Clinical strategies to aim an adequate safety profile for patients and effective training for surgical residents: The laparoscopic cholecystectomy model

Background Training programs for resident surgeons represent a challenge for the mentoring activity. The aim of the present study is to investigate the impact of our training program for laparoscopic cholecystectomy on patient's safety and on the modulation of the residents' exposure to clinical scenario with different grades of complexity. Material and methods This is a retrospective study based on a clinical series of laparoscopic cholecystectomy performed in a teaching hospital. Study population was grouped according to the expertise of the attending primary operator among resident surgeons. Four groups were identified: consultant (C), senior resident (SR); intermediate level resident (IR); junior resident (JR). The intraoperative and postoperative outcomes were confronted to evaluate the patient's safety profile. Results 447 patients were submitted to LC: 96 cases were operated by a C, 200 by SR, 112 by IR and 39 by JR. The mean operative time was the longest for the JR group. A statistically higher rate of conversion to open approach was registered in C and IR groups in comparison to JR and SR groups. However, in C and IR groups, patients had worse ASA score, higher BMI and more frequent past history of previous abdominal surgery, cholecystitis or pancreatitis. Overall, it was not registered any statistically significant difference among the groups in terms of length of hospital stay and prevalence of major postoperative complications. Conclusion Applying an educational model based on both graduated levels of responsibility and modulated grade of clinical complexity can guarantee an high safety profile

Efecto del manejo y del temperamento animal sobre indicadores de calidad de la carne bovina

The aim of this research was to study the effect of different handling conditions on physiological stress indicators and meat quality of beef cattle by studying animals with different temperaments. Forty animals classified by their temperament (calm and disturbed) were used. They were fed on pastures and finished with a mixed diet of corn grain and pasture. Biochemical indicators of animal stress were measured at slaughter (packed cell volume -PCV-, proteins, glucose, creatinine, alkaline phosphatase -APactivity, cortisol, insulin, glycogen). Also, ultimate pH and instrumental colour were chosen as meat quality markers. Animal temperament showed a significantly increase (p<0.05) on PCV levels and a significantly (p<0.05) decrease on muscle glycogen. Besides, levels of plasma glucose and total proteins showed significant (p<0.05) differences associated to management applied. Meat quality markers (pH and colour) did not show significant differences according to handling conditions or temperament. Mean cortisol levels at the exsanguination time were significantly higher (p<0.05) than the values obtained one week prior to slaughter, which suggests an important effect of stress associated to slaughter procedures. It would be interesting to focus attention on the assessment of acute stress at abattoir, in order to improve handling protocols, and therefore to assure meat quality in Argentinean beef production systems.El objetivo del presente trabajo fue estudiar el efecto de diferentes condiciones de manejo sobre indicadores fisiológicos de estrés y de calidad de carne en bovinos con temperamentos contrastantes. Se utilizaron cuarenta animales clasificados como calmos y excitables. Los mismos fueron alimentados inicialmente a base de pasturas y terminados con una dieta mixta de grano de maíz y pasturas. Se dosaron indicadores bioquímicos de estrés (hematocrito, proteínas plasmáticas, glucosa, creatinina, actividad fosfatasa alcalina, cortisol, insulina, contenido muscular de glucógeno). Como indicadores de calidad de carne se midieron el pH de 24 h y color instrumental. El temperamento animal demostró un incremento significativo (p<0,05) en los niveles de hematocrito y con una disminución significativa (p<0,05) del glucógeno muscular. Los niveles de glucemia y de proteínas totales mostraron modificaciones significativas (p<0,05) asociadas con el manejo. El incremento (p<0,05) en los niveles plasmáticos de cortisol durante la faena, independientemente del tratamiento o el temperamento animal, sugiere un importante efecto estresor por parte del proceso de faena. Sería interesante centrar la atención en el estudio del estrés agudo, a fin de mejorar protocolos de manejo animal, y consecuentemente, optimizar la calidad de la carne asociada en los sistemas de producción de Argentina.Fil: Pighin, D. G.. Instituto Nacional de Tecnología Agropecuaria. Centro Nacional de Investigaciones Agropecuarias. Centro de Investigación de Agroindustrias. Instituto de Tecnología de Alimentos; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad de Moron; ArgentinaFil: Davies, P.. Instituto Nacional de Tecnología Agropecuaria; ArgentinaFil: Grigioni, Gabriela Maria. Instituto Nacional de Tecnología Agropecuaria. Centro Nacional de Investigaciones Agropecuarias. Centro de Investigación de Agroindustrias. Instituto de Tecnología de Alimentos; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad de Moron; ArgentinaFil: Pazos, A. A.. Instituto Nacional de Tecnología Agropecuaria. Centro Nacional de Investigaciones Agropecuarias. Centro de Investigación de Agroindustrias. Instituto de Tecnología de Alimentos; Argentina. Universidad de Moron; ArgentinaFil: Ceconi, I.. Instituto Nacional de Tecnología Agropecuaria; ArgentinaFil: Mendez, Diego Eduardo. Instituto Nacional de Tecnología Agropecuaria; ArgentinaFil: Buffarini, M.. Instituto Nacional de Tecnología Agropecuaria; ArgentinaFil: Sancho, A.. Instituto Nacional de Tecnología Agropecuaria. Centro Nacional de Investigaciones Agropecuarias. Centro de Investigación de Agroindustrias. Instituto de Tecnología de Alimentos; Argentina. Universidad de Moron; ArgentinaFil: Gonzalez, Claudia Beatriz. Instituto Nacional de Tecnología Agropecuaria. Centro Nacional de Investigaciones Agropecuarias. Centro de Investigación de Agroindustrias. Instituto de Tecnología de Alimentos; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de San Martín. Escuela de Ciencia y Tecnología; Argentin

