Perbandingan Metode Penetapan Kadar Simetidin Menggunakan Spektrofotometri Uv Dan Kromatografi Cair Kinerja Tinggi

Determination ofdrug substance is one of the drug quality control to ensure the safetyof drug. Determination of cimetidine could use UV spectrophotometry and HPLC. The aim ofthis study were to validate the methode using two instruments, to compare both of the method and to apply it intablet dosage forms. Determination of cimetidin using UV spectrophotometry was set on wave length 219 nm. Determination using HPLC with coloumn C18 and mobile phase mixture of methanol: water-phosphoric acid (30:70, v/v), flow rateat 1mL/min and UV detector. The method was validated in terms of accuracy, precision, linearity, selectivity and sensivity. The method of determination of cimetidine using both instruments were campared by analysis of variant. Validation method using UV spectrophotometry showed precision of 0.94%, recovery from 97.50 to 100.91%, good linearity, LOD 0.76 µg/mL and LOQ 2.52µg/mL.Validation using HPLC method resulted value of precision 0.30%, recovery from 98.42 to 101.83%, good linearity, LOD and LOQ of 0.46 and 0.56 µg/mL. The average concentration of cimetidine in tablets by spectrophotometry UV was 101.95%, while in HPLC was 99.69%.The methods accomplied to the requirements according The Indonesian Pharmacopoeia Edition IV. Both methods provided the same results and did not significantly different

Perbandingan Kadar Sukrosa Dalam Madu Randu Dan Madu Kelengkeng Dari Peternak Lebah Dan Madu Perdagangan Di Kota Semarang

Key words: cottonwoods honey, longan honey, sucrose, KCKT Honey is a natural liquid that generally has a sweet taste and produced by the bees. Along with the increase in consumption of honey, honey adulteration grew a way to get the benefits. Sucrose sugar often added because its price is relative cheap. The content of sucrose become a measurement of the authenticity of honey. The spurious honey harm consumers because of different composition so that its benefits are also different.The purpose of this research is to determine the sucrose content and the quantitation of sucrose in longan honey and honey cottonwoods from beekeepers and honey trade in the City of Semarang using HPLC.Honey samples were taken randomized with three sample of cottonwoods and three longan honey from beekeepers area in Ngaliyan, Gringsing dan Ambarawa and honey trade in the City of Semarang. The determination of sucrose using HPLC system (Waters ec 2695) equipped with C18 column (C18 5um Sunfire; 4.6 x150mm) and detector UV (UV/Visible Waters 2489) at wavelength 190 nm. Mobile phase consisted of a mixture of acetonitrile and aquabides (75:25, v/v) with a flow rate of 1.0 mL/min.Research results showed that the average content of sucrose in honey samples cottonwoods and longan honey from beekeepers respectively 4,03% dan 2,98%, while the average level for the sample of cottonwoods and longan honey trade in the City of Semarang respectively 2,11% and 2,59%. The average level of sucrose from twelve samples meet requirements but the there are two samples that does not comply requirement of honey based on SNI-2004. There was no significant difference between the levels of sucrose honey cottonwoods and honey longan from beekeepers and honey trade in the City of Semarang

Uji Aktivitas Antibakteri Ekstrak Etanol Daun Jarak Pagar (Jatropha Curcas L) Terhadap Bakteri Staphylococcus Aureus Atcc 25923, Escherichia Coli Atcc 25922, Dan Salmonella Typhi Atcc 1408

Jatropha curcas L is a plant species that can be used for curing, since it contains flavonoid, saponin, and tanin. Jatropha multifida L is proved to have antibacterial activities (Sisunandar, et.al., 2002). This research was intended to find out the antibacterial activities of ethanol extracts of Jatropha curcas L leaves on bacteria Staphylococus aureus, Escherichia coli, and Salmonella typhi, find out the effectiveness of those antibacterial activities and identify the compound groups contained in the ethanol extracts, the result of antibacterial activity of ethanol extracts of Jatropha curcas L was analized by statistic. Employing a maceration method, Jatropha curcas L leaves were extracted using ethanol 70% for five days. Employing an agar-diffusion method aided by disk papers and in order to find out the inhibitory area diameters of the extracts, the extracts were then tested for their antibacterial activities. Qualitative analysis on the chemical contents of the extracts were conducted employing. TLC method aided by silica-gel stationary phases for saponin and tanin and cellulose phases for flavonoid. The motion phases used ethyl acetat-acidformiat-acid acetat-water for flavonoid, chloroform-methanol for saponin and buthanol-acid acetat-water for tanin. The result of the test on antibacterial activities were analyzed statistically using Kruskall Wallis and Mann & Whitney tests. The analysis showed that ethanol extracts of the Jatropha curcas L leaves inhibited the growth of Staphylococcus aureus but not the growth of Eschericia coli and Salmonella typhi. The testing concentrations of 20%, 40%, 60%, 80%, and 100% b/v produed inhibitory area diameters averaging 8.25, 9.25, 11.00, 13.25, and 19.00 mm respectively. The positive control of Ampicillin produced an average inhibitory area diameters of 40.00 mm while the solvent control did not produce any inhibitory area diameters. Statistical test showed that there were significant differences among the different concentrations of the extracts.Thin Layer Chromatography tests produced yellow color showing the existence of flavonoid, bluish violet color showing the existence of saponin, and grayish green color showing the existence of tanin

