61 research outputs found
Manual de produção de uvas viníferas de alta qualidade.
bitstream/item/129038/1/MANUAL-de-PRODUCAO-de-UVAS-VINIFERAS-de-ALTA-QUALIDADE-2015-Revisado-em-31-08-15.pd
Protective effect of stromal Dickkopf-3 in prostate cancer: opposing roles for TGFBI and ECM-1
Aberrant transforming growth factor–β (TGF-β) signaling is a hallmark of the stromal microenvironment in cancer. Dickkopf-3 (Dkk-3), shown to inhibit TGF-β signaling, is downregulated in prostate cancer and upregulated in the stroma in benign prostatic hyperplasia, but the function of stromal Dkk-3 is unclear. Here we show that DKK3 silencing in WPMY-1 prostate stromal cells increases TGF-β signaling activity and that stromal cellconditioned media inhibit prostate cancer cell invasion in a Dkk-3-dependent manner. DKK3 silencing increased the level of the cell-adhesion regulator TGF-β–induced protein (TGFBI) in stromal and epithelial cell-conditioned media, and recombinant TGFBI increased prostate cancer cell invasion. Reduced expression of Dkk-3 in patient tumors was associated with increased expression of TGFBI. DKK3 silencing reduced the level of extracellular matrix protein-1 (ECM-1) in prostate stromal cell-conditioned media but increased it in epithelial cell-conditioned media, and recombinant ECM-1 inhibited TGFBI-induced prostate cancer cell invasion. Increased ECM1 and DKK3 mRNA expression in prostate tumors was associated with increased relapse-free survival. These observations are consistent with a model in which the loss of Dkk-3 in prostate cancer leads to increased secretion of TGFBI and ECM-1, which have tumor-promoting and tumor-protective roles, respectively. Determining how the balance between the opposing roles of extracellular factors influences prostate carcinogenesis will be key to developing therapies that target the tumor microenvironment
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Differentiating basal cell carcinoma from intradermal nevi on the eyelid: The utility of dermoscopy
Thiosulphate oxidation by Thiobacillus thioparus and Halothiobacillus neapolitanus strains isolated from the petrochemical industry
Sulphur Oxidizing Bacteria (SOB) is a group of microorganisms widely
used for the biofiltration of Total Reduced Sulphur compounds (TRS).
TRS are bad smelling compounds with neurotoxic activity which are
produced by different industries (cellulose, petrochemical).
Thiobacillus thioparus has the capability to oxidize organic TRS, and
strains of this bacterium are commonly used for TRS biofiltration
technology. In this study, two thiosulphate oxidizing strains were
isolated from a petrochemical plant (ENAP BioBio, Chile). They were
subjected to molecular analysis by real time PCR using specific primers
for T. thioparus. rDNA16S were sequenced using universal primers and
their corresponding thiosulphate activities were compared with the
reference strain T. thioparus ATCC 10801 in batch standard conditions.
Real time PCR and 16S rDNA sequencing showed that one of the isolated
strains belonged to the Thiobacillus branch. This strain degrades
thiosulphate with a similar activity profile to that shown by the ATCC
10801 strain, but with less growth, making it useful in biofiltration
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