47 research outputs found

    Prerequisites for setting up management system in municipal retail trade

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    The relevance of the problem is determined by the number of complex problems that exist in present Samara municipal retail trade system, which is manifested in the lack of regulation, a glut of sales area, poorly developed infrastructure. The purpose of this article is to form a quantitative base for determining the municipal needs in the floor space. Leading method to study this problem is the method of empirical observations and mathematical modeling. The study results: the article contains the analysis of sales area availability on the example in the urban district and presents a quantitative method to determine predictive values for retail trade turnover. Practical significance: the paper is aimed to develop managerial decisions in the sphere of municipal trade regulation. Β© 2016 Suraeva et al

    Discovering Class-Specific GAN Controls for Semantic Image Synthesis

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    Prior work has extensively studied the latent space structure of GANs forunconditional image synthesis, enabling global editing of generated images bythe unsupervised discovery of interpretable latent directions. However, thediscovery of latent directions for conditional GANs for semantic imagesynthesis (SIS) has remained unexplored. In this work, we specifically focus onaddressing this gap. We propose a novel optimization method for findingspatially disentangled class-specific directions in the latent space ofpretrained SIS models. We show that the latent directions found by our methodcan effectively control the local appearance of semantic classes, e.g.,changing their internal structure, texture or color independently from eachother. Visual inspection and quantitative evaluation of the discovered GANcontrols on various datasets demonstrate that our method discovers a diverseset of unique and semantically meaningful latent directions for class-specificedits.<br

    Organizational model of the congress and exhibition cluster in the field of business travel

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    The relevance of the analyzed issue is caused by the growth of the international trade in the intensity of business travel services, including in the field of the congress and exhibition business, which necessitates the formation of the effective management model of business travel services at the destination level. The purpose of the article is to substantiate the formation algorithm of the cluster development strategy of tourism and the development of organizational functioning models of the congress and exhibition cluster in the destination. The leading approach to the study of this issue is the method of modeling, which allowed considering the practice of clusters functioning as a sequence of stages of its formation and development, taking into account the resource potential of destinations. Results: the article presents a stepwise algorithm for creating the congress and exhibition cluster in the destination and proves the fundamental organizational model for its functioning in the business tourism market; isolates and systematized criteria and indicators for assessing the contribution of participants of the tourism cluster in the destination development. The contents of this article may be useful for researchers, interested in the use of the cluster approach in the services sector, as well as to regional authorities, responsible for the development of tourism. Β© 2016 Karpova et al

    OASIS: {Only Adversarial Supervision for Semantic Image Synthesis}

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    ΠšΠΎΠ½Ρ†Π΅ΠΏΡ‚ΡƒΠ°Π»ΡŒΠ½Ρ‹Π΅ ΠΏΠΎΠ΄Ρ…ΠΎΠ΄Ρ‹ ΠΊ ΠΎΠ±Π΅ΡΠΏΠ΅Ρ‡Π΅Π½ΠΈΡŽ качСства ΠΏΠΎΠ΄Π³ΠΎΡ‚ΠΎΠ²ΠΊΠΈ ΠΊΠ°Π΄Ρ€ΠΎΠ²

