Molecular characterization of bacteria isolated from the Kingdom of Saudi Arabia and their uses against pathogenic fungi causing dermatological diseases

Random amplified polymorphic DNA (RAPD) assay and antimicrobial activities were used for the detection of genetic variations of antimicrobial isolates from the Kingdom Saudi of Arabia. Soil samples were collected  from El-Madina El-Monawara, El–Taif, El-Jouf and El-Dammam for bacterial isolation. Bacterial isolates were  evaluated for their antimicrobial activities against pathogenic fungi which cause dermatological diseases;  Acremonium sp., Microsporium gypseum, Microsporium gallinae, Exserohilum sp., Trichphyton sp. and  Aspergillus niger. Results indicate that all the bacterial isolates showed different levels of antagonistic  activities against the pathogenic fungi. Esherichia coli M2, Bacillus megaterium M3 and Bacillus subtilis M10  showed strong effect against all the tested pathogenic fungi. B. subtilis J1 isolate showed strong effect against  the two pathogenic fungi M. gallinae and Exserohilum sp. Nine strains were tested for their antibiotic response. The results show that different antibiotic response was found. RAPD fingerprinting technique was applied to  study the genetic diversity among the tested strains. Results indicate that there was a significant genomic  diversity among the B. subtilis strains and this diversity was highly correlated with habitats and the  antagonistic effect of the studied strains.Key words: Random amplified polymorphic DNA-polymerase chain reaction (RAPD-PCR), bacterial isolates, pathogenic fungi, Kingdom of Saudi Arabia

Central Nervous System Depressant, Analgesic and Antidiarrheal Effects of the Seed Extracts of Dimocarpus longan Lour in Rats

Purpose: To assess the central nervous system (CNS) depressant, analgesic and antidiarrheal activities of the dried seed crude extracts of Dimocarpus longan Lour in rodents.Methods: Selected pharmacological effects of the ethanol (ENLS), petroleum ether (PELS), chloroform (CHLS) and ethyl acetate (EALS) extracts of D. longan fruit seeds were investigated. CNS depressant activity was evaluated by open field and hole cross tests; analgesic activity by acetic acid-induced writhing test and formalin-induced licking test; and anti-diarrheal activity was assessed in castor oil and magnesium-induced diarrhea rat model. The extracts were given orally in a rat model at doses of 200 and 300 mg/kg body weight. Normal saline served as control in all experiment. In CNS depressant test, diazepam (1 mg/kg) was used as reference drug while indomethacin (10 mg/kg) and loperamide(2 mg/kg) were used as standard drugs in analgesic and antidiarrheal tests, respectively.Results: In hole cross method, EALS showed the most effective depressant effect, viz, 1.17±0.17 for 200 mg/kg dose and 0.83±0.31 number of movements for 300 mg/kg dose after 120 min (p < 0.01), whereas in the open field test, all the extracts exhibited significant (p < 0.01) depressant effect in relation to positive control, diazepam. In acetic acid-induced pain test, PELS gave the lowest number of writhing (2.83±0.307) and the highest inhibition (88.45 %, 300 mg/kg dose) which was statistically significant. All the extracts also significantly (p < 0.01) suppressed licking activity in both phases of the formalin-induced licking test, in contrast to indomethacin. In the antidiarrheal tests, diarrheal suppression was highest at 300 mg/kg dose for all the extracts, compared with loperamide in both castor oil and magnesium sulphate induced diarrhea model.Conclusion: The extracts of Dimocarpus longan tested demonstrated significant CNS depressant, analgesic and antidiarrheal activities in a rodent model.Keywords: Dimocarpus longan Lour, CNS depressant, Analgesic, Anti-diarrheal

Nutritional status of adolescent girls in a selected secondary school of north-eastern part of Nigeria

Background: Adolescence is the most critical period of human life when transitioning occurs from childhood to adulthood. Malnutrition is one of the major global health problems faced by many developing countries across the globe. Objectives: This study aimed to investigate the nutritional status of adolescent girls in a selected secondary school in Nigeria. Methods: A cross-sectional study was conducted on 250 adolescent girls who were available during the study period. A nutrition expert, together with an epidemiologist, gathered anthropometric information and measured the height, weight, and body mass index (BMI) of the participants. The BMI was calculated, and the dietary habits of the participants were reported based on their usual food habits. It is part of our limitations and has been addressed under the limitations. Data were collected using a self-administered and semi-structured questionnaire. Results: The mean age of the adolescent girls was 15.9 ± 0.9 years, and more than half (53.2%) were students of senior secondary class 1 (SS-1). More than half (54.4%) of the adolescent girls had average body weight, 36.0% were underweight, and only 9.6% were overweight. The following socio-demographic factors were significantly associated with the BMI of adolescent girls: Age, class of the students, monthly family income, mothers’ educational status, and area of residence. Close to four-fifths (78.4%) of the participants consumed rice more than twice in a week; more than eight-tenths (88.8%) of the participants consumed milk/milk products at least ≤ 2 in a week. About 84.4% of the participants took red meat at least ≤ 2 in a week; more than half (55.2%) of the participants consumed vegetables and fruits more than twice a week. Most (84.8%) of the participants took lunch regularly, and 91.6% of the adolescent girls took breakfast regularly. Conclusions: The study revealed that nearly one-third of the adolescent girls were underweight, indicating a severe public health concern. Early nutritional screening and interventions are recommended to improve the nutritional status of the adolescent girl school in Nigeria

