Adherence to and Invasion of Human Intestinal Cells by Arcobacter Species and Their Virulence Genotypes

The genus Arcobacter is composed of 17 species which have been isolated from various sources. Of particular interest are A. butzleri, A. cryaerophilus, and A. skirrowii, as these have been associated with human cases of diarrhea, the probable transmissionrroutes being through the ingestion of contaminated drinking water and food. To date, only limited studies of virulence traits in this genus have been undertaken. The present study used 60 Arcobacter strains isolated from different sources, representing 16 of the 17 species of the genus, to investigate their ability to adhere to and invade the human intestinal cell line Caco-2. In addition, the presence of five putative virulence genes (ciaB, cadF, cj1349, hecA, and irgA) was screened for in these strains by PCR. All Arcobacter species except A. bivalviorum and Arcobacter sp. strain W63 adhered to Caco-2 cells, and most species (10/16) were invasive. The most invasive species were A. skirrowii, A. cryaerophilus, A. butzleri, and A. defluvii. All invasive strains were positive for ciaB (encoding a putative invasion protein). Other putative virulence genes were present in other species, i.e., A. butzleri (cadF, cj1349, irgA, and hecA), A. trophiarum (cj1349), A. ellisii (cj1349), and A. defluvii (irgA). No virulence genes were detected in strains which showed little or no invasion of Caco-2 cells. These results indicate that many Arcobacter species are potential pathogens of humans and animals

Nanoplastics: From tissue accumulation to cell translocation into Mytilus galloprovincialis hemocytes. resilience of immune cells exposed to nanoplastics and nanoplastics plus Vibrio splendidus combination

Plastic litter is an issue of global concern. In this work Mytilus galloprovincialis was used to study the distribution and effects of polystyrene nanoplastics (PS NPs) of different sizes (50 nm, 100 nm and 1 mu m) on immune cells. Internalization and translocation of NPs to hemolymph were carried out by in vivo experiments, while endocytic routes and effects of PS NPs on hemocytes were studied in vitro. The smallest PS NPs tested were detected in the digestive gland and muscle. A fast and size-dependent translocation of PS NPs to the hemolymph was recorded after 3 h of exposure. The internalization rate of 50 nm PS NPs was lower when caveolae and clathrin endocytosis pathways were inhibited. On the other hand, the internalization of larger particles decreased when phagocytosis was inhibited. The hemocytes exposed to NPs had changes in motility, apoptosis, ROS and phagocytic capacity. However, they showed resilience when were infected with bacteria after PS NP exposure being able to recover their phagocytic capacity although the expression of the antimicrobial peptide Myticin C was reduced. Our findings show for the first time the translocation of PS NPs into hemocytes and how their effects trigger the loss of its functional parameters

Kinematic groups across the MW disc: insights from models and from the RAVE catalogue

With the advent of the Gaia data, the unprecedented kinematic census of great part of the Milky Way disc will allow us to characterise the local kinematic groups and new groups in different disc neighbourhoods. First, we show here that the models predict a stellar kinematic response to the spiral arms and bar strongly dependent on disc position. For example, we find that the kinematic groups induced by the spiral arm models change significantly if one moves only ~ 0.6 kpc in galactocentric radius, but ~ 2 kpc in azimuth. There are more and stronger groups as one approaches the spiral arms. Depending on the spiral pattern speed, the kinematic imprints are more intense in nearby vicinities or far from the Sun. Secondly, we present a preliminary study of the kinematic groups observed by RAVE. This sample will allow us, for the first time, to study the dependence on Galactic position of the (thin and thick) disc moving groups. In the solar neighbourhood, we find the same kinematics groups as detected in previous surveys, but now with better statistics and over a larger spatial volume around the Sun. This indicates that these structures are indeed large scale kinematic features.Comment: 4 pages, 3 figures, to appear in the proceedings of "Assembling the Puzzle of the Milky Way", Le Grand Bornand (April 17-22, 2011), C. Reyle, A. Robin, M. Schultheis (eds.

GRChombo : Numerical Relativity with Adaptive Mesh Refinement

In this work, we introduce GRChombo: a new numerical relativity code which incorporates full adaptive mesh refinement (AMR) using block structured Berger-Rigoutsos grid generation. The code supports non-trivial "many-boxes-in-many-boxes" mesh hierarchies and massive parallelism through the Message Passing Interface (MPI). GRChombo evolves the Einstein equation using the standard BSSN formalism, with an option to turn on CCZ4 constraint damping if required. The AMR capability permits the study of a range of new physics which has previously been computationally infeasible in a full 3+1 setting, whilst also significantly simplifying the process of setting up the mesh for these problems. We show that GRChombo can stably and accurately evolve standard spacetimes such as binary black hole mergers and scalar collapses into black holes, demonstrate the performance characteristics of our code, and discuss various physics problems which stand to benefit from the AMR technique.Comment: 48 pages, 24 figure

A Low-Cost FPGA-Based Test and Diagnosis Architecture for SRAMs

The continues improvement of manufacturing technologies allows the realization of integrated circuits containing an ever increasing number of transistors. A major part of these devices is devoted to realize SRAM blocks. Test and diagnosis of SRAM circuits are therefore an important challenge for improving quality of next generation integrated circuits. This paper proposes a flexible platform for testing and diagnosis of SRAM circuits. The architecture is based on the use of a low cost FPGA based board allowing high diagnosability while keeping costs at a very low leve

The Black Di-Ring: An Inverse Scattering Construction

We use the inverse scattering method (ISM) to derive concentric non-supersymmetric black rings. The approach used here is fully five-dimensional, and has the modest advantage that it generalizes readily to the construction of more general axi-symmetric solutions.Comment: v3: 2 subsections added, typos fixed, more refs, journal version. v4: a transcription error in the ADM mass fixe