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    From a conceptual position, historical development trends, current problems and prospects for ensuring the quality of personnel training were considered, taking into account the provisions of the organization management and quality management concepts. For the first time, conceptual approaches to ensuring the quality of personnel training were systematized and it was shown that they can be described by the following stages of development: in the 1900s, the concept of monitoring the quality of personnel training according to accepted standards; in the 1910s – the concept of taking into account the variability of the characteristics of the quality of training in the continuous process of learning; in the 1920s – a concept based on the large-scale use of statistical methods of quality control; in the 1930s – the concept of ensuring the reliability of knowledge and skills that guarantee the ability of trainees to keep them within the established limits at specified time intervals; in the 1940s. – a concept based on accounting in the system of personnel training for the best practical experience of previous periods of study; in the 1950s, the concept of active use in the management of training processes for quality tools; in the 1960s, with the concept of quality training, based on the TQC and CWQC quality management methodologies, taking into account the behavioral approach concept; in the 1970s, with the concept of quality training based on the TQM methodology; in the 1980s, using the concept of a management culture and the universal quality management system (UQM) as a basis; in the 1990s – the concept of quality management training, based on innovation and the concept of quality- based management (QMC); in the 2000s – the concept of quality management training, based on the concept of management by objectives; in the 2010s – the concept of quality management training, based on the active introduction of information technology; in the 2020s – the concept of quality management based on combinatorial technologies.Π‘ ΠΊΠΎΠ½Ρ†Π΅ΠΏΡ‚ΡƒΠ°Π»ΡŒΠ½Ρ‹Ρ… ΠΏΠΎΠ·ΠΈΡ†ΠΈΠΉ рассмотрСны историчСскиС Ρ‚Π΅Π½Π΄Π΅Π½Ρ†ΠΈΠΈ развития, соврСмСнныС ΠΏΡ€ΠΎΠ±Π»Π΅ΠΌΡ‹ ΠΈ пСрспСктивы обСспСчСния качСства ΠΏΠΎΠ΄Π³ΠΎΡ‚ΠΎΠ²ΠΊΠΈ ΠΊΠ°Π΄Ρ€ΠΎΠ² с ΡƒΡ‡Π΅Ρ‚ΠΎΠΌ ΠΏΠΎΠ»ΠΎΠΆΠ΅Π½ΠΈΠΉ ΠΊΠΎΠ½Ρ†Π΅ΠΏΡ†ΠΈΠΉ ΠΌΠ΅Π½Π΅Π΄ΠΆΠΌΠ΅Π½Ρ‚Π° ΠΎΡ€Π³Π°Π½ΠΈΠ·Π°Ρ†ΠΈΠΈ ΠΈ управлСния качСством. Π’ΠΏΠ΅Ρ€Π²Ρ‹Π΅ систСматизированы ΠΊΠΎΠ½Ρ†Π΅ΠΏΡ‚ΡƒΠ°Π»ΡŒΠ½Ρ‹Π΅ ΠΏΠΎΠ΄Ρ…ΠΎΠ΄Ρ‹ ΠΊ ΠΎΠ±Π΅ΡΠΏΠ΅Ρ‡Π΅Π½ΠΈΡŽ качСства ΠΏΠΎΠ΄Π³ΠΎΡ‚ΠΎΠ²ΠΊΠΈ ΠΊΠ°Π΄Ρ€ΠΎΠ² ΠΈ ΠΏΠΎΠΊΠ°Π·Π°Π½ΠΎ, Ρ‡Ρ‚ΠΎ ΠΎΠ½ΠΈ ΠΌΠΎΠ³ΡƒΡ‚ Π±Ρ‹Ρ‚ΡŒ описаны ΡΠ»Π΅Π΄ΡƒΡŽΡ‰ΠΈΠΌΠΈ этапами развития: Π² 1900‑С Π³Π³. – ΠΊΠΎΠ½Ρ†Π΅ΠΏΡ†ΠΈΠ΅ΠΉ контроля качСства ΠΏΠΎΠ΄Π³ΠΎΡ‚ΠΎΠ²ΠΊΠΈ ΠΊΠ°Π΄Ρ€ΠΎΠ² ΠΏΠΎ принятым стандартам; Π² 1910‑С Π³Π³. – ΠΊΠΎΠ½Ρ†Π΅ΠΏΡ†ΠΈΠ΅ΠΉ ΡƒΡ‡Π΅Ρ‚Π° Π²Π°Ρ€ΠΈΠ°Π±Π΅Π»ΡŒΠ½ΠΎΡΡ‚ΠΈ характСристик качСства ΠΏΠΎΠ΄Π³ΠΎΡ‚ΠΎΠ²ΠΊΠΈ ΠΊΠ°Π΄Ρ€ΠΎΠ² ΠΏΡ€ΠΈ ΠΏΠΎΡ‚ΠΎΡ‡Π½ΠΎΠΌ процСссС обучСния; Π² 1920‑С Π³Π³. – ΠΊΠΎΠ½Ρ†Π΅ΠΏΡ†ΠΈΠ΅ΠΉ, основанной Π½Π° ΠΌΠ°ΡΡˆΡ‚Π°Π±Π½ΠΎΠΌ использовании статистичСских ΠΌΠ΅Ρ‚ΠΎΠ΄ΠΎΠ² контроля качСства; Π² 1930‑С Π³Π³. – ΠΊΠΎΠ½Ρ†Π΅ΠΏΡ†ΠΈΠ΅ΠΉ обСспСчСния надСТности Π·Π½Π°Π½ΠΈΠΉ, ΡƒΠΌΠ΅Π½ΠΈΠΉ ΠΈ Π½Π°Π²Ρ‹ΠΊΠΎΠ², Π³Π°Ρ€Π°Π½Ρ‚ΠΈΡ€ΡƒΡŽΡ‰ΠΈΡ… ΡΠΏΠΎΡΠΎΠ±Π½ΠΎΡΡ‚ΡŒ ΠΎΠ±ΡƒΡ‡Π°Π΅ΠΌΡ‹Ρ… ΡΠΎΡ…Ρ€Π°Π½ΡΡ‚ΡŒ ΠΈΡ… Π² установлСнных