Use of 16S rRNA Gene Based Clone Libraries to Assess Microbial Communities Potentially Involved in Anaerobic Methane Oxidation in a Mediterranean Cold Seep

This study provides data on the diversities of bacterial and archaeal communities in an active methane seep at the Kazan mud volcano in the deep Eastern Mediterranean sea. Layers of varying depths in the Kazan sediments were investigated in terms of (1) chemical parameters and (2) DNA-based microbial population structures. The latter was accomplished by analyzing the sequences of directly amplified 16S rRNA genes, resulting in the phylogenetic analysis of the prokaryotic communities. Sequences of organisms potentially associated with processes such as anaerobic methane oxidation and sulfate reduction were thus identified. Overall, the sediment layers revealed the presence of sequences of quite diverse bacterial and archaeal communities, which varied considerably with depth. Dominant types revealed in these communities are known as key organisms involved in the following processes: (1) anaerobic methane oxidation and sulfate reduction, (2) sulfide oxidation, and (3) a range of (aerobic) heterotrophic processes. In the communities in the lowest sediment layer sampled (22–34 cm), sulfate-reducing bacteria and archaea of the ANME-2 cluster (likely involved in anaerobic methane oxidation) were prevalent, whereas heterotrophic organisms abounded in the top sediment layer (0–6 cm). Communities in the middle layer (6–22 cm) contained organisms that could be linked to either of the aforementioned processes. We discuss how these phylogeny (sequence)-based findings can support the ongoing molecular work aimed at unraveling both the functioning and the functional diversities of the communities under study

Cold Seep Systems

‘Cold’ seeps (or cold vents) are seafloor manifestations of fluid migration through sediments from the subsurface to the seabed and into the water column, and may reach the atmosphere. They are an important but not fully understood process in our oceans that has important repercussions on human society and on the climate. Modern sonar systems can obtain seafloor images of cold seep features from tens to thousands of meters wide with metric resolution, providing key information on the formation and evolution of the various seabed expressions of cold seeps. In this chapter we attempt to address cold seep systems with an emphasis on their origin, evolution, form, and occurrence, approaching them primarily from their morphologies and the acoustic character of the seafloor and near bottom erupted sediments. We address morphological characteristics of mud volcanoes, pockmarks, carbonate-related structures including MDAC, AOM and giant carbonate mounds and ridges, offering various examples mainly from recent discoveries in Mediterranean region which are among the most spectacular and most frequently cited examples. Detailed focus on topics such as acoustic backscatter, brine pools, etc. have been described in separate gray boxes of text with the aim to highlight their particular significance. Finally, gaps in knowledge and key research questions on cold seep studies have been outlined with the aim of orienting young researchers and students towards those topics that deserve the highest attention as they are still unresolved.Istituto Nazionale di Oceanografia e di Geofisica Sperimentale, ItaliaFREMER Laboratoire Aléas géologiques et Dynamique Sédimentaire Centre Bretagne, FranciaÁrea de Geología Marina, Instituto Geológico y Minero de España, EspañaLyngby Marine Geophysical Research, Países BajosPeer reviewe

Acoustic monitoring of gas emissions from the seafloor. Part II: a case study from the Sea of Marmara

A rotating, acoustic gas bubble detector, BOB (Bubble OBservatory) module was deployed during two surveys, conducted in 2009 and 2011 respectively, to study the temporal variations of gas emissions from the Marmara seafloor, along the North Anatolian Fault zone. The echosounder mounted on the instrument insonifies an angular sector of 7° during a given duration (of about 1 h). Then it rotates to the next, near-by angular sector and so forth. When the full angular domain is insonified, the “pan and tilt system” rotates back to its initial position, in order to start a new cycle (of about 1 day). The acoustic data reveal that gas emission is not a steady process, with observed temporal variations ranging between a few minutes and 24 h (from one cycle to the other). Echo-integration and inversion performed on the acoustic data as described in the companion paper of Leblond et al. (Mar Geophys Res, 2014), also indicate important variations in, respectively, the target strength and the volumetric flow rates of individual sources. However, the observed temporal variations may not be related to the properties of the gas source only, but reflect possible variations in sea-bottom currents, which could deviate the bubble train towards the neighboring sector. During the 2011 survey, a 4-component ocean bottom seismometer (OBS) was co-located at the seafloor, 59 m away from the BOB module. The acoustic data from our rotating, monitoring system support, but do not provide undisputable evidence to confirm, the hypothesis formulated by Tary et al. (2012), that the short-duration, non-seismic micro-events recorded by the OBS are likely produced by gas-related processes within the near seabed sediments. Hence, the use of a multibeam echosounder, or of several split beam echosounders should be preferred to rotating systems, for future experiments