Classical BV theories on manifolds with boundary

In this paper we extend the classical BV framework to gauge theories on spacetime manifolds with boundary. In particular, we connect the BV construction in the bulk with the BFV construction on the boundary and we develop its extension to strata of higher codimension in the case of manifolds with corners. We present several examples including electrodynamics, Yang-Mills theory and topological field theories coming from the AKSZ construction, in particular, the Chern-Simons theory, the $BF$ theory, and the Poisson sigma model. This paper is the first step towards developing the perturbative quantization of such theories on manifolds with boundary in a way consistent with gluing.Comment: The second version has many typos corrected, references added. Some typos are probably still there, in particular, signs in examples. In the third version more typoes are corrected and the exposition is slightly change

Fast Algorithm for Partial Covers in Words

A factor $u$ of a word $w$ is a cover of $w$ if every position in $w$ lies within some occurrence of $u$ in $w$. A word $w$ covered by $u$ thus generalizes the idea of a repetition, that is, a word composed of exact concatenations of $u$. In this article we introduce a new notion of $\alpha$-partial cover, which can be viewed as a relaxed variant of cover, that is, a factor covering at least $\alpha$ positions in $w$. We develop a data structure of $O(n)$ size (where $n=|w|$) that can be constructed in $O(n\log n)$ time which we apply to compute all shortest $\alpha$-partial covers for a given $\alpha$. We also employ it for an $O(n\log n)$-time algorithm computing a shortest $\alpha$-partial cover for each $\alpha=1,2,\ldots,n$

Constitutive Activation of STAT5A Promotes Human Hematopoietic Stem Cell Self-Renewal and Erythroid Differentiation

Activation of the transcription factor signal transducer and activator of transcription (STAT)5 is involved in various aspects of hematopoiesis, affecting cell proliferation, differentiation, and cell survival. Constitutive activation of STAT5 has also been associated with leukemic transformation. We overexpressed the constitutively active mutant STAT5A(1*6) in human cord blood CD34+ cells and evaluated the effects on the hematopoietic potential of stem cells in a variety of in vitro and in vivo systems. The observed phenotypic changes were correlated with differential gene expression patterns induced by STAT5A(1*6). Our data indicate that a persistent activation of STAT5A in human hematopoietic stem and progenitor cells results in their enhanced self-renewal and diverts differentiation to the erythroid lineage

Entanglement between motional states of a single trapped ion and light

We propose a generation method of Bell-type states involving light and the vibrational motion of a single trapped ion. The trap itself is supposed to be placed inside a high-$Q$ cavity sustaining a single mode, quantized electromagnetic field. Entangled light-motional states may be readily generated if a conditional measurement of the ion's internal electronic state is made after an appropriate interaction time and a suitable preparation of the initial state. We show that all four Bell states may be generated using different motional sidebands (either blue or red), as well as adequate ionic relative phases.Comment: 4 pages, LaTe

Role of Dimerization of the Membrane-associated Growth Factor Kit Ligand in Juxtacrine Signaling: The Sl17H Mutation Affects Dimerization and Stability—Phenotypes in Hematopoiesis

