7,760 research outputs found
Design of Wireless Sensor Nodes for Structural Health Monitoring applications
Enabling low-cost distributed monitoring, wireless sensor networks represents an interesting solution for the implementation of
structural health monitoring systems. This work deals with the design of wireless sensor networks for health monitoring of civil
structures, specifically focusing on node design in relation to the requirements of different structural monitoring application classes.
Design problems are analysed with specific reference to a large-scale experimental setup (the long-term structural monitoring of
the Basilica S. Maria di Collemaggio, L’Aquila, Italy). Main limitations emerged are highlighted, and adopted solution strategies
are outlined, both in the case of commercial sensing platform and of full custom solutions
Reply on the comment on the paper "Superconducting transition in Nb nanowires fabricated using focused ion beam"
In this communication we present our response to the recent comment of A.
Engel regarding our paper on FIB- fabricated Nb nanowires (see Vol. 20 (2009)
Pag. 465302). After further analysis and additional experimental evidence, we
conclude that our interpretation of the experimental results in light of QPS
theory is still valid when compared with the alternative proximity-based model
as proposed by A. Engel.Comment: 3 pages, 1 figure, accepted by Nanotechnolog
Three dimensional imaging of short pulses
We exploit a slightly noncollinear second-harmonic cross-correlation scheme
to map the 3D space-time intensity distribution of an unknown complex-shaped
ultrashort optical pulse. We show the capability of the technique to
reconstruct both the amplitude and the phase of the field through the coherence
of the nonlinear interaction down to a resolution of 10 m in space and 200
fs in time. This implies that the concept of second-harmonic holography can be
employed down to the sub-ps time scale, and used to discuss the features of the
technique in terms of the reconstructed fields.Comment: 16 pages, 6 figure
Avaliação de antibiose e não-preferência em cultivares de milho selecionados com resistência a lagarta-do-cartucho.
Dentre os fatores que contribuem para reduzir a produtividade das lavouras de milho estao as pragas, destacando-se a lagarta-do-cartucho, Spodoptera frugiperda, a qual pode reduzir a producao em ate 34%. Um dos metodos de controle de pragas, cujo custo e reduzido e nao causa efeitos indesejaveis ao ambiente, e o desenvolvimento de cultivares resistentes. Diversas fontes de resistencia foram identificadas, linhagens desenvolvidas, registradas e liberadas ao publico; entretanto, pouca informacao tem sido veiculada a respeito dos mecanismos e causas dessa resistencia. O objetivo do presente trabalho foi estudar os mecanismos de resistencia, nao-preferencia e antibiose em genotipos de milho selecionados, com resistencia a S. frugiperda. Os ensaios foram conduzidos em laboratorio e em casa de vegetacao da EMBRAPA/CNPMS. O genotipo CMS 14C apresentou antibiose influindo negativamente na biologia de S. frugiperda. 'CMS 24' e 'CMS 23' apresentaram tambem esses mecanismo, porem em menor intensidade. Foi constatada nao-preferencia alimentar das lagartas para 'Zapalote Chico' e 'BR 201'. Nao-preferencia para oviposicao foi constatada em 'CMS 14C' e 'Zapalote Chico'
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Oxidation of polycrystalline Ni studied by spectromicroscopy: Phase separation in the early stages of crystallite growth
Low-energy and photoemission electron microscopy enables the determination of facet planes of polycrystalline
surfaces and the study of their chemical composition at the sub-m scale. Using these techniques the
early oxidation stages of nickel were studied. After exposing the surface to 20 L of oxygen at 373 K a uniform
layer of chemisorbed oxygen was found on all facets. After oxygen exposure at 473–673 K, small NiO
crystallites are formed on all facets but not in the vicinity of all grain boundaries. The crystallites are separated by areas of bare Ni without significant oxygen coverage
Competing symmetries and broken bonds in superconducting vortex-antivortex molecular crystals
Hall probe microscopy has been used to image vortex-antivortex molecules induced in superconducting Pb films by the stray fields from square arrays of magnetic dots. We have directly observed spontaneous vortex-antivortex pairs and studied how they interact with added free (anti)fluxons in an applied magnetic field. We observe a variety of phenomena arising from competing symmetries which either drive added antivortices to join antivortex shells around dots or stabilize the translationally symmetric antivortex lattice between the dots. Added vortices annihilate antivortex shells, leading first to a stable “nulling state” with no free fluxons and then, at high densities, to vortex shells around the dots stabilized by the asymmetric antipinning potential. Our experimental findings are in good agreement with Ginzburg-Landau calculations
Human MicroRNAs Interacting With SARS-CoV-2 RNA Sequences: Computational Analysis and Experimental Target Validation
Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is a novel RNA virus affecting humans, causing a form of acute pulmonary respiratory disorder named COVID-19, declared a pandemic by the World Health Organization. MicroRNAs (miRNA) play an emerging and important role in the interplay between viruses and host cells. Although the impact of host miRNAs on SARS-CoV-2 infection has been predicted, experimental data are still missing. This study started by a bioinformatics prediction of cellular miRNAs potentially targeting viral RNAs; then, a number of criteria also based on experimental evidence and virus biology were applied, giving rise to eight promising binding miRNAs. Their interaction with viral sequences was experimentally validated by transfecting luciferase-based reporter plasmids carrying viral target sequences or their inverted sequences into the lung A549 cell line. Transfection of the reporter plasmids resulted in a reduction of luciferase activity for five out of the eight potential binding sites, suggesting responsiveness to endogenously expressed miRNAs. Co-transfection of the reporter plasmids along with miRNA mimics led to a further and strong reduction of luciferase activity, validating the interaction between miR-219a-2-3p, miR-30c-5p, miR-378d, miR-29a-3p, miR-15b-5p, and viral sequences. miR-15b was also able to repress plasmid-driven Spike expression. Intriguingly, the viral target sequences are fully conserved in more recent variants such as United Kingdom variant B.1.1.7 and South Africa 501Y.V2. Overall, this study provides a first experimental evidence of the interaction between specific cellular miRNAs and SARS-CoV-2 sequences, thus contributing to understanding the molecular mechanisms underlying virus infection and pathogenesis to envisage innovative therapeutic interventions and diagnostic tools
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