ΠΏΡ€Π΅Π΄Π΅Π»Π°Ρ… Π² Π·Π°Π΄Π°Π½Π½Ρ‹Ρ… Π²Ρ€Π΅ΠΌΠ΅Π½Π½Ρ‹Ρ… ΠΈΠ½Ρ‚Π΅Ρ€Π²Π°Π»Π°Ρ…; Π² 1940‑С Π³Π³. – ΠΊΠΎΠ½Ρ†Π΅ΠΏΡ†ΠΈΠ΅ΠΉ, основанной Π½Π° ΡƒΡ‡Π΅Ρ‚Π΅ Π² систСмС ΠΏΠΎΠ΄Π³ΠΎΡ‚ΠΎΠ²ΠΊΠ΅ ΠΊΠ°Π΄Ρ€ΠΎΠ² Π»ΡƒΡ‡ΡˆΠ΅Π³ΠΎ практичСского ΠΎΠΏΡ‹Ρ‚Π° ΠΏΡ€Π΅Π΄Ρ‹Π΄ΡƒΡ‰ΠΈΡ… ΠΏΠ΅Ρ€ΠΈΠΎΠ΄ΠΎΠ² обучСния; Π² 1950‑С Π³Π³. – ΠΊΠΎΠ½Ρ†Π΅ΠΏΡ†ΠΈΠ΅ΠΉ Π°ΠΊΡ‚ΠΈΠ²Π½ΠΎΠ³ΠΎ использования ΠΏΡ€ΠΈ ΡƒΠΏΡ€Π°Π²Π»Π΅Π½ΠΈΠΈ процСссами ΠΏΠΎΠ΄Π³ΠΎΡ‚ΠΎΠ²ΠΊΠΈ ΠΊΠ°Π΄Ρ€ΠΎΠ² инструмСнтов качСства; Π² 1960‑С Π³Π³. – ΠΊΠΎΠ½Ρ†Π΅ΠΏΡ†ΠΈΠ΅ΠΉ качСства ΠΏΠΎΠ΄- Π³ΠΎΡ‚ΠΎΠ²ΠΊΠΈ ΠΊΠ°Π΄Ρ€ΠΎΠ², Π±Π°Π·ΠΈΡ€ΡƒΡŽΡ‰Π΅ΠΉΡΡ Π½Π° мСтодологиях управлСния качСством TQC ΠΈ CWQC с ΡƒΡ‡Π΅Ρ‚ΠΎΠΌ ΠΊΠΎΠ½Ρ†Π΅ΠΏΡ†ΠΈΠΈ повСдСнчСского ΠΏΠΎΠ΄Ρ…ΠΎΠ΄Π°; Π² 1970‑С Π³Π³. – ΠΊΠΎΠ½Ρ†Π΅ΠΏΡ†ΠΈΠ΅ΠΉ качСства ΠΏΠΎΠ΄Π³ΠΎΡ‚ΠΎΠ²ΠΊΠΈ ΠΊΠ°Π΄Ρ€ΠΎΠ², Π±Π°Π·ΠΈΡ€ΡƒΡŽΡ‰Π΅ΠΉΡΡ Π½Π° ΠΌΠ΅Ρ‚ΠΎΠ΄ΠΎΠ»ΠΎΠ³ΠΈΠΈ TQM; Π² 1980‑С Π³Π³. – использованиСм Π² качСствС основы ΠΊΠΎΠ½Ρ†Π΅ΠΏΡ†ΠΈΠΈ ΠΊΡƒΠ»ΡŒΡ‚ΡƒΡ€Ρ‹ управлСния ΠΈ систСмы ΡƒΠ½ΠΈΠ²Π΅Ρ€ΡΠ°Π»ΡŒΠ½ΠΎΠ³ΠΎ ΠΌΠ΅Π½Π΅Π΄ΠΆΠΌΠ΅Π½Ρ‚Π° качСства (UQM); Π² 1990‑С Π³Π³. – ΠΊΠΎΠ½Ρ†Π΅ΠΏΡ†ΠΈΠ΅ΠΉ управлСния качСством ΠΏΠΎΠ΄Π³ΠΎΡ‚ΠΎΠ²ΠΊΠΈ ΠΊΠ°Π΄Ρ€ΠΎΠ², основанная Π½Π° инновациях ΠΈ ΠΊΠΎΠ½Ρ†Π΅ΠΏΡ†ΠΈΠΈ ΠΌΠ΅Π½Π΅Π΄ΠΆΠΌΠ΅Π½Ρ‚Π° Π½Π° основС качСства (QMC); Π² 2000‑С Π³Π³. – ΠΊΠΎΠ½Ρ†Π΅ΠΏΡ†ΠΈΠ΅ΠΉ управлСния качСством ΠΏΠΎΠ΄Π³ΠΎΡ‚ΠΎΠ²ΠΊΠΈ ΠΊΠ°Π΄Ρ€ΠΎΠ², Π±Π°Π·ΠΈΡ€ΡƒΡŽΡ‰Π΅ΠΉΡΡ Π½Π° ΠΊΠΎΠ½Ρ†Π΅ΠΏΡ†ΠΈΠΈ управлСния ΠΏΠΎ цСлям; Π² 2010‑С Π³Π³. – ΠΊΠΎΠ½Ρ†Π΅ΠΏΡ†ΠΈΠ΅ΠΉ управлСния качСством ΠΏΠΎΠ΄Π³ΠΎΡ‚ΠΎΠ²ΠΊΠΈ ΠΊΠ°Π΄Ρ€ΠΎΠ², основанной Π½Π° Π°ΠΊΡ‚ΠΈΠ²Π½ΠΎΠΌ Π²Π½Π΅Π΄Ρ€Π΅Π½ΠΈΠΈ ΠΈΠ½Ρ„ΠΎΡ€ΠΌΠ°Ρ†ΠΈΠΎΠ½Π½Ρ‹Ρ… Ρ‚Π΅Ρ…Π½ΠΎΠ»ΠΎΠ³ΠΈΠΉ; Π² 2020‑С Π³Π³. – ΠΊΠΎΠ½Ρ†Π΅ΠΏΡ†ΠΈΠ΅ΠΉ управлСния качСством Π½Π° Π±Π°Π·Π΅ ΠΊΠΎΠΌΠ±ΠΈΠ½Π°Ρ‚ΠΎΡ€Π½Ρ‹Ρ… Ρ‚Π΅Ρ…Π½ΠΎΠ»ΠΎΠ³ΠΈΠΉ

