2,458 research outputs found
Tumoral volume measured preoperatively by magnetic resonance imaging is related to survival in endometrial cancer.
Background. The aim of the study was to determine if the endometrial tumor volume (TV) measured by magnetic
resonance imaging (MRI-TV) is associated with survival in endometrial cancer and lymph nodes metastases (LN+).
Patients and methods. We evaluated the MRI imaging and records of 341 women with endometrial cancer and
preoperative MRI from 2008 to 2018. The MRI-TV was calculated using the ellipsoid formula measuring three perpendicular
tumor diameters. Tumor myometrial invasion was also analyzed.
Results. Higher MRI-TV was associated with age ≥ 65y, non-endometrioid tumors, grade-3, deep-myometrial invasion,
LN+ and advanced FIGO stage. There were 37 patients with LN+ (8.8%). Non-endometrioid tumors, deep-myometrial
invasion, grade-3 and MRI-TV ≥ 10 cm3 were the factors associated with LN+. Using a receiver operating characteristic
[ROC] curve, the MRI-TV cut-off for survival was 10 cm3 (area under curve [AUC] = 0.70; 95% CI: 0.61–0.73). 5 years
disease-free (DFS) and overall survival (OS) was significantly lower in MRI-TV ≥ 10 cm3 (69.3% vs. 84.5%, and 75.4% vs.
96.1%, respectively). MRI-TV was considered an independent factor of DFS (HR: 2.20, 95% CI: 1.09–4.45, p = 0.029) and
OS (HR: 3.88, 95% CI: 1.34–11.24, p = 0.012) in multivariate analysis.
Conclusions. MRI-TV was associated with LN+, and MRI-TV ≥ 10 cm3 was an independent prognostic factor of lower
DFS and OS. The MRI-TV can be auxiliary information to plan the surgery strategy and predict the adjuvant treatment
in women with endometrial cancer.post-print403 K
A general perspective of the characterization and quantification of nanoparticles: Imaging, spectroscopic, and separation techniques
This article gives an overview of the different techniques used to identify, characterize, and quantify engineered nanoparticles (ENPs). The state-of-the-art of the field is summarized, and the different characterization techniques have been grouped according to the information they can provide. In addition, some selected applications are highlighted for each technique. The classification of the techniques has been carried out according to the main physical and chemical properties of the nanoparticles such as morphology, size, polydispersity characteristics, structural information, and elemental composition. Microscopy techniques including optical, electron and X-ray microscopy, and separation techniques with and without hyphenated detection systems are discussed. For each of these groups, a brief description of the techniques, specific features, and concepts, as well as several examples, are described.Junta de Andalucía FQM-5974CEI-Biotic Granada CEI2013- MP-1
El conocimiento científico de la construcción a través de las revistas técnicas: Informes de la construcción 1948–2008
Co-expression of inducible nitric oxide synthase and arginases in different human monocyte subsets. Apoptosis regulated by endogenous NO
Human monocyte subsets, isolated from cultures of mononuclear cells, or freshly obtained from patients with multiple sclerosis, Graves' disease or pemphigus vulgaris, differed in phenotype, apoptotic features, mRNA levels of arginase II (A-II) and the inducible form of nitric oxide synthase (iNOS). Liver-type arginase I mRNA was present in all subsets. Apoptosis was followed by the expression of T cell intracellular antigen (TIA) and the simultaneous detection of DNA stainability by propidium iodine and annexin V binding. Apoptosis was practically absent both in activated CD14(++)CD33(++)DR(++)CD25(++)CD69(++)CD71(++/+) CD16(-) cells, expressing A-II mRNA and having arginase activity, but not iNOS mRNA, and in not fully mature large CD14(++)CD16(+)CD23(+)DR(++) monocytes, expressing simultaneously both mRNAs and having both enzyme activities. However, differentiated small CD14(+/++)CD16(+)CD69(+)CD25(+/-)CD71(++)CD23(+) DR(++) monocytes, expressing high levels of iNOS mRNA, exhibited apoptotic signs. Amounts of NO synthesised by monocytes co-expressing iNOS and arginase changed with the addition of arginine or an iNOS inhibitor; in that case a correlation of NO production and apoptotic features was observed. Data suggest a regulatory role for endogenous NO in apoptosis of stimulated and differentiated monocytes, and also that iNOS and A-II, when simultaneously present, could control the production of NO as a consequence of their competition for arginine
Human embryonic stem cells and derived contractile embryoid bodies are susceptible to Coxsakievirus B infection and respond to interferon Iβ treatment
We studied the susceptibility of human embryonic stem cells and derived contractile embryoid bodies from WAO9, HUES-5 and HUES-16 cell lines to Coxsackievirus B infection. After validating stem cell-like properties and cardiac phenotype, Coxsackievirus B receptors CAR and DAF, as well as type I interferon receptors were detected in all cell lines and differentiation stages studied. Real-time PCR analysis showed that CAR mRNA levels were 3.4-fold higher in undifferentiated cells, while DAF transcript levels were 2.78-fold more abundant in differentiated cultures (P5-106 plaque forming units (PFU)/ml, the highest titers were detected in undifferentiated cells. Cell viability detected by a colorimetric assay, showed inverse correlation with infectivity titers of cell culture supernatants. Treatment with 100 U of interferon Iβ significantly reduced viral replication and associated cell death during a 24-48 h observation period, as detected by reduced infectivity titers in the supernatants and increased cell viability by a colorimetric assay, respectively. We propose human embryonic stem cell and derived contractile embryoid bodies as a valid model to study cardiac Coxsackievirus B infection.Instituto de Biotecnologia y Biologia MolecularFacultad de Ciencias Exacta