Uji Kandungan Bakteri Escherichia Coli Pada Air Minum Isi Ulang Dari Depot Air Minum Isi Ulang Di Kabupaten Rembang Testing of Escherichia Coli Bacteria Content in Drinking Water Refill From Drinking Water Refill Depot in Rembang Sub-district

Water refill is water that has through purification processes both Ultraviolet and ionization, by many stages of filtration to obtain clean water, in order to provide human needs. This research performed by Athena, et.al., (2003) to shows that Total Coli and Escherichia coli in high-enough amounts inside water refill from water refill depot (DAMIU) in Jakarta, Tangerang, and Bekasi. There are many efforts related with this water refill that causes DAMIU developed rapidly, so that required monitoring improvement because water is the primary needs of human being. This research aims to identify Escherichia coli bacteria content that exist in drinking water of water refill from DAMIU in Rembang Sub district. This research is survey type, equipped with microbiology test using MPN method (Most Probable Number). The populations are 25 DAMIU in Rembang sub-district. Data analysis performed descriptively. Test, conducted by water refill sampling, which produced by DAMIU in Rembang sub-district. The testing includes: approximation test using Lactosa Broth (LB) medium, confirmation test using Briliant Green Lactose Bile Broth (BGLB) medium, complementary test using Mc. Conkey medium and Gram painting was performed to identify bacteria types using microscope by 100 times magnification, and Biochemistry test that is IMVIC (Indol, Methyl-Red, Voges Proskauer, and Citrat). In water refill which produced by DAMIU in Rembang Sub district, there is 1 sample (4%) with MPN value of E. coli 13/100 ml and Coliform MPN 21/100 ml, does not fulfill the bacteriology requirements of drinking water quality, according to Health Minister Decree No.907/MENKES/SK/VII/2002 : ³The existence of E.Coli bacteria 0/100 ml sample´, while 24 samples has fulfill the requirements, with MPN value of E.Coli and Coliform < 2, so that it safe and ready to consume

Evaluasi Kandungan Vitamin Cdalam Madu Randu Dan Madu Kelengkeng Dari Peternak Lebah Dan Madu Perdagangan Di Kota Semarang

Key words: vitamin C, cottonwoods honey, longan honey, HPLC Honey is a natural product that containing several vitamins, including vitamin C, which has an important role to maintain health, among others helps healing wound, increase body resistance fight infection and as antioxidant. This research aimed to determine the levels of vitamin C in the cottonwoods honey and longan honey from beekeepers and honey trade in the city of Semarang using HPLC.Samples consisted of three samples of honey and three cottonwoods honey and longan honey from beekeepers different (Ambarawa, Ngaliyan, Gringsing) and honey trade in the city of Semarang. HPLC (waters ec 2695) operational conditions is equipped stationary phase C18 column (C18 5um sunfire; 4.6 X150 mm), and the mobile phase is a mixture of phosphate buffer pH 3.6 and acetonitrile with the ratio 70: 30, v / v, flow rate of 0.5 ml / min. Detection use UV detector (UV / vis water in 2489) at 260 nm wavelength.Research results show ed that the average levels of vitamin C in the cottonwoods honey from beekeepers and honey cottonwoods trade in the city of Semarang are 0,034% and 0,016%, while for longan honey respectively 0,027% and 0,032%. The average level of the twelve samples of honey contain vitamin C, but there are two samples that does not contain vitamin C. There was no significant difference between the levels of vitamin C and cottonwoods honey and longan honey from beekeepers and honey trade in the City of Semarang

Potensi Daur Ulang dan Partisipasi Masyarakat dalam Pengelolaan Sampah di Kecamatan Jabon, Kabupaten Sidoarjo

