Organic Compounds and Genotoxicity in Drinking Water

Until recently only lipophilic compounds were analysed in any research on the occurrence of mutanogenic and carcinogenic compounds in water. They were isolated using XAD-resins. They contain approximately half of the total organic material present in water. A clear mutanogenic effect was demonstrated for these compounds using Amestest. The hydrophilic fraction of the organic material was difficult to investigate because of problems with isolation and analysis caused by its high solubility. The high solubility means in practice that the hydrophilic compounds are mobile in conventional treatment systems and in soil and can easily penetrate into drinking water. A method was developed to isolate hydrophilic compounds using a combination of ion exchange and a clean-up with a XAD-resin. The isolated compounds were examined with the Amestest for the presence of mutanogenic compounds. For some tested water types a mutanogenic effect was found in the lipophilic material. Due to oxidation with ozone, mutanogenic compounds can be formed from (non-mutanogenic) industrial pollutions. No mutanogenic activity was found in the hydrophilic organic material of all examined water types, even after ozone oxidation or chlorination. It was hypothesized that hydrophilic compounds can not reach the DNA of the cell, thus they do not play any (geno) toxicological role

Instability of LBV-stars against radial oscillations

In this study we consider the nonlinear radial oscillations exciting in LBV--stars with effective temperatures 1.5e4 K <= Teff <= 3e4 K, bolometric luminosities 1.2e6 L_odot <= L <= 1.9e6 L_odot and masses 35.7 M_odot <= M <= 49.1 M_odot. Hydrodynamic computations were carried out with initial conditions obtained from evolutionary sequences of population I stars (X=0.7, Z=0.02) with initial masses from 70M_odot to 90 M_odot. All hydrodynamical models show instability against radial oscillations with amplitude growth time comparable with dynamical time scale of the star. Radial oscillations exist in the form of nonlinear running waves propagating from the boundary of the compact core to the upper boundary of the hydrodynamical model. The velocity amplitude of outer layers is of several hundreds of km/s while the bolometric light amplitude does not exceed 0.2 mag. Stellar oscillations are not driven by the kappa-mechanism and are due to the instability of the gas with adiabatic exponent close to the critical value Gamma_1 = 4/3 due to the large contribution of radiation in the total pressure. The range of the light variation periods (6 day <= P <= 31 day) of hydrodynamical models agrees with periods of microvariability observed in LBV--stars.Comment: 14 pages, 5 figures, submitted to Astronomy Letter

Galactic Twins of the Ring Nebula Around SN1987A and a Possible LBV-like Phase for Sk-69 202

Some core-collapse supernovae show clear signs of interaction with dense circumstellar material that often appears to be non-spherical. Circumstellar nebulae around supernova progenitors provide clues to the origin of that asymmetry in immediate pre-supernova evolution. Here I discuss outstanding questions about the formation of the ring nebula around SN1987A and some implications of similar ring nebulae around Galactic B supergiants. Several clues hint that SN1987A's nebula may have been ejected in an LBV-like event, rather than through interacting winds in a transition from a red supergiant to a blue supergiant.Comment: 2 pages, to appear in procedings of "Massive stars: fundamental parameters and circumstellar interactions", conference in honor of Virpi Niemela's 70th birthda

On the nature of the prototype LBV AG Carinae I. Fundamental parameters during visual minimum phases and changes in the bolometric luminosity during the S-Dor cycle

