Objectively measured physical capability levels and mortality: systematic review and meta-analysis

Objective: To do a quantitative systematic review, including published and unpublished data, examining the associations between individual objective measures of physical capability (grip strength, walking speed, chair rising, and standing balance times) and mortality in community dwelling populations. Design: Systematic review and meta-analysis. Data sources: Relevant studies published by May 2009 identified through literature searches using Embase (from 1980) and Medline (from 1950) and manual searching of reference lists; unpublished results were obtained from study investigators. Study selection: Eligible observational studies were those done in community dwelling people of any age that examined the association of at least one of the specified measures of physical capability (grip strength, walking speed, chair rises, or standing balance) with mortality. Data synthesis: Effect estimates obtained were pooled by using random effects meta-analysis models with heterogeneity between studies investigated. Results: Although heterogeneity was detected, consistent evidence was found of associations between all four measures of physical capability and mortality; those people who performed less well in these tests were found to be at higher risk of all cause mortality. For example, the summary hazard ratio for mortality comparing the weakest with the strongest quarter of grip strength (14 studies, 53 476 participants) was 1.67 (95% confidence interval 1.45 to 1.93) after adjustment for age, sex, and body size (I2=84.0%, 95% confidence interval 74% to 90%; P from Q statistic <0.001). The summary hazard ratio for mortality comparing the slowest with the fastest quarter of walking speed (five studies, 14 692 participants) was 2.87 (2.22 to 3.72) (I2=25.2%, 0% to 70%; P=0.25) after similar adjustments. Whereas studies of the associations of walking speed, chair rising, and standing balance with mortality have only been done in older populations (average age over 70 years), the association of grip strength with mortality was also found in younger populations (five studies had an average age under 60 years). Conclusions: Objective measures of physical capability are predictors of all cause mortality in older community dwelling populations. Such measures may therefore provide useful tools for identifying older people at higher risk of death. With Contributions from Dr P.A. Bath, University of Sheffiel

The use of disjunct eddy sampling methods for the determination of ecosystem level fluxes of trace gases

The concept of disjunct eddy sampling (DES) for use in measuring ecosystem-level micrometeorological fluxes is re-examined. The governing equations are discussed as well as other practical considerations and guidelines concerning this sampling method as it is applied to either the disjunct eddy covariance (DEC) or disjunct eddy accumulation (DEA) techniques. A disjunct eddy sampling system was constructed that could either be combined with relatively slow sensors (response time of 2 to 40 s) to measure fluxes using DEC, or could also be used to accumulate samples in stable reservoirs for later laboratory analysis (DEA technique). Both the DEC and DEA modes of this sampler were tested against conventional eddy covariance (EC) for fluxes of either CO2 (DEC) or isoprene (DEA). Good agreement in both modes was observed relative to the EC systems. However, the uncertainty in a single DEA flux measurement was considerable (40%) due to both the reduced statistical sampling and the analytical precision of the concentration difference measurements. We have also re-investigated the effects of nonzero mean vertical wind velocity on accumulation techniques as it relates to our DEA measurements. Despite the higher uncertainty, disjunct eddy sampling can provide an alternative technique to eddy covariance for determining ecosystem-level fluxes for species where fast sensors do not currently exist

How to get started with a systematic review in epidemiology: an introductory guide for early career researchers

Background: systematic review is a powerful research tool which aims to identify and synthesize all evidence relevant to a research question. The approach taken is much like that used in a scientific experiment, with high priority given to the transparency and reproducibility of the methods used and to handling all evidence in a consistent manner.Early career researchers may find themselves in a position where they decide to undertake a systematic review, for example it may form part or all of a PhD thesis. Those with no prior experience of systematic review may need considerable support and direction getting started with such a project. Here we set out in simple terms how to get started with a systematic review.Discussion: advice is given on matters such as developing a review protocol, searching using databases and other methods, data extraction, risk of bias assessment and data synthesis including meta-analysis. Signposts to further information and useful resources are also given.Conclusion: a well-conducted systematic review benefits the scientific field by providing a summary of existing evidence and highlighting unanswered questions. For the individual, undertaking a systematic review is also a great opportunity to improve skills in critical appraisal and in synthesising evidence

New insulating phases of two-dimensional electrons in high Landau levels: observation of sharp thresholds to conduction

The intriguing re-entrant integer quantized Hall states recently discovered in high Landau levels of high-mobility 2D electron systems are found to exhibit extremely non-linear transport. At small currents these states reflect insulating behavior of the electrons in the uppermost Landau level. At larger currents, however, a discontinuous and hysteretic transition to a conducting state is observed. These phenomena, found only in very narrow magnetic field ranges, are suggestive of the depinning of a charge density wave state, but other explanations can also be constructed.Comment: 5 pages, 5 figure

Effects of Negative Energy Components in the Constituent Quark Model

Relativistic covariance requires that in the constituent quark model for mesons the positive energy states as well as the negative energy states are included. Using relativistic quasi-potential equations the contribution of the negative energy states is studied for the light and charmonium mesons. It is found that these states change the meson mass spectrum significantly but leave its global structure untouched.Comment: 14 pages revtex 3.0, 4 figures uudecoded attached in postscript format, THU-93/1

Dual-acting stapled peptides target both HIV-1 entry and assembly

Background: Previously, we reported the conversion of the 12-mer linear and cell-impermeable peptide CAI to a cell-penetrating peptide NYAD-1 by using an i,i + 4 hydrocarbon stapling technique and confirmed its binding to the C-terminal domain (CTD) of the HIV-1 capsid (CA) protein with an improved affinity (Kd ~ 1 μM) compared to CAI (Kd ~ 15 μM). NYAD-1 disrupts the formation of both immature- and mature-like virus particles in in vitro and cell-based assembly assays. In addition, it displays potent anti-HIV-1 activity in cell culture against a range of laboratory-adapted and primary HIV-1 isolates.&lt;p&gt;&lt;/p&gt; Results: In this report, we expanded the study to i,i + 7 hydrocarbon-stapled peptides to delineate their mechanism of action and antiviral activity. We identified three potent inhibitors, NYAD-36, -66 and -67, which showed strong binding to CA in NMR and isothermal titration calorimetry (ITC) studies and disrupted the formation of mature-like particles. They showed typical α-helical structures and penetrated cells; however, the cell penetration was not as efficient as observed with the i,i + 4 peptides. Unlike NYAD-1, the i,i + 7 peptides did not have any effect on virus release; however, they impaired Gag precursor processing. HIV-1 particles produced in the presence of these peptides displayed impaired infectivity. Consistent with an effect on virus entry, selection for viral resistance led to the emergence of two mutations in the gp120 subunit of the viral envelope (Env) glycoprotein, V120Q and A327P, located in the conserved region 1 (C1) and the base of the V3 loop, respectively.&lt;p&gt;&lt;/p&gt; Conclusion: The i,i + 7 stapled peptides derived from CAI unexpectedly target both CA and the V3 loop of gp120. This dual-targeted activity is dependent on their ability to penetrate cells as well as their net charge. This mechanistic revelation will be useful in further modifying these peptides as potent anti-HIV-1 agents.&lt;p&gt;&lt;/p&gt

The Onset of Anisotropic Transport of Two-Dimensional Electrons in High Landau Levels: An Isotropic-to-Nematic Liquid Crystal Phase Transition?

The recently discovered anisotropy of the longitudinal resistance of two-dimensional electrons near half filling of high Landau levels is found to persist to much higher temperatures T when a large in-plane magnetic field B|| is applied. Under these conditions we find that the longitudinal resistivity scales quasi-linearly with B||/T. These observations support the notion that the onset of anisotropy at B||=0 does not reflect the spontaneous development of charge density modulations but may instead signal an isotropic-to-nematic liquid crystal phase transition.Comment: 5 pages, 4 figure

Polynomial SUSY in Quantum Mechanics and Second Derivative Darboux Transformation

We give the classification of second-order polynomial SUSY Quantum Mechanics in one and two dimensions. The particular attention is paid to the irreducible supercharges which cannot be built by repetition of ordinary Darboux transformations. In two dimensions it is found that the binomial superalgebra leads to the dynamic symmetry generated by a central charge operator.Comment: 10 pages, LaTeX, preprint SPbU-IP-94-0

Stochastic ϕ4−\phi^4-Theory in the Strong Coupling Limit

The stochastic $\phi^4$-theory in $d-$dimensions dynamically develops domain wall structures within which the order parameter is not continuous. We develop a statistical theory for the $\phi^4$-theory driven with a random forcing which is white in time and Gaussian-correlated in space. A master equation is derived for the probability density function (PDF) of the order parameter, when the forcing correlation length is much smaller than the system size, but much larger than the typical width of the domain walls. Moreover, exact expressions for the one-point PDF and all the moments $$ are given. We then investigate the intermittency issue in the strong coupling limit, and derive the tail of the PDF of the increments $\phi(x_2) - \phi(x_1)$. The scaling laws for the structure functions of the increments are obtained through numerical simulations. It is shown that the moments of field increments defined by, $C_b=$, behave as $|x_1-x_2|^{\xi_b}$, where $\xi_b=b$ for $b\leq 1$, and $\xi_b=1$ for $b\geq1$Comment: 22 pages, 6 figures. to appear in Nuclear. Phys.

Discrete supersymmetries of the Schrodinger equation and non-local exactly solvable potentials

Using an isomorphism between Hilbert spaces $L^2$ and $\ell^{2}$ we consider Hamiltonians which have tridiagonal matrix representations (Jacobi matrices) in a discrete basis and an eigenvalue problem is reduced to solving a three term difference equation. Technique of intertwining operators is applied to creating new families of exactly solvable Jacobi matrices. It is shown that any thus obtained Jacobi matrix gives rise to a new exactly solvable non-local potential of the Schroedinger equation. We also show that the algebraic structure underlying our approach corresponds to supersymmetry. Supercharge operators acting in the space $\ell^{2}\times \ell^{2}$ are introduced which together with a matrix form of the superhamiltonian close the simplest superalgebra.Comment: 12 page