Anti-tumor properties of blackseed (Nigella sativa L.) extracts

Abstract The objective of the present study was to evaluate the in vitro and in vivo anti-cancer effect of Nigella sativa L. seed extracts. The essential oil (IC 50 = 0.6%, v/v) and ethyl acetate (IC 50 = 0.75%) extracts were more cytotoxic against the P815 cell line than the butanol extract (IC 50 = 2%). Similar results were obtained with the Vero cell line. Although all extracts had a comparable cytotoxic effect against the ICO1 cell line, with IC 50 values ranging from 0.2 to 0.26% (v/v), tests on the BSR cell line revealed a high cytotoxic effect of the ethyl acetate extract (IC 50 = 0.2%) compared to the essential oil (IC 50 = 1.2%). These data show that the cytotoxicity of each extract depends on the tumor cell type. In vivo, using the DBA2/P815 (H 2 d ) mouse model, our results clearly showed that the injection of the essential oil into the tumor site significantly inhibited solid tumor development. Indeed, on the 30th day of treatment, the tumor volume of the control animals was 2.5 ± 0.6 cm 3 , whereas the tumor volumes of the essential oil-treated animals were 0.22 ± 0.1 and 0.16 ± 0.1 cm 3 when the animals were injected with 30 µL (28.5 mg)/mouse and 50 µL (47.5 mg)/mouse per 48 h (six times), respectively. Interestingly, the administration of the essential oil into the tumor site inhibited the incidence of liver metastasis development and improved mouse survival. Correspondence A. Zya

Anti-tumor properties of black seed (Nigella Sativa)extract

Abstract The objective of the present study was to evaluate the in vitro and in vivo anti-cancer effect of Nigella sativa L. seed extracts. The essential oil (IC 50 = 0.6%, v/v) and ethyl acetate (IC 50 = 0.75%) extracts were more cytotoxic against the P815 cell line than the butanol extract (IC 50 = 2%). Similar results were obtained with the Vero cell line. Although all extracts had a comparable cytotoxic effect against the ICO1 cell line, with IC 50 values ranging from 0.2 to 0.26% (v/v), tests on the BSR cell line revealed a high cytotoxic effect of the ethyl acetate extract (IC 50 = 0.2%) compared to the essential oil (IC 50 = 1.2%). These data show that the cytotoxicity of each extract depends on the tumor cell type. In vivo, using the DBA2/P815 (H 2 d ) mouse model, our results clearly showed that the injection of the essential oil into the tumor site significantly inhibited solid tumor development. Indeed, on the 30th day of treatment, the tumor volume of the control animals was 2.5 ± 0.6 cm 3 , whereas the tumor volumes of the essential oil-treated animals were 0.22 ± 0.1 and 0.16 ± 0.1 cm 3 when the animals were injected with 30 µL (28.5 mg)/mouse and 50 µL (47.5 mg)/mouse per 48 h (six times), respectively. Interestingly, the administration of the essential oil into the tumor site inhibited the incidence of liver metastasis development and improved mouse survival

Effets comparés et interactifs des pesticides et des facteurs physiques sur la minéralisation de divers substrats carbonés dans le sol

*INRA BP 86510 21065 Dijon cedex (FRA) Diffusion du document : INRA BP 86510 21065 Dijon cedex (FRA) Diplôme : Dr. d'Universit

Comparison of three methods to test the side effects of pesticides on soil microbial biomass

International audienc

CARACTERISATIONS PHYSICO-CHIMIQUE ET MICROBIOLOGIQUE DE LA VIANDE HACHEE DU DROMADAIRE ISSUE DES REGIONS DE CASABLANCA, RABAT ET SALE