Terahertz VRT spectroscopy of the water hexamer-d<inf>12</inf> prism: Dramatic enhancement of bifurcation tunneling upon librational excitation

Using diode laser vibration-rotation-tunneling spectroscopy near 15 Thz (500 cm−1), we have measured and assigned 142 transitions to three a-type librational subbands of the water hexamer-d12 prism. These subbands reveal dramatically enhanced (ca. 1000×) tunneling splittings relative to the ground state. This enhancement is in agreement with that observed for the water dimer, trimer, and pentamer in this same frequency region. The water prism tunneling motion has been predicted to potentially describe the motions of water in interfacial and confined environments; hence, the results presented here indicate that excitation of librational vibrations has a significant impact on the hydrogen bond dynamics in these macroscopic environments.</jats:p

Factors associated with treatment gap in children and adolescents with epilepsy in a rural Nigerian community.

Background: The campaign against epilepsy is hampered by the difference between those with the active disorder and the number of them receiving appropriate treatment (treatment gap) in sub-Saharan Africa. Identifying the determinants of this gap is crucial to providing and achieving optimal care.Objective: To identify the determinants of epilepsy treatment gap (ETG) in children and adolescents (Subjects) with epilepsy in a rural community.Methods: Subjects were identified through a community house to house survey. Information obtained from Subjects and their care givers included: sociodemographic characteristics, type and frequency of epileptic seizures,current and past treatment options utilized, reasons for treatment options used, and treatment options utilized for other health complaints.Results: Twenty three Subjects (6.4/1000 of the child and adolescent population) were identified as having epilepsy. Their age range was 4-19 years (mean 14.3±4.7 years). Most were males (82.6%) and adolescents (78.3%). Seizures were mostly generalized (95.7%) and occurred most frequently daily. Current treatment modalities were use of traditional medication (100%) and prayers (34.8%).None was currently on orthodox medical therapy (ETG, 100%) but 5(21.7%) had utilized orthodox medical therapy in the past. The main determinants of the ETG were strong cultural belief, weakness in the health system to epilepsy treatment and low socioeconomic status. Fever was the commonest other health complaint and use of orthodox medical therapy was significantly (p˂ 0.05) the main (16, 69.6%) treatment option utilized.Conclusion: Cultural belief, weak health system and low socioeconomic status were determinants of an absolute ETG. It highlights the need to strengthen initiatives that enhance accessibility to standard epilepsy treatment.Key words: Epilepsy treatmentgap, determinants, children, adolescents, rural communit

Very special relativity as relativity of dark matter: the Elko connection

In the very special relativity (VSR) proposal by Cohen and Glashow, it was pointed out that invariance under HOM(2) is both necessary and sufficient to explain the null result of the Michelson-Morely experiment. It is the quantum field theoretic demand of locality, or the requirement of P, T, CP, or CT invariance, that makes invariance under the Lorentz group a necessity. Originally it was conjectured that VSR operates at the Planck scale; we propose that the natural arena for VSR is at energies similar to the standard model, but in the dark sector. To this end we provide an ab initio spinor representation invariant under the SIM(2) avatar of VSR and construct a mass dimension one fermionic quantum field of spin one half. This field turns out to be a very close sibling of Elko and it exhibits the same striking property of intrinsic darkness with respect to the standard model fields. In the new construct, the tension between Elko and Lorentz symmetries is fully resolved. We thus entertain the possibility that the symmetries underlying the standard model matter and gauge fields are those of Lorentz, while the event space underlying the dark matter and the dark gauge fields supports the algebraic structure underlying VSR.Comment: 19 pages. Section 5 is new. Published version (modulo a footnote, and a corrected typo

Global Initiative for Asthma (GINA) strategy 2021 - executive summary and rationale for key changes.

