A Comprehensive Optimization of Ultrasound\u2010Assisted Extraction for Lycopene Recovery from Tomato Waste and Encapsulation by Spray Drying

This study aimed to extract bioactive compounds from tomato waste through ultrasoundassisted extraction (UAE), using ethanol as solvent. Process optimization was carried out by a central composite design of 33 runs for response surface modelling, simultaneously analyzing the effect of temperature (T), time (t), volume (V), liquid\u2010to\u2010solid ratio (L/S), amplitude (A), the pulser duration (on), and their interaction. The best conditions found by the desirability method (T = 65 \ub0C, t = 20 min, L/S = 72 mL/g, A = 65%, on = 33 s, V = 90 mL) were experimentally verified, leading to the production of an extract with interesting properties (total carotenoids of 1408 \ub114 \u3bcglycopene equivalents/g, lycopene yield of 1536 \ub1 53 \u3bcg/g, 36.1 \ub1 0.9 \u3bcgtrolox equivalents/g as antiradical power). Due to the instability of lycopene, the extract encapsulation by spray drying was undertaken using inulin and maltodextrins as coating agents. The evaluation of wall material composition provided high product recovery (73%), a high content of encapsulated compared to superficial lycopene (15.3 \ub1 2.9 and 0.30 \ub1 0.02 \u3bcg/g), and a product with good water solubility. The novelty of this work concerned the simultaneous study of the effect and interdependences of the UAE parameters, and the use of inulin to enhance the properties of microparticles

A field study on human factor and safety performances in a downstream oil industry

afety culture and awareness by workers are pivotal tools for the implementation of systematic procedures aiming to risk mitigation in the process industry. The evaluation of human factors on safety performance can reveal unsafe attitudes and failures in training, supervision and management, whose correction greatly contribute to the enhancement of safety program. In this work, the role of human factors in an oil industry was studied by the collection of field data through a structured questionnaire filled by shift, daily and outsourced workers. A deep investigation on the variables involved in the process was carried out, firstly quantifying three conceptual key dimensions (individual, human resource management, equipment and technology) and then analyzing data by means of Response Surface Methodology (RSM), to identify the statistical significant factors and the overall level of safety awareness, behaviour and risk perception of the respondents

Accent attribution in speakers with Foreign Accent Syndrome

Purpose: The main aim of this experiment was to establish the extent to which the impression of foreignness in speakers with Foreign Accent Syndrome (FAS) is in any way comparable to the impression of foreignness in speakers with a real foreign accent. Method: Three groups of listeners attributed accents to conversational speech samples of 5 FAS speakers which were embedded amongst those of 5 speakers with a real foreign accent and 5 native speaker controls. The listener groups differed in their familiarity with foreign accented speech and speech pathology. Results: The findings indicate that listeners’ perceptual reactions to the three groups of speakers are essentially different at all levels of analysis. The native speaker controls are unequivocally considered as native speakers of Dutch while the speakers with a real foreign accent are very reliably assessed as non-native speakers. The speakers with Foreign Accent Syndrome, however, are in some sense perceived as foreign and in some sense as native by listeners, but not as foreign as speakers with a real foreign accent nor as native as real native speakers. This result may be accounted for in terms of the trigger support model of foreign accent perception. Conclusions: The findings of the experiment is consistent with the idea that the very nature of the foreign accent in different in both groups of speakers, although it cannot be fully excluded that the perceived foreignness in the two groups is one of degree

Torsional Rotordynamics of Machinery Equipment Strings

Short Cours

A predictive index of axillary nodal involvement in operable breast cancer.

We investigated the association between pathological characteristics of primary breast cancer and degree of axillary nodal involvement and obtained a predictive index of the latter from the former. In 2076 cases, 17 histological features, including primary tumour and local invasion variables, were recorded. The whole sample was randomly split in a training (75% of cases) and a test sample. Simple and multiple correspondence analysis were used to select the variables to enter in a multinomial logit model to build an index predictive of the degree of nodal involvement. The response variable was axillary nodal status coded in four classes (N0, N1-3, N4-9, N > or = 10). The predictive index was then evaluated by testing goodness-of-fit and classification accuracy. Covariates significantly associated with nodal status were tumour size (P < 0.0001), tumour type (P < 0.0001), type of border (P = 0.048), multicentricity (P = 0.003), invasion of lymphatic and blood vessels (P < 0.0001) and nipple invasion (P = 0.006). Goodness-of-fit was validated by high concordance between observed and expected number of cases in each decile of predicted probability in both training and test samples. Classification accuracy analysis showed that true node-positive cases were well recognised (84.5%), but there was no clear distinction among the classes of node-positive cases. However, 10 year survival analysis showed a superimposible prognostic behaviour between predicted and observed nodal classes. Moreover, misclassified node-negative patients (i.e. those who are predicted positive) showed an outcome closer to patients with 1-3 metastatic nodes than to node-negative ones. In conclusion, the index cannot completely substitute for axillary node information, but it is a predictor of prognosis as accurate as nodal involvement and identifies a subgroup of node-negative patients with unfavourable prognosis

API 684 - Torsional Aspects

Short Cours

Charge transport mechanisms of black diamond at cryogenic temperatures

Black diamond is an emerging material for solar applications. The femtosecond laser surface treatment of pristine transparent diamond allows the solar absorptance to be increased to values greater than 90% from semi-transparency conditions. In addition, the defects introduced by fs-laser treatment strongly increase the diamond surface electrical conductivity and a very-low activation energy is observed at room temperature. In this work, the investigation of electronic transport mechanisms of a fs-laser nanotextured diamond surface is reported. The charge transport was studied down to cryogenic temperatures, in the 30–300 K range. The samples show an activation energy of a few tens of meV in the highest temperature interval and for T &lt; 50 K, the activation energy diminishes to a few meV. Moreover, thanks to fast cycles of measurement, we noticed that the black-diamond samples also seem to show a behavior close to ferromagnetic materials, suggesting electron spin influence over the transport properties. The mentioned properties open a new perspective in designing novel diamond-based biosensors and a deep knowledge of the charge-carrier transport in black diamond becomes fundamental

Actinin BioID reveals sarcomere crosstalk with oxidative metabolism through interactions with IGF2BP2.

Actinins are strain-sensing actin cross-linkers that are ubiquitously expressed and harbor mutations in human diseases. We utilize CRISPR, pluripotent stem cells, and BioID to study actinin interactomes in human cardiomyocytes. We identify 324 actinin proximity partners, including those that are dependent on sarcomere assembly. We confirm 19 known interactors and identify a network of RNA-binding proteins, including those with RNA localization functions. In vivo and biochemical interaction studies support that IGF2BP2 localizes electron transport chain transcripts to actinin neighborhoods through interactions between its K homology (KH) domain and actinin\u27s rod domain. We combine alanine scanning mutagenesis and metabolic assays to disrupt and functionally interrogate actinin-IGF2BP2 interactions, which reveal an essential role in metabolic responses to pathological sarcomere activation using a hypertrophic cardiomyopathy model. This study expands our functional knowledge of actinin, uncovers sarcomere interaction partners, and reveals sarcomere crosstalk with IGF2BP2 for metabolic adaptation relevant to human disease