7,760 research outputs found

    Design of Wireless Sensor Nodes for Structural Health Monitoring applications

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    Enabling low-cost distributed monitoring, wireless sensor networks represents an interesting solution for the implementation of structural health monitoring systems. This work deals with the design of wireless sensor networks for health monitoring of civil structures, specifically focusing on node design in relation to the requirements of different structural monitoring application classes. Design problems are analysed with specific reference to a large-scale experimental setup (the long-term structural monitoring of the Basilica S. Maria di Collemaggio, L’Aquila, Italy). Main limitations emerged are highlighted, and adopted solution strategies are outlined, both in the case of commercial sensing platform and of full custom solutions

    Reply on the comment on the paper "Superconducting transition in Nb nanowires fabricated using focused ion beam"

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    In this communication we present our response to the recent comment of A. Engel regarding our paper on FIB- fabricated Nb nanowires (see Vol. 20 (2009) Pag. 465302). After further analysis and additional experimental evidence, we conclude that our interpretation of the experimental results in light of QPS theory is still valid when compared with the alternative proximity-based model as proposed by A. Engel.Comment: 3 pages, 1 figure, accepted by Nanotechnolog

    Three dimensional imaging of short pulses

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    We exploit a slightly noncollinear second-harmonic cross-correlation scheme to map the 3D space-time intensity distribution of an unknown complex-shaped ultrashort optical pulse. We show the capability of the technique to reconstruct both the amplitude and the phase of the field through the coherence of the nonlinear interaction down to a resolution of 10 μ\mum in space and 200 fs in time. This implies that the concept of second-harmonic holography can be employed down to the sub-ps time scale, and used to discuss the features of the technique in terms of the reconstructed fields.Comment: 16 pages, 6 figure

    Avaliação de antibiose e não-preferência em cultivares de milho selecionados com resistência a lagarta-do-cartucho.

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    Dentre os fatores que contribuem para reduzir a produtividade das lavouras de milho estao as pragas, destacando-se a lagarta-do-cartucho, Spodoptera frugiperda, a qual pode reduzir a producao em ate 34%. Um dos metodos de controle de pragas, cujo custo e reduzido e nao causa efeitos indesejaveis ao ambiente, e o desenvolvimento de cultivares resistentes. Diversas fontes de resistencia foram identificadas, linhagens desenvolvidas, registradas e liberadas ao publico; entretanto, pouca informacao tem sido veiculada a respeito dos mecanismos e causas dessa resistencia. O objetivo do presente trabalho foi estudar os mecanismos de resistencia, nao-preferencia e antibiose em genotipos de milho selecionados, com resistencia a S. frugiperda. Os ensaios foram conduzidos em laboratorio e em casa de vegetacao da EMBRAPA/CNPMS. O genotipo CMS 14C apresentou antibiose influindo negativamente na biologia de S. frugiperda. 'CMS 24' e 'CMS 23' apresentaram tambem esses mecanismo, porem em menor intensidade. Foi constatada nao-preferencia alimentar das lagartas para 'Zapalote Chico' e 'BR 201'. Nao-preferencia para oviposicao foi constatada em 'CMS 14C' e 'Zapalote Chico'

    Competing symmetries and broken bonds in superconducting vortex-antivortex molecular crystals

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    Hall probe microscopy has been used to image vortex-antivortex molecules induced in superconducting Pb films by the stray fields from square arrays of magnetic dots. We have directly observed spontaneous vortex-antivortex pairs and studied how they interact with added free (anti)fluxons in an applied magnetic field. We observe a variety of phenomena arising from competing symmetries which either drive added antivortices to join antivortex shells around dots or stabilize the translationally symmetric antivortex lattice between the dots. Added vortices annihilate antivortex shells, leading first to a stable “nulling state” with no free fluxons and then, at high densities, to vortex shells around the dots stabilized by the asymmetric antipinning potential. Our experimental findings are in good agreement with Ginzburg-Landau calculations

    Human MicroRNAs Interacting With SARS-CoV-2 RNA Sequences: Computational Analysis and Experimental Target Validation

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    Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is a novel RNA virus affecting humans, causing a form of acute pulmonary respiratory disorder named COVID-19, declared a pandemic by the World Health Organization. MicroRNAs (miRNA) play an emerging and important role in the interplay between viruses and host cells. Although the impact of host miRNAs on SARS-CoV-2 infection has been predicted, experimental data are still missing. This study started by a bioinformatics prediction of cellular miRNAs potentially targeting viral RNAs; then, a number of criteria also based on experimental evidence and virus biology were applied, giving rise to eight promising binding miRNAs. Their interaction with viral sequences was experimentally validated by transfecting luciferase-based reporter plasmids carrying viral target sequences or their inverted sequences into the lung A549 cell line. Transfection of the reporter plasmids resulted in a reduction of luciferase activity for five out of the eight potential binding sites, suggesting responsiveness to endogenously expressed miRNAs. Co-transfection of the reporter plasmids along with miRNA mimics led to a further and strong reduction of luciferase activity, validating the interaction between miR-219a-2-3p, miR-30c-5p, miR-378d, miR-29a-3p, miR-15b-5p, and viral sequences. miR-15b was also able to repress plasmid-driven Spike expression. Intriguingly, the viral target sequences are fully conserved in more recent variants such as United Kingdom variant B.1.1.7 and South Africa 501Y.V2. Overall, this study provides a first experimental evidence of the interaction between specific cellular miRNAs and SARS-CoV-2 sequences, thus contributing to understanding the molecular mechanisms underlying virus infection and pathogenesis to envisage innovative therapeutic interventions and diagnostic tools
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