We present a detailed spectroscopic analysis of the luminous blue variable AG Carinae during the last two visual minimum phases of its S-Dor cycle (1985-1990 and 2000-2003). The analysis reveals an overabundance of He, N, and Na, and a depletion of H, C, and O, on the surface of AG Car, indicating the presence of CNO-processed material. Furthermore, the ratio N/O is higher on the stellar surface than in the nebula. We found that the minimum phases of AG Car are not equal to each other, since we derived a noticeable difference between the maximum effective temperature achieved during 1985-1990 (22,800 K) and 2000-2001 (17,000 K). While the wind terminal velocity was 300 km/s in 1985-1990, it was as low as 105 km/s in 2001. The mass-loss rate, however, was lower from 1985-1990 (1.5 x 10^(-5) Msun/yr) than from 2000-2001 (3.7 x 10^(-5) Msun/yr). We found that the wind of AG Car is significantly clumped (f=0.10 - 0.25) and that clumps must be formed deep in the wind. We derived a bolometric luminosity of 1.5 x 10^6 Lsun during both minimum phases which, contrary to the common assumption, decreases to 1.0 x 10^6 Lsun as the star moves towards maximum flux in the V band. Assuming that the decrease in the bolometric luminosity of AG Car is due to the energy used to expand the outer layers of the star (Lamers 1995), we found that the expanding layers contain roughly 0.6 - 2 Msun. Such an amount of mass is an order of magnitude lower than the nebular mass around AG Car, but is comparable to the nebular mass found around lower-luminosity LBVs and to that of the Little Homunculus of Eta Car. If such a large amount of mass is indeed involved in the S Dor-type variability, we speculate that such instability could be a failed Giant Eruption, with several solar masses never becoming unbound from the star.(abridged)Comment: 22 pages, 13 figures, ApJ in press. A high-resolution PDF version is also available at http://www.mpifr-bonn.mpg.de/staff/jgroh/agcar.htm

Hyponatraemia in imported malaria is common and associated with disease severity

<p>Abstract</p> <p>Background</p> <p>Hyponatraemia (serum sodium < 135 mmol/L) has long been recognized as a complication of malaria. However, few studies have been done in non-immune adult populations. It has not been investigated previously how hyponatraemia is distributed among the various <it>Plasmodium </it>species, and its association with malaria severity is unknown.</p> <p>The aim of this retrospective cohort study was to determine the prevalence of hyponatraemia and its association with malaria severity in a large cohort of patients with imported malaria caused by various <it>Plasmodium </it>species.</p> <p>Methods</p> <p>All patients that were diagnosed with malaria in the Harbour Hospital and Institute for Tropical Diseases in Rotterdam in the period 1999-2009 and who had available serum sodium on admission were included. Severe malaria was defined according to the modified WHO criteria. Prevalence of hyponatraemia and its association with malaria severity were investigated by univariate comparison, ROC analysis and multivariate logistic regression analysis.</p> <p>Results</p> <p>A total of 446 patients with malaria (severe falciparum malaria n = 35, non-severe falciparum malaria n = 280, non-falciparum malaria n = 131) was included. Hyponatraemia was present in 207 patients (46%). Prevalence and severity of hyponatraemia were greatest in severe falciparum malaria (77%, median serum sodium 129 mmol/L), followed by non-severe falciparum malaria (48%, median serum sodium 131 mmol/L), and non-falciparum malaria (34%, median serum sodium 132 mmol/L). Admission serum sodium < 133 mmol/L had a sensitivity of 0.69 and a specificity of 0.76 for predicting severe malaria. Multivariate logistic regression showed that serum sodium < 131 mmol/L was independently associated with severe falciparum malaria (odds ratio 10.4, 95% confidence interval 3.1-34.9). In patients with hyponatraemia, hypovolaemia did not appear to play a significant role in the development of hyponatraemia when prerenal azotaemia and haematocrit were considered as surrogate markers for hypovolaemia.</p> <p>Conclusions</p> <p>Hyponatraemia is common in imported malaria and is associated with severe falciparum malaria. From a clinical point of view, the predictive power of hyponatraemia for severe malaria is limited. The precise pathophysiological mechanisms of hyponatraemia in malaria require further study.</p