The Global Initiative for Asthma (GINA) Strategy Report provides clinicians with an annually updated evidence-based strategy for asthma management and prevention, which can be adapted for local circumstances (e.g., medication availability). This article summarizes key recommendations from GINA 2021, and the evidence underpinning recent changes. GINA recommends that asthma in adults and adolescents should not be treated solely with short-acting beta2-agonist (SABA), because of the risks of SABA-only treatment and SABA overuse, and evidence for benefit of inhaled corticosteroids (ICS). Large trials show that as-needed combination ICS-formoterol reduces severe exacerbations by ≥60% in mild asthma compared with SABA alone, with similar exacerbation, symptom, lung function and inflammatory outcomes as daily ICS plus as-needed SABA. Key changes in GINA 2021 include division of the treatment figure for adults/adolescents into two tracks. Track 1 (preferred) has low-dose ICS-formoterol as the reliever at all steps: as-needed only in Steps 1-2 (mild asthma), and with daily maintenance ICS formoterol (maintenance-and-reliever therapy, MART) in Steps 3-5. Track 2 (alternative) has as-needed SABA across all steps, plus regular ICS (Step 2) or ICS-long-acting beta2-agonist (LABA) (Steps 3-5). For adults with moderate-to-severe asthma, GINA makes additional recommendations in Step 5 for add-on long-acting muscarinic antagonists and azithromycin, with add-on biologic therapies for severe asthma. For children 6-11 years, new treatment options are added at Steps 3-4. Across all age-groups and levels of severity, regular personalized assessment, treatment of modifiable risk factors, self-management education, skills training, appropriate medication adjustment and review remain essential to optimize asthma outcomes

Facilitating the Recruitment of Minority Ethnic People into Research: Qualitative Case Study of South Asians and Asthma

Aziz Sheikh and colleagues report on a qualitative study in the US and the UK to investigate ways to bolster recruitment of South Asians into asthma studies, including making inclusion of diverse populations mandatory

Beyond Logarithmic Corrections to Cardy Formula

As shown by Cardy modular invariance of the partition function of a given unitary non-singular 2d CFT with left and right central charges c_L and c_R, implies that the density of states in a microcanonical ensemble, at excitations Delta and Delta-bar and in the saddle point approximation, is \rho_0(\Delta,\bar\Delta;c_L, c_R)=c_L c_R \exp(2\pi\sqrt{{c_L\Delta}/{6}})\exp(2\pi\sqrt{{c_R\bar\Delta}/{6}}). In this paper, we extend Cardy's analysis and show that in the saddle point approximation and up to contributions which are exponentially suppressed compared to the leading Cardy's result, the density of states takes the form \rho(\Delta,\bar\Delta; c_L,c_R)= f(c_L\Delta) f(c_R\bar\Delta)\rho_0(\Delta,\bar\Delta; c_L, c_R), for a function f(x) which we specify. In particular, we show that (i) \rho (\Delta,\bar\Delta; c_L, c_R) is the product of contributions of left and right movers and hence, to this approximation, the partition function of any modular invariant, non-singular unitary 2d CFT is holomorphically factorizable and (ii) \rho(\Delta,\bar\Delta; c_L, c_R)/(c_Lc_R) is only a function of $c_R\bar\Delta$ and $c_L\Delta$. In addition, treating \rho(\Delta,\bar\Delta; c_L, c_R) as the density of states of a microcanonical ensemble, we compute the entropy of the system in the canonical counterpart and show that the function f(x) is such that the canonical entropy, up to exponentially suppressed contributions, is simply given by the Cardy's result \ln\rho_0(\Delta,\bar\Delta; c_L, c_R).Comment: 30 pages, no figures; v2: minor improvements, one reference added, v3: minor corrections to match the published versio

Detection and Diagnosis of Stator and Rotor Electrical Faults for Three-Phase Induction Motor via Wavelet Energy Approach

This paper presents a fault detection method in three-phase induction motors using Wavelet Packet Transform (WPT). The proposed algorithm takes a frame of samples from the three-phase supply current of an induction motor. The three phase current samples are then combined to generate a single current signal by computing the Root Mean Square (RMS) value of the three phase current samples at each time stamp. The resulting current samples are then divided into windows of 64 samples. Each resulting window of samples is then processed separately. The proposed algorithm uses two methods to create window samples, which are called non-overlapping window samples and moving/overlapping window samples. Non-overlapping window samples are created by simply dividing the current samples into windows of 64 samples, while the moving window samples are generated by taking the first 64 current samples, and then the consequent moving window samples are generated by moving the window across the current samples by one sample each time. The new window of samples consists of the last 63 samples of the previous window and one new sample. The overlapping method reduces the fault detection time to a single sample accuracy. However, it is computationally more expensive than the non-overlapping method and requires more computer memory. The resulting window samples are separately processed as follows: The proposed algorithm performs two level WPT on each resulting window samples, dividing its coefficients into its four wavelet subbands. Information in wavelet high frequency subbands is then used for fault detection and activating the trip signal to disconnect the motor from the power supply. The proposed algorithm was first implemented in the MATLAB platform, and the Entropy power Energy (EE) of the high frequency WPT subbands’ coefficients was used to determine the condition of the motor. If the induction motor is faulty, the algorithm proceeds to identify the type of the fault. An empirical setup of the proposed system was then implemented, and the proposed algorithm condition was tested under real, where different faults were practically induced to the induction motor. Experimental results confirmed the effectiveness of the proposed technique. To generalize the proposed method, the experiment was repeated on different types of induction motors with different working ages and with different power ratings. Experimental results show that the capability of the proposed method is independent of the types of motors used and their ages