Human embryonic stem cells and derived contractile embryoid bodies are susceptible to Coxsakievirus B infection and respond to interferon Iβ treatment
AbstractWe studied the susceptibility of human embryonic stem cells and derived contractile embryoid bodies from WAO9, HUES-5 and HUES-16 cell lines to Coxsackievirus B infection. After validating stem cell-like properties and cardiac phenotype, Coxsackievirus B receptors CAR and DAF, as well as type I interferon receptors were detected in all cell lines and differentiation stages studied. Real-time PCR analysis showed that CAR mRNA levels were 3.4-fold higher in undifferentiated cells, while DAF transcript levels were 2.78-fold more abundant in differentiated cultures (P<0.05). All cell lines were susceptible to Coxsackievirus serotypes B1-5 infection as shown by RT-PCR detection of viral RNA, immunofluorescence detection of viral protein and infectivity titration of cell culture supernatants resulting in cell death. Supernatants infectivity titers 24-48h post-infection ranged from 105-106 plaque forming units (PFU)/ml, the highest titers were detected in undifferentiated cells. Cell viability detected by a colorimetric assay, showed inverse correlation with infectivity titers of cell culture supernatants. Treatment with 100 U of interferon Iβ significantly reduced viral replication and associated cell death during a 24–48 h observation period, as detected by reduced infectivity titers in the supernatants and increased cell viability by a colorimetric assay, respectively. We propose human embryonic stem cell and derived contractile embryoid bodies as a valid model to study cardiac Coxsackievirus B infection
Experimental transition probabilities in NII lines
Producción CientíficaThis work reports an extensive collection of 108 transition probabilities of NII lines in the visible spectral region, all of them measured in an emission experiment. Relative intensity measurements have been made on a pulsed discharge lamp and the absolute Aki values have been obtained by using the literature available data. Electron density and temperature range in this experiment from 0.2 to and from 17000 to 29000 K respectively. The first one has been simultaneously determined from two-wavelength interferometry and from the Stark broadening of HeI 471.3 nm, the second from Boltzmann-plot of NII lines and from NII/NI intensities ratios. The results are compared with the recent available literature.
Ministerio de Educación y Formación Profesional (grant PB-98-0356)Junta de Castilla y León (grant VA23-99
Results in the parenteral nutritions electronic prescription following implementation of a computer program. Cost analysis and prescription
Introducción. Cada vez son más los hospitales que incorporan en su tecnología software informáticos
capaces de elaborar una nutrición eficaz, segura y ajustada a las necesidades de los pacientes con el fin de
minimizar errores. Sin embargo, estos programas no siempre disponen de un manejo intuitivo, lo que en
ocasiones, pueden comportar diversas dificultades que los haga estar infrautilizados y en consecuencia,
el número de prescripciones puede verse disminuido.
Objetivo: Evaluar la reducción en la prescripción de nutriciones parenterales y el impacto económico
que ha supuesto en nuestro centro, tras la implantación de un software informático de prescripción electrónica
para nutriciones parenterales.
Material y métodos. Se utilizó un software informático de Fresenius-Kabi España (v. 1.8/2011) como
base de datos, para clasificar las NP prescritas durante los últimos 24 meses, teniendo en cuenta la implantación
de la herramienta informática. Este procedimiento se usó para determinar la disminución en
la prescripción de nutriciones.
Resultados. Durante el año 2013 se prescribieron un total de 3.530 nutriciones parenterales, mientras que
en el mismo periodo de 2014, las nutriciones prescritas fueron 2.622. Esta diferencia de 908 prescripciones,
supuso un ahorro económico de 22.230,03 €.Introduction. Increasingly hospitals that incorporate in its software technology computer capable of
developing a nutrition effective, safe and adjusted to the needs of patients in order to minimize errors.
However, these programs do not always have an intuitive, sometimes, may lead to difficulties making
them to be underused and as a result, the number of prescriptions will be decreased.
Objective. To evaluate the reduction in prescription of parenteral nutritions and the economic impact
that has been in our centre, the introduction of computer software for electronic prescription for parenteral
nutritions.
Material and method. A computer software of Fresenius-Kabi Spain (v. 1.8/2011) as the database, was
used to classify the NP prescribed during the past 24 months, taking into account the implementation
of the computer tool. This procedure was used to determine the reduction in prescription of nutritions.
Results. During the year 2013 were prescribed a total of 3.530 parenteral nutritions, while in the same
period in 2014, prescribed nutritions were 2.622. This difference of 908 prescriptions meant a savings of
22.230,03 €
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