Video face replacement

We present a method for replacing facial performances in video. Our approach accounts for differences in identity, visual appearance, speech, and timing between source and target videos. Unlike prior work, it does not require substantial manual operation or complex acquisition hardware, only single-camera video. We use a 3D multilinear model to track the facial performance in both videos. Using the corresponding 3D geometry, we warp the source to the target face and retime the source to match the target performance. We then compute an optimal seam through the video volume that maintains temporal consistency in the final composite. We showcase the use of our method on a variety of examples and present the result of a user study that suggests our results are difficult to distinguish from real video footage.National Science Foundation (U.S.) (Grant PHY-0835713)National Science Foundation (U.S.) (Grant DMS-0739255

From reading numbers to seeing ratios: a benefit of icons for risk comprehension

Promoting a better understanding of statistical data is becoming increasingly important for improving risk comprehension and decision-making. In this regard, previous studies on Bayesian problem solving have shown that iconic representations help infer frequencies in sets and subsets. Nevertheless, the mechanisms by which icons enhance performance remain unclear. Here, we tested the hypothesis that the benefit offered by icon arrays lies in a better alignment between presented and requested relationships, which should facilitate the comprehension of the requested ratio beyond the represented quantities. To this end, we analyzed individual risk estimates based on data presented either in standard verbal presentations (percentages and natural frequency formats) or as icon arrays. Compared to the other formats, icons led to estimates that were more accurate, and importantly, promoted the use of equivalent expressions for the requested probability. Furthermore, whereas the accuracy of the estimates based on verbal formats depended on their alignment with the text, all the estimates based on icons were equally accurate. Therefore, these results support the proposal that icons enhance the comprehension of the ratio and its mapping onto the requested probability and point to relational misalignment as potential interference for text-based Bayesian reasoning. The present findings also argue against an intrinsic difficulty with understanding single-event probabilities

Tecnología NIRS para la determinación de la composición química de pechuga de pollo.

