Atypical Protein Kinase Cs in Melanoma Progression

Melanoma is one of the fastest growing types of cancer worldwide in terms of incidence. To date, reports show over 92,000 new cases in the United States in 2018. Previously, we introduced protein kinase C-iota (PKC-ι) as an oncogene in melanoma. PKC-ι promotes survival and cancer progression along with PKC-zeta(ζ). In addition, we reported that PKC-ι induced metastasis of melanoma cells by increasing Vimentin dynamics. Our previous results showed that PKC-ι inhibition downregulated epithelial-mesenchymal transition (EMT), while inducing apoptosis. In this chapter, we summarized these findings which were based on the in-vitro applications of five specific atypical PKC (aPKC) inhibitors. In addition, the underlying mechanisms of the transcriptional regulation of PRKCI gene expression in melanoma is also discussed. Results demonstrated that c-Jun promotes PRKCI expression along with Interleukin (IL)-6/8. Furthermore, forkhead box protein O1 (FOXO1) acts as a downregulator of PRKCI expression upon stimulation of IL-17E and intercellular adhesion molecule 1 (ICAM-1) in melanoma cells. Overall, the chapter summarizes the importance of PKC-ι/ζ in the progression of melanoma and discusses the cellular signaling pathways that are altered upon inhibitor applications. Finally, we established that aPKCs are effective novel biomarkers for use in the design of novel targeted therapeutics for melanoma

Development of new cultivation technology for straw mushroom (Volvariella volvacea) using locally available raw materials

Paddy straw mushroom (Volvariel1a volvacea) is an edible mushroom variety which can be cultivatedunder tropical and sub tropical conditions. In Sri Lanka, though the majority of farmers grow oystermushroom they are willing to undertake other mushroom types, including straw mushroom and milkymushroom. Straw mushroom cultivation is highly rewarding because of the favourable climaticconditions in Sri Lanka and the abundant availability of raw materials. The existing outdoor methodfor straw mushroom cultivation introduced by the Department of Agriculture (DOA) gives low orlandirregular yield. As the optimum environmental conditions are crucial in straw mushroom production,an indoor cultivation method using a polythcne house was tested with the existing outdoor method.Cotton waste and paddy straw were used as the growing media in both outdoor and indoor conditionsunder four treatments viz. paddy straw compost in polythene house (T 1), cotton waste compost inpolythene house (T2), paddy straw in outdoor environment (T3) and cotton waste in outdoor environment(T4), arranged in a Completely Randomized Design with three replicates. The results revealed that the indoor cultivation method with cotton waste compost substrate (TI) gave significantly highervalues for average yield (6901.\8 kg/ha) and average marketable yield (6489 kg/ha) compared toother treatments. Outdoor culture in straw substrate (T3) resulted lowest values for the same yieldparameters (567.\3 kg/ha, 516.3\ kg/ha, respectively). Indoor cultivation method with paddy strawcompost and cotton waste compost both resulted higher yields when compared to the outdoor cu Iture.As paddy straw is freely available in Sri Lanka, combining of paddy straw compost and cotton wastecompost as the substrate for straw mushroom culture under indoor conditions would be more profitabl

COMPARISON OF FLORISTIC DIVERSITY OF FOUR WOODLAND TYPES IN THE UPPER HANTANA CAMPUS LAND

In the Upper Hantana campus land, three broad-leaf woodlands Peresereanthes fa/cataria.A /stonia macrophylla, or mixed species woodland and Pinus caribaea woodland grow inproximity to one another. The wide range of floristic diversity exhibited by these differentwoodlands was compared using plot sampling.The overstorey vegetation of the Alstonia woodland showed the highest, i) density, ii)floristic richness, iii) proportion of endemics and iv) plant diversity, foIlowed in decreasingorder by that in the Peresereanthes-, mixed-, and Pinus woodland. In the understoreyvegetation, floristic diversity «10 ern) was highest in Peresereanthes woodland, foIlowedin decreasing order by that in Alstonia-, mixed species-, and Pinus woodlands. Speciesrank abundance plots of overstorey and understorey species show that over storeyvegetation in Pinus woodland, fits the geometric series, whereas the others fit the logseries or log normal model.The broad-leaf species woodlands in Upper Hantana also showed better naturalregeneration of an array of species. AIl woodlands showed differences in micro-siteconditions. Levels of human disturbance and burning may contribute to differencesamongst them. These results provide baseline ecological information on naturalregeneration in different woodland types and indicate their relative potential forconservation of biodiversity and water resources.

Impact of culture towards disaster risk reduction

Number of natural disasters has risen sharply worldwide making the risk of disasters a global concern. These disasters have created significant losses and damages to humans, economy and society. Despite the losses and damages created by disasters, some individuals and communities do not attached much significance to natural disasters. Risk perception towards a disaster not only depends on the danger it could create but also the behaviour of the communities and individuals that is governed by their culture. Within this context, this study examines the relationship between culture and disaster risk reduction (DRR). A comprehensive literature review is used for the study to evaluate culture, its components and to analyse a series of case studies related to disaster risk. It was evident from the study that in some situations, culture has become a factor for the survival of the communities from disasters where as in some situations culture has acted as a barrier for effective DRR activities. The study suggests community based DRR activities as a mechanism to integrate with culture to effectively manage disaster risk

Polyacetylenes from Sardinian Oenanthe fistulosa: A Molecular Clue to risus sardonicus

An investigation of Oenanthe fistulosa from Sardinia afforded oenanthotoxin (1a) and dihydrooenanthotoxin (1b) from the roots and the diacetylenic epoxydiol 2 from the seeds. The absolute configuration of 1a and 1b was established as R by the modified Mosher's method, and the structure of 2 by chemical correlation with (+)-(3R,8S)-falcarindiol. Oenanthotoxin (1a) and dihydrooenanthotoxin (1b) were found to potently block GABAergic responses, providing a molecular rationale for the symptoms of poisoning from water-dropwort (Oenanthe crocata) and related plants. These observations bear relevance for a series of historical and ethnopharmacological observations on the identification of the Sardonic herb and the molecular details of the facial muscular contraction caused by its ingestion (risus sardonicus)

Needle to needle robot-assisted manufacture of cell therapy products

Advanced therapeutic medicinal products (ATMPs) have emerged as novel therapies for untreatable diseases, generating the need for large volumes of high-quality, clinically-compliant GMP cells to replace costly, high-risk and limited scale manual expansion processes. We present the design of a fully automated, robot-assisted platform incorporating the use of multiliter stirred tank bioreactors for scalable production of adherent human stem cells. The design addresses a needle-to-needle closed process incorporating automated bone marrow collection, cell isolation, expansion, and collection into cryovials for patient delivery. AUTOSTEM, a modular, adaptable, fully closed system ensures no direct operator interaction with biological material; all commands are performed through a graphic interface. Seeding of source material, process monitoring, feeding, sampling, harvesting and cryopreservation are automated within the closed platform, comprising two clean room levels enabling both open and closed processes. A bioprocess based on human MSCs expanded on microcarriers was used for proof of concept. Utilizing equivalent culture parameters, the AUTOSTEM robot-assisted platform successfully performed cell expansion at the liter scale, generating results comparable to manual production, while maintaining cell quality postprocessing