A generalized framework towards structural mechanics of three-layered composite structures

Three-layered composite structures find a broad application. Increasingly, composites are being used whose layer thicknesses and material properties diverge strongly. In the perspective of structural mechanics, classical approaches to analysis fail at such extraordinary composites. Therefore, emphasis of the present approach is on arbitrary transverse shear rigidities and structural thicknesses of the individual layers. Therewith we employ a layer-wise approach for multiple (quasi-) homogeneous layers. Every layer is considered separately whereby this disquisition is based on the direct approach for deformable directed surfaces. We limit our considerations to geometrical and physical linearity. In this simple and familiar setting we furnish a layer-wise theory by introducing constraints at interfaces to couple the layers. Hereby we restrict our concern to surfaces where all material points per surface are coplanar and all surfaces are plane parallel. Closed-form solutions of the governing equations enforce a narrow frame since they are strongly restrictive in the context of available boundary conditions. Thus a computational solution approach is introduced using the finite element method. In order to determine the required spatially approximated equation of motion, the principle of virtual work is exploited. The discretization is realized via quadrilateral elements with quadratic shape functions. Hereby we introduce an approach where nine degrees of freedom per node are used. In combination with the numerical solution approach, this layer-wise theory has emerged as a powerful tool to analyze composite structures. In present treatise, we would like to clarify the broad scope of this approach

On the Contributions of Pavel Andreevich Zhilin to Mechanics

This paper is dedicated to the memory of Pavel A. Zhilin (1942-2005), the great Russian scientist in the field of Rational Mechanics. He was educated and worked at the State Polytechnical University in St. Petersburg (Russian Federation), formerly known as the Polytechnical Institute. As Head of the Department of Theoretical Mechanics he supervised sixteen PhD theses (Candidate of Science theses) and six higher doctorates (Habilitations or Doctor of Science theses), some of them are shown on Fig. 2. His scientific interests covered various branches of Mechanics and Theoretical Physics. In his research he strived to pave a way based on Rational Mechanics to areas which are traditionally not associated with Mechanics, such as Physics of Microstructures and Electrodynamics. The paper gives a brief summary of the scientific biography and the main results obtained by Pavel A. Zhilin1

Response of deep-sea benthic foraminifera to a simulated sedimentation event

The response of deep-sea benthic foraminifera to a simulated sedimentation event was assessed in a shipboard microcosm by using transmission electron microscopy, organic carbon, adenosine nucleotide, ETS assays and live observations. A rapid activation of foraminiferal metabolism was detected with an increase in ETS activity and a distinct decrease in AMP to build up A TP. Based on this phy iological-awakening reaction, food was gathered by pseudopodia] activity and ingested in high quantities. Three days after feeding, high quality food was found in the digestion vacuoles leading to an increase of 89% in individual organic carbon contents. During the following six days, this food was converted into biomass as lipids and other reserve compounds were built up. After ten days, digestion vacuoles contained degraded matter onJy. The final shift in individual biomass was 16.9% in comparison to the initial population

Natural and human-induced Holocene paleoenvironmental changes on the Guadiana shelf (northern Gulf of Cadiz)

Three contrasting sedimentary environments on the continental shelf off the Guadiana River (northern Gulf of Cadiz) were integrated in a chronological framework and analysed in terms of sedimentology and benthic foraminiferal assemblages to understand the Holocene paleoenvironmental evolution. The analysed environments differ in terms of their depositional regimes and benthic foraminiferal assemblages. However, a dominant fluvial origin of the sand fraction was observed in all three environments. Holocene sedimentary processes were mainly controlled by natural (sea level changes and climate variations) and human-induced processes (e.g. deforestation, agriculture) along four evolutionary stages. The three older stages were mainly influenced by natural processes, such as sea level variations and fluvial inputs, whereas the most recent stage reflects a combination of climatic- and human-induced processes. A deepening of sedimentary environments related to a period of rapid sea level rise, strongly influenced by river discharges occurred from c. 11,500 to c. 10,000 cal. yr BP. A reduction in sediment export to the shelf, as a result of the continuous and rapid sea level rise and enhanced estuary infilling reflects the second stage, from c. 10,000 to c. 5000 cal. yr BP. The beginning of the third stage, from c. 5000 to c. 1500–1000 cal. yr BP, is marked by a sea-level slowdown and the relatively stable climate and environmental conditions. The fourth stage, from c. 1500–1000 cal. yr BP to Recent times, reflects the intensification of human-induced processes and climatic variability in the Guadiana River basin. This stage also reflects modern depositional conditions, with the formation of a proximal prodeltaic wedge and a distal muddy body

The Roles of Transmembrane Domain Helix-III during Rhodopsin Photoactivation

Background: Rhodopsin, the prototypic member of G protein-coupled receptors (GPCRs), undergoes isomerization of 11- cis-retinal to all-trans-retinal upon photoactivation. Although the basic mechanism by which rhodopsin is activated is well understood, the roles of whole transmembrane (TM) helix-III during rhodopsin photoactivation in detail are not completely clear. Principal Findings: We herein use single-cysteine mutagenesis technique to investigate conformational changes in TM helices of rhodopsin upon photoactivation. Specifically, we study changes in accessibility and reactivity of cysteine residues introduced into the TM helix-III of rhodopsin. Twenty-eight single-cysteine mutants of rhodopsin (P107C-R135C) were prepared after substitution of all natural cysteine residues (C140/C167/C185/C222/C264/C316) by alanine. The cysteine mutants were expressed in COS-1 cells and rhodopsin was purified after regeneration with 11-cis-retinal. Cysteine accessibility in these mutants was monitored by reaction with 4, 49-dithiodipyridine (4-PDS) in the dark and after illumination. Most of the mutants except for T108C, G109C, E113C, I133C, and R135C showed no reaction in the dark. Wide variation in reactivity was observed among cysteines at different positions in the sequence 108–135 after photoactivation. In particular, cysteines at position 115, 119, 121, 129, 131, 132, and 135, facing 11-cis-retinal, reacted with 4-PDS faster than neighboring amino acids. The different reaction rates of mutants with 4-PDS after photoactivation suggest that the amino acids in different positions in helix-III are exposed to aqueous environment to varying degrees. Significance: Accessibility data indicate that an aqueous/hydrophobic boundary in helix-III is near G109 and I133. The lack of reactivity in the dark and the accessibility of cysteine after photoactivation indicate an increase of water/4-PDS accessibility for certain cysteine-mutants at Helix-III during formation of Meta II. We conclude that photoactivation resulted in water-accessible at the chromophore-facing residues of Helix-III.National Institutes of Health (U.S.) (grant GM28289)National Eye Institute (Grant Grant EY11716)National Science Foundation (U.S.) (grant EIA-0225609