Publicado en: Revista Argentina de Producción Animal 40 (supl. 1) : (2020).La tecnología NIRS (espectroscopía en infrarrojo cercano) es una técnica instrumental que permite determinar la composición química de muestras orgánicas en forma rápida y a bajo costo. El objetivo del presente trabajo fue evaluar la capacidad de predicción de la tecnología NIRS de la composición química de pechuga de pollo de dos biotipos bajo dos sistemas de alimentación. Las calibraciones para estimar la concentración de PB, GIM, ácidos grasos saturados (AGS), ácidos grasos monoinsaturados (MUFA) y ácidos grasos poliinsaturados (PUFA) resultaron satisfactorias, con valores de R2 y RPD superiores a 0,90 y 2,70, respectivamente (Cuadro 1). Estos parámetros estadísticos fueron similares y en algunos casos mejores que los reportados por Berzaghi et al. (2005). En cuanto a la estimación del perfil de ácidos grasos de cadena larga sólo fue posible lograr calibraciones aceptables para 16-0, 18-1 n-9 y 18-2 n-6. Los estadísticos obtenidos para las ecuaciones de calibración mostraron el potencial de la tecnología NIRS para predecir la composición química de pechuga de pollo. No obstante, la utilización de esta tecnología en rutina requiere incrementar el número y la diversidad de las muestras incluídas en las calibraciones.Estación Experimental Agropecuaria Anguil "Ing. Agr. Guillermo Covas"Fil: Ortiz, Daniela Alejandra. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Anguil; Argentina.Fil: Camiletti, Fátima Karina. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Anguil; Argentina.Fil: Pordomingo, Adriana Beatriz. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Anguil; Argentina.Fil: Cunzolo, Sebastián Abel. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Investigación Tecnología de Alimentos; Argentina.Fil: Cunzolo, Sebastián Abel. Universidad de Morón. Facultad de Agronomía y Ciencias Agroalimentarias; Argentina.Fil: Pighin, Darío Gabriel. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Investigación Tecnología de Alimentos; Argentina.Fil: Pighin, Darío Gabriel. Universidad Nacional de La Pampa. Facultad de Ciencias Veterinarias; Argentina.Fil: Pighin, Darío Gabriel. Consejo Nacional de Investigaciones Científicas y Técnicas de Argentina; Argentina.Fil: Pordomingo, Anibal Javier. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Anguil; Argentina.Fil: Pordomingo, Anibal Javier. Universidad Nacional de La Pampa. Facultad de Ciencias Veterinarias; Argentina.Fil: Pordomingo, Juan N. A. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Anguil; Argentina

Regulators of floral fragrance production and their target genes in petunia are not exclusively active in the epidermal cells of petals

In which cells of the flower volatile biosynthesis takes place is unclear. In rose and snapdragon, some enzymes of the volatile phenylpropanoid/benzenoid pathway have been shown to be present in the epidermal cells of petals. It is therefore generally believed that the production of these compounds occurs in these cells. However, whether the entire pathway is active in these cells and whether it is exclusively active in these cells remains to be proven. Cell-specific transcription factors activating these genes will determine in which cells they are expressed. In petunia, the transcription factor EMISSION OF BENZENOIDS II (EOBII) activates the ODORANT1 (ODO1) promoter and the promoter of the biosynthetic gene isoeugenol synthase (IGS). The regulator ODO1 in turn activates the promoter of the shikimate gene 5-enolpyruvylshikimate-3-phosphate synthase (EPSPS). Here the identification of a new target gene of ODO1, encoding an ABC transporter localized on the plasma membrane, PhABCG1, which is co-expressed with ODO1, is described. PhABCG1 expression is up-regulated in petals overexpressing ODO1 through activation of the PhABCG1 promoter. Interestingly, the ODO1, PhABCG1, and IGS promoters were active in petunia protoplasts originating from both epidermal and mesophyll cell layers of the petal, suggesting that the volatile phenylpropanoid/benzenoid pathway in petunia is active in these different cell types. Since volatile release occurs from epidermal cells, trafficking of (volatile) compounds between cell layers must be involved, but the exact function of PhABCG1 remains to be resolved