The Kit ligand (KL)/Kit receptor pair functions in hematopoiesis, gametogenesis, and melanogenesis. KL is encoded at the murine steel (Sl) locus and encodes a membrane growth factor which may be proteolytically processed to produce soluble KL. The membrane-associated form of KL is critical in mediating Kit function in vivo. Evidence for a role of cytoplasmic domain sequences of KL comes from the Sl17H mutation, a splice site mutation that replaces the cytoplasmic domain with extraneous amino acids. Using deletion mutants and the Sl17H allele, we have investigated the role of the cytoplasmic domain sequences of KL in biosynthetic processing and cell surface presentation. The normal KL protein products are processed for cell surface expression, where they form dimers. Both Sl17H and the cytoplasmic deletion mutants of KL were processed to the cell surface; however, the rate of transport and protein stability were affected by the mutations. Deletion of cytoplasmic domain sequences of KL did not affect dimerization of KL. In contrast, dimerization of the Sl17H protein was reduced substantially. In addition, we have characterized the hematopoietic cell compartment in Sl17H mutant mice. The Sl17H mutation has only minor effects on hematopoiesis. Tissue and peritoneal mast cell numbers were reduced in mutant mice as well as in myeloid progenitors. Interestingly, long-term bone marrow cultures from Sl17H mice did not sustain the long-term production of hematopoietic cells. In addition, homing of normal hematopoietic progenitors to the spleen of irradiated Sl17H/Sl17H recipient mice was diminished in transplantation experiments, providing evidence for a role of Kit in homing or lodging. These results demonstrate that the membrane forms of KL exist as homodimers on the cell surface and that dimerization may play an important role in KL/Kit-mediated juxtacrine signaling

Strong lens search in the ESO public Survey KiDS

We have started a systematic search of strong lens candidates in the ESO public survey KiDS based on the visual inspection of massive galaxies in the redshift range $0.1<z<0.5$. As a pilot program we have inspected 100 sq. deg., which overlap with SDSS and where there are known lenses to use as a control sample. Taking advantage of the superb image quality of VST/OmegaCAM, the colour information and accurate model subtracted images, we have found 18 new lens candidates, for which spectroscopic confirmation will be needed to confirm their lensing nature and study the mass profile of the lensing galaxies.Comment: 4 pages, 1 figure, to appear on the refereed Proceeding of the "The Universe of Digital Sky Surveys" conference held at the INAF--OAC, Naples, on 25th-28th november 2014, to be published on Astrophysics and Space Science Proceedings, edited by Longo, Napolitano, Marconi, Paolillo, Iodic

A dedicated haem lyase is required for the maturation of a novel bacterial cytochrome c with unconventional covalent haem binding

In bacterial c-type cytochromes, the haem cofactor is covalently attached via two cysteine residues organized in a haem c-binding motif. Here, a novel octa-haem c protein, MccA, is described that contains only seven conventional haem c-binding motifs (CXXCH), in addition to several single cysteine residues and a conserved CH signature. Mass spectrometric analysis of purified MccA from Wolinella succinogenes suggests that two of the single cysteine residues are actually part of an unprecedented CX15CH sequence involved in haem c binding. Spectroscopic characterization of MccA identified an unusual high-potential haem c with a red-shifted absorption maximum, not unlike that of certain eukaryotic cytochromes c that exceptionally bind haem via only one thioether bridge. A haem lyase gene was found to be specifically required for the maturation of MccA in W. succinogenes. Equivalent haem lyase-encoding genes belonging to either the bacterial cytochrome c biogenesis system I or II are present in the vicinity of every known mccA gene suggesting a dedicated cytochrome c maturation pathway. The results necessitate reconsideration of computer-based prediction of putative haem c-binding motifs in bacterial proteomes

Perspective piece: What is a hotspot anyway?

The importance of spatial clusters, or "hotspots," in infectious disease epidemiology has been increasingly recognized, and targeting hotspots is often seen as an important component of disease-control strategies. However, the precise meaning of "hotspot" varies widely in current research and policy documents. Hotspots have been variously described as areas of elevated incidence or prevalence, higher transmission efficiency or risk, or higher probability of disease emergence. This ambiguity has led to confusion and may result in mistaken inferences regarding the best way to target interventions. We surveyed the literature on epidemiologic hotspots, examining the multitude of ways in which the term is used; and highlight the difference in the geographic scale of hotspots and the properties they are supposed to have. In response to the diversity in the term's usage, we advocate the use of more precise terms, such as "burden hotspot," "transmission hotspot," and "emergence hotspot," as well as explicit specification of the spatiotemporal scale of interest. Increased precision in terminology is needed to ensure clear and effective policies for disease control