The highly polarized open cluster Trumpler 27

We have carried out multicolor linear polarimetry (UBVRI) of the brightest stars in the area of the open cluster Trumpler 27. Our data show a high level of polarization in the stellar light with a considerable dispersion, from $P = 4$ to $P = 9.5$. The polarization vectors of the cluster members appear to be aligned. Foreground polarization was estimated from the data of some non-member objects, for which two different components were resolved: the first one associated with a dust cloud close to the Sun producing $P_{\lambda max}=1.3$ and $\theta=146$ degrees, and a second component, the main source of polarization for the cluster members, originated in another dust cloud, which polarizes the light in the direction of $\theta= 29.5$ degrees. From a detailed analysis, we found that the two components have associated values $E_{B-V} < 0.45$ for the first one, and $E_{B-V} > 0.75$ for the other. Due the difference in the orientation of both polarization vectors, almost 90 degrees (180 degrees at the Stokes representation), the first cloud ($\theta \sim 146$ degrees) depolarize the light strongly polarized by the second one ($\theta \sim 29.5$ degrees).Comment: 12 Pages, 6 Figures, 2 tables (9 Pages), accepted for publication in A

Neopterin and procalcitonin are suitable biomarkers for exclusion of severe Plasmodium falciparum disease at the initial clinical assessment of travellers with imported malaria

Background. Most clinicians in developed, non-malaria endemic countries have limited or no experience in making clinical assessments of malaria disease severity and subsequent decisions regarding the need for parenteral therapy or high-level monitoring in febrile patients with imported malaria. In the present study, the diagnostic accuracy of plasma soluble Triggering Receptor Expressed on Myeloid cells 1 (TREM-1), neopterin and procalcitonin levels as biomarkers for severe Plasmodium falciparum disease was evaluated in 104 travellers with imported malaria (26 patients with non-P. falciparum malaria, 64 patients with uncomplicated P. falciparum malaria and 14 patients with severe P. falciparum malaria). Methods. TREM-1, neopterin and procalcitonin were determined in serum using commercially available ELISA or EIA tests. The diagnostic performance of these biomarkers for severe disease was compared with plasma lactate, a well-validated parameter for disease severity in patients with malaria, as reference. Severe malaria was defined according to the modified WHO criteria. Results. No significant differences in TREM-1 levels were detected between the different patient groups. Patients with severe P. falciparum malaria had significantly higher neopterin and procalcitonin levels on admission when compared to patients with uncomplicated P. falciparum malaria or non-P. falciparum malaria. Receiver Operating Characteristic (ROC) curve analysis showed that neopterin had the highest Area-Under-the-ROC curve (AUROC 0.85) compared with plasma lactate (AUROC 0.80) and procalcitonin (AUROC 0.78). At a cut-off point of 10.0 ng/ml, neopterin had a positive and negative predictive value of 0.38 and 0.98 whereas procalcitonin, at a cut-off point of 0.9 ng/ml, had a positive and negative predictive value of 0.30 and 1.00. Conclusion. Although the diagnostic value of neopterin and procalcitonin is limited, the high negative predictive value of both neopterin and procalcitonin may be helpful for a rapid exclusion of severe malaria disease on admission. This may be a valuable tool for physicians only occasionally dealing with ill-returned travellers from malaria-endemic regions and who need to decide on subsequent oral anti-malarial treatment or timely referral to a specialized centre for high-level monitoring and intensified parenteral treatment

Procalcitonin as a Biomarker for a Bacterial Infection on Hospital Admission: A Critical Appraisal in a Cohort of Travellers with Fever after a Stay in (Sub)tropics

Fever in a returned traveller may be the manifestation of a self-limiting, trivial infection but it can also presage an infection that can be rapidly progressive and lethal. We studied the diagnostic accuracy of procalcitonin (PCT) as a biomarker for a bacterial cause of fever in a cohort of 157 consecutive travellers with fever after a stay in the (sub)tropics. Elevated procalcitonin levels were observed not only in about 50% of travellers with proven bacterial infection, but also in a significant proportion of travellers with a likely infection. Using a cutoff point of 0.5 ng/mL, procalcitonin had a sensitivity of 0.52 and a specificity of 0.76 for a bacterial cause of fever on admission. Interestingly, only 1 out of 16 patients with a proven viral infection had a marginally elevated PCT concentration on admission, suggesting that an increased PCT level likely excludes a viral infection as the cause of fever. However, the diagnostic accuracy of this semiquantitative procalcitonin test for a bacterial cause of fever on admission is too poor to advocate its use in the initial clinical evaluation of fever in a setting of ill-returned travellers