    Input of Congress-Exhibition Services to the Economy of a Destination: Development of Assessment Methodology

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    Quantitative indicators of tourism development are presented: direct and common input of tourism to the global GDP, influence of the touristic sphere on employment and investment activity in 2015–2017. General approaches of tourism contribution evaluation to the destination’s economy are systematized. They are analyzed in the context of applicability in the meetings industry. In order to develop methodological system of evaluation of the congress-exhibition contribution to the destination development, set of indicators is proposed. It includes four basic groups with certain valuation factors: group of economical and social factors, group of connected with destination promotion to the national and world markets, group of business-factors, and group of research intensity factors of a destination’s industry sectors

    Weight method for determination of soluble Ξ²-glucans in barley grain

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    Background. Barley (Hordeum vulgare L.) is an important source of nutrients, such as starch, protein, and various dietary fibers. Ξ²-Glucans are soluble fibers found in high amounts in oat and barley grain, so they are becoming increasingly interesting due to their numerous functional and bioactive properties. The increased interest in Ξ²-glucans as a dietary supplement and a functional component of food calls for a convenient, inexpensive and affordable method for quantitative determination of these compounds.Materials and methods. An overview is given on the existing techniques for determining and isolating Ξ²-glucans in cereals: IR spectroscopy, enzymatic, colorimetric, and alkaline-enzymatic methods. Their advantages and disadvantages are shown. The disadvantages of the methods considered include high costs of reagents and equipment, duration of performance, and labor intensity.Results. This study promotes the weight method for isolation and quantitative determination of Ξ²-glucans in the grain of covered and naked barley. It is based on the modified alkaline method adapted to barley; we developed it earlier for oat grain. The amount of Ξ²-glucans in the grain of the studied barley accessions ranged from 4.12Β±0.23% to 5.34 Β± 0.31% for naked cultivars, and from 3.57 Β± 0.18% to 4.29 Β± 0.32% for covered ones.Conclusion. Based on the conducted research, optimal conditions for the isolation and quantitative determination of Ξ²-glucans from barley grain were selected: centrifugation modes, temperature and extraction ratio, concentration of compounds, precipitation and drying procedures. The main advantage of the described method is its accessibility and practical applicability when conducting mass analysis, including studying a collection of cereal crops

    ВСроятныС Ρ‚Ρ€Π΅Π½Π΄Ρ‹ трансформации содСрТания ΠΏΡ€ΠΎΠ³Ρ€Π°ΠΌΠΌΡ‹ Π±Π°ΠΊΠ°Π»Π°Π²Ρ€ΠΈΠ°Ρ‚Π° ΠΏΠΎ Π½Π°ΠΏΡ€Π°Π²Π»Π΅Π½ΠΈΡŽ ΠΏΠΎΠ΄Π³ΠΎΡ‚ΠΎΠ²ΠΊΠΈ Β«ΠΌΠ΅Π½Π΅Π΄ΠΆΠΌΠ΅Π½Ρ‚Β» Π² условиях ΠΏΠΎΡΡ‚ΠΈΠ½Π΄ΡƒΡΡ‚Ρ€ΠΈΠ°Π»ΡŒΠ½ΠΎΠΉ экономики

