The study of professional burnout syndrome in specialists of the department of anaesthesiology and intensive care

Objective was to determine the presence and the main manifestations of professional burnout syndrome among intensive care doctors and nurses from the department of anesthesiology and intensive care. Material and methods. Resuscitators and secondary medical staff were asked to pass a questionnaire, which included a survey by V.V. Boyko «Emotional burnout», a test by C. Spielberger in the modification Y.A. Hanin, the technique of Munsterberg. Results and discussion. Professional burnout syndrome is typical for 55 % of resuscitators and 35 % of mid-level medical staff of the department of anaesthesiology and intensive care of the pediatric University clinic. As for the respondents of the first group, the presence of the «resistance» phase is characteristic, while the second group has the «resistance» and «exhaustion» phases. According to the Spielberger scale, both groups have a middle degree of situational and personal anxiety. The Munsterberg method found that selectiveness and concentration of attention before and after the daily shift is more decreased in resuscitators. Conclusion. High rates of susceptibility of specialists of the department of anaesthesiology and intensive care to the formation of professional burnout syndrome, which affects their psychological health, were established. It is necessary to develop appropriate measures to prevent this occurrence

HOST GENETICS OF SUSCEPTIBILITY Mutations in genes underlying atypical familial mycobacteriosis are not found in tuberculosis patients from Siberian populations

s u m m a r y Objectives: Atypical familial mycobacteriosis (AFM, OMIM #209950) is caused by mutations in genes regulating IL12/IFNG pathway. Some of the mutations exhibit incomplete penetrance, and they have been proposed to be involved in the common (polygenic) predisposition to tuberculosis (TB). We set out to test this hypothesis in two populations from Siberian region of Russia with high prevalence of TB. Material and methods: The prevalence of twelve mutations in IL12/IFNG pathway genes of were analysed in 331 Russians and 238 Tuvinians TB patients and in 279 healthy Russians and 265 healthy Tuvinians. A screening for new mutations and rare polymorphisms was carried out in 10 children with severe generalized TB and severe BCG-vaccine complications using Sanger's bidirectional sequencing. Results: Twelve mutations most commonly identified in AFM patients appeared to be "wild-type" monomorphic in the studied groups. No new mutations or rare polymorphisms were identified by sequencing. However, 15 common single nucleotide polymorphisms were found, none of which was associated with TB after correction for multiple testing. Conclusion: The results of the study contradict with a hypothesis that mutations underlying AFM syndrome are involved in the predisposition to TB

Оценка эффективных доз при проведении баллонной пластики пищевода у детей

Interventional examinations are associated with high levels of patient exposure per examination, which makes it particularly important to monitor individual patient doses and to assess radiation risks. The aim of this study was the assessment of effective doses for fluoroscopy-guided balloon dilatation of benign esophageal strictures, whichwasperformedonpediatricpatientsaged 1 to 2 yearsatthe Departmentof Radiosurgical Methodsof Diagnosis and Treatment of St. Petersburg State Pediatric Medical University. Patient exposure patterns were basedonourowndatacollection.    Thecalculationofeffectiveandorgandosesofpatientsnormalizedbythevalue of the dose area product measured during the study was carried out using PCXMC 2.0 software. The conversion coefficients from the dose area product to the effective dose were calculated using tissue weighting coefficients from the ICRP Publications 60 and 103 their values were 10.7 and 11.7 µSv/cGy cm2, respectively. The results of verification of the method indicated that the use of inappropriate conversion coefficients leads to an almost double underestimation of children’s effective doses. Differences in the values of conversion coefficients are significant and are explained by differences in voltage, source to image distance, and irradiation field size. A simplified model of patient exposure was proposed, which is described by a single irradiation field. The assessment of effective doses using multi-field and single-field irradiation model shows comparable results, which allows using the differentiated approach to the assessment of radiation doses of patients.Интервенционные исследования связаны с высокими уровнями облучения пациентов, что обусловливает повышенное внимание к контролю доз пациентов, а также оценки радиационных рисков и вероятности развития детерминированных эффектов. Целью данного исследования являлась оценка эффективных доз при проведении интервенционных исследований на примере баллонной пластики пищевода, которую выполняли педиатрическим пациентам в возрасте от 1 до 2 лет на базе отделения рентгенохирургических методов диагностики и лечения Санкт-Петербургского государственного педиатрического медицинского университета. Модели облучения пациентов были разработаны на основе собственных данных. Расчет эффективных и органных доз пациентов, нормированных на значение произведения дозы на площадь, измеренного в ходе исследования, проводился с помощью программного обеспечения PCXMC 2.0. Значения коэффициентов перехода от произведения дозы на площадь к эффективной дозе, определенные с использованием тканевых взвешивающих коэффициентов из Публикаций МКРЗ 60 и 103, составили 10,7 и 11,7 мкЗв/сГр х см2 соответственно. Результаты верификации метода оценки эффективных доз на примере баллонной пластики пищевода показали, что использование несоответствующих коэффициентов перехода может приводить к недооценке эффективных доз детей в несколько раз. Различия в значениях коэффициентов перехода от произведения дозы на площадь к эффективной дозе значительны и объясняются разницей в исходных параметрах используемого ангиографического аппарата, используемых параметрах напряжения, расстояния источник – приемник и размера поля облучения на самом пациенте. Для конкретной рассмотренной процедуры была предложена упрощенная модель облучения пациентов, описываемая одним полем облучения. Оценка эффективных доз с использованием многопольной и однопольной модели показала сопоставимые результаты, что позволяет в похожих случаях использовать дифференцированный подход к оценке доз облучения пациентов