Tujuan penelitian ini adalah menentukan potensi timbulan sampah organik, potensi daur ulang dan menentukan rekomendasi untuk meningkatkan partisipasi masyarakat dalam pengelolaan sampah di Kecamatan Jabon. Penelitian ini dilakukan pada 2 aspek yaitu aspek teknis dan aspek masyarakat. Untuk aspek teknis melakukan sampling timbulan, komposisi dan densitas sampah rumah tangga, sentra industri tempe dan kerupuk, peternakan ayam dan bebek. Sedangkan untuk aspek masyarakat menyebarkan kuisioner yang berisikan kategori pengetahuan, sikap dan perilaku yang kemudian akan diolah dengan metode likert scale dan chi kuadrat. Jumlah responden rumah tangga berjumlah 100 KK yang tersebar di 3 desa. Rata-rata timbulan sampah yang dihasilkan untuk sampah rumah tangga adalah 0,27 kg/org/hari, sentra industri tempe 28.639,16 kg/hari, sentra industri kerupuk 9.766,83 kg/hari, peternakan ayam 166,70 kg/hari dan peternakan bebek 630,15 kg/hari

Production Performance of Debu and Kelabu Sentul Chicken at Different Igy Concentrations

Sentul chicken is one of Indonesia’s native chickens that has high potential for meat and egg production performance. The production of Sentul chicken breeds with high body resistance is one of the solutions to fulfill this potential. This research aimed to ascertain the impact of various IgY concentrations (high, medium, and low) on the production of 90 Debu and 90 Kelabu Sentul chickens. Parameters observed included pre-laying and laying performance. Six replications of a completely randomized design were used to conduct the experiment. The Anova test was used to analyze the data that was collected. The findings revealed that different strains and sex of Debu and Kelabu Sentul chickens did not significantly affect the value of IgY concentration. In the entire population studied, only 12.09% of Debu Sentul chickens and 11. 32% of Kelabu Sentul chickens had low Igy concentration values. In pre-laying period, the difference in the types of chicken strains has no significant effect on the body weight gain and thefeed convertion. Chickens with high IgY concentrations performed worse during the laying phase than hens with low IgY concentrations. The research found that the population of Kelabu Sentul chickens with high IgY concentration value is more than Debu Sentul chickens, which makes them suitable to be used as breeders

Pengaruh Bauran Pemasaran terhadap Keputusan Konsumen dalam Pembelian Pestisida di Ud. Agrimart Sejahtera Kota Pangkalan Kerinci Kabupaten Pelalawan

This research aims to: (1). Determine the marketing mix that influence the consumers' decisions in purchasing pesticides at UD. Agrimart Sejahtera in Pangkalan Kerinci. (2). Determine the constraints faced by UD. Agrimart Sejahtera in marketing the pesticides products. In this research, 50 respondents has been selected through purposive sampling that would be interviewed, then analyzed descriptively and used likert scale. To answer the first goal, it has been analyzed descriptively and used likert scale. To answer the second goal, it has been done through interview at UD. Agrimart Sejahtera in Pangkalan Kerinci. The result of the research shows: (1). Overall the consumers' decision in purchasing the pesticides at UD. Agrimart Sejahtera got an average value 195.12 that was included in “agree” categories, the consumers were agree with the marketing mix exsisted at UD. Agrimart Sejahtera with variable products, prices, places, promotions and services. (2). The constraints faced by UD. Agrimart Sejahtera: (a). Constraints in limiting some pesticides product brand from several distributors. (b). Price fluctuations constraints from distributors. (c). Promotion constraints

Pemanfaatan Tempe dengan Jamur Tiram (Pleurotus Ostreatus) dalam Pembuatan Nugget

The purpose of this study was to obtain the best nugget formulation of utilization tempeh and oyster mushrooms both in terms of organoleptic and nutritional substances nuggets. This research used Complete Randomized Design (CRD) with four treatments and four replications. The treatments were TJ1 (95% Tempe, 5% oyster mushroom), TJ2 (90% Tempe, 10% oyster mushroom), TJ3 (85% Tempe, 15% oyster mushroom), TJ4 (80% Tempe, 20% oyster mushroom). The data obtained were analyzed using analysis of variance followed by Duncan's New Multiple Range Test (DNMRT) at 5% level. Results showed that the ratio of tempeh and oyster mushroom in each treatment significantly affected the moisture content, ash content, fiber content, protein content and flavor, but did not significantly affect the elasticity, adhesiveness, aroma, colour and overall assessment. The best treatment in this study is TJ1 (95% Tempe, 5% oyster mushroom) with a moisture content 46,62%, protein content 16,29%, ash content 0,47% and fiber content 3,47%