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    The paper considers processes of the managerial activity dynamics in the context of changing the economy technological patterns accompanied by the global financial crisis, and aggravated by the coronavirus pandemic, which led to localization, slowdown and even partial paralysis of many processes of socioeconomic development. Through the prism of ongoing the society socioeconomic changes, probable trends are described which transform the undergraduate curriculum content of the training direction Β«ManagementΒ» in the higher education system in modern conditions of the post-industrial economy development. As a description results of the process evolution of developing the practical applied directions of management synchronized with the concept evolution of developing management as a science formed within the framework of economic activity business cycles by K. Zhuglyar, it is shown that the next decade (2020-2030) the greatest development in the management practice (due to the socioeconomic development characteristics in the reviewed period) will receive such areas as communication management and coordination management.РассмотрСны процСссы Π΄ΠΈΠ½Π°ΠΌΠΈΠΊΠΈ управлСнчСской Π΄Π΅ΡΡ‚Π΅Π»ΡŒΠ½ΠΎΡΡ‚ΠΈ Π² условиях происходящСй смСны тСхнологичСских ΡƒΠΊΠ»Π°Π΄ΠΎΠ² экономики, ΡΠΎΠΏΡ€ΠΎΠ²ΠΎΠΆΠ΄Π°ΡŽΡ‰Π΅ΠΉΡΡ ΠΌΠΈΡ€ΠΎΠ²Ρ‹ΠΌ финансовым кризисом ΠΈ отягощСнной ΠΏΠ°Π½Π΄Π΅ΠΌΠΈΠ΅ΠΉ коронавируса, ΠΏΡ€ΠΈΠ²Π΅Π΄ΡˆΠ΅ΠΉ ΠΊ Π»ΠΎΠΊΠ°Π»ΠΈΠ·Π°Ρ†ΠΈΠΈ, замСдлСнию ΠΈ Π΄Π°ΠΆΠ΅ частичной ΠΏΠ°Ρ€Π°Π»ΠΈΠ·Π°Ρ†ΠΈΠΈ ΠΌΠ½ΠΎΠ³ΠΈΡ… процСссов ΡΠΎΡ†ΠΈΠ°Π»ΡŒΠ½ΠΎ-экономичСского развития. Бквозь ΠΏΡ€ΠΈΠ·ΠΌΡƒ происходящих ΡΠΎΡ†ΠΈΠ°Π»ΡŒΠ½ΠΎ-экономичСских ΠΈΠ·ΠΌΠ΅Π½Π΅Π½ΠΈΠΉ Π² общСствС описаны вСроятныС Ρ‚Ρ€Π΅Π½Π΄Ρ‹ трансформации содСрТания ΡƒΡ‡Π΅Π±Π½Ρ‹Ρ… ΠΏΠ»Π°Π½ΠΎΠ² ΠΏΡ€ΠΎΠ³Ρ€Π°ΠΌΠΌΡ‹ Π±Π°ΠΊΠ°Π»Π°Π²Ρ€ΠΈΠ°Ρ‚Π° ΠΏΠΎ Π½Π°ΠΏΡ€Π°Π²Π»Π΅Π½ΠΈΡŽ ΠΏΠΎΠ΄Π³ΠΎΡ‚ΠΎΠ²ΠΊΠΈ Β«ΠΌΠ΅Π½Π΅Π΄ΠΆΠΌΠ΅Π½Ρ‚Β» Π² систСмС Π²Ρ‹ΡΡˆΠ΅Π³ΠΎ образования Π² соврСмСнных условиях развития ΠΏΠΎΡΡ‚ΠΈΠ½Π΄ΡƒΡΡ‚Ρ€ΠΈΠ°Π»ΡŒΠ½ΠΎΠΉ экономики. Π’ Ρ€Π΅Π·ΡƒΠ»ΡŒΡ‚Π°Ρ‚Π΅ описания ΡΠ²ΠΎΠ»ΡŽΡ†ΠΈΠΈ процСссов развития практичСских ΠΏΡ€ΠΈΠΊΠ»Π°Π΄Π½Ρ‹Ρ… Π½Π°ΠΏΡ€Π°Π²Π»Π΅Π½ΠΈΠΉ ΠΌΠ΅Π½Π΅Π΄ΠΆΠΌΠ΅Π½Ρ‚Π°, синхронизированных с ΡΠ²ΠΎΠ»ΡŽΡ†ΠΈΠ΅ΠΉ ΠΊΠΎΠ½Ρ†Π΅ΠΏΡ†ΠΈΠΉ развития ΠΌΠ΅Π½Π΅Π΄ΠΆΠΌΠ΅Π½Ρ‚Π° ΠΊΠ°ΠΊ Π½Π°ΡƒΠΊΠΈ, Ρ„ΠΎΡ€ΠΌΠΈΡ€ΡƒΡŽΡ‰ΠΈΡ…ΡΡ Π² Ρ€Π°ΠΌΠΊΠ°Ρ… Π΄Π΅Π»ΠΎΠ²Ρ‹Ρ… Ρ†ΠΈΠΊΠ»ΠΎΠ² экономичСской активности К. Жугляра, ΠΏΠΎΠΊΠ°Π·Π°Π½ΠΎ, Ρ‡Ρ‚ΠΎ Π² блиТайшСС дСсятилСтиС (2020–2030‑С Π³Π³.) наибольшСС Ρ€Π°Π·Π²ΠΈΡ‚ΠΈΠ΅ Π² ΠΏΡ€Π°ΠΊΡ‚ΠΈΠΊΠ΅ управлСния (Π² силу особСнностСй ΡΠΎΡ†ΠΈΠ°Π»ΡŒΠ½ΠΎ-экономичСского развития Π² рассматриваСмый ΠΏΠ΅Ρ€ΠΈΠΎΠ΄) ΠΏΠΎΠ»ΡƒΡ‡Π°Ρ‚ Ρ‚Π°ΠΊΠΈΠ΅ направлСния, ΠΊΠ°ΠΊ ΠΊΠΎΠΌΠΌΡƒΠ½ΠΈΠΊΠ°Ρ†ΠΈΠΎΠ½Π½Ρ‹ΠΉ ΠΌΠ΅Π½Π΅Π΄ΠΆΠΌΠ΅Π½Ρ‚ ΠΈ ΠΊΠΎΠΎΡ€Π΄ΠΈΠ½Π°Ρ†ΠΈΠΎΠ½Π½Ρ‹ΠΉ ΠΌΠ΅Π½Π΅Π΄ΠΆΠΌΠ΅Π½Ρ‚