Новые виды и наиболее интересные находки для флоры Урала и прилегающих территорий

The article provides information about rare and newly described species for the flora of the Urals and adjacent territories, namely: Astragalus saphronovae Kulikov, Atraphaxis decipiens Jaub. et Spach., Carex amgunensis F. Schmidt, Galium densiflorum Ledeb., Jovibarba globifera (L.) J. Parn., Najas minor All., Potamogeton nodosus Poir., Pseudorchis albida (L.) Á. Löve et D. Löve, Salix myrsinites L., Veronica agrestis L. Essays include the information on the species ecology and short taxonomic notes. © 2021 Altai State University. All rights reserved.The work was supported by the state assignments: АААА-А19-119031290052-1 “Sosudistye rasteniia Evrazii: sistematika, flora, rastitelʹnye resursy” [“Vascular plants of Eurasia: systematics, flora, plant resources”], АААА-А18-118011990151-7 “Izucheniye, sokhraneniye i rasshireniye bioraznoobraziya rasteniy v prirode i pri introduktsii na Yuzh nom Urale i Priuralye” [“Study, conservation and expansion of plant biodiversity in nature and during introduction in the Southern Urals and the Cis- and Trans-Urals region”], AAAA-A17-117050400146-5 “Otsenka prostranstvennovremennoy izmenchivosti bioraznoobraziya i usloviy yego formirovaniya na Severe Zapadnoy Sibiri v svyazi s izmeneniyem klimata i osvoyeniyem neftegazovykh resursov” [“Assessment of the spatio-temporal variability of biodiversity and the conditions for its formation in the north of Western Siberia in connection with climate change and the development of oil and gas resources”], and by “Programmoy povysheniya konkurentosposobnosti Uralskogo federalnogo universiteta” [“The program for improving the competitiveness of the Ural Federal University”] (the decree no. 211 of the Government of the Russian Federation, contract no. 02.A03.21.0006)

Adult onset asthma and interaction between genes and active tobacco smoking: The GABRIEL consortium.

BACKGROUND: Genome-wide association studies have identified novel genetic associations for asthma, but without taking into account the role of active tobacco smoking. This study aimed to identify novel genes that interact with ever active tobacco smoking in adult onset asthma. METHODS: We performed a genome-wide interaction analysis in six studies participating in the GABRIEL consortium following two meta-analyses approaches based on 1) the overall interaction effect and 2) the genetic effect in subjects with and without smoking exposure. We performed a discovery meta-analysis including 4,057 subjects of European descent and replicated our findings in an independent cohort (LifeLines Cohort Study), including 12,475 subjects. RESULTS: First approach: 50 SNPs were selected based on an overall interaction effect at p<10-4. The most pronounced interaction effect was observed for rs9969775 on chromosome 9 (discovery meta-analysis: ORint = 0.50, p = 7.63*10-5, replication: ORint = 0.65, p = 0.02). Second approach: 35 SNPs were selected based on the overall genetic effect in exposed subjects (p <10-4). The most pronounced genetic effect was observed for rs5011804 on chromosome 12 (discovery meta-analysis ORint = 1.50, p = 1.21*10-4; replication: ORint = 1.40, p = 0.03). CONCLUSIONS: Using two genome-wide interaction approaches, we identified novel polymorphisms in non-annotated intergenic regions on chromosomes 9 and 12, that showed suggestive evidence for interaction with active tobacco smoking in the onset of adult asthma

Полиморфизм С-703Т-гена интерлейкина-5 и маркеры эозинофильного воспаления у больных бронхиальной астмой и их родственников

Markers of eosinophilic inflammation were studied in bronchial asthma patients and their relatives (bronchial hyperreactivity, eosinophils of nasal smear, total serum immunoglobulin E, interleukin-5). An association of C-703T interleukin-5 gene polymorphism and the disease was analysed. Results of our study showed a close relationship between eosinophils in nasal smear, total serum immunoglobulin E and bronchial hyperreactivity in mechanisms involved in asthma and atopy development. The statistically significant association of C-703T interleukin-5 gene polymorphism with asthma was shown.В выборке больных атопической бронхиальной астмой и членов их семей исследованы маркеры эозинофильного воспаления (бронхиальная гиперреактивность, эозинофилы в мазках-отпечатках со слизистой носа, общий иммуноглобулин Е и интерлейкин-5 в сыворотке крови) и проведен анализ ассоциации полиморфизма С-703Т-гена интерлейкина-5 с заболеванием. Показана взаимосвязь изученных маркеров эозинофильного воспаления в механизмах развития астмы и атопии. Установлена достоверная ассоциация полиморфизма С-703Т-гена интерлейкина-5 с бронхиальной астмой

