Study on rearing movine carps in the ponds of brackish water and freshwater

In this study, mono culture of Cyprinus carpio in fresh water and brackish water with 3500 specimens per hectare with 3 replication each carriedout from midel April to end the October 2010 and mide april to end the October 2011 recptively. Juline fish with 40 gr weight takeup from the sade voshmgir and after prepara tion of rearing ponds were introduced to pond according to appropriate and caluclate stocking density. Juline were fed by concentracted food(GFC). Feeding calculation based on percentage of body weight varied from 5 to 10 % of body weight. In fresh water Average weight and lenghth were in the stage time stocking 40 gr 14.68 cm and at the end of cultuivation period were 704.26 gr and 37.04 cm respectively. Inorder to comparecultivation of Cyprinus carpio in freshwater with breackish water another stocking 350 specimens per hactar with 3 replications also tookplace in this study. Junlin fish with average weight of 40gr and average length of 14.12cm were introduced to the ponds. At the end of rearing period average final weight was 700.26 with average length of 38.86cm.mean daily growth in fresh water treatment was 3.39. in brackish water replication the average daily . average condition factor in fresh water was 2.41. The average value CF in breackishwater was 1.39. in this research was survival rate in the fresh water and breackish water were more 77%

The study of annual cycle of gonads developments and sex steroids of gray mullet, Mugil cephalus in Golestan Province

Grey mullet, Mugil cephalus is one of the most famous and valuable fish on the fisheries and aquaculture industry. This species is the most suitable to introducing the southern, northern and central regions of our country due to high resistant to large variation of water salinity and temperature. On the monthly sampling period biometric data including total length and total weight recorded and length and weight classes for every sex were determined. To understanding of physiological functions and sexual development of reproductive organs, this investigation carried out with Grey mullet imported from Hong Kong by 1993. Findings of this research can be used for management of artificial breeding of this species. Daily variation of pond water temperature was recorded. Sex steroid hormones and other plasma metabolites such as Ca^2+, cholesterol, triglycerides and total proteins were measured by blood sampling. Gonad samples were prepared in order to histological studies. Oocyte growth was studied by measurement of egg diameters. Measured values of cholesterol, triglycerides of blood plasma revealed that these metabolites have seasonal variation and severely deposited on the summer and mobilized on the spawning seasons. Findings of this research suggested that egg diameters, values of sex steroid hormones (estradiol and progesterone) and Calcium concentrations of fish blood plasma shortening increased a long with day length shortening

Fisebility study of collection and rearing of proleus stage of lobster (Panolirus homarus) in the cement tanks

The present study was carried out from late October2010 to end of June 2011. Executive operations were done in two stages; proleus catching from the sea and stocking/rearing them in a control conditions. Totally, it took 8 months from start to finish. The research objectives were; - Proleus catching techniques achievement. -Proleus keeping, stoking and rearing achievement. 32 pieces of proleus and post-proleus were collected by special installed collectors at the beneath of the sea. They were stocked after adaptation and initially biometry. In order to stocking, two 4th-tone tanks (one for proleus stage and another one for post-proleus stage) were applied. 0.77 gram and 11.8 gram was recorded as average weighted for proleus and post- proleus, respectively. Four stations were chosen for collecting of require samples. They were starched from west side of Ramin’s Jetty toward east. 84% of the samples were caught from the station two, using sandwich collectors. Collector’s efficiencies were compared to each others, in this study. A significant difference was observed between the sandwich collectors efficiency with the other collectors (p0.05). Comparison of the proleus abundance between stations showed a significant difference between station 2 and other stations, but there wasn’t any sig. difference between the stations number 2, 3 and 4, together (p>0.05). The samples were fed by fish waste, bivalves and squid. The amount of larval requirement food was calculated based on 15% of their body weight. During breading period, 50% of the rearing tanks water was exchanged, daily. Total weight, total length and carapace length was measured, monthly. 0.6 gram, 5.5 mm and 1 mm was recorded for mean total weight, mean total length and mean carapace length for proleus and 18.3 gram, 25.4 mm and 8 mm was recorded for mean total weight, mean total length and mean carapace length for post-proleus. According to the results, can be realized that growth rate in post-proleus was much faster than the growth rate of proleus. Finally, comparison of the total weight, total length and carapace length between proleus and post-proleus were showed a sig. differences between the two reared groups (p>0.05)