The camel is an animal often recognized in the Saharan areas used for work, leather, milk and meat products. The quality of the camel meat depends on its conformity to the Moroccan legislation and certain constraints. In our study 25 samples of camel ground meats were taken from various areas, Rabat (Youssofia), Salé (Souk lekhmis), Témara (Souk Sebt) and Casablanca (Souk Ejamiaa). The established characterizations were the physicochemical (pH, redox potential and the matter dries) and microbiological (total mesophil aerobic flora, total and faecal coliforms, Salmonella, Staphylococcus aureus, Listeria monocytogenes, yeasts andlactic bacteria). The results obtained were confronted with the same analyses carried out on the beef ground meat in Rabat-Sale area and with the national and international standards.The comparison between the dromedary meat and the beef meat have showed that the redox potential average twice value in the dromedary meat. The matter dries average of the dromedary is less low than that of the beef. The total count bacteria and total coliforms of thedromedary ground meat were one Log ufc/g low than the beef ground meat.Le dromadaire est un animal reconnu souvent dans les régions sahariennes, pour être utilisés pour la production de travail, de cuir, de lait et de la viande. La qualité de la viande de dromadaire dépend de sa conformité à la législation marocaine et de certaines contraintes liées à la rareté de ce produit. Dans notre étude 25 échantillons de viandes hachées de dromadaire ont été prélevés à partir de différents points de ventes des régions de Rabat, Salé (Souk lekhmis), Témara (Souk Sebt) et Casablanca (Souk Ejamiaa). Des caractérisations physico-chimiques (pH, le potentiel d’oxydo-reduction et la matière sèche) et microbiologique (flore mésophile aérobie totale, coliformes totaux et coliformes fécaux, Salmonella, Staphylococcus aureus, Clostridium perfringens, Listeria monocytogenes, levures et bactéries lactiques). Les résultats obtenus ont été comparés aux résultats des mêmes analyses réalisées sur la viande hachée de boeuf des régions de Rabat et salé et aux critères nationaux et internationaux. Les résultats montrent que les valeurs du potentiel d’oxydo-reduction sur des échantillons de viande hachée de dromadaire sont beaucoup plus importantes que celles des échantillons de viande hachés de boeuf, Ceci rend la viande du dromadaire résistante à la multiplication microbienne. Alors que la matière sèche des échantillons de dromadaire est moins élevée que celle des échantillons de boeuf. De point de vue microbiologique les charges en flore mésophile aérobie totale et en coliformes totaux énumérés sur des échantillons de viande de dromadaire sont inférieures de un1 Log ufc/g à celles trouvées sur les échantillons de boeuf

Cytotoxic effect of essential oil of thyme (Thymus broussonettii) on the IGR-OV1 tumor cells resistant to chemotherapy

The anti-tumor effect of the Moroccan endemic thyme (Thymus broussonettii) essential oil (EOT) was investigated in vitro using the human ovarian adenocarcinoma IGR-OV1 parental cell line OV1/P and its chemoresistant counterparts OV1/adriamycin (OV1/ADR), OV1/vincristine (OV1/VCR), and OV1/cisplatin (OV1/CDDP). All of these cell lines elicited various degrees of sensitivity to the cytotoxic effect of EOT. The IC50 values (mean ± SEM, v/v) were 0.40 ± 0.02, 0.39 ± 0.02, 0.94 ± 0.05, and 0.65 ± 0.03% for OV1/P, OV1/ADR, OV1/VCR, and OV1/CDDP, respectively. Using the DBA-2/P815 (H2d) mouse model, tumors were developed by subcutaneous grafting of tumor fragments of similar size obtained from P815 (murin mastocytoma cell line) injected in donor mouse. Interestingly, intra-tumoral injection of EOT significantly reduced solid tumor development. Indeed, by the 30th day of repeated EOT treatment, the tumor volumes of the animals were 2.00 ± 0.27, 1.35 ± 0.20, and 0.85 ± 0.18 cm³ after injection with 10, 30, or 50 µL per 72 h (six times), respectively, as opposed to 3.88 ± 0.50 cm³ for the control animals. This tumoricidal effect was associated with a marked decrease of mouse mortality. In fact, in these groups of mice, the recorded mortality by the 30th day of treatment was 30 ± 4, 18 ± 4, and 8 ± 3%, respectively, while the control animals showed 75 ± 10% of mortality. These data indicate that the EOT which contains carvacrol as the major component has an important in vitro cytotoxic activity against tumor cells resistant to chemotherapy as well as a significant antitumor effect in mice. However, our data do not distinguish between carvacrol and the other components of EOT as the active factor