EBV)) can induce inflammation, which may persist even after the infection has been controlled or cleared. The damage caused by inflammation, can itself act to perpetuate the inflammatory response. The latent membrane protein 1 (LMP1) of EBV is a pro-inflammatory factor and in the skin of transgenic mice causes a phenotype of hyperplasia with chronic inflammation of increasing severity, which can progress to pre-malignant and malignant lesions. LMP1 signalling leads to persistent deregulated expression of multiple proteins throughout the mouse life span, including TGFα S100A9 and chitinase-like proteins. Additionally, as the inflammation increases, numerous chemokines and cytokines are produced which promulgate the inflammation. Deposition of IgM, IgG, IgA and IgE and complement activation form part of this process and through genetic deletion of CD40, we show that this contributes to the more tissue-destructive aspects of the phenotype. Treatment of the mice with N-acetylcysteine (NAC), an antioxidant which feeds into the body’s natural redox regulatory system through glutathione synthesis, resulted in a significantly reduced leukocyte infiltrate in the inflamed tissue, amelioration of the pathological features and delay in the inflammatory signature measured by in vivo imaging. Reducing the degree of inflammation achieved through NAC treatment, had the knock on effect of reducing leukocyte recruitment to the inflamed site, thereby slowing the progression of the pathology. These data support the idea that NAC could be considered as a treatment to alleviate chronic inflammatory pathologies, including post-viral disease. Additionally, the model described can be used to effectively monitor and accurately measure therapies for chronic inflammation

Implementation of an effective time-saving two-stage methodology for microstructural characterization of cemented carbides

Linear intercept on scanning electron microscopy micrographs is the most commonly used measurement method to determine carbide grain size and contiguity in WC–Co cemented carbides (hardmetals). However, it involves manual time-consuming measurements and is critically dependent on the quality of the micrographs as well as on the identification and definition of grain boundaries. In this study a two-stage methodology for microstructural characterization of hardmetals is presented. First, a digital semi-automatic image analysis procedure for grain size determination of the carbide phase is presented. It involves an experimental assessment of grain size on processed images corresponding to a series of WC–Co and WC–Ni cemented carbide grades with different microstructural characteristics. Obtained results are then compared to the values obtained by means of the linear intercept technique. A good correlation between the mean grain sizes determined following both measurement techniques was attained. Based on experimental findings, a series of empirical relations were found to correlate grain size distributions obtained following both methods. Second, an empirical relation for estimating carbide contiguity in WC–Co cemented carbides is proposed. This relation considers simultaneously the influence of the binder content and the experimentally determined mean grain size on contiguity. The proposed equation for contiguity estimation is based on extensive data collection from open literature. An excellent agreement was attained between contiguity values estimated from such equation and those obtained using the linear intercept technique. This validates the two-stage procedure as an effective time-saving methodology for microstructural characterization of WC–Co cemented carbides.Peer ReviewedPostprint (author's final draft