GaAs LASER RHEUMATISMAL BIOSTIMULOTHERAPY

The paper deals with the construction of a pulsed laser diode generator for medical purposes, using avalanche transistors. The promising results obtained with such a generator in rheumatismal biostimulotherapy are also discussed

EXPRESSION OF ALKALINE PHOSPHATASE DURING OSTEOGENIC DIFFERENTIATION OF RAT BONE MARROW STROMAL CELLS

Introduction: Bone marrow contains a population of stem cells capable of differentiating to osteoblast and forming the bone nodule by dexamethasone. Material and Methods: The stromal cells of bone marrow obtained from 4 to 6 weeks old Spruge-Dawely male rats were grown in primary culture for 7 days and subcultured for 18 days. The cells were cultured in either DMEM medium containing 15% fetal calf serum and antibiotics as the controls or the above medium supplemented with osteogenic supplements (OS): include 10 mM Na-beta glycerophosphate (Na-betaGp), 10 nM dexamethasone (Dex) and 50 g/ml ascordic acid (AsA) as the examined cultures. After 6, 12 and 18 days of grow up in subculture, the cultures were examined for mineralization and alkaline phosphatase (Apase) expression. Results: Mesenchymal stem cells (MSCs) in examined cultures underwent a dramatic change in cellular morphology and a significat increase in Apase activity by day 12. The deposition of a calcified matrix on the surface of the culture flasks became evident between days 12 and 18. Conclusion: The addition of osteogenic supplements (OS) to MSCs cultures induced Apase expression that contributes to cellular differentiation and mineralization of extracellular matrix

Introduction of a new method for preparation of intact bones from fixed cadavers and its qualitative evaluation

History and Objectives: It is of high importance to use intact and normal bones in training of anatomy courses. Considering the relative deficiency and the low quality of the present materials, this study was designed to find out a new method of preparation of normal bones from fixed cadavers in 1999. Materials and Methods: In this study the bones from cadavers fixed with formalin, phenol, alcohol and glycerin were used in anatomy department of Tehran university of medical sciences. For this purpose, the soft tissues were removed from the bones by surgical scissors and scalpel and for complete removal of these tissues, the bones were immersed in sodium hypochloride solution for 24h and then after rinsing and drying in oven at 50°C, they were placed in cerosene-ethanol solution for fat removal. In the next stage, they were dried in the open environment and were bleached by 5 hydrogen peroxide solution. Finally, a layer of oil was painted on the bones. The prepared bones by this method were compared with the existing bones regarding quality and anatomic structure. Results: The prepared bones by this method showed no fracture and decay and their normal integrity was maintained and they were more healthy to use and it is more easier to clean them and more cheaper to prepare them in large scale. Conclusion: The above-mentioned method is recommended to prepare bones with higher quality and it is better to use them in teaching of anatomy courses. Furthermore, it is recommended to perform more studies using other materials for preparation of intact bones

COUPLED MODES NUMBER EVALUATION FOR FM MODE-LOCKED LASERS.

This paper analyses the frequency modulated gas laser operation and mode-locking, achieved by phase perturbation of the cavity medium, using Lamb's semiquantum model of the laser. The most important parameters of the perturbed system which are taken into account in the established expression of the modes number are: the modulation frequency detuning, the perturbation strength, the gain and saturation coefficients

Sequential en-face optical coherence tomography imaging and monitoring of Drosophila Melanogaster larval heart