    Assessment of Residual Virulence of Francisella tularensis 15 NIIEG Vaccine Strain Based on Long-term Observations

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    Objective of the study is to assess and analyze the long-term data on annual control of residual virulence of Francisella tularensis 15 NIIEG vaccine strain for clarifying the value of the parameter and amending the regulatory documentation. Materials and methods. Utilized were 8 vials containing lyophilized cultures of vaccine strain F. tularensis 15 NIIEG dated 1953, 1966, 1969, 1987, 1990, 2003, 2012, and 2013, manufactured at different industrial sites. To gather additional information on residual virulence of F. tularensis 15 NIIEG strain, evaluation of quality control files of 76 lyophilized cultures in vials was performed, out of which 48 strains manufactured at the premises of Odessa Bacterial Products Enterprise in 1980, 1987, and 1990, and 28 – at Joint Stock Company Scientific Production Association on Medical Immunobiological Preparations β€œMicrogen”, Omsk Bacterial Products Enterprise, in 2003–2013. Results and discussion. Assessment of the parameter has revealed that out of 8 tested cultures of F. tularensis 15 NIIEG strain of various date of lyophilization 7 cultures have virulence rate ranging within 1Β·102 – 2.5Β·102 mc, LD50 of the strain dated 1966 is 7.3Β·105 mc (the standard range 1Β·102 – 2Β·106 mc). Obtained in the course of analysis of quality control files on F. tularensis 15 NIIEG strain, stored in lyophilized form at (19Β±1) Β°C, data demonstrate that residual virulence stays within the specified limits. Amendments regarding the value of β€œResidual virulence” parameter have been introduced into the regulatory documentation, the level ranging within 1Β·102 – 5Β·103 mc

    Comparative analysis of the chemical composition and size of starch granules in grain between diploid and tetraploid sweetcorn cultivars

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    Background. Improving the taste and nutritional value of sweetcorn using genetic selection techniques is an important trend. The tetraploid sugary maize cultivar β€˜Baksanskaya sakharnaya’ (k-23426) has a number of advantages in terms of taste and marketability over traditional diploid varieties.The purpose of the research was to identify the distinctive biochemical characteristics that affect the taste of this cultivar in comparison with the diploid reference (cv. β€˜Rannyaya Lakomka’, с-1775).Materials and methods. The differences in phenotypic traits between diploid (2n) and tetraploid (4n) maize plants were assessed. Infrared spectroscopy was used to study the difference in the biochemical composition of kernels in terms of protein, starch and oil content. Accumulation of metabolites in a dry kernel was studied using gas–liquid chromatography with mass spectrometry.Results. Comparison of the phenotypic traits in 2n and 4n sweetcorn plants showed that 4n had a better ear structure, protein and oil content. Analysis of metabolites showed that quantitative indicators of chemical components changed in kernels of the 4n sweetcorn without changing the qualitative composition. The chemical composition of the metabolites in 4n sweetcorn grain was characterized by an increase in the synthesis of most organic acids and protein amino acids, phosphoric acid, fatty acids, some monosaccharides, phenolic compounds, and phytosterols. A decrease in the content of most polyhydric alcohols, disaccharides, trisaccharides, some monosaccharides, and phenolic compounds was observed. An inhibitory effect of a su2 gene dose on the size of starch granules was found.Β Conclusion. The studies showed the advantage of the 4n sweetcorn over the 2n one due to its improved ear structure and biochemical composition, which positively affected the taste qualities
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