4 research outputs found
ASSOCIATION BETWEEN TOXOPLASMA GONDII INFECTION IN WOMEN AND THE PRESENCE OF CARDIOLIPIN AND PHOSPHOLIPID ANTIBODIES
IgG and IgM cardiolipin and phospholipid antibodies were measured, by an ELISA technique, in the sera of patients with toxoplasmosis. Immunological methods for autoantibodies had been applied by ELISA. Regarding Anti-cardiolipin it was revealed that 24.7% of toxoplasmosis cases versus 0% of control group showed significant positive result (P=0.0412) . Whereas, 12.5% of toxoplasmosis patients and 12% of healthy controls had anti-cardiolipin antibodies, with no significant differences (P=0.554) between these two groups
Tracking of Ceotaneous Leishmaniasis by Parasitological, Molecular and Biochemical Analysis
Abstract:Background: In areas of endemicity without sufficient laboratory infrastructure, identification of Leishmaniaparasites is useful for control and preventive plan and very important since species differentiation is instrumental in selection of optimal therapy and treatment regimens.Aim: The present study was performed to identify the species and strain of Leishmaniaparasiteisolated from different endemic areas .Materials and Methods: In a consecutive series of 104 patients referred for a suspected CL lesion during October 0212 to December 0210 in five Iraqi provinces, direct smear and culture followed by molecular and biochemical analysis were done using nested-PCR and cellulose acetate electrophoresis
Evaluation of Matrix Metalloproteinase-2 (MMP2) in Aborted Women Infected with Toxoplasma gondii.
AbstractBackground: Toxoplasmosis is one of the most common zoonotic pathogens worldwide; Life cycle of this parasite was affected by synthesis of specific enzyme like Matrix metalloproteinase-2 (MMP2).Objectives: The major objectives of this study detection of prevalence expression of Matrix metalloproteinase-2 (MMP2) among ninety-six aborted women were tested; seventy-six of them was with recurrent abortions due to Toxoplasmosis and twenty without Toxoplasmosis as control group in Najaf governorate.Methodology: Descriptive case-control ,purposive (probability) sample among ninety six aborted women were tested, attending in Central Health Laboratory, Al-Zahra Maternity&paedriatic Teaching Hospital , The data collected from January 2014 to January 2015. The concentration level of Matrix metalloproteinase-2 (MMP2) in aborted women was measured by ELISA technique according to many parameters such as age, occupation, residence and number of abortions.Results: The result indicated that the concentration of MMP2was elevated in women with one or two abortions. The high concentration of MMP2 in infected aborted women with age group (20-24), (25-29).there was significant differences in study groups according to occupation and there were differences in MMP2concentration levels according to residency. It was demonstrated that the high concentration of MMP2 was appear in rural infected aborted women and low in urban women with Toxoplasmosis, Results were analyzed by using statistical methods (Levene test, ANOVA test, t test).Conclusion: Matrix metalloproteinase-2(MMP2) play a significant role in immunity against infection with Toxoplasma gondii.Recommendation: Further studies are recommended to know the concentration of MMP2 among pregnant women to be one of an important laboratory tests in the induction of immune response against this type of infection.Keywords: Matrix metalloproteinase-2 (MMP2), Toxoplasma gondii, aborted women
A comparative study of effectiveness of diagnostic tests (r K39 strip, IFAT and bone marrow stained smear) for visceral leishmaniasis.
Background: Iraq is an endemic area for visceral leishmaniasis (VK=L) (kala-azar) which is caused by the intracellular parasite leishmania donovani