This article demonstrates two modalities to acquire information on cardiac function in larval Drosophila Melanogaster: in-vivo imaging and heartbeat monitoring. To achieve these goals a dedicated imaging instrument able to provide simultaneous en-face Optical Coherence Tomography (OCT) and Laser Scanning Confocal Microscopy (LSCM) images has been developed. With this dual imaging system, the heart can easily be located and visualised within the specimen and the change of the heart shape in a cardiac cycle monitored. The system can easily be switched to a stethoscopic regime, simply by interrupting the scanning of the light beam across the sample, after selecting the point of interest in the imaging regime. Here we have used targeted gene expression to knockdown the myospheroid (mys) gene in the larval heart using a specific RNAi construct. By knocking down a β integrin subunit encoded by mys we have recorded an enlarged heart chamber in both diastolic and systolic states. Also, the fraction of reduction of the chamber diameter was smaller in the knockdown heart. These phenotypic differences indicate that impaired cardiac contractility occurs in the heart where the integrin gene express level is reduced. As far as we are aware, this is for the first time when it is shown in Drosophila that integrins have a direct relationship to a dilated heart defect, and conseqThis article demonstrates two modalities to acquire information on cardiac function in larval Drosophila Melanogaster: in-vivo imaging and heartbeat monitoring. To achieve these goals a dedicated imaging instrument able to provide simultaneous en-face Optical Coherence Tomography (OCT) and Laser Scanning Confocal Microscopy (LSCM) images has been developed. With this dual imaging system, the heart can easily be located and visualised within the specimen and the change of the heart shape in a cardiac cycle monitored. The system can easily be switched to a stethoscopic regime, simply by interrupting the scanning of the light beam across the sample, after selecting the point of interest in the imaging regime. Here we have used targeted gene expression to knockdown the myospheroid (mys) gene in the larval heart using a specific RNAi construct. By knocking down a β integrin subunit encoded by mys we have recorded an enlarged heart chamber in both diastolic and systolic states. Also, the fraction of reduction of the chamber diameter was smaller in the knockdown heart. These phenotypic differences indicate that impaired cardiac contractility occurs in the heart where the integrin gene express level is reduced. As far as we are aware, this is for the first time when it is shown in Drosophila that integrins have a direct relationship to a dilated heart defect, and consequently we demonstrate the utility of Drosophila as model for the study of vertebrate heart disease. By monitoring the heartbeat we also demonstrated a reduction of the heart rate in Tropomyosin mutant compared to the wild type larva.uently we demonstrate the utility of Drosophila as model for the study of vertebrate heart disease. By monitoring the heartbeat we also demonstrated a reduction of the heart rate in Tropomyosin mutant compared to the wild type larva

Dispositif bistable à laser He-Ne à cristal liquide dans la cavité couplée

A hybrid bistable optical device is realised by using a liquid crystal light modulator (based on dynamic scattering), placed in a passive cavity which is coupled to a He-Ne laser cavity. The liquid crystal transmission is driven electronically by means of a feedback loop in the device. © 1985

Correlation of quantitative light-induced fluorescence and optical coherence tomography applied for detection and quantification of early dental caries

Fluorescence loss in enamel following demineralization has been correlated with the amount of mineral lost during the demineralization. The correlation between fluorescence loss measured by quantitative light-induced fluorescence (QLF) and the reflectivity loss measured by a versatile en face optical coherence tomography (OCT) system was investigated in a demineralization process to produce artificial dental caries. We used an OCT system that can collect A-scans (reflectivity versus depth), B-scans (longitudinal images), and C-scans (en face images). The power to the sample was 250 μW, the wave-length λ = 850 nm, and the depth resolution in air 16 μm. A-scans, which show the profile of the reflectivity versus the depth of penetration into the tooth tissue, were used for quantitative analysis of the reflectivity loss. The results have shown that both the fluorescence radiance and reflectivity of the enamel decrease with increasing demineralization time. A linear correlation was observed between the percentage of fluorescence loss measured by QLF and the percentage of reflectivity loss measured by OCT. It was concluded that the decrease in reflectivity of the enamel during demineralization, measured by OCT, could be related to the amount of mineral lost during the demineralization process © 2003 Society of Photo-Optical Instrumentation Engineers