Experimental measurement of the biomass of Olea europaea L.

Olive (Olea europaea L.) is an evergreen tree extensively cultivated for its fruit in many warm parts of Southern Europe, North Africa, South Asia and Oceania. The objective of this paper was to obtain information on the total biomass productivity of the olive grove as well as on individual tree biomass productivity and the proportions of the tree components. The study was carried out in Central Italy and estimation of tree woody biomass and total biomass was made by direct measurement and through Intergovernmental Panel on Climate Change (IPCC) methodology. To determine the biomass of all the tree parts (including fruits and prunings) of the olive grove, the forestry estimation methodology named “model tree” was adopted and one representative tree was felled and uprooted; the biomass of main trunk, branches, leaves, twigs and roots was determined separately, using their volume, weight and density; also fruits and prunings of this tree were recorded. Wood density, biomass expansion factor, root/shoot ratio, carbon fraction of dry matter and carbon content of the single tree and tree component were calculated. The study showed that “model tree” methodology is suitable for olive grove; IPCC method can be successfully used with good reliability when direct measurement of biomass expansion factor and carbon fraction are obtained from a representative tree of the olive grove. The C stock evaluation methodology made in this research and the calculation of biomass expansion factor can be considered as the first scientific contribution in estimating productivity, CO2 sequestration, carbon stocks and yield of olive groves.Key words: Biomass, biomass expansion factor, Intergovernmental Panel on Climate Change (IPCC), tree volume, root/shoot ratio, Olea europaea, CO2 sequestration

Enhancement of a pentacyclic tyrosine kinase inhibitor production in Cladosporium cf. cladosporioides by Cladosporol

The binaphthyl derivative cladosporol A was supplied from 60 to 200 mg l 121 to shaken cultures of Cladosporium cf. cladosporioides. Compared to blank, fungal biomass was not affected by adding cladosporol till 100 mg l 121: it rather increased at higher ratios between 150 and 200 mg l 121. The production of the major pentacyclic metabolite 1, a cytokine production and tyrosine kinase inhibitor, was enhanced tenfold when cladosporol was supplied at the highest ratio (200 mg l 121) to shaken growing cultures of the fungus. The bioconversion of cladosporol to cladosporol D through reductive cleavage of the epoxide group was also observed. Interest in this kind of metabolites lies in their potential activity vs DNA topoisomerase I

Beads for Cell Immobilization: Comparison of Alternative Additive Manufacturing Techniques

The attachment or entrapment of microbial cells and enzymes are promising solutions for various industrial applications. When the traps are beads, they are dispersed in a fluidized bed in a vessel where a pump guarantees fresh liquid inflow and waste outflow without washing out the cells. Scientific papers report numerous types of cell entrapment, but most of their applications remain at the laboratory level. In the present research, rigid polymer beads were manufactured by two different additive manufacturing (AM) techniques in order to verify the economy, reusability, and stability of the traps, with a view toward a straightforward industrial application. The proposed solutions allowed for overcoming some of the drawbacks of traditional manufacturing solutions, such as the limited mechanical stability of gel traps, and they guaranteed the possibility of producing parts of constant quality with purposely designed exchange surfaces, which are unfeasible when using conventional processes. AM proved to be a viable manufacturing solution for beads with complex shapes of two different size ranges. A deep insight into the production and characteristics of beads manufactured by AM is provided. The paper provides biotechnologists with a manufacturing perspective, and the results can be directly applied to transit from the laboratory to the industrial scale

Acremines H\u2013N, novel prenylated polyketide metabolites produced by a strain of Acremonium byssoides

Five novel metabolites, acremines H\u2013N, have been isolated from malt extract\u2013peptone\u2013glucose agar cultures of a strain of Acremonium byssoides. Their structures and stereochemistry were elucidated using a combination of 13C and 1H homo and heteronuclear 2D NMR experiments. Acremines H\u2013N inhibited the germination of sporangia of Plasmopara viticola

Cryphonectric acid and other minor metabolites from a hypovirulent strain of Cryphonectria parasitica

Investigations carried out on secondary metabolites produced in culture by a hypovirulent strain of Cryphonectria parasitica allowed the isolation of several compounds which were characterized by NMR analysis and derivatization reactions. The most abundant metabolite was a new compound, called cryphonectric acid (1). Other metabolites were diaporthin, the only known phytotoxic compound isolated from both virulent and hypovirulent strains of C. parasitica, (+)-orthosporin, and L-p-hydroxyphenyllactic acid (HOPLA). Root growth activity of the purified compounds was evaluated both on tomato seedlings and maize subapical segments

Secondary mould metabolites of Cladosporium tenuissimum, a hyperparasite of rust fungi

Investigation of the extracts of a culture of Cladosporium tenuissimum, a known hyperparasite of several rust fungi, gave rise to the isolation of cladosporols B\u2013E (2\u20135). Their structure and stereochemistry were elucidated on the basis of 1H and 13C NMR evidence and CD measures. Cladosporols 1\u20135 were active in inhibiting the urediniospore germination of the bean rust agent Uromyces appendiculatus

The analysis of European lacquer : optimization of thermochemolysis temperature of natural resins

In order to optimize chromatographic analysis of European lacquer, thermochemolysis temperature was evaluated for the analysis of natural resins. Five main ingredients of lacquer were studied: sandarac, mastic, colophony, Manila copal and Congo copal. For each, five temperature programs were tested: four fixed temperatures (350, 480, 550, 650 degrees C) and one ultrafast thermal desorption (UFD), in which the temperature rises from 350 to 660 degrees C in 1 min. In total, the integrated signals of 27 molecules, partially characterizing the five resins, were monitored to compare the different methods. A compromise between detection of compounds released at low temperatures and compounds formed at high temperatures was searched. 650 degrees C is too high for both groups, 350 degrees C is best for the first, and 550 degrees C for the second. Fixed temperatures of 480 degrees C or UFD proved to be a consensus in order to detect most marker molecules. UFD was slightly better for the molecules released at low temperatures, while 480 degrees C showed best compounds formed at high temperatures

SECONDARY MOULD METABOLITES .42.ELEGANTHOL, A SESQUITERPENE FROM CLITOCYBE-ELEGANS

The structure and relative configuration of eleganthol, a new amorphane sesquiterpene, have been elucidated on the basis of single crystal X-ray analysis and NMR data

Isolation and structure elucidation of aza-sesquiterpenoids of protoilludane origin formed by shaken cultures of the fungus Clavicorona divaricata

Two novel sesquiterpenes of protoilludane origin, the alkaloids divaricatine C and D, have been isolated from MPGB shaken cultures of the fungus Clavicorona divaricata (Basidiomycetes). Their structures were elucidated by means of NMR studies and chemical correlations. The metabolites were weakly active against bacteria and inhibited the germination of the water cress Lepidium sativum. A possible mechanism of their formation from the protoilludane tsugicoline A (2) is suggested