Abstract:Background: In areas of endemicity without sufficient laboratory infrastructure, identification of Leishmaniaparasites is useful for control and preventive plan and very important since species differentiation is instrumental in selection of optimal therapy and treatment regimens.Aim: The present study was performed to identify the species and strain of Leishmaniaparasiteisolated from different endemic areas .Materials and Methods: In a consecutive series of 104 patients referred for a suspected CL lesion during October 0212 to December 0210 in five Iraqi provinces, direct smear and culture followed by molecular and biochemical analysis were done using nested-PCR and cellulose acetate electrophoresis