Актуализация формы федерального государственного статистического наблюдения № 3-ДОЗ «Сведения o дозах облучения пациентов при проведении медицинских рентгенорадиологических исследований». Предпосылки к переработке

Analysis of levels of exposure of public of the Russian Federation by sources of ionizing exposure is one of main parts of the activities conducted by the Federal Service for Surveillance on Consumer Rights Protection and Human Wellbeing. Data on the doses of public of the Russian Federation from medical exposure on facility, regional and federal levels is collected using the form of federal governmental statistical surveillance № 3-DOZ “Data on patient doses from medical X-ray examinations”, that is active since 2000. For the last 20 years there were no significant updates of the form № 3-DOZ. According to the decision of the Board of the Rospotrebnadzor from 11.09.2020, a complex program on update and modernization of form № 3-DOZ was initiated, that has resulted in the approvement of new form № 3-DOZ by the order of Rosstat № 880. The form has been significantly changed to reflect the modern condition of X-ray diagnostics in the Russian Federation. The aim of the current study was to analyze main components of medical exposure dose data collection system that required update and modernization. The study was performed based on the results of assessment of the forms № 3-DOZ from different medical facilities and regions in 2015-2020. The results of the study allowed developing main approaches to the update of the form № 3-DOZ that were implemented in a new edition of the form.Анализ уровней облучения населения Российской Федерации источниками ионизирующего излучения является важной частью мероприятий, проводимых Федеральной службой по надзору в сфере защиты прав потребителей и благополучия человека. Информация о дозах облучения населения Российской Федерации при медицинском облучении на объектовом, региональном и федеральном уровнях собирается с использованием формы федерального статистического наблюдения № 3-ДОЗ «Сведения о дозах облучения пациентов при проведении медицинских рентгенорадиологических исследований», действующей с 2000 г. За 20 лет использования формы № 3-ДОЗ она существенно не модернизировалась. В рамках решения коллегии Роспотребнадзора от 11.09.2020 г. проводилась комплексная программа по актуализации и модернизации формы № 3-ДОЗ, которая завершилась утверждением новой формы № 3-ДОЗ приказом Росстата № 880 от 30 ноября 2022 г., при этом форма № 3-ДОЗ претерпела значительные изменения, направленные на учет современного состояния лучевой диагностики в Российской Федерации. Целью данной работы являлся анализ основных компонентов системы сбора данных по уровням медицинского облучения, которые нуждались в доработке и актуализации в рамках переработки формы № 3-ДОЗ. Работа была выполнена с учетом результатов анализа объектовых и региональных форм № 3-ДОЗ за период 2015–2020 гг. По результатам работы были сформированы основные направления актуализации формы № 3-ДОЗ, реализованные в новом варианте формы

Meta-analysis identifies seven susceptibility loci involved in the atopic March

Eczema often precedes the development of asthma in a disease course called the a 'atopic march'. To unravel the genes underlying this characteristic pattern of allergic disease, we conduct a multi-stage genome-wide association study on infantile eczema followed by childhood asthma in 12 populations including 2,428 cases and 17,034 controls. Here we report two novel loci specific for the combined eczema plus asthma phenotype, which are associated with allergic disease for the first time; rs9357733 located in EFHC1 on chromosome 6p12.3 (OR 1.27; P=2.1 × 10 a'8) and rs993226 between TMTC2 and SLC6A15 on chromosome 12q21.3 (OR 1.58; P=5.3 × 10 a'9). Additional susceptibility loci identified

Structure and absorption spectra of substituted 2-hydroxy-2-trifluoromethylchroman-4-ones

UV absorption spectra of substituted 2-hydroxy-2-trifluoromethylchroman-4-ones (chromanones) in aqueous ethyleneglycol were studied. Replacement of hydrogen in the aromatic ring of the chromanones by CH3 or CF3 groups was shown to cause the molar extinction coefficients of the K, B, and C=O bands to shift from 16,160 to 27,980 and 30,250 dm3/mol·cm; from 12,750 to 17,920 and 16,130 dm3/mol·cm; and from 3850 to 3630 and 2760 dm3/mol·cm, respectively. Increasing the solution pH from 1 to 10.5 affected weakly the aromatic ring bands yet impacted greatly the chromanone carbonyl bands, causing their λmax to shift from 311-326 nm to 342-346 nm and their extinction coefficients to increase from 2760-3850 dm3/mol·cm to 9940-10,160 dm3/mol·cm. A further rise in the pH resulted in hypochromia of the carbonyl band due to the destruction of the chromanones. The results were confirmed by NMR spectroscopy and GC methods. © 2012 Springer Science+Business Media New York