56 research outputs found
Cell membrane damage induced by phenolic acids on wine lactic acid bacteria
The aim of this work was to investigate the effect of phenolic acids on cell membrane permeability of
lactic acid bacteria from wine. Several phenolic acids were tested for their effects on the cell membrane of
Oenococcus oeni and Lactobacillus hilgardii by measuring potassium and phosphate efflux, proton influx and
by assessing culture viability employing a fluorescence technique based on membrane integrity. The
experimental results indicate that hydroxycinnamic acids (p-coumaric, caffeic and ferulic acids) induce
greater ion leakages and higher proton influx than hydroxybenzoic acids (p-hydroxibenzoic, protocatechuic,
gallic, vanillic, and syringic acids). Among the hydroxycinnamic acids, p-coumaric acid showed the strongest
effect. Moreover, the exposure of cells to phenolic acids caused a significant decrease in cell culture viability,
as measured by the fluorescence assay, in both tested strains. The results agree with previous results
obtained in growth experiments with the same strains. Generally, phenolic acids increased the cell
membrane permeability in lactic acid bacteria from wine. The different effects of phenolic acids on
membrane permeability could be related to differences in their structure and lipophilic character
The combined use of reflectance, emissivity and elevation Aster/Terra data for tropical soil studies
Uso de ICP OES e titrimetria para a determinação de cálcio, magnésio e alumínio em amostras de solos
The upgrade of the ALICE TPC with GEMs and continuous readout
The upgrade of the ALICE TPC will allow the experiment to cope with the high interaction rates foreseen for the forthcoming Run 3 and Run 4 at the CERN LHC. In this article, we describe the design of new readout chambers and front-end electronics, which are driven by the goals of the experiment. Gas Electron Multiplier (GEM) detectors arranged in stacks containing four GEMs each, and continuous readout electronics based on the SAMPA chip, an ALICE development, are replacing the previous elements. The construction of these new elements, together with their associated quality control procedures, is explained in detail. Finally, the readout chamber and front-end electronics cards replacement, together with the commissioning of the detector prior to installation in the experimental cavern, are presented. After a nine-year period of R&D, construction, and assembly, the upgrade of the TPC was completed in 2020.publishedVersio
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