BILIPROTEINS FROM THE BUTTERFLY Pieris brassicae STUDIED BY TIME-RESOLVED FLUORESCENCE AND COHERENT ANTI-STOKES RAMAN SPECTROSCOPY

The fluorescence decay time of the biliverdin IX7 chromophore present in biliproteins isolated from Pieris brassicae is determined to be 44 ± 3 ps. This value suggests a cyclic helical chromophore structure. The vibrational frequencies determined by CARS-spectroscopy are compared with those of model compounds. The data confirm that the chromophore in the protein-bound state adopts a cyclic-helical, flexible conformation

Bacteriochlorophylls modified at position C-3

[3-Vinyl]-bacteriochlorophyll a and related pigments modified at C-3 and/or C-132 have been synthesized from bacteriochlorophyll a. The reactivity at C-3 is strongly influenced by the C-132 substituent, and vice versa. Spectroscopical data and comparison among derivatives modified at the isocyclic ring indicate that this interaction is related to formation of an intermediate enol(ate) structure. The possible role of enol(ate) formation in (bacterio)chlorophylls in nature is discussed

EPR, ENDOR, and TRIPLE resonance studies of modified bacteriochlorophyll cation radicals

A series of substituted bacteriochlorophyll molecules, all used in reconstitution experiments of reaction centers of Rhodobacter sphaeroides (Struck et al. Biochim. Biophys. Acta 1991, 1060, 262-270), were characterized by EPR, electron-nuclear double (ENDOR), and electron-nuclear-nuclear triple (TRIPLE) resonance spectroscopy in their monomeric radical cation states. Effects of different substituents at position 3 in the porphyrin macrocycle were considered, especially for two «crosslinks» between plant and bacterial chlorophylls. These are 3-vinylbacteriochlorophyll where the «bacteria» acetyl group at position 3 was substituted by vinyl and 3-acetylchlorophyll where the «plant» vinyl group was substituted by acety

A range expanding signal conditioner

Telemetry system modifications to improve signal resolution are described. Process uses zero suppression technique which consists of subtracting known voltage from input and amplifying remainder. Schematic diagram of circuit is provided and details of operation are presented

PHOTOCHEMISTRY OF PHYCOBILIPROTEINS

Native PEC from the cyanobacterium, Mastigocladus laminosus, and its isolated α-subunit show photoreversibly photochromic reactions with difference-maxima around 502 and 570 nm in the spectral region of the α-84 phycoviolobilin chromophore. (b) Native PEC and its β-subunit show little if any reversible photochemistry in the 600–620 nm region, where the phycocyanobilin chromophores on the β-subunit absorb maximally, (c) Reversible photochemistry is retained in ureadenatured PEC at pH = 7.0 or pH ≤ 3. The difference maxima are shifted to 510 and 600 nm, and the amplitudes are decreased. An irreversible absorbance increase occurs around 670 nm (pH ≤ 3). (d) The amplitude of the reversible photoreaction difference spectrum is maximum in the presence of 4–5 M urea or 1 M KSCN, conditions known to dissociate phycobiliprotein aggregates into monomers. At the same time, the phycocyanobilin chromophore(s) are bleached irreversibly, (e) The amplitude becomes very small in high aggregates, e.g. in phycobilisomes. (f) In a reciprocal manner, the phototransformation of native PEC leads to a reversible shift of its aggregation equilibrium between trimer and monomer. The latter is favored by orange, the former by green light, (g) It is concluded that the phycoviolobilin chromophore of PEC is responsible for reversible photochemistry in PEC, and that there is not only an influence of aggregation state on photochemistry, but also vice versa an effect of the status of the chromophore on aggregation state. This could constitute a primary signal in the putative function as sensory pigment, either directly, or indirectly via the release of other polypeptides, via photodynamic effects, or the like

Controlled dephasing of Andreev states in superconducting quantum point-contacts

We have studied the relaxation and dephasing processes in a superconducting quantum point contact induced by the interaction with an electromagnetic environment. Based on a density matrix approach we obtain the rates for the dissipative dynamics as function of the transmission, the phase difference on the contact and the external impedance. Our calculation allows to determine the appropriate range of parameters for the observation of coherent oscillations in the current through the contact.Comment: 8 pages, 2 figures. To appear in Physical Review

Sub-femtosecond absolute timing precision with a 10 GHz hybrid photonic-microwave oscillator

We present an optical-electronic approach to generating microwave signals with high spectral purity. By circumventing shot noise and operating near fundamental thermal limits, we demonstrate 10 GHz signals with an absolute timing jitter for a single hybrid oscillator of 420 attoseconds (